Sequence-Based Predictions Hemochromatosis

Hemochromatosis>>Task 3: Sequence-based predictions

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Short Task Description

Detailed description: Sequence-Based Predictions

• TODO: Task description
• TODO: Table numbers (once all tables are finished)

Protocol

Protocol

Disorder

<figtable id="iupred">

Table XXX: IUPred predictions for Q30201, P10775, Q08209, and Q9X0E6. The figures show the disorder probability predicted for each amino acid residue (green line) and the 50% threshold (red line).

	Q30201	P10775
	Q08209	Q9X0E6

</figtable>

IUPred was employed to find disordered regions within HFE (Q30201), RNH1 (P10775), PPP3CA (Q08209), and cutA (Q9X0E6). The results are shown in <xr id="iupred"/>. DisProt was used to validate the predictions.

Transmembrane Helices

<figtable id="tmh_q30201">

Table XXX: TMH predictions and annotations for Q30201. There were no entries for either of the two PDB IDs (1A6Z, 1DE4) in OPM or PDBTM.

Q30201	TMH 1
PolyPhobius	306-329
UniProt	307-330
OPM	no entry
PDBTM	no entry

</figtable>

<figtable id="tmh_p35462">

Table XXX: TMH predictions and annotations for P35462 (PDB ID: 3PBL).

P35462 (3PBL)	TMH 1	TMH 2	TMH 3	TMH 4	TMH 5	TMH 6	TMH 7
PolyPhobius	30-55	66-88	105-126	150-170	188-212	329-352	367-386
UniProt	33-55	66-88	105-126	150-170	188-212	330-351	367-388
OPM	34-52	67-91	101-126	150-170	187-209	330-351	363-386
PDBTM	35-52	68-84	109-123	152-166	191-206	334-347	368-382

</figtable>

<figtable id="tmh_p47863">

Table XXX: TMH predictions and annotations for P47863 (PDB ID: 2D57). TMH3 and TMH7 (marked with *) are listed as "Membrane Loop" in PDBTM.

P47863 (2D57)	TMH 1	TMH 2	TMH 3	TMH 4	TMH 5	TMH 6	TMH 7	TMH 8
PolyPhobius	34-58	70-91		115-136	156-177	188-208		231-252
UniProt	37-57	65-85		116-136	156-176	185-205		232-252
OPM	34-56	70-88	98-107	112-136	156-178	189-203	214-223	231-252
PDBTM	39-55	72-89	95-106*	116-133	158-177	188-205	209-222*	231-248

</figtable>

<figtable id="tmh_q9ydf8">

Table XXX: TMH predictions and annotations for Q9YDF8 (PDB IDs: 1ORQ, 1ORS). Residue positions are adjusted for the PDB sequence's 13AA shift. TMH3 is annotated as "Intramembrane, Helical" in UniProt, TMH7 as "Intramembrane, Pore-Forming". TMH7 is additionally marked as "Membrane Loop" in PDBTM.

Q9YDF8 (1ORQ/1ORS)	TMH 1	TMH 2	TMH 3	TMH 4	TMH 5	TMH 6	TMH 7	TMH 8
PolyPhobius	42-60	68-88		108-129	137-157	163-184	196-213	224-244
UniProt	39-63	68-92	97-105*	109-125	129-145	160-184	196-208*	222-253
OPM (1ORS)	38-59	68-91	99-110	113-120	130-161
OPM (1ORQ)						166-185	196-208	220-238
PDBTM (1ORS)	40-63	68-88		101-120	131-155
PDBTM (1ORQ)	34-65	70-93				164-184	197-213*	222-249

</figtable>

Signal Peptides

<figtable id="signalp">

Table XXX: SignalP predictions for Q30201, P47863, P11279, and P02768. Each figure shows the C-score, S-score, and Y-score per residue position for the corresponding protein.

	Q30201	P47863
	P11279	P02768

</figtable>

TODO: score description

SignalP (Webserver 4.0) predictions were made for HFE (Q30201), Aquaporin-4 (P47863), Lysosome-associated membrane glycoprotein 1 (P11279), and Serum albumin (P02768) in order to find signal peptides within these sequences. The results are shown in <xr id="signalp"/> and were compared to the corresponding entries in UniProt.

According to UniProt all four predictions are 100% precise:

• Q30201: signal peptide 1-22
• P47863: no signal peptide
• P11279: signal peptide 1-28
• P02768: signal peptide 1-18

This makes SignalP an excellent candidate for signal peptide predictions.

GO Terms

For the last part of this task we used GOPET and ProtFun to make a GO term prediction for the HFE protein (Q30201). We did also search for Pfam families. The results were then compared to UniProt and QuickGO.

GOPET

GOPET predicts only two GO terms for our protein (see <xr id="gopet"/>) and even they are somewhat redundant (both are receptor activity). At least the results are correct in that HFE has kind of a receptor activity in that it binds to transferrin receptor (TFR).

<figtable id="gopet">

Table XXX: GO term prediction with GOPET for Q30201.

GOid	Aspect	Confidence	Go term
GO:0004872	F (Molecular Function Ontology)	91%	receptor activity
GO:0030106	F (Molecular Function Ontology)	88%	MHC class I receptor activity

</figtable>

ProtFun

The results for the ProtFun prediction are shown in <xr id="protfun"/>. Predictions with a probability below 0.1 and odds below 1.0 are not shown to decrease the size of the table. ProtFun predicts "cell envelope" for the functional category. This is true as the HFE-TFR complex is located in the membrane. "Transport and binding" also has a high probability which corresponds with HFE's part in the iron transport within the body. HFE is categorized as "Nonenzyme" and no enzyme class was predicted. It is further predicted to be involved in "Immune response" as it is a protein of the major histocompatibility complex (MHC) class I.

<figtable id="protfun">

Table XXX: GO term prediction with ProtFun for Q30201. Entries marked with asterisks (*) had been deemed "true" by ProtFun. Results with a probability below 0.1 and odds below 1.0 are not shown.

Functional category	Probability	Odds
Biosynthesis of cofactors	0.105	1.452
Cell envelope*	0.633*	10.377*
Cellular processes	0.095	1.297
Central intermediary metabolism	0.231	3.663
Fatty acid metabolism	0.016	1.265
Purines and pyrimidines	0.583	2.400
Translation	0.079	1.801
Transport and binding	0.732	1.785
Enzyme/nonenzyme
Enzyme	0.208	0.727
Nonenzyme*	0.792*	1.110*
Enzyme class
Hydrolase	0.135	0.425
Lyase	0.049	1.054
Gene Ontology category
Signal transducer	0.201	0.939
Receptor	0.353	2.076
Stress response	0.274	3.108
Immune response*	0.381*	4.486*

</figtable>

Pfam

Pfam lists two significant results for Q30201:

• MHC_I - Class I Histocompatibility antigen, domains alpha 1 and 2 (E-value 3.5e-43)
• C1-set - Immunoglobulin C1-set domain (E-value 2.8e-18)

MHC class I proteins are strongly involved in immune responses. UniProt also lists HFE in the MHC class I family and its structure (three extracellular domains, transmembrane region, cytoplasmic tail) fits. C1-set domains are associated with MHC class I proteins and HFE indeed contains such a domain (residues 207-298)

Comparison

Compared to QuickGO which lists 27 unique GO terms for Q30201, GOPET predicts only two. Both of them not included in QuickGO's list. These two also seem to fit the HFE-TFR complex better than HFE alone, but at least the MHC class I tag is shows specificity to HFE.

ProtFun's prediction seems more accurate as it successfully identifies HFE's location within the membrane and lists "Transport and binding" as a good second result. "Immune response" is also in accordance to QuickGO's term.

Pfam's two predicted families were both true positives and it was more informative that the other two methods.

Overall none of them did identify HFE's part in the iron transport.