Phenylketonuria

Summary

Phenylketonuria (PKU) is an inborn metabolic disorder and is caused by different mutations in the enzyme phenylalanine hydroxylase (PAH), which is responsible for the alteration of phenylalanine to tyrosine. Untreated PKU can lead to psychotic disorders and the postnatal development of the brain can be harmed.

Phenotype

PKU results in enrichment of phenylalanine and decrease of tyrosine, whereby other features of the phenylalanine metabolism pathway are affected like the production of melanin. Thus symptoms like fair skin, light hair and blue or sometimes even red eyes can occur. Additionally there can be mousy odour, eczema, epilepsy and perceptible posture changes. In some cases the concerned persons show psychotic disorders, ADHD (attention-deficit hyperactivity disorder), Aggressions, autistic features, Hyperactivity and Self-harm (Self-mutilation).

There are two different PKU variants defined:

• Classical Phenylketonuria is caused by mutation/s in the PAH gene (precisely described here)
• Atypical (mild) Phenylketonuria is a deficiency of the tetrahydrobiopterin (BH4), a cofactor of the enzyme phenylalanine hydroxylase

Cross-references

See also description of this disease in

• OMIM
• Wikipedia
• PubMed Health
• KEGG
• GeneReviews
• GeneCards
• PubMed
• Genetics Home Reference

Treatment

After birth every child is tested with a newborn-screening, where some disorders are analysed. If a higher concentration of phenylalanine or a bad ratio of phenylalanine and tyrosine is found, PKU is diagnosed and the patient can be treated as followed:

• Special low phenylalanine diet for the whole life (routine treatment; constrains in meat, fish, eggs, nuts, legumes and milk products and careful usage in starchy food)
• Tyrosine enriched food or medicaments
• Intake of the enzyme PAL (phenylalanine ammonia lyase), which converts phenylalanine to a harmless metabolite
• Tetrahydrobiopterin (only for mild PKU!!!)

Cross-references

• OMIM
• Wikipedia

Biochemical disease mechanism

• The symptoms of PKU are caused by the complete or partial defected catalysing of phenylalanine to tyrosine.
• As it occurs at the beginning of the phenylalanine metabolism pathway other metabolic diseases are associated like Albinism or Cretinism.
• PKU was named after the phenylketone (phenylpyruvate) in which phenylalanine is converted.
• Protein phenylalanine hydroxylase:
  • is expressed in liver,
  • has a sequence length of 452 aa,
  • is encoded by the gene PAH (see Genomic information) on chromosome 12.

Mutations and Genomic information

Genomic information

Chromosome 12: red box indicates location of the PAH gene (Ensembl)

PKU is an autosomal recessive disorder which can be caused by 400 different known mutations on the PAH gene. This gene lies on the human chromosome 12 and consists of 13 exons. Most common are missense mutations, but also nonsense and silent mutations can be found. Normally the enzyme phenylalanine hydroxylase converts phenylalanine into tyrosine. If this pathway is disturbed, it is transformed to phenylpyruvic acid.

Hydroxylation of phenylalanine to tyrosine and penylpyruvic acid (Davidson College)

Reference sequence

The Uniprot entry for PAH P00439 gives the protein sequence used as reference in this practical:

>sp|P00439|PH4H_HUMAN Phenylalanine-4-hydroxylase OS=Homo sapiens GN=PAH PE=1 SV=1

MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDV NLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPW FPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYM EEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGF RLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFA QFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSE KPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQR IEVLDNTQQLKILADSINSEIGILCSALQKIK