Difference between revisions of "Task3 Hemochromatosis Protocol"

From Bioinformatikpedia
(Secondary Structure)
(Disorder)
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=== Disorder ===
 
=== Disorder ===
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  +
IUPred predictions:
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<source lang="bash">
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#!/bin/bash
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#Disorder
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DIS="/mnt/home/student/bernhoferm/mstrprkt/task3/disorder"
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cd /opt/iupred/
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iupred $DIS/fasta/Q30201.fasta long > $DIS/pred/Q30201.pred
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iupred $DIS/fasta/P10775.fasta long > $DIS/pred/P10775.pred
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iupred $DIS/fasta/Q9X0E6.fasta long > $DIS/pred/Q9X0E6.pred
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iupred $DIS/fasta/Q08209.fasta long > $DIS/pred/Q08209.pred
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</source>
   
 
=== Transmembrane Helices ===
 
=== Transmembrane Helices ===

Revision as of 12:00, 17 May 2012

Data Acquisition

Retrieve all sequences: <source lang="bash">

  1. !/bin/bash
  1. Secondary Structure

cd /mnt/home/student/bernhoferm/mstrprkt/task3/ss/fasta/

wget http://www.uniprot.org/uniprot/Q30201.fasta wget http://www.uniprot.org/uniprot/P10775.fasta wget http://www.uniprot.org/uniprot/Q9X0E6.fasta wget http://www.uniprot.org/uniprot/Q08209.fasta


  1. Disorder

cd /mnt/home/student/bernhoferm/mstrprkt/task3/disorder/fasta/

wget http://www.uniprot.org/uniprot/Q30201.fasta wget http://www.uniprot.org/uniprot/P10775.fasta wget http://www.uniprot.org/uniprot/Q9X0E6.fasta wget http://www.uniprot.org/uniprot/Q08209.fasta


  1. Transmembrane Helices

cd /mnt/home/student/bernhoferm/mstrprkt/task3/tmh/fasta/

wget http://www.uniprot.org/uniprot/Q30201.fasta wget http://www.uniprot.org/uniprot/P35462.fasta wget http://www.uniprot.org/uniprot/Q9YDF8.fasta wget http://www.uniprot.org/uniprot/P47863.fasta


  1. Signal Peptides

cd /mnt/home/student/bernhoferm/mstrprkt/task3/sp/fasta/

wget http://www.uniprot.org/uniprot/Q30201.fasta wget http://www.uniprot.org/uniprot/P02768.fasta wget http://www.uniprot.org/uniprot/P47863.fasta wget http://www.uniprot.org/uniprot/P11279.fasta </source>

Secondary Structure

ReProf predictions: <source lang="bash">

  1. !/bin/bash
  1. Secondary Structure

cd /mnt/home/student/bernhoferm/mstrprkt/task3/ss/fasta/

reprof -i Q30201.fasta -o ../pred/ &> ../Q30201.log reprof -i P10775.fasta -o ../pred/ &> ../P10775.log reprof -i Q9X0E6.fasta -o ../pred/ &> ../Q9X0E6.log reprof -i Q08209.fasta -o ../pred/ &> ../Q08209.log </source>

Disorder

IUPred predictions: <source lang="bash">

  1. !/bin/bash
  1. Disorder

DIS="/mnt/home/student/bernhoferm/mstrprkt/task3/disorder" cd /opt/iupred/

iupred $DIS/fasta/Q30201.fasta long > $DIS/pred/Q30201.pred iupred $DIS/fasta/P10775.fasta long > $DIS/pred/P10775.pred iupred $DIS/fasta/Q9X0E6.fasta long > $DIS/pred/Q9X0E6.pred iupred $DIS/fasta/Q08209.fasta long > $DIS/pred/Q08209.pred </source>

Transmembrane Helices

Signal Peptides

GO Terms