Sequence-based analyses Gaucher Disease

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Secondary structure

Knowing the secondary structure of a protein can shed light on its function since structure implies function. If the structure of a protein is known, secondary structure elements (helix, sheet, coiled) can be assigned to its residues depending on their affinity to form hydrogen bonds. DSSP <ref name="DSSP">Kabsch W, Sander C (1983). "Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features". Biopolymers</ref> is the most common method to perform such secondary structure assignments. If the structure of a protein is unknown, secondary structure elements be be predicted by tools like PSIPRED <ref name="PSIPRED">Liam J. McGuffin, Kevin Bryson, and David T. Jones (2000). "The PSIPRED protein structure prediction server". Bioinformatics</ref> or Reprof<ref name="Reprof">B Rost, C Sander (1993). "Prediction of protein secondary structure at better than 70% accuracy". J. Mol. Bio.</ref>. The aim of this task was to analyse the secondary structure of different proteins and the compare the secondary structure predictions of PSIPRED and Reprof with the DSSP secondary structure assignments. Following sequences were taken into account: <figtable id="tab:ss_sequences">

NAME UniProtKB PDB
Glucosylceramidase P04062 1OGS
Ribonuclease inhibitor P10775 1DFJ
Divalent-cation tolerance protein CutA Q9X0E6 1KR4
Serine/threonine-protein phosphatase Q08209 1AUI

</figtable> Information about program calls and implementation details can be found in our protocol.

Predictions

For being able to better compare the different output formats, we mapped the secondary structure definitions of all three methods onto the three letters H (helix), E (sheet), and C (coiled) according to table <xr id="tab:ss_mapping"/>. Regions of the UniProt sequences which were not present in the PDB file as well as regions where no DSSP assignment was possible were ignored. <figtable id="tab:ss_mapping">

Method H E C
DSSP H,G,I E,B T,S,' '
PSIPRED H E C
Reprof H E L

</figtable>


P04062

Glycosylcermidase (P04062) is located the the membrane of lysosomes. It exhibits two domains which belong to the (1) glycosyl hydrolase domain fold and (2) the TIM beta/alpha-barrel fold. Both domains have hydrophobic beta sheets which anchor the protein in the membrane. <xr id="fig:ss_P04062"/> depicts the secondary structure elements of the corresponding crystal structure which coincide with the DSSP assignments. The following section shows the secondary structure annotations of the different methods: The PSIPRED predictions better coincide with the DSSP assigments than the Reprof predictions do. Reprof predicts sheets instead of helices in several regions. The residues of the beta-barell sheets (the tube in the middle of <xr id="fig:ss_P04062"/>) are marked by asterisks.

</figure>

          40
          ARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKG
Reprof    CCCCCCCCCCCEEEEEEECCEECCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCEECCCCCCEEEEEECCCCEEEEEEC
PSIPRED   CCCCCCCCCCCCCEEEEECCHHCCCCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCEEEECCCCCCEEEEE
DSSP      CECCCEEECCCCCEEEEEECCCCCECCCCCCCCCCEEEEEEEECCCCCCEEEEEECECCCCCCCEEEEEEEEEEEEECCE

          120
          FGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPL
Reprof    CCCCCCHHHHHEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCEEEEECCCCCCCCCEEEE
PSIPRED   EEEEHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DSSP      EEEECCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHH

          200
          IHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGL
Reprof    EEHHHHHCCCCCEEEECCCCCCCEEEECCCECCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCE
PSIPRED   HHHHHHHHCCCEEEEEEECCCCHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCC
DSSP      HHHHHHHCCCCCEEEEEECCCCHHHECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHH
                      ******                                                  ***
          280
          LSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPA
Reprof    ECCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEECCCEEEEEECCCCCCEEEEEEEEEEEEEECCCC
PSIPRED   CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHCCEEEEECCCCCCCCH
DSSP      CCCCCCCCCECCHHHHHHHHHHCHHHHHHCCCCCCCEEEEEEEEHHHCCHHHHHHHCCHHHHCCCCEEEEEEECCCCCCH
                                              ********                      ******* 
          360
          KATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDS
Reprof    CCECCCCCECCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEECEHHHHEEEEEEECCCCEEEECCCCCCEEEEECCCC
PSIPRED   HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCC
DSSP      HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEECCECCCCCCCCCCCCCCC
                        ********                                ******** 
          440
          PIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFL
Reprof    CEEEEECCCCCCCCCEEEECCCEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCEEEE
PSIPRED   CEEEECCCCEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCEEE
DSSP      CEEEEHHHCEEEECHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEEEECCCEEE

          520
          ETISPGYSIHTYLWRRQ
Reprof    EEECCCCEEEEEEEECC
PSIPRED   EEECCCCEEEEEEEECC
DSSP      EEEECCCEEEEEEECCC

<figure id="fig:ss_P04062">

Crsytal structure of 1OGS (P04062). Red: alpha-helix, yellow: beta-sheet, green: coiled.


P10775

<xr id="fig:ss_P10775"/> depicts the crystal structure 1DFJ which refers to P10775. It has two domains: d1dfji_ is a repeat domain consisting of altering alpha-helices and parallel beta-sheets. d1dfje_ contains long curved antiparallel beta-sheets and three alpha-helices. The alternating HHH and EEE regions in the following secondary structure annotations suit well with repetitive structure shown in <xr id="fig:ss_P10775"/>. Again, the PSIPRED predictions better match the DSSP assignments than Reprof.

</figure>

          1
          MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTC
Reprof    CCCCECHHCCCCCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHEEEHCCCCCCCC
PSIPRED   CEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCC
DSSP      CECCCECCCCCHHHHHHHHHHHCCCCEEECECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCC

          81
          KIQKLSLQNCSLTEAGCGVLPSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASV
Reprof    EEEEECCCCCCCCHCCCCCCHHHHCHCHHHHHHCCCCCCCCHHHHHHHHHCCCCCHCCHHHHHHHHHHCCCCCCHHHHHH
PSIPRED   CCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHH
DSSP      CCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHH

          161
          LRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLRLENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIA
Reprof    HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHCCHHHCCCCCCCCCHHHHH
PSIPRED   HHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHH
DSSP      HHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHH

          241
          ELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLCESLLQPGCQLESLWVKSCSL
Reprof    HHCCCCCCCHHHHCHHEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCHH
PSIPRED   HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCC
DSSP      HHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCC

          321
          TAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL
Reprof    HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHCC
PSIPRED   CHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEC
DSSP      EHHHHHHHHHHHHHCCCCCEEECCCCECHHHHHHHHHHHHHCCCCCCCEEECCCCCCEHHHHHHHHHHHHHCCCCCEEEC

          401
          SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS
Reprof    CCCCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCECC
PSIPRED   CCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECC
DSSP      CCCECCHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEC

<figure id="fig:ss_P10775">

Crsytal structure of 1DFJ (P10775). Red: alpha-helix, yellow: beta-sheet, green: coiled.

Q9X0E6

<xr id="fig:ss_Q9X0E6"/> depicts the d1kr4a_ domain of 1KR4 which is made of three alpha-helices interrupted by beta-sheets. Reprof predicts too long helices.

</figure>

          2
          ILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIF
Reprof    EEEEECCCCHHHHHHHHHHHHHHHHHHHHCHCHHHCCCEEECEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHE
PSIPRED   EEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEE
DSSP      EEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCEEEEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEE

          82
          TLKVENVLTEYMNWLRESVL
Reprof    HHHHHHHHHHHHHHHHHHCC
PSIPRED   EEECCCCCHHHHHHHHHHCC
DSSP      EECCCCEEHHHHHHHHHHCC

<figure id="fig:ss_Q9X0E6">

Crsytal structure of 1KR4 (Q9X0E6). Red: alpha-helix, yellow: beta-sheet, green: coiled.

Q08209

Q08209 contains the domain d1auib_ and d1auia_ which are mainly assembled of alpha-helices (<xr id="fig:ss_Q08209"/>). PSIPRED predicts these alpha-helices considerably better than Reprof which suggests beta-sheets in some regions.

</figure>

          14
          TDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHG
Reprof    CCCEEEEECCCCCCCEEEEEEECCCCCCEEEEEHHHECCCCCCCEEEEEEEEECCCCEEECCCCCCCCCCCEEEEECCCC
PSIPRED   CCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCC
DSSP      CCCCCCCCCCCCCCCECHHHHECCCCCECHHHHHHHHHCCCCECHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCC

          94
          QFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSER
Reprof    HHHHHHEEEEECCCCCCCEEEEEEEECCCCEEEEEEEHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEEEEEEEEECH
PSIPRED   HHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCHHHHHHHHCCHH
DSSP      CHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHCCHHHHHHHHCCHH

          174
          VYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTV
Reprof    HHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCECCCCCE
PSIPRED   HHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
DSSP      HHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCEECCCCCCCCCCCCEEECCC

          254
          RGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQ
Reprof    CCEEEEECCCCEEEEHCCCCHHHHEHHHCCCCCCEEEEEECCCCCCCEEEEEEECCCEEEEECCCEEEEEECCCEEEEEE
PSIPRED   CCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCEEEE
DSSP      CCCCEEECHHHHHHHHHHCCCCEEEECCCCCCCCEEECCECCCCCCECEEEECCCCCHHHCCCCCEEEEEEECCEEEEEE

          334
          FNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSSFEEAKGLDRINERMPPR
Reprof    ECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHEECCEHHHHCCCCHHCCCCCCC
PSIPRED   ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
DSSP      ECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC

<figure id="fig:ss_Q08209">

Crsytal structure of 1AUI (Q08209). Red: alpha-helix, yellow: beta-sheet, green: coiled.

Prediction accuracy/precision

We compared the prediction performance of PSIPRED and Reprof via the Q3 score and the precision of the three secondary structure states H,E, and C. The Q3 score is identical to the accuracy, i.e. the number of correctly predicted states divided by the length of the protein. The precision of state X is the fraction of correct predictions of X, formally precision(X)=TP(X)/(TP(X)+FP(X)). <xr id="ss_acc"/> shows the results: PSIPRED clearly outperforms Reprof in all for cases. PSIPRED achieves an average accuracy of 87% which is significantly higher than 58% in case of Reprof. <figtable id="ss_acc">

Method Q3 Precision H Precision E Precision C
P04062
PSIPRED 0.831 0.830 0.872 0.810
Reprof 0.553 1.000 0.455 0.592
P10775
PSIPRED 0.941 0.959 0.960 0.919
Reprof 0.603 0.589 0.417 0.644
Q9X0E6
PSIPRED 0.890 1.000 0.895 0.720
Reprof 0.580 0.562 0.917 0.458
Q08209
PSIPRED 0.833 0.842 0.902 0.812
Reprof 0.579 0.762 0.293 0.743

</figtable>

Disorder

Disordered regions are regions with a varying three-dimensional structure. Nevertheless, such regions can be functionally highly important: regulation, signalling, and flexible ligand binding are only some examples. DisProt<ref name="DisProt">Vucetic S, Obradovic Z, Vacic V, et al. (2005). "DisProt: a database of protein disorder". Bioinformatics</ref> is a curated databases of proteins with experimentally determined disordered regions. IUPred<ref name="IUPred">Zsuzsanna Dosztányi, Veronika Csizmók, Péter Tompa and István Simon (2005). "The Pairwise Energy Content Estimated from Amino Acid Composition Discriminates between Folded and Intrinsically Unstructured Proteins". J. Mol. Biol.</ref> is a method for predicting disordered regions ab-initio, i.e. based solely on the protein sequence. We compared the predictions of IUPred with the annotations in the DisProt database for all four example proteins. IUPred was called to predict long, global disorder regions (confer the protocol for details). Residues involved in disordered regions were defined as those with a probability of at least 50%. These residues were compared to the DisProt annotations: either by the UniProt entry itself if available, or by a significant homolog (e-value < 1e-3) for which a DisProt entry existed. We measured the performance of IUPred via the precision (TP/(TP+FP)), sensitivity (TP/(TP+FN)), and specificity (TN/(TN+FP)).

P04062

Neither Glycosylceramidase (P04062) nor a homologous protein is annotated in DisProt. This might be due to lacking experimental data or, which is more likely, due to lacking disordered regions. The latter assumption is supported by the highly structured protein complex 1OGS (<xr id="fig:ss_P04062"/>). However, IUOred predicts some disordered residues with a probability >= 50%.

<figtable id="disorder_P04062">

Method Disorder regions
IUPred 2, 3, 6, 90-93, 229-231, 235, 236
DisProt
Precision: 0% Sensitivity: undef Specificity: 98%

</figtable>

P10775

P10775 is not annotated in DisProt itself, but there is a significant homolog (DP00554) with a disordered region from 31 to 50. This region is, however, is not covered by the pairwise alignment of P10775 and DP00554. Hence, IUpred correctly did not predict any disordered region (Specificity=100%).

Q9X0E6

There is neither an entry in DisProt which suggests a disordered region in Q9X0E6 and nor does IUPred predict such a region.

Q08209

Five disordered regions are annotated in DisProt for Q08209. These regions mainly cover the C-terminal end which exhibits several rather arbitrarily arranged alpha-helices (<xr id="fig:ss_Q08209"/>). All residues predicted by IUPred with a probability >= 50% are covered by DisProt annotations (precision 100%), but IUpred predicts only about half of all disordered regions (sensitivity 52%).

</figure>

<figtable id="disorder_Q08209">

Method Disorder regions
IUPred 1-6, 8, 383,384,424,425,432,434-439,443,445,448,449,455,458,463-521
DisProt 1-13,390-414,374-468,469-486,487-521
Precision: 100% Sensitivity: 52% Specificity: 100%

</figtable>

<figure id="fig:disorder_Q08209">

Disordered and ordered regions of Q08209 using the DisProt entry DP00092.

Transmembrane helices

PolyPhobius was used to predict transmembrane helices for our protein P04062 and other three proteins P35462, Q9YDF8 and P47863. The scripts which were used to do the prediction can be found here [protocal]. The prediction results from PolyPhobius were then compared with the membrane assignment of the structures for these proteins in OPM and/or PDBTM. For that purpose, a corresponding pdb structure for each protein was needed and was taken from uniprot(<xr id="tab:ss_sequences_trans"/>):

<figtable id="tab:ss_sequences_trans">

NAME UniProtKB PDB
Glucosylceramidase P04062 1OGS
D(3) dopamine receptor P35462 3PBL
DVoltage-gated potassium channel Q9YDF8 1ORQ 1ORS
Aquaporin-4 P47863 2D57

</figtable>
Table 6: The proteins used to predict transmenbrane helix.

P04062

Our protein Glucosylceramidase is located in the lysosome, therefore contains no transmembrane regions. As expected, PolyPhobius has not reported any transmembrane regions. Instead, the most regions were predicted lying in the non-cytoplam and a signal peptide was found. Compared with that in uniprot, PolyPhobius has returned correct prediction (<xr id="tab:P04062_trans"/>):

<figtable id="tab:P04062_trans">

P04062 Signal peptide Others
PolyPhobius 1-39 40-536 NON CYTOPLASMIC
Uniprot 1-39 40–536 Chain

</figtable>

Table 7: Transmenbrane helix prediction results for P04062.

P35462

For the protein "dopamine receptor( P35462, )", only one pdb structure(3PBL) was found in uniprot. All three prediction tools PolyPhobius, OPM and PDBTM returned 7 regions of transmenbrane helix, which were confirmed by uniport. In <xr id="tab:P35462_trans"/> we can see that even the start and stop position of such regions showed very little deviation (maximally 4 bases). Figure 6 and 7 showed us the visualization of transmenbrane helix in OPM and PDBTM.

<figtable id="tab:P35462_trans">

P35462 TRANSMEM 1 TRANSMEM 2 TRANSMEM 3 TRANSMEM 4 TRANSMEM 5 TRANSMEM 6 TRANSMEM 7
PolyPhobius 30-55 66-88 105-126 150-170 188-212 329-352 367-386
Uniprot 33–55 66–88 105–126 150–170 188–212 330–351 367–388
OPM 34-52 67-91 101-126 150–170 187-209 330-351 363-386
PDBTM 35-52 68-84 109-123 152-166 191-206 334-347 368-382

</figtable>


Table 8: Transmenbrane helix prediction results for P35462.



Q9YDF8

For the protein KvAP("Voltage-gated potassium channel,Q9YDF8)", since no single pdb structure was found to cover the whole sequence, two pdb structures (1ORQ,1ORS) were used. Not like what happened to the previous protein, the prediction results varied among different prediction tools(<xr id="tab:Q9YDF8_trans"/>).

PolyPhobius returned the closest prediction as compared to that from uniprot. It reported one transmenbrane region less. By using two pdb structures, OPM has returned all the transmenbrane regions as those listed in uniprot, however the most of them showed quited big deviation of the start and stop positions. Although PolyPhobius reported one transmenbrane region less, the most showed much more accurate prediction of position. In PDBTM, even two pdb structures were used, still two regions were missed.

<figtable id="tab:Q9YDF8_trans">

Q9YDF8 TRANSMEM 1 TRANSMEM 2 TRANSMEM 3 TRANSMEM 4 TRANSMEM 5 TRANSMEM 6 TRANSMEM 7 TRANSMEM 8
PolyPhobius 42-60 68-88 - 108-129 137-157 163-184 196-213 224-244
Uniprot 39–63 68–92 97–105(Intramembrane) 109–125 129–145 160–184 196–208(Intramembrane) 222 – 253
OPM_1ORQ - - - - - 153-172 183-195 207-225
OPM_1ORS 25-46 55-78 86-97 100-107 117-148 - - -
PDBTM_1ORQ 21-52 57-80 - - 151-171 - - 209-236
PDBTM_1ORS 27-50 55-75 88-107 118-142 - - - -

</figtable>


Table 10: Transmenbrane helix prediction results for Q9YDF8.



P47863

For the protein Aquaporin-4 ("Mercurial-insensitive water channel,P47863 )",the pdb structures 2D57 from X-ray with relative higher resolution was chosen. The prediction results did not vary too much among different prediction tools(<xr id="tab:P47863_trans"/>).

Not like PolyPhobius and that stated in uniprot, OPM reported two additional transmenbrane regions. PDBTM also returned two similar regions which were recognized as "Membrane loop". At transmembrane region 2, all three prediction tools showed an at least 5 bases leftward shift.

<figtable id="tab:P47863_trans">

P47863 TRANSMEM_1 TRANSMEM_2 TRANSMEM_3 TRANSMEM_4 TRANSMEM_5 TRANSMEM_6 TRANSMEM_7 TRANSMEM_8
PolyPhobius 34-58 70-91 - 115-136 156-177 188-208 - 231-252
Uniprot 37–57 65–85 - 116–136 156–176 185–205 - 232–252
OPM 34-56 70-88 98-107 112-136 156-178 189-203 214-223 231-252
PDBTM 39-55 72-89 95-106 (Membrane loop) 116-133 158-177 188-205 209-222 (Membrane loop) 231-248

</figtable>


Table 12: Transmenbrane helix prediction results for P47863.



Signal peptides

The online server of SignalP with version 4.0 was used to predict signal peptides for our protein P04062 and other three proteins P02768, P47863 and P11279(<xr id="tab:ss_sequences_signa"/>): We also used Polyphobius and the information from uniprot and Signal Peptide Website to compare and validate the prediction results.

SignalP used three different scores, C, S and Y. Two additional scores, S-mean and the D-score, are reported in the output. The algorithm of SignalP employed two different neural networks, one for predicting the actual signal peptide and one for predicting the position of the signal peptidase cleavage site.

  • The S-score is reported for every single amino acid position. If S-score is high, it suggests that the corresponding amino acid is part of a signal peptide. On the other hand, a low score indicate that the amino acid is part of a mature protein.
  • The C-score is so called cleavage site score which should only be significantly high at the cleavage site.
  • The Y-max is calculated by combining the C-score with the S-score and produces a better cleavage site prediction than the raw C-score alone.
  • The S-mean is the average of the S-score for the length of the predicted signal peptide.
  • The D-score is calculated as a weighted average of the S-mean and the Y-max scores.

Observing the above scores will help us to locate the signal peptides.


<figtable id="tab:ss_sequences_signa">

NAME UniProtKB PDB
Glucosylceramidase P04062 1OGS
Serum albumin P02768 1AO6
Aquaporin-4 P47863 2D57
Lysosome-associated membrane glycoprotein 1 P11279 -

</figtable>


Table 11: The proteins used to predict signal peptides.


P04062

By using the standard option, SignalP did not return a positive result for our protein Glucosylceramidase P04062, even the s score and c score were quite high. After lowering the Cutoff value to 0.35, SignalP returned a finding of signal peptide which was validated by other tools and database(<xr id="tab:ss_sinal_p04062"/>).

# Measure  Position  Value    Cutoff   signal peptide?
  max. C    40       0.305
  max. Y    40       0.396
  max. S    36       0.684
  mean S     1-39    0.323
       D     1-39    0.367   0.350   YES
  Name=sp_P04062_GLCM_HUMAN	      SP='YES' Cleavage site between pos. 39 and 40: ASG-AR D=0.367 D-cutoff=0.350 Networks=SignalP-TM

<figtable id="tab:ss_sinal_p04062">

P04062 position
SignalP 1-39
Signal Peptide Website 1-39
uniprot 1-39
PolyPhobius 1-39
signal peptide sequence MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG

</figtable>


Table 12: Signal peptides prediction of P04062.

P02768

For the protein Serum albumin (P02768), SignalP returned positive prediction which was validated by using other tools.

# Measure  Position  Value    Cutoff   signal peptide?
  max. C    19       0.710
  max. Y    19       0.798
  max. S     2       0.929
  mean S     1-18    0.890
       D     1-18    0.848   0.450   YES
Name=sp_P02768_ALBU_HUMAN	SP='YES' Cleavage site between pos. 18 and 19: AYS-RG D=0.848 D-cutoff=0.450 Networks=SignalP-noTM

<figtable id="tab:ss_sinal_p02768">

P02768 position
SignalP 1-18
Signal Peptide Website 1-18
uniprot 1-18
PolyPhobius 1-18
signal peptide sequence MKWVTFISLLFLFSSAYS

</figtable>


Table 13: Signal peptides prediction of P02768.

P47863

For the protein Aquaporin-4 P47863, SignalP returned very low scores for which it was not meaningful to lower the cutoff value. All the other tools reported no finding either (<xr id="tab:ss_sinal_P47863"/>).

# Measure  Position  Value    Cutoff   signal peptide?
  max. C    41       0.209
  max. Y    41       0.164
  max. S    56       0.183
  mean S     1-40    0.139
       D     1-40    0.154   0.500   NO
  Name=sp_P47863_AQP4_RAT	  SP='NO' D=0.154 D-cutoff=0.500 Networks=SignalP-TM

<figtable id="tab:ss_sinal_P47863">

P47863 position
SignalP -
Signal Peptide Website -
uniprot -
PolyPhobius -
signal peptide sequence -

</figtable>


Table 14: Signal peptides prediction of P47863.

P11279

For the protein Lysosome-associated membrane glycoprotein 1 (P11279), a long signal peptide was found with very high score:

 # Measure  Position  Value    Cutoff   signal peptide?
   max. C    29       0.910
   max. Y    29       0.939
   max. S    21       0.996
   mean S     1-28    0.962
        D     1-28    0.952   0.450   YES
   Name=sp_P11279_LAMP1_HUMAN	   SP='YES' Cleavage site between pos. 28 and 29: ASA-AM D=0.952 D-cutoff=0.450 Networks=SignalP-noTM

<figtable id="tab:ss_sinal_P11279">

P11279 position
SignalP 1-28
Signal Peptide Website 1-28
uniprot 1-28
PolyPhbius 1-28
signal peptide sequence MAAPGSARRPLLLLLLLLLLGLMHCASA

</figtable>


Table 15: Signal peptides prediction of P11279.


GO terms

GOPET

GOPET was used to predict GO terms for our protein Glucosylceramidase (P04062). The results was shown in <xr id="tab:ss_gopet"/> where only three GO terms were returned. Resetting the search options like lower the Confidence threshold did not change the prediction results.
All three GO terms belong to "Molecular Function Ontology" and are all involved in "hydrolase" or "glycosyl bonds". Since we have already known the function of our protein Glucosylceramidase (P04062) is to cleaves the glucosidic bonds of glucosylceramide and synthetic beta-glucosides. All these three predicted GO terms are correct. However, if compared with the GO terms list from uniprot(QuickGo) or the prediction from ProtFun which is shown below, GOPET returned quite fewer results. It implies that GOPET prefers to report only the GO terms with high confidence.

<figtable id="tab:ss_gopet">

GOid Aspect Confidence GO term validate in QuickGo
GO:0016787 F 98% hydrolase activity yes
GO:0004348 F 97% glucosylceramidase activity yes
GO:0016798 F 97% hydrolase activity acting on glycosyl bonds yes

</figtable>
Table 16: GO terms prediction results for P04062 in GOPET.


ProtFun

ProtFun2.0 was also used to predict GO terms for our protein Glucosylceramidase (P04062). The results were listed in <xr id="tab:ss_protfun"/>. In the table, for each predicted terms, there are two scores. The first one is the estimated probability that the entry belongs to the class.It is influenced by the prior probability of that class. The second number represents the odds that the sequence belongs to that class/category. It is independent of the prior probability.
Compared with GOPET which reported only GO identifier, ProtFun returned much more information about the input protein:
At first, the input protein was classified into 12 different functional categories based on the scheme developed by Monica Riley for E. coli in 1993<ref name="protfun_scheme">Monica Riley (1993). "Functions of the gene products of Escherichia coli.". Microbiol Rev[1]</ref>. As we can see in the table under "Functional category" section where all the functional categories were listed with their probabilities and odds. For our protein Glucosylceramidase (P04062), "Cell_envelope" in green was considered by ProrFun as the most possible functional category that the protein should belong to.
At the second part, the protein was classified as enzyme or non-enzyme where our protein was correctly predicted as Enzyme.
At the third part,the protein was further classified into 6 different enzyme classes and possible EC numbers were also given. We can see that in that section, ProtFun did not return a significant prediction. No one class from them had a significant higher probability and odds than the others. Even though, Hydrolase (EC 3.-.-.-) with the highest probability 0.272 gave us a good suggestion since we have already known that our protein has EC=3.2.1.45.
At the last part, we can see different Gene Ontology categories with the prediction scores. The Gene Ontology categories listed here seemed different with that from the Gene Ontology. For our protein, ProtFun returned that its most possible Gene Ontology is "Immune_response" which stayed in question. Since there are no further information available about that in ProtFun, it was not easy to judge that prediction.


<figtable id="tab:ss_protfun">

Functional category Prob Odds
Amino_acid_biosynthesis 0.035 1.593
Biosynthesis_of_cofactors 0.182 2.528
Cell_envelope 0.504 8.262
Cellular_processes 0.032 0.438
Central_intermediary_metabolism 0.382 6.063
Biosynthesis_of_cofactors 0.182 2.528
Energy_metabolism 0.067 0.740
Fatty_acid_metabolism 0.027 2.088
Purines_and_pyrimidines 0.538 2.213
Regulatory_functions 0.031 0.191
Replication_and_transcription 0.126 0.471
Translation 0.082 1.863
Transport_and_binding 0.560 1.365
Enzyme/nonenzyme Prob Odds
Enzyme 0.773 2.698
Nonenzyme 0.227 0.318
Enzyme class Prob Odds
Oxidoreductase (EC 1.-.-.-) 0.083 0.399
Transferase (EC 2.-.-.-) 0.228 0.660
Hydrolase (EC 3.-.-.-) 0.272 0.859
Lyase (EC 4.-.-.-) 0.045 0.961
Isomerase (EC 5.-.-.-) 0.011 0.345
Ligase (EC 6.-.-.-) 0.017 0.332
Gene Ontology category Prob Odds
Signal_transducer 0.054 0.251
Receptor 0.027 0.158
Hormone 0.001 0.206
Structural_protein 0.002 0.087
Transporter 0.024 0.222
Ion_channel 0.018 0.307
Voltage-gated_ion_channel 0.004 0.195
Cation_channel 0.012 0.268
Transcription 0.070 0.550
Transcription_regulation 0.030 0.237
Stress_response 0.085 0.962
Immune_response 0.153 1.804
Growth_factor 0.005 0.376
Metal_ion_transport 0.009 0.020

</figtable>


Table 17: GO terms prediction results for P04062 in Protfun2.0.


Pfam

At last, Pfam was used to find pfam family for our protein Glucosylceramidase (P04062). As we can see in the following figure, one domain was found by Pfam: Glycoside hydrolase family 30.
It is a group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. One can find this system on the CAZy web site.
One of the famous enzyme under this group is the mammalian Glucosylceramidase (P04062) which is the protein we are working with. It cleaves the glucosidic bonds of glucosylceramide and synthetic beta-glucosides.Any one of over 50 different mutations in the gene of glucocerebrosidase have been found to affect activity of this hydrolase, producing variants of Gaucher disease, the most prevalent lysosomal storage disease.



References

<references/>