|polar, small, hydrophilic, negatively charged||polar, tiny, hydrophilic, neutral||Both amino acids are polar and hydrophilic. Ser is tiny, Asn therefore is a small amino acid. The biggest difference between these two amino acid is, that Asn is negatively charged and Ser is neutral. But this is not that big difference and therefore we suggest, that this mutation do not delete the structure and function of the protein.|
Visualisation of the Mutation
|picture original aa||picture mutated aa||combined picture|
Subsitution Matrices Values
|PAM 1||Pam 250||BLOSOUM 62|
|value aa||most frequent substitution||rarest substitution||value aa||most frequent substitution||rarest substitution||value aa||most frequent substitution||rarest substitution|
|20||36 (Asp)||0 (Cys, Met)||5||7 (Asp)||2 (Cys, Leu, Phe, Trp)||1||1 (Asp, His, Ser)||-4 (Trp)|
Conservation Analysis with Multiple Alignments
Secondary Structure Mutation Analysis
JPred: CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCEEEEECCCEEEEECCCCCCC... PsiPred: CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEECCCEEEEECCCCCCC...
Comparison with the real structure:
|Substitution||Prediction||Reliability Index||Expected Accuracy|
A detailed list of all possible substitutions can be found [here]
Each entry contains the score at a particular position (row) for an amino acid substitution (column). Substitutions predicted to be intolerant are highlighted in red.
Threshold for intolerance is 0.05.
Amino acid color code: nonpolar, uncharged polar, basic, acidic.
Capital letters indicate amino acids appearing in the alignment, lower case letters result from prediction.