Difference between revisions of "PAH Structure"

From Bioinformatikpedia
(References)
(Domains)
Line 24: Line 24:
 
* the catalytic domain (residues 118-427)
 
* the catalytic domain (residues 118-427)
 
* a C-terminal domain (residues 428-453) responsible for oligomerization of identical monomers
 
* a C-terminal domain (residues 428-453) responsible for oligomerization of identical monomers
  +
  +
=== Regulatory N-terminal domain ===
  +
  +
=== Catalytic domain ===
  +
  +
=== C-terminal oligomerization domain ===
   
 
== References ==
 
== References ==

Revision as of 18:30, 23 August 2011

Phenylalanine hydroxylase is an enzyme, which is necessary for the catalysis of phenylalanine to tyrosine. This catalysis is realized by hydroxylation of the aromatic side chain of phenylalanine. The enzyme needs tetrahydrobiopterin, BH4 a pteridine cofactor, and a non-heme iron for catalysis. In the reaction, molecular oxygen is cleaved. One oxygen atom is incorporated in BH4. The other oxygen atom is incorporated in phenylalanine. The reaction is shown in the following schema.

PAH overallreaction.png

This reaction is the bottleneck in the metabolic pathway, which degrades phenylalanine. Mutations in the gene of PAH can cause the metabolomic disorder phenylketonuria. The structure of phenylalanine hydroxylase is very well reviewed. In this article we want to summarize the results in order to acquired more insight into the effect of mutations of the gene of phenylalanine hydroxylase.

PAH agglomerates in the cell to a homo-tetra-mere. That means, four identical chains of PAH build a complex. A model of this complex is shown below. Pah hydroxylase.png

In this illustration the binding pockets are very obvious. The red Fe atoms in the binding pockets mark their positions.

Reaction

It is assumed, that the reaction takes place in three steps:

  • formation of a Fe(II)-O-O-BH4 bridge
  • heterolytic cleavage of the O-O bond to yield the ferryl oxo hydroxylating intermediate Fe(IV)=O
  • attack on Fe(IV)=O to hydroxylate phenylalanine substrate to tyrosine.

Especially the formation of the Fe(II)-O-O-BH4 bridge is strongly discussed. Therefore most crystallographic experiments focus on the catalytic domain, which contains the BH4 and the Fe-atom.

Domains

Phenylalanine hydroxylase consists of three domains:

  • a regulatory N-terminal domain (residues 1-117)
  • the catalytic domain (residues 118-427)
  • a C-terminal domain (residues 428-453) responsible for oligomerization of identical monomers

Regulatory N-terminal domain

Catalytic domain

C-terminal oligomerization domain

References

Phenylalanine hydroxylase has been the protein of the month (January 2005).