Lab Journal Task 3 Hemo

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Secondary Structure Predictions

  • reprof version 1.0.1
  • psipred version 3.3

The PSSMs used for reprof were selected from the psiblast runs against big_80, SwissProt and PDB with standard parameters (2 iterations and e-value cutoff of 0.002) that were already performed in task2. Reprof was called using

reprof -i <input file>

The Psipred server was run only using the sequences and not with a multiple sequence alignment.

Output unification

DSSP, reprof and psipred all have different output types. In order to compare the results, the following mappings were performed

reprof
H helix
E extended
L loop -> C


DSSP
H = alpha helix -> H
B = residue in isolated beta-bridge -> C
E = extended strand, participates in beta ladder -> E
G = 3-helix (3/10 helix) -> H
I = 5 helix (pi helix) -> C
T = hydrogen bonded turn - C
S = bend -> C


psipred:

H helix
E sheet
C disordered


Disorder Prediction

The IUPred server was used to generate the corresponding predictions. But unfortunately, the server does not show a version number.

Metadisorder was called using the predictprotein command specified in the task description.

Transmembrane Helix Prediction

Polyphobius

For running polyphobius, the [polyphobius.pl] script was used in conjuntion with t


Signal Peptides

signalP server Version 4.1

  • Organism: Eukaryote
  • D-cutoff values: default
  • Input may include TM-helices: yes
  • Restrictions: None

GO Terms