Gaucher Disease: Task 09 - Lab Journal
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Contents
Preparation
1. Choose a structure to work with
In the former tasks, we worked with the reference structure 1OGS, because it has no gaps - but an offset of 39 residues at the N terminus, as all structures for our protein P04062 referenced in UniProt - and has a pretty low resolution of 2.0 Å. However, there are four other structures with a lower resolution (all resolved using the X-ray diffraction method). We compare 1OGS and those four structures for the resolution, coverage and gaps, R-factor, R-free and pH-value at which the structure was resolved in the following table.
<figtable id="structure_choice">
PDB-ID | resolution (Å) | chain | coverage | missing residues | R-factor(obs.) | R-free | pH | temperature (K) |
---|---|---|---|---|---|---|---|---|
1OGS | 2.0 | A-/B | 40-536 (92.7%) | 1-39 | 0.195 | 0.230 | 4.6 | 100 |
2NT0 | 1.79 | A/B/C/D | 40-536 | Gaps | 0.181 | 0.215 | 4.5 | 100 |
PDB-ID | Resolution (Å) | Chain | 40-536 | Gaps | R-factor | R-free | pH | |
PDB-ID | Resolution (Å) | Chain | 40-536 | Gaps | R-factor | R-free | pH | |
PDB-ID | Resolution (Å) | Chain | 40-536 | Gaps | R-factor | R-free | pH |
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