Gaucher Disease: Task 09 - Lab Journal

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This page is under construction.

Preparation

1. Choose a structure to work with

In the former tasks, we worked with the reference structure 1OGS, because it has no gaps - but an offset of 39 residues at the N terminus, as all structures for our protein P04062 referenced in UniProt - and has a pretty low resolution of 2.0 Å. However, there are four other structures with a lower resolution (all resolved using the X-ray diffraction method). We compare 1OGS and those four structures for the resolution, coverage and gaps, R-factor, R-free and pH-value at which the structure was resolved in the following table.

<figtable id="structure_choice">

PDB-ID	Resolution (Å)	Chain	Covered residues	Missing residues	R-Value(obs.)	R-Free	pH	Temperature (K)
2NT0	1.79	A/B/C/D	40-536 (92.7%)	1-39?	0.181	0.215	4.5	100
3GXI	1.84	A/B/C/D	40-536 (92.7%)	1-39?	0.193	0.231	5.5	?
2V3F	1.95	A/B	40-536 (92.7%)	1-39?	0.154	0.196	6.5	100
2V3D	1.96	A/B	40-536 (92.7%)	1-39?	0.157	0.208	6.5	100
1OGS	2.0	A-/B	40-536 (92.7%)	1-39?	0.195	0.230	4.6	100

Comparison of the resolution top five PDB structures according to different other criteria.

</figtable>

Structure 2V3F seems to be a good choice because of the highest pH-value and the lowest R-value and R-free. However, there are some missing residues, 8 in chain A:

REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465 M RES C SSSEQI
REMARK 465 PRO A 29
REMARK 465 THR A 30
REMARK 465 PHE A 31
REMARK 465 LEU A 499
REMARK 465 VAL A 500
REMARK 465 ASP A 501
REMARK 465 THR A 502
REMARK 465 MET A 503
REMARK 465 GLU B -1
REMARK 465 PHE B 0
REMARK 465 ASP B 27
REMARK 465 PRO B 28
REMARK 465 PRO B 29
REMARK 465 THR B 30
REMARK 465 PHE B 31
REMARK 465 PRO B 32
REMARK 465 LEU B 498
REMARK 465 LEU B 499
REMARK 465 VAL B 500
REMARK 465 ASP B 501
REMARK 465 THR B 502
REMARK 465 MET B 503

Sources

PDB R-value and R-free