Difference between revisions of "Fabry:Sequence alignments (sequence searches and multiple alignments)/Journal"

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[[Fabry Disease]] » [[Fabry:Sequence alignments (sequence searches and multiple alignments)|Sequence alignments]] » Journal
Please see [[Fabry:Sequence_alignments_(sequence_searches_and_multiple_alignments):Results | Task 2 Results]] for our results on this topic.
 
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<hr>
Please see also [[Fabry:Sequence_alignments_(sequence_searches_and_multiple_alignments):Scripts | Task 2 Scripts]] for the used scripts.
 
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Please see [[Fabry:Sequence_alignments_(sequence_searches_and_multiple_alignments):Scripts | Task 2 Scripts]] for the used scripts.
   
   
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blastall -p blastp -d /mnt/project/pracstrucfunc12/data/big/big_80 -i P06280.fasta -m 0 -o blastsearch_default.out -v 700 -b 700
 
blastall -p blastp -d /mnt/project/pracstrucfunc12/data/big/big_80 -i P06280.fasta -m 0 -o blastsearch_default.out -v 700 -b 700
perl extract_ids_blast.pl blastsearch_default.out
+
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/extract_ids_blast.pl.html extract_ids_blast.pl]</span> blastsearch_default.out
perl ../download-annotation.pl blastsearch_default_ids.txt
+
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/download-annotation.pl.html download-annotation.pl]</span> blastsearch_default_ids.txt
perl ../compare_GO_terms.pl P06280 blastsearch_default_ids_GOterms.tsv
+
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/compare_GO_terms.pl.html compare_GO_terms.pl]</span> P06280 blastsearch_default_ids_GOterms.tsv
perl parse_blast.pl blastsearch_default.out
+
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/parse_blast.pl.html parse_blast.pl]</span> blastsearch_default.out
  +
  +
R CMD BATCH <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/hist_blast.R.html hist_blast.R]</span>
   
 
=== Psi-Blast ===
 
=== Psi-Blast ===
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The following command was used to run Psi-Blast with AGAL as query sequence against big80. It was run with two and ten iterations configured and an e-value cut-off of 2e-3 and 1e-9, respectively.
 
The following command was used to run Psi-Blast with AGAL as query sequence against big80. It was run with two and ten iterations configured and an e-value cut-off of 2e-3 and 1e-9, respectively.
   
$ bash [https://www.dropbox.com/sh/wsuttl48gkqkuyh/DSm9YUAknM/run_psi_blast.sh.txt run_psi_blast.sh] > run_psi_blast.log
+
$ bash <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/run_psi_blast.sh.html run_psi_blast.sh]</span> &> run_psi_blast.log
   
 
The log file contains the runtimes of the different psi-blast runs:
 
The log file contains the runtimes of the different psi-blast runs:
   
{| class="wikitable" style="text-align: center;"
+
{| style="border-spacing: 0em; text-align: center; margin: 2em 0px;"
 
|-
 
|-
! scope="col"| Iterations
+
! scope="col" style="border-bottom: 2px solid #000; padding: 0px 1em;" | Iterations
! scope="col"| E-value cut-off
+
! scope="col" style="border-bottom: 2px solid #000; padding: 0px 1em; border-left: 2px solid #000;" | E-value cut-off
! scope="col"| Runtime
+
! scope="col" style="border-bottom: 2px solid #000; padding: 0px 2em; border-left: 2px solid #000;" | Runtime
 
|-
 
|-
 
| 2
 
| 2
  +
| style="border-left: 2px solid #000;" | 2e-3
| 2e-3
 
  +
| style="border-left: 2px solid #000;" | 3m0.814s
| 3m4.616s
 
 
|-
 
|-
 
| 2
 
| 2
  +
| style="border-left: 2px solid #000;" | 1e-9
| 1e-9
 
  +
| style="border-left: 2px solid #000;" | 3m9.422s
| 3m9.002s
 
 
|-
 
|-
 
| 10
 
| 10
  +
| style="border-left: 2px solid #000;" | 2e-3
| 2e-3
 
  +
| style="border-left: 2px solid #000;" | 14m29.179s
| 15m20.813s
 
 
|-
 
|-
 
| 10
 
| 10
  +
| style="border-left: 2px solid #000;" | 1e-9
| 1e-9
 
  +
| style="border-left: 2px solid #000;" | 15m39.251s
| 15m37.960s
 
 
|-
 
|-
 
|}
 
|}
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Afterwards, the psi-blast output was parsed to collect the all the information about all the hits of the last iteration, which include the e-value, the sequence identity, the coverage in the longer sequence of the pairwise alignment and the length of the alignment. When there were more than one alignment per hit, we used the first one which was also listed in the short result output.
 
Afterwards, the psi-blast output was parsed to collect the all the information about all the hits of the last iteration, which include the e-value, the sequence identity, the coverage in the longer sequence of the pairwise alignment and the length of the alignment. When there were more than one alignment per hit, we used the first one which was also listed in the short result output.
   
$ for i in psi_results_*.txt; do \
+
$ for i in psi_results_*.txt; do
perl [https://www.dropbox.com/sh/wsuttl48gkqkuyh/j5yPOvLQ-e/parse_psiblast.pl.txt parse_psiblast.pl] "$i" > "${i%.*}.stats" \
+
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/parse_psiblast.pl.html parse_psiblast.pl]</span> "$i" > "${i%.*}.stats"
 
done
 
done
  +
  +
The histograms were generated with the [https://dl.dropbox.com/u/13796643/fabry/msa_scripts/generate_histograms.sh.html generate_histograms.sh] script:
  +
$ bash <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/generate_histograms.sh.html generate_histograms.sh]</span> *.stats
  +
  +
GO term comparison
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$ perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/download-annotation.pl.html download-annotation.pl]</span> ids_psiblast_10its_eVal_1e-9.txt
  +
$ perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/compare_GO_terms.pl.html compare_GO_terms.pl]</span> P06280 ids_psiblast_10its_eVal_1e-9_GOterms.tsv
  +
$ bash <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/hist_psiblast.sh.html hist_psiblast.sh]</span> ids_psiblast_10its_eVal_1e-9_GOterms_comparison.txt
   
 
=== HHblits / HHsearch ===
 
=== HHblits / HHsearch ===
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time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_default.out -E 0.003 -z 700
 
time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_default.out -E 0.003 -z 700
./extract_ids_hhblits.sh hhblits_default.out
+
./<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/extract_ids_hhblits.sh.html extract_ids_hhblits.sh]</span> hhblits_default.out
  +
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/parse_hhblits.pl.html parse_hhblits.pl]</span> hhblits_default.out
perl ../download-annotation.pl hhblits_default_ids.txt
 
  +
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/download-annotation.pl.html download-annotation.pl]</span> hhblits_default.out_cluster_ids_only.tsv
perl ../compare_GO_terms.pl P06280 hhblits_default_ids_GOterms.tsv
 
  +
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/compare_GO_terms.pl.html compare_GO_terms.pl]</span> P06280 hhblits_default.out_cluster_ids_only_GOterms.tsv
perl parse_hhblits.pl hhblits_default.out
 
 
 
 
time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_n8_neu.out -E 0.003 -n 8 -z 800 -b 800
 
time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_n8_neu.out -E 0.003 -n 8 -z 800 -b 800
./extract_ids_hhblits.sh hhblits_n8_neu.out
+
./<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/extract_ids_hhblits.sh.html extract_ids_hhblits.sh]</span> hhblits_n8_neu.out
  +
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/parse_hhblits.pl.html parse_hhblits.pl]</span> hhblits_n8_neu.out
perl ../download-annotation.pl hhblits_n8_neu_ids.txt
 
  +
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/download-annotation.pl.html download-annotation.pl]</span> hhblits_n8_neu.out_cluster_ids_only.tsv
perl ../compare_GO_terms.pl P06280 hhblits_n8_neu_ids_GOterms.tsv
 
  +
perl ../<span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/compare_GO_terms.pl.html compare_GO_terms.pl]</span> P06280 hhblits_n8_neu.out_cluster_ids_only_GOterms.tsv
perl parse_hhblits.pl hhblits_n8_neu.out
 
 
 
R CMD BATCH hist_hhblits.R
+
R CMD BATCH <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/hist_hhblits.R.html hist_hhblits.R]</span>
   
 
== Comparison ==
 
== Comparison ==
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Venn diagrams created with [http://bioinfogp.cnb.csic.es/tools/venny/index.html Oliveros, J.C. (2007) VENNY. An interactive tool for comparing lists with Venn Diagrams.]
 
Venn diagrams created with [http://bioinfogp.cnb.csic.es/tools/venny/index.html Oliveros, J.C. (2007) VENNY. An interactive tool for comparing lists with Venn Diagrams.]
   
>R CMD BATCH all_Evalues.R
+
>R CMD BATCH <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/all_Evalues.R.html all_Evalues.R]</span>
 
   
 
== Multiple sequence alignments ==
 
== Multiple sequence alignments ==
   
  +
The following commands were used in our bash script [https://dl.dropbox.com/u/13796643/fabry/msa_scripts/calculate_msas.sh.html calculate_msas.sh] to generate the multiple sequence alignments. The pictures were obtained by using [http://www.jalview.org/ jalview].
=== Results ===
 
The following commands were used in our bash script [https://www.dropbox.com/sh/wsuttl48gkqkuyh/oCg2gIxjxe/calculate_msas.sh.txt calculate_msas.sh] to generate the multiple sequence alignments. The pictures were obtained by using [http://www.jalview.org/ jalview].
 
   
 
$ clustalw -infile="<filename>.fasta" -outfile="msa/clustalw_<filename>.msa" &
 
$ clustalw -infile="<filename>.fasta" -outfile="msa/clustalw_<filename>.msa" &
Line 89: Line 101:
 
-outfile "msa/3Dcoffee_<filename>.msa" &
 
-outfile "msa/3Dcoffee_<filename>.msa" &
   
  +
We counted the number of gaps and conserved columns with the perl script [https://dl.dropbox.com/u/13796643/fabry/msa_scripts/countGaps.pl.html countGaps.pl]. There is also a small wrapper script - [https://dl.dropbox.com/u/13796643/fabry/msa_scripts/countAllGaps.sh.html countAllGaps.sh] which runs countGaps.pl on all .msa files in a specific folder:
 
We counted the number of gaps and conserved columns with the perl script [https://www.dropbox.com/sh/wsuttl48gkqkuyh/LhTz_1kX9w/countGaps.pl.txt countGaps.pl]. There is also a small wrapper script - [https://www.dropbox.com/sh/wsuttl48gkqkuyh/6WghFAIuB6/countAllGaps.sh.txt countAllGaps.sh] which basically runs countGaps.pl on all .msa files in a specific folder:
 
   
 
#!/bin/bash
 
#!/bin/bash
 
 
 
for file in msa/*.msa; do
 
for file in msa/*.msa; do
perl countGaps.pl "$file" > "${file%.*}.counts"
+
perl <span class="plainlinks">[https://dl.dropbox.com/u/13796643/fabry/msa_scripts/countGaps.pl.html countGaps.pl]</span> "$file" > "${file%.*}.counts"
 
done
 
done
  +
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[[Category: Fabry Disease 2012]]

Latest revision as of 17:06, 9 May 2012

Fabry Disease » Sequence alignments » Journal



Please see Task 2 Scripts for the used scripts.


Sequence searches

Blast

We searched the "big80" database with Blast with the following command:

blastall -p blastp -d /mnt/project/pracstrucfunc12/data/big/big_80 -i P06280.fasta -m 0 -o blastsearch_default.out -v 700 -b 700
perl extract_ids_blast.pl blastsearch_default.out
perl ../download-annotation.pl blastsearch_default_ids.txt
perl ../compare_GO_terms.pl P06280 blastsearch_default_ids_GOterms.tsv
perl parse_blast.pl blastsearch_default.out

R CMD BATCH hist_blast.R

Psi-Blast

The following command was used to run Psi-Blast with AGAL as query sequence against big80. It was run with two and ten iterations configured and an e-value cut-off of 2e-3 and 1e-9, respectively.

$ bash run_psi_blast.sh &> run_psi_blast.log

The log file contains the runtimes of the different psi-blast runs:

Iterations E-value cut-off Runtime
2 2e-3 3m0.814s
2 1e-9 3m9.422s
10 2e-3 14m29.179s
10 1e-9 15m39.251s

Afterwards, the psi-blast output was parsed to collect the all the information about all the hits of the last iteration, which include the e-value, the sequence identity, the coverage in the longer sequence of the pairwise alignment and the length of the alignment. When there were more than one alignment per hit, we used the first one which was also listed in the short result output.

$ for i in psi_results_*.txt; do
    perl parse_psiblast.pl "$i" > "${i%.*}.stats"
  done

The histograms were generated with the generate_histograms.sh script:

$ bash generate_histograms.sh *.stats

GO term comparison

$ perl ../download-annotation.pl ids_psiblast_10its_eVal_1e-9.txt
$ perl ../compare_GO_terms.pl P06280 ids_psiblast_10its_eVal_1e-9_GOterms.tsv
$ bash hist_psiblast.sh ids_psiblast_10its_eVal_1e-9_GOterms_comparison.txt

HHblits / HHsearch

We searched the "big80" database with HHblits using the default settings and also with the maximum number of possible iterations (8) with the following commands:

time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_default.out -E 0.003  -z 700
./extract_ids_hhblits.sh hhblits_default.out
perl parse_hhblits.pl hhblits_default.out
perl ../download-annotation.pl hhblits_default.out_cluster_ids_only.tsv
perl ../compare_GO_terms.pl P06280 hhblits_default.out_cluster_ids_only_GOterms.tsv

time hhblits -i ../P06280.fasta -d /mnt/project/pracstrucfunc12/data/hhblits/uniprot20_current -e 0.003 -o hhblits_n8_neu.out -E 0.003 -n 8 -z 800 -b 800
./extract_ids_hhblits.sh hhblits_n8_neu.out
perl parse_hhblits.pl hhblits_n8_neu.out
perl ../download-annotation.pl hhblits_n8_neu.out_cluster_ids_only.tsv
perl ../compare_GO_terms.pl P06280 hhblits_n8_neu.out_cluster_ids_only_GOterms.tsv

R CMD BATCH hist_hhblits.R

Comparison

Venn diagrams created with Oliveros, J.C. (2007) VENNY. An interactive tool for comparing lists with Venn Diagrams.

  >R CMD BATCH all_Evalues.R

Multiple sequence alignments

The following commands were used in our bash script calculate_msas.sh to generate the multiple sequence alignments. The pictures were obtained by using jalview.

$ clustalw -infile="<filename>.fasta" -outfile="msa/clustalw_<filename>.msa" &

$ muscle -in "<filename>.fasta" -out "msa/muscle_<filename>.msa" &

$ /mnt/opt/T-Coffee/bin/t_coffee -seq "<filename>.fasta" -outfile "msa/tcoffe_<filename>.msa" &

$ /mnt/opt/T-Coffee/bin/t_coffee -seq "<filename>.fasta" -method sap_pair -template_file "<filename>.pdb" \
    -outfile "msa/3Dcoffee_<filename>.msa" &

We counted the number of gaps and conserved columns with the perl script countGaps.pl. There is also a small wrapper script - countAllGaps.sh which runs countGaps.pl on all .msa files in a specific folder:

#!/bin/bash

for file in msa/*.msa; do
	perl countGaps.pl "$file" > "${file%.*}.counts"
done