Fabry:Sequence-based analyses

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Revision as of 18:48, 8 May 2012 by Staniewski (talk | contribs) (GOPET)

The following analyses were performed on the basis of the α-Galactosidase A sequence.

Secondary structure

Disorder

Transmembrane helices

Signal peptides

GO terms

GOPET

Searching the GOPET annotation tool with the AGAL_HUMAN sequence revealed 5 GOIds, which are displayed in <xr id="tab:GOPET"/>. On a first glance, since we already know the name and function of the protein, it is a bit surprising, that alpha-galactosidase activity is only the third entry with 96% confidence. In our already carried out information gathering we learned that α-galactosidase A is a hydrolase thus the first three entries were not surprising. Considering that our enzyme mainly is a glycosidase, the both entries on top of the list make perfekt sense.
Again a bit surprising was the last entry. α-N-Acetylgalactosaminidase is actually used for enzyme replacement therapy, which we mention on our main page. The structure of both enzymes is similar to each other, but this still does not explain the association of this GO term to the AGAL protein.

<figtable id="tab:GOPET"> The results of the GOPET search

Result for GOPET search
GOid Aspect Confidence GO term
GO:0016798 Molecular Function Ontology (F) 98% hydrolase activity acting on glycosyl bonds
GO:0004553 Molecular Function Ontology (F) 98% hydrolase activity hydrolyzing O-glycosyl compounds
GO:0016787 Molecular Function Ontology (F) 97% hydrolase activity
GO:0004557 Molecular Function Ontology (F) 96% alpha-galactosidase activity
GO:0008456 Molecular Function Ontology (F) 89% alpha-N-acetylgalactosaminidase activity

</figtable>

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