Difference between revisions of "Canavan Task 3 - Sequence-based predictions"
(→Q9YDF8) |
(→Q9YDF8) |
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Yet, both annotations taken together represent the identified TMH found with Phobius (if one takes into account a shift of +12). |
Yet, both annotations taken together represent the identified TMH found with Phobius (if one takes into account a shift of +12). |
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+ | [[File:1orq_seq_shift.jpg]] |
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− | Sequence alignment between 1orq(C) and UniProt seq Q9YDF8 (KVAP_AERPE): |
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− | Q9YDF8 (KVAP_AERPE): Voltage-gated potassium channel OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) GN=APE_0955 PE=1 SV=1 |
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− | |||
− | 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 |
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− | ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+----- |
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− | UniProt: MSVERWVFPGCSVMARFRRGLSDLGGRVRNIGDVMEHPLVELGVSYAALLSVIVVVVEYTMQLSGEYLVRLYLVDLILVIILWADYAYRAYKSGDPAGYVKKTLYEIPALVPAGLLALIEGHLAGLGLFRLVRLLRFLRILLIISRGSKFLSAIADAADKIRFYHLFGAVMLTVLYGAFAIYIVEYPDPNSSIKSVFDALWWAVVTATTVGYGDVVPATPIGKVIGIAVMLTGISALTLLIGTVSNMFQKILVGEPEPSCSPAKLAEMVSSMSEEEFEEFVRTLKNLRRLENSMK |
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− | ..........................................................X............................................................................................................................................................................................................................................ |
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− | PDB seq: ------------------------------IGDVMEHPLVELGVSYAALLSVIVVVVECTMQLSGEYLVRLYLVDLILVIILWADYAYRAYKSGDPAGYVKKTLYEIPALVPAGLLALIEGHLAGLGLFRLVRLLRFLRILLIISRGSKFLSAIADAADKIRFYHLFGAVMLTVLYGAFAIYIVEYPDPNSSIKSVFDALWWAVVTATTVGYGDVVPATPIGKVIGIAVMLTGISALTLLIGTVSNMFQKILV------------------------------------------ |
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− | --+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ |
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− | 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 |
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== Signal Peptide Prediction == |
== Signal Peptide Prediction == |
Revision as of 14:31, 14 May 2012
Oh, I would sing of mackerel skies, And why the sea is wet, Of jelly-fish and conger-eels, And things that I forget. (taken from "The Cumberbunce" by Paul West)
Contents
Protocol
Commands, Source Code and other methodocial issues are kept in the protocoll.
Secondary Structure Prediction
Information on Proteins
//TODO: picsPrediction of disordered regions
Transmembrane Helix Prediction
Information on Proteins
TM prediction of our Protein yielded the expected prediction of only cytoplasmic residues.
P35462
<figure id="CD_tm_3pbl">
</figure>
Polyphobius found 7 TMH for P35462. This is in accordance with the annotation for transmembrane regions in UniProt. There is only one structure listed for P35462: 3pbl. The annotation for TMH for this structure found in PDBTM and OPM also agrees with the Polyphobius result.
In <xr id="CD_tm_3pbl"/> the length distribution for the predicted and annotated TMH is depicted. One can see that PDBTM in general finds shorter TMH, whereas Polyphobius and OPM find longer helices.
<figtable id="table_2D57">
UniProt | Polyphobius | PDBTM | OPM |
33 – 55 | 35-52 | 34-52 | |
66 – 88 | 68-84 | 67-91 | |
105 – 126 | 109-123 | 101-126 | |
150 – 170 | 152-166 | 150-170 | |
188 – 212 | 191-206 | 187-209 | |
330 – 351 | 334-347 | 330-351 | |
367 – 388 | 368-382 | 363-386 |
</figtable>
P47863
<figure id="CD_tm_2D57">
</figure>
Polyphobius found 6 TMH for P47863. This is in accordance with the annotation for transmembrane regions in UniProt. There are three structures listed for P47863:
- 2D57 X-ray 3.20 A
- 2ZZ9 X-ray 2.80 A
- 3IYZ electron microscopy 10.00 A
Since 2ZZ9 is a mutant, we decided use 2D57 for comparison with the Phobius Output. Interestingly, OPM lists 8 TMH for P47863, whereas PDBTM agrees with the UniProt annotation and the Polyphobius output. The two additional TMH found by OPM are rather short (<10 AA) and therefore might be neglected. In <xr id="table_2D57"/> the AA positions for the predicted and annotated TMH are given.
<figtable id="table_2D57">
UniProt | Polyphobius | PDBTM | OPM |
37 – 57 | 39-55 | 34-56 | |
65 – 85 | 72-89 | 70-88 | |
95-106(loop) | 98-107 | ||
116 – 136 | 116-133 | 112-136 | |
156 – 176 | 158-177 | 156-178 | |
185 – 205 | 188-205 | 189-203 | |
209-222(loop) | 214-223 | ||
232 – 252 | 231-248 | 231-252 |
</figtable>
For the remaining TMH, all three methods find TMH of about the same length.
In <xr id="CD_tm_2D57"/> the length distribution for the predicted and annotated TMH is depicted.
Q9YDF8
<figure id="CD_tm_Q9YDF8">
</figure>
For Q9YDF8, Polyphobius did not find any homologues with the blast search. Therefore, no homolgy information could be used for the TMH prediction.
In UniProt one can find the annotation for 6 TM regions and 2 intramembrane regions for this protein and lists four structures:
- 1ORQ X-ray 3.20 A 31-253
- 1ORS X-ray 1.90 A 33-160
- 2A0L X-ray 3.90 A 20-259
- 2KYH NMR - 19-160
Since in 1ORS, only residues 33-160 have been crystalized, we decided to use 1ORQ for comparison with the Polyphobius output.
Polyphobius finds 7 TMH, which correlates with the UniProt annotation:
UniProt | Polyphobius |
39 – 63 | 42-60 |
68 – 92 | 68-88 |
109 – 125 | 108-129 |
129 – 145 | 137-157 |
160 – 184 | 163-184 |
196 – 208(intramembrane) | 196-213 |
222 – 253 | 224-244 |
However, OPM and PDBTM list very diverse results. There is only a consensus on TMH 5 and 7. In <xr id="CD_tm_Q9YDF8"/> the length distribution for the TMH prediction is presented.
When comparing the annotation of OPM for the two structures 1orq and 1ors, one can find tremendous differences:
- 1ors: C - Tilt: 19° - Segments: 1(25-46), 2(55-78), 3(86-97), 4(100-107), 5(117-148)
- 1orq: C - Tilt: 31° - Segments: 1(153-172), 2(183-195), 3(207-225)
Yet, both annotations taken together represent the identified TMH found with Phobius (if one takes into account a shift of +12).
Signal Peptide Prediction
Information on Proteins
GO terms and Pfam
Pfam
AstE_AspA family: Succinylglutamate desuccinylase / Aspartoacylase family