Difference between revisions of "BCKDHA"

From Bioinformatikpedia
(Created page with "==Summary== The gene BCKDHA encodes the E1 subunit of the human branched-chain alpha-keto acid dehydrogenase complex (BCKDC). It locates on chromosome 19 and has 34,217 bp. ==…")
 
m (Disease causing mutations)
 
(22 intermediate revisions by the same user not shown)
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BCKDHA is the α subunit of [[Maple_Syrup_Urine_Disease#Branched-chain_.CE.B1-keto_acids_dehydrogenase_complex|branched-chain α-keto acid dehydrogenase complex]]. The gene which encodes BCKDHA locates on chromosome 19.
==Summary==
 
The gene BCKDHA encodes the E1 subunit of the human branched-chain alpha-keto acid dehydrogenase complex (BCKDC).
 
It locates on chromosome 19 and has 34,217 bp.
 
   
 
==Reference sequence==
 
==Reference sequence==
  +
{{{>gi|260593636|ref|NG_013004.1| Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), RefSeqGene on chromosome 19
 
  +
* [[#Genomic_sequence|Genomic sequence]]
  +
* [[#mRNA_transcript|mRNA transcript]]
  +
* [[#Protein|Protein sequence]]
  +
  +
===Genomic sequence===
  +
<nowiki>>gi|260593636|ref|NG_013004.1| Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), RefSeqGene on chromosome 19
 
GGCAGCTGGTAGGGAGGAGCTGGGGGGGTGATTTTGACAAACTGGGCACCACTCAGAGGTTCAGCAGGGC
 
GGCAGCTGGTAGGGAGGAGCTGGGGGGGTGATTTTGACAAACTGGGCACCACTCAGAGGTTCAGCAGGGC
 
CCAATTACCAGGCAGCCCAATCTTCGGCCTCAGGATCACTTCGAGTGTGGCCTTGTTGAAAATCTCTTTG
 
CCAATTACCAGGCAGCCCAATCTTCGGCCTCAGGATCACTTCGAGTGTGGCCTTGTTGAAAATCTCTTTG
Line 493: Line 497:
 
AGCTACAAGGGAGCCACAGGGGAGCCACGGAGGCCATTGGGGTGTGGGGATGGGCCTCCAGAGAGGGCCC
 
AGCTACAAGGGAGCCACAGGGGAGCCACGGAGGCCATTGGGGTGTGGGGATGGGCCTCCAGAGAGGGCCC
 
AGCCAGGCCCCAGGCAGACAGCTTCACAATCTCCCATAGTCCCCGCCAACCCCCATAACAGATTCTCTCG
 
AGCCAGGCCCCAGGCAGACAGCTTCACAATCTCCCATAGTCCCCGCCAACCCCCATAACAGATTCTCTCG
GTCCCAGATCCTAAACAGCTCCCCTGACCCAGGGACCTCGTTGGTCCTTATTCCTAG}}}
+
GTCCCAGATCCTAAACAGCTCCCCTGACCCAGGGACCTCGTTGGTCCTTATTCCTAG</nowiki>
  +
  +
===mRNA transcript===
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<nowiki>>gi|258645170|ref|NM_000709.3| Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA
  +
CTACGTGAGTGCCGGACCGCTGAGTGGTTGTTAGCCAAGATGGCGGTAGCGATCGCTGCAGCGAGGGTCT
  +
GGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGCTGCTGCGGCAGCCTGGGGCTCGGGGACTGGC
  +
TAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATCTCTGGATGACAAGCCCCAGTTCCCAGGGGCC
  +
TCGGCGGAGTTTATAGATAAGTTGGAATTCATCCAGCCCAACGTCATCTCTGGAATCCCCATCTACCGCG
  +
TCATGGACCGGCAAGGCCAGATCATCAACCCCAGCGAGGACCCCCACCTGCCGAAGGAGAAGGTGCTGAA
  +
GCTCTACAAGAGCATGACACTGCTTAACACCATGGACCGCATCCTCTATGAGTCTCAGCGGCAGGGCCGG
  +
ATCTCCTTCTACATGACCAACTATGGTGAGGAGGGCACGCACGTGGGGAGTGCCGCCGCCCTGGACAACA
  +
CGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTGATGTATCGGGACTACCCCCTGGAACTATT
  +
CATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAAGGGGCGCCAGATGCCTGTCCACTACGGCTGC
  +
AAGGAACGCCACTTCGTCACTATCTCCTCTCCACTGGCCACGCAGATCCCTCAGGCGGTGGGGGCGGCGT
  +
ACGCAGCCAAGCGGGCCAATGCCAACAGGGTCGTCATCTGTTACTTCGGCGAGGGGGCAGCCAGTGAGGG
  +
GGACGCCCATGCCGGCTTCAACTTCGCTGCCACACTTGAGTGCCCCATCATCTTCTTCTGCCGGAACAAT
  +
GGCTACGCCATCTCCACGCCCACCTCTGAGCAGTATCGCGGCGATGGCATTGCAGCACGAGGCCCCGGGT
  +
ATGGCATCATGTCAATCCGCGTGGATGGTAATGATGTGTTTGCCGTATACAACGCCACAAAGGAGGCCCG
  +
ACGGCGGGCTGTGGCAGAGAACCAGCCCTTCCTCATCGAGGCCATGACCTACAGGATCGGGCACCACAGC
  +
ACCAGTGACGACAGTTCAGCGTACCGCTCGGTGGATGAGGTCAATTACTGGGATAAACAGGACCACCCCA
  +
TCTCCCGGCTGCGGCACTATCTGCTGAGCCAAGGCTGGTGGGATGAGGAGCAGGAGAAGGCCTGGAGGAA
  +
GCAGTCCCGCAGGAAGGTGATGGAGGCCTTTGAGCAGGCCGAGCGGAAGCCCAAACCCAACCCCAACCTA
  +
CTCTTCTCAGACGTGTATCAGGAGATGCCCGCCCAGCTCCGCAAGCAGCAGGAGTCTCTGGCCCGCCACC
  +
TGCAGACCTACGGGGAGCACTACCCACTGGATCACTTCGATAAGTGAGACCTGCTCAGCCCACCCCCACC
  +
CATCCTCAGCTACCCCGAGAGGTAGCCCCACTCTAAGGGGAGCAGGGGGACCTGACAGCACACCACTGTC
  +
TTCCCCAGTCAGCTCCCTCTAAAATACTCAGCGGCCAGGGCGGCTGCCACTCTTCACCCCTGCTCCTCCC
  +
GGCTGTTACATTGTCAGGGGACAGCATCTGCAGCAGTTGCTGAGGCTCCGTCAGCCCCCTCTTCACCTGT
  +
TGTTACAGTGCCTTCTCCCAGGGGCTGGGTGAGGGCACATTCAGGACTAGAAGCCCCTCTGGGCATGGGG
  +
TGGACATGGCAGGTCAGCCTGTGGAACTTGCGCAGGTGCGAGTGGCCAGCAGAGGTCACGAATAAACTGC
  +
ATCTCTGCGCCTGGCTCTCTACCAAAAAAAAAAAAAAAAAA</nowiki>
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  +
===Protein===
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<nowiki>>gi|11386135|ref|NP_000700.1| 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor [Homo sapiens]
  +
MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQP
  +
NVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGT
  +
HVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLA
  +
TQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYR
  +
GDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDE
  +
VNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVYQEMPAQL
  +
RKQQESLARHLQTYGEHYPLDHFDK</nowiki>
  +
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==Neutral mutations==
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==Disease causing mutations==
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Several disease causing mutations are reported in [http://www.omim.org/entry/608348 OMIM]. Following table summarize the reported allelic variations:
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{| class="wikitable" border="1" style="width:500px"
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|+ Reported allelic variants in OMIM
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|-
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! ID
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! Variant type
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! Mutation detail
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! Cause
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! Severity
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|-
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| 1 || substitution || TYR393ASN || [[Maple Syrup Urine Disease|MSUD]] type 1A || classic
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|-
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| 2 || deletion || 8-BP DEL, 887-894 || MSUD type 1A || classic
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|-
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| 3 || substitution || GLY245ARG || MSUD type 1A || intermediate
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|-
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| 4 || substitution || PHE364CYS || MSUD type 1A || intermediate
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|-
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| 5 || substitution || ARG220TRP || MSUD type 1A || classic
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|-
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| 6 || substitution || GLY204SER || MSUD type 1A || classic
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|-
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| 7 || substitution || THR265ARG || MSUD type 1A || classic
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|-
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| 8 || substitution || CYS219TRP || MSUD type 1A || classic
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|-
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| 9 || deletion || 1-BP DEL, 117C || MSUD type 1A || classic
  +
|}
  +
  +
==References==
  +
[http://www.ncbi.nlm.nih.gov/gene/593 branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA)]
  +
  +
[http://www.ncbi.nlm.nih.gov/nuccore/260593636?report=graph RefSeqGene of branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA)]
  +
  +
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000709.3 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA]
  +
  +
[http://www.ncbi.nlm.nih.gov/nuccore/NM_001164783.1 Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 2, mRNA]
  +
  +
[http://www.ncbi.nlm.nih.gov/protein/NP_000700.1 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor (Homo sapiens)]
  +
  +
[http://www.omim.org/ Online Mendelian Inheritance in Man]

Latest revision as of 18:04, 28 April 2013

BCKDHA is the α subunit of branched-chain α-keto acid dehydrogenase complex. The gene which encodes BCKDHA locates on chromosome 19.

Reference sequence

Genomic sequence

>gi|260593636|ref|NG_013004.1| Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), RefSeqGene on chromosome 19
GGCAGCTGGTAGGGAGGAGCTGGGGGGGTGATTTTGACAAACTGGGCACCACTCAGAGGTTCAGCAGGGC
CCAATTACCAGGCAGCCCAATCTTCGGCCTCAGGATCACTTCGAGTGTGGCCTTGTTGAAAATCTCTTTG
CTGACCTTCACCTCGGCCGGCCCGTACACACCCGCCAGGACAGAGGTGTCACCTGAGGAGACAGCAGGGA
GGGCCTCACTCACAGCTTCCCTGCTCCTGGGGCCCCATTTGTGGCACTGAGCCTGACGGCCAGGGCTCCC
CACGTCACCCCCGCCATTTGTTGGTACTGATTCTTTGAGCACTCACTTCATTTCTCTCATTTGTAAAATG
GGGAAAATAGCAGTCCCTCCCCGCTAGCGTCTTGTGAGGATCAAAGGACACAATTCACAGAAAACACTTA
GAACACTGCGGGGCTCACGCTGAATGTGCAACAGACTTTAGCTGTATTAGTAACATTCGTCCATTCAGGC
ATTCAGGCAGGGTCTCACTCTCATTGCCCAGGCTGGAGTGCAGTGGTGCCATCACGGCTCATTGCAGCCT
TGACCTTCTGGGCTCAAGTGATCCTCCCGCCTCAGCCTCCCAAGTAGCTGGAACTACAGGCATGTGCCAC
CATGCCTGACTAATTTTTATATTTTTAGTAGAGAGGGGATTTTGCCATGTTGCTCAGGCGGGTTTCGAAC
TCCTGGGCAACTGTAATCCCAAGGTGCTAGGATTACAGGCGTGAGCTACCACGCCTGGCCCTGATATTAG
TAATTTTTTTTTTTTTAATGAGATGGAGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTAATCTC
GGCTCACCGCAACCTCTGCCTCCCGGGTTCAAGCAGTTCTCCTGCCTCAGCCTACTGAGTAGCTGGGATT
ACAGGCGCGCGCCACCACACCTGGCTAATTTTTTTATTTTTAGTAGAGACTGGGTTTCACCATGTTGGTC
AGGCTGGTCTCGAACTTCCTGACCTCATGATCTGCCCGCCTCCCAAAGCGCTGGGATTACAGGCGTGAGC
CACTGTGCCCGGCTGGTATTAGTAACTTTTAACAGGAAGTTCTTCCTGAAAGCTAACCATCCTCCCTCTT
ACTGCAGCTGCTCTCAACTTCTCTTTCCAAAGCTTCCCCAGCCCAGCTCTTCCCTTAGGCACTGGCCCCC
AAATTTTGCATCAGTCTGGACACCTGAGAAAGGGATAATATCTGGAATGAATCCTGATTCCTTATTTCCC
CAGAAGCTATGAACGGGGTACAGGAGGTAAAACCTAAGAAATAAACAGCCTGGCTTCTAGTACTGTCTGC
TGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCACCTGAGGTCAGGAGTTTGAGACCATCCTAG
TCAACATGGTGAAACCCTATCTCTACTAAAGATACAAAAATTAGCTGGGCATAGTGGTGGGTGCCTGTAA
TCTCAGCTACTTGGGAGGCCGACGCAGGAGAATTGCTGGAACCGGGGAGGCGGAGGTTGCAGTGAGCCGA
GATCACACCATTGCACTCCAGCCTGGACAATAACAGCAAGGCTTAGTCTCAATAAAAAAAAAAAAATACA
TTTTAGGCCAGGCATGGTGGCTCACACCTGTAATCCCAGCACTATGGAGGCCCAGGTGGGAGGACTGCTT
GAGGCCAGGAGTTTGAGACCAGCCTGAGCAGCATAGGAAGACTCCTGTCTCTATTTTCTTTTTTGGGACA
GAGTTTCCCTCTGTCACCCAGGAGGCTGGAGTGCAGTGGCGCAATCTCAGTTCACTGCAACCTCCGCCTC
CCAGGCTCAAGTGATTCTTGTACCTCAACCTCCAGAGTAGCTGAGATTACAGGTGCGTGCTACCACATCC
GACTAATTTTTGTATTTTTAGTAGAGATGGGTTTTCACCATGTTGGCCAATCTGGTCTCAGAACTCCTGA
CCTCAACCAAGCGAGGTGGCTCACGCCTGTAATCCCAGCACTGTGGGAGGCTGAGGCAGGTGGATCACAG
GGTCAGGAGTTCAAGATCAGCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAACCACAAAAATTAG
CCGGGCGCGGTGGCACGCACCTGTGGTCCCAGCTACTCAGGAGGCTGAGGCAGAAGAATTGCTTGAACCC
GGGAGGTGGAGGGTGCAGTGAGCCACGATCACTTCACTGCACTCCAGCCTGGGCGACAGGGCGAGACTCC
ATCTCAAAAAAAAAAAAAGAAAAAAAAAAAAAGAACTCCTGACCTCAGAGGCCTCCCAAAGTGCTGGGAT
TACCGCTGTGAGCAACTGCACCCGGCCTCTTCTTAATTCTTACGTTCGAAGAAACTTTAGGTATCTCAAT
TCAGCATAAGCGCTAAGCCTGAGGGAGGGCCTGGGAAGGTCCTTGGTTAAGGCCGTATTTCCTGGGAAGC
CCAATTTGCCCTTACACGGAAGGTATGCTTAGTTCCAGGAGAAACTGCCCTGAGGACTGGAGAAGTTACC
AAAACAGAACTTGCCTCTGGCTCCAGATGAACTGCCTGTGTTTTGCCCGAGCACAAGAGTCTCTCCCCTC
TCACCTTCTTGAAGACGTGGCTCCAGCAATTCTCTCCCCTGTTGACCCCACAACAATCTACAACTCCTGC
CCCACTTATCTGATCCTCTTACAGCAGAACTCCTGAACAGCTGCCTCTTCTTCCTTTTCTGTGACCCCAC
CACTCCATTGCGGCAGCTCAACATCGTCACCACTGACCTCCAAATGATCAACCCTCCCATGACCCGGCTG
TCAGTAAGGCTGAAACAGCTGGTCAGCCCTCACATCTCAGACATGCTCTTTTCTTGGCCCCAGGTCACCA
CCATCTCCTGGTTTCCCCCTAACTCTCTGGGCGTTCCTTCTGAGTCTCCTTTGCTAGCTTGTCACATGTC
TCTGACTGCTCAAGTTGGAAGGCCTAGGGCTTGGTCCTGGGACCTCTTCTCTTTTCCATCTACACTCACT
TCGTTCAGGCAATAGCCTGAAATACCATCTATATAGCCAGAGAGTTCCAAATTGCCTATGTCCAGCTTGG
GTCTCTCTTCTGATTTCCAGACTTGTACAGATCTGGGCAGAAACCTTAGTTATGCCTGAATCTTTTTCCT
CGCACTCAAACCAAACTATACGTAAGTTTTGTCCGTATTACTTTCAAACTACATCCAGAATCTCATCACC
TCCACTACTCCTGCTCTGGCTGGAGCCACCATCACCTCCCTGTTTTCCCCCATACTCCCCACAATCAACA
CAGCAGCCAAATTTTGTTAAAATGTATTGGGGCTGGGCGTGGTGGCTCATACCTGTATGAGCACTTTGGG
AGGAGGATTGCTTGAGCCCAGGAGTTCAAGACCAACCTGGGTGACATCGTGAGACGCTGTCTCAATTTTT
TTTTTTTTTGAGACAGAGTCTTGCTTTGTCGCTCACGCTGGAGTACAATGGCACGATCTCGGCCCACTGC
AACTTCTGCCTCCCAGGTTCAAGTGATTGTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCGCCC
GCCACCATGCCTGGCTAGTTTTTGTATTTTTAGTAGAGACAGGGTTTCGCCATGTTGGCCAGGCTGGTCT
CGAACTCCTGACCTCAGGTGAGCCACAGGTCTTGGCCTCCCAAAGTGTGGGGATTACAGGCGTGAGCCAC
CGCCCCCGGCCTCCCGTCTCAAACGTTAAAGAGAAATATTCTTGGAGTTAATCTACTTCTGAGAGTTATT
TAGCTAGGAAAAGGGGATATTTGCTCTATAGGTAAGACTTGCCCTAATCTTTTTTTTTTTTTTTTTTTTG
ACTTGCCCTAATCTTAGGGATATTTACTTGTGTGGAGAGATGCAGTGAGGTAGCGGTGGGGATGTATTTC
TCAACCCAGCGCAATGACATTATCTTCAATTTGAGGAGTATTTACGTGGGTGAAAGATCCGCCACAGTCC
ACAGTATGCAAATAGACAGGTTTGCTTCAGTCCGGGATGCAAGGACCTAGAGGGTGTGTGTGTGGGGGGT
GGGGGTGGGAGGGTGGAGGAGGAATCGAGTTAAGGGGGATATTTACCCTGGCGAGGTGATTTTCCCTATT
CTGAAATATCAATTCTTGGGCGGACAGTCTGCCCCTCCTCTCATTCTCAGAGATAGTTACAAAGATGGAA
CTATCTTCTCCTTTCTAGTCGTGAGGGAGAGATGTGTGTATCCCTGCGTATTTAGTAGTATTCAATCGGA
CAAGAAATCATGCATCAACGCAGGAGTGACATTTACCAGCTGGTATCGTGAGGAGGTGGGCACTCGGTTG
CACAGTACACGGTAGTCCCTCTCCAAAAGAAAGACCTGGGTGAAGTTCTTACCTTGCAGGAAGGAAGCAG
AGCCATCTGGCCGCGACAGCAGGTTCTGTTCGCAGGCAAAGTGCCGGAGGCTGCAGCCAGGACCCCGAGG
GCTGGACCCTGTTCCATTTTCAGCACGGATTTTGGCGTCAGTATGCGTCTCCTCCTCCATCGCGCCGAGC
CCACGTGCGGCTGCAGTTGTCACTTCCGCCTGGCAGCGCGCGCCGCAAACAGGCTGCGTAACCAGTGCGC
ACGCGCCGGGCTGGGCCATCCTCCTGGGCGTCCGCACGTGGCTTAGCGTTTACCAACAATCTGGCTCCGC
CCAGTCCCACCTCCTAACTCTGGCAACGCCATTGGACATTTGGGAACAGAAGGCAGGCCTTGGCATTGAT
CCGCTTTACTCTGGGCCCCCGGGCCCTAAATCCCTGAGGCCCTGGTCAGGTTGCCCTCTTCTTTGCCTGT
TACAGGAGTAACTACAATGAGGCGATTGGAGAAAATTTTGGGTAGATAGATTTTTTTTTCTCCAAATCTC
TGTGCATTCTATCTACCACAGAACGGGAAGAAGATGGTTTTCTTGTCAGATGGTGCTGGAGTTTGCGTCA
GGCACATAAAGAGGCAGCCCCGAATCTAGCCTACGTGAGTGCCGGACCGCTGAGTGGTTGTTAGCCAAGA
TGGCGGTAGCGATCGCTGCAGCGAGGGTCTGGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGCT
GCTGCGGCAGCCTGGGGCTCGGGGACTGGCTAGATCTGTGAGTACCTGGGCCCCAGGCGGTTTTCCCAAA
GGGGATTAGGGATGTAAAGGCTATCTTCAGAGTGTGGGGTCCCTGAAGGATATGAAGGAAGGGCTGTCAC
AAAAGGGAAAAAGAGTGGGAGACTCCTTGGAGAAGACACCGAAGGGAAGATCTGCCACTTCCTTAGGAGA
CGAAAAGAACTTCCACTTCTTTAGGAGAGGAAAAAACCCGTTTGCAACTTCTTTGTGGGCCCTGAGGGAG
ACGACTTGAAGAGAGAGATGGGGGTTCCTTATTAGCTGCCACTGGTATTCAGGGGAGGCTCCTTGGAGGA
ACAGGGGAAGACCGCAAGACCTTCTCCCTCAGGGTTTGGAGAATGAAACTGACTTTGTAAGGGAGGGACT
TTCTTCTGGGCAAAGATCCCTCTTCAAGAAGAAGAAAGAAGAGAACAGGTCCCTGTCACAGGCTGCATAG
GGCCTTGTATTTCTTTGCTCCTTTGCCACAATTCTGATTTTAAAATGAATTGTGTAAAAAATTTGTTTGA
TTCCTGTGTCTCTTGATCGAATATCAAGACAGAGAATTCGTGTGTTCTGGACACTGTACAGAGGAGACGA
TTAATATTTGTTGAATTAATGAGGGGAAAAAAGGAGGGAGAGAGGTACAGACAGGGCACTTTGCCCACAT
TTCTTAGAAACACTTGAGAAGGCTTTGTTTCTTGTCAAAAGGGTCACAATCCCTTCAGGTGGATGCTGCT
AAGGGCAAGATTACACTGCAAAAAACCTAGGTGGAAGCCTATCTTTAGACGGGGAGAGGCAATGGGTTGC
AGAAGAGCTTTGAACTACAGTTGACAGATAGGAGAGTAGTGGTTACATGGGTGGATGCATGTCCTTGCTC
TGCCGCTTTCCACTTGTGTAGCCTTGGCCAACGGACTGCACTTCTCTGGATCTATGGAGCTAATTTTCCT
CTCTAAAATATTAACAGTGGCCACCTCATAGGGCTGTGAAAAATCAATATGTAAAATGCTAAGGACTGGA
CCTGGCACATCCTAAGGGCAATAGGAATGTTGATAGTGAGGGTGGAGGGAAGGGACAGAAACTAACCTTG
GAGATGGGGAGAAGAAAGGGGTGACCTTGGGAAGGCCTGGATTGGTGAGGCCCTGGGGAAAAAAATCTGA
AAAGATATTGTGAGGTTGAGTCTGATCCTCTGATCCCCAATCCATAAGGAGGGGAAGATGGAATCTGTAG
AAAGAAGCTCTGGGTAGGGGAACAAACGGGAGGAGGGTGAAGAAGATGGGCCAGGAGTAGGACGTAGTAG
GTATCTAGGAACGGAGTCACTTGAAACAGGTAAATACAGAAGGGCTGCAGGAGCAAGGTGTGGGTAGGGC
AGTCTCTTGCCTTTGGGCTGGAGACCTTTTCACTTTTTTTTTTTTTTTTTTTTTTGAGCAGTTCATATCC
TCCACAGCTGATCTCAGTCCCAGGGGCCCATTGGCTGGGCAAGTGGGGCAGGAGGTGGGCAGGACTGGGG
CAGGCTAGGGAAAGGTCATTTATGGCTGATTAAATCTTTCTCTCCTGTCTCAATCTCTTGATTACCTTTC
CTCTTGCTTCCCCTACTTCATTTTTGGTGGGGAGTGGGGAGGGAAGAAGTCCCTACTTTTTATTTATATA
TATTTAAATTTGTATTATCTTAAGAGTTATTGTTTTTTCCCCTTTATTTCTCCCACCTGTTCTTTCTCTC
TTCTTCTTTCTTTCCTCTCCTTCTCCTCTCCTCTCCTCCCCTCTCCTTTTTCTTTTCTTTTCTTTTTCCC
TTTCCTTTCCTTTCCTTTTCTCTTCTCTTTTCTCTCTCTCTTTCTTTCTGTACCTCTCTTTCCTTTTTCT
TTTTTTTTCTTTTTTGAGACAGAGTTTCACTCTCTCGCCTAGGCTGGAGTGCAGCAGCATGATCTTGACT
TACTGCAATCTCTGCCTGCCGAGTTCAAGCAATTCTCCTGCCTCAGCCTCCCTAGTAGCTGGGATTACAG
GCATGCACTATCACGCCTGGCTAATTTTTGTATTTTTGGTAGGGACGAGGTTTCACCATGTTGTCCAGGC
TGGTCTCGAACTTCTGGCCTCAAGTTCCGCCTGTCTTGGCCTCCAAAAGCATGAGCCACTGCGCCTAGCC
TTTTCTTTTTTTGAGACAAGGTCTCACTCTGTCACCCAGGCTGGAGTGAGTGCAGTGGTGTGATCATGGC
TCACTGCAGCCTCAACCTCCTGGGCTCAAGCAATCCCCCCACCTCCACCTCCTAAGTAGCTGGGACCACA
GGCACGTGCTACTACGCCTGGCTAATTTTTAATTTTTTTTTTTTTTTTTGAGACGGAGTCTTGCTCTGTC
ACCCAAGCTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAACCTCCACTTCCCAGGTTCAAGTGATTCT
CCTGCCTCAGCCTCCCAAATAGCTGGGACTACTGGCGTGCGCCACCATACCCAGTTAATTTTTGTATTTT
TAGTAGAGATGGGGTTTCACCATGTTGACCAGGCTGGTCTTGAACTCCTGATCTAAGGTGATCTGCCCGC
CTCGGCCTCCCAAAGTGCTGGGATTACAGGCATAAGCCACTGTGCCTGGCTGCGCCTGGCTAATTTTTAA
ATCTTTGTAGAGATGGGGTTTTGCCATGTTTTCCAGGCTGGTCTTGAACTCCTGGACTCAAGCGATCTTC
CTGCCTCAGCCTCCCAAAGTGCAGGATGACAAGCGTGAGCCACCTTGCCTGGCCATATTATTAAATTATC
ATGAGTTTAAGTTTAAGTAGCCACAAGTGGGCTGGGTGCGGTGGCTCATGCCTGTAATCCCAGCATTTTG
AGAGGCCAAGGTGGGCGGGTTGCCTGAGGCCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCC
ATCTCTACTAAAAATACAAAAAAATTAGCTGGGCGTAATGGCGTGTGCCTGTAGTCCCAGCTACTTGGGA
GGCTGAGGCAGAAGAATCGCTTGAACCTGGGAGGCGGAGATTGCAGTGAGCCGAGATCGCACCACTATAC
CCCAGCCTAGGTGACAGAGCAGCCCTCTGTCTCAAAATAAGATAGCCACAAGTGGTTGGTGGCTGCCACA
TTAGATGGTGCAGGGTTTTTGTTTTGTTTTGTTTTTTGTTTTTTCGAGGTGGAGTTTTGCTCTCATTGCC
TAGGCTGGAGTGCAATGGCATGATCTTGGCTCACCACAACCTCCTCTTCCTGGGTTCAAGCGATTCTCCT
GCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCACCACCATGCCTGGCTAATTTTGTATTTTTAGT
AGAGACAGGGTTTCTCCATGTTGATCAGGCTGGTCTTGAACTCCCGACCTCAGGCGACCCACCCACCTCG
GCCTCCCAGAGTGCTGGGATTACAGGTGTGAGCCACCATGCCTGGCTAAGATGATGCAGTTTTATCCAGG
TGGAGATGAAGAAATAGTCAGGGAAAGGTTGAGAGGCTGCATATTGTAGACAGATTGGATTACTGGGTTC
ACTGGGGAAGATTGGATTAGAAGGAGGAGTGGGGCTGCTTTACTTGTCCCCGACTTCTTGCCCCTGCTCT
GCCCACTCCTTCATTGAATACCCTGCTGCCCTGCAGATGTGTTTGCCTGATTTTCACCTCCCTTCCTGTC
TCTGTTATCATAGCAGACGTTTTAGTGCTCAGGGAAAGGAGGAGGAAGGAAACTCAGATTGTCAAGGAAT
TGTGAGTCAGTTTCTCAGCCACTACCCCAGGCCTGGGCTTGGCTAAGTCATTTCACCTCTCTGACCCTAG
CTAGTGGGGTGAATAGAGTCCCTACCCTGTTGGAAGTCAGGAAGCCCCTGGAGCTCGCTCTGTGCTAATT
ATCGGTGACTATTTCTGTGACCCAGCTCTTCAGGGGCAGCCTGTCTGGGGGTTCCAGTGCCCTGTGCAGT
ATCTCCAAGTGGTAGGTACTCAGTGTATGTATTAGTCCGTTTTCAGGCTGCTAATAAAAACACACCCAAG
ACTGGGTAATTTATAAAGAAAAAGAGGCTTAATGGACTCACAGTTCCACATGGCTGGGGAAGCCTCACAA
ACATGGCGGAAGGCAAATGAGGAGCAAAGTCACATCTTACATGGTGGCAGGCAGACAGAGTGCTTGCAGG
GGAACTCCCCTTTATAAAACCATCAGATCTCGTGAGACTTATTCAGTATAATGAGAACAGCACGTGAAAG
ATCCACCCCCATAATTCAATTACCTCCCACTGGCTCCTTCCCACAACATGTGGGAATTATGGGAGCTATA
ATTCAAGATGAGATTTGAGTGGGGACAGAGACAAACCATGTCAGTGTGTATTGTGGGATGAATGGAGATG
GTCATTACAGTCTTCTAAGTGTATGGGTCCATCTCTTACTTGCTAGTGTGTTAAGAAACCTGGCCTCCTT
TTTCACATCTTGAGGCACTGAATGGTTTACAAATCCAACTCTAATTTTTTATTATATTTAATACACCAAT
AGTGCAAGGCCTTTATTGGTAGTACTCTCCTATTACAGATGGAGAGACTGAGGCTCAGAGAGGTGAGGTC
TGAACTTGGAGCTCTGGACTCCAAAACCCAGTTTCTTTTCATGGTACCAGGCTGCCAGGAGGAGAAAGGA
TGGAGCTTAGATTCAAGTCTTGCAACATTCTTCTTAGTCCAGTATTGGCTTATTTTGTTTTATATTTTTT
TGAGACGGAGTCTTGCTCTGTTACCCAGGCTGGAGTGCAGTGGCGTGATTTCTGCTCACTGCAACCTCTG
CCTCCTGGGTTAAGCGATTGTCCTGCCTCCCCCTCTCGAGTAGCTGGGACTACAGGTGTGTGCCACCGTG
TCCGGCTAATTTTTATATTTTTAATAGAGACAGGGTTTTGCCATGTTGGCCAGGCTGGTCTCGAACTCCT
GGCCTCAACTGATCCACCCACCTCAGCCTCCCAAAGTGCTGGGGTTACAGGCGTGAGCCACCATGTGTGG
CCGGGCAGGTGATTTTTCTTGTCTTTCGCCTCTTTCCACTTCAGTCCCCCTGGCCATCTTTCCCTTCCTT
GAATGTGCCTCCTCCCACCTGGTGGCCTTTGTAGGTATGAGGTCCTTCAGATGATGGCTGACATGACACT
TCCTCAGAGAAGCGTTCCAGACCCCAAACACCAGGTCTCTGTCTGTGAAACAGGGACAGTGATACCTGCC
TAATCTGCACGCCTTTCCCTTCTCACGAGATATTCTGGGAAGGGGGACTCAACTCTTTGCTCTGTTTGAG
GGCAGAGTGCCTTTGAGCTCAGGAGAGAGTGGGTTTGGGTGGCCTTAGCTACCCTAGATACGGAGGACTT
GGAGTTAACTGGCACCATCCACAAAGCCTTGCATGCACTGGACCTGAACAAGTTGCTTAGCCCTTGAGGC
GGCGCCTCAGCTACCACCTGTCTTTGATGGAGTTATGATAACAGTGCCTGCCCCGTCTGGATGCCTTATG
GGTTCAGTGAGCACATAGGTGTAAATAAAGTGCTTTGAGTGGCACCTCAGCATGTAGTTATTCTCTGATT
GACCTATAGCAGCCATGCCAGTCTTCAGGGTCCCAGGGCGCCAGGCTTTCTTCTCTTGCCTTGGAGCCTT
GGTTTTTCCCTAGGACTGAAGTGTTCTGACCCCTTGGCTGAACTCCCCCTTCTTTAGGAAGCTTTCCCTG
GTGTGCCATCTGATGCTGGTGCTTCCTGGAGGGGTACTATCTGATCCTAGCTGGTGAGGCATTCCTGCCA
GAGCTGCGGCAGGTGCTTTCCCCGCCTCTCCACCAGCCTGTGAGGTGGATGCCATCCCCACGAGGGCACG
CAGGTTCACAGAGACTGGCTCCTGTCCAAGGCCGCGTGGCTGGAAAGAGACAGAACCAGATTCAAACACC
AGCAGTTTAACCCTAGAGACCCTCATCCTACCTCCTATGTGGGTAACCACTATACATCCTGCCTCCTCTG
AAATCTCCCGTTTTTTTTTATTTTAAAATTTTATGTGAGTATTTACTTATTTATTTGCTGCTGTTGTTAT
TGTTTTGAGACAGAGTCTTGCTCTGTTTTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGC
AACCTCTGCCTCCTCGGCTCAAGCGATTCTCGTGCCTCAGTCTCCCGAGTAGCTGGGACTACAGACATGC
ACCACCATGCCTGGCTAATTTTTGTGTCTTTAGTAGAGATGGGGTTTCGCCATATTGGCCAGGCTGGTCT
TGAACTCCTGGCTTCGAGTGAACTGCCTGCCCTGGCCTCCCAAAGTATTGAGATTATAAGCATAAGCCAC
TGTGCCCGGCCAAAATTTTATTTATTTATTTATTTTTTTGAGATGTAGTCTTGCTCTGTCACCCAGGCTG
GAGTGCAGTGGCACAATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGTTGACGCCATTCTCCTGCCTCAG
CCTCCCGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGA
TAGGGTTTCACCTTATTAGCCAGGATGGTCTCGATCTCCCGACCTCATGATCCGCCCGCCTCGGTCTCCC
AAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCCAGCCTAATTTTATTTATTAATTTATTTGAGATGG
AGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGTGCGATCTTGGCTCACTGCAACCTCCACCTCCCAG
GTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTATAGGTGCGTGCCACCACGCCTGGCT
AATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATCGTGATCAGCCTGACAGGCTGGTCATGAACTCC
TGACCTTAAGTGATTCACCTGCCTCAGCCTCCCAAATCTGCTGGGATTACAGGCATGAGCCACCGCACCC
AGCCATATTATTTCTGTATTTTCATTTGGCTTACCTGTCAACTTGACACTTTTTTGTTTTTTTAGGCTAG
TCAGGTGAAGCAATGTGAGTAAGATCACCTGTCTTGATTATAACTTGTGTGTTCAGTGTTTGTCATTCAT
CCCTCCCTGTCTCTAGCCTTCAGTTCAGGGCATGATTTGGAGCAAATGTTCAGTATCTGTTTAACAAGAA
CAATCATGAGAAATACTTACTCACTACTTGTGTTTATGAAACATTATGTGCCAGGCACTGGGCTGAGCAA
TCCACACAGGTCAACTCGGTTAATCCTCATGACAACCCCTGAGATAAGTACTTATACCACCCTCGGTGTA
CACAGGAGGAAACTGAGGCTCAGGGAGTTTTTCTTTAAAAATTTTTTCTTTTTTTTTTCCTTGAGACAGG
TCTTACTTTGTCACCCAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCACTGCAGCCTCTTGGGCTCCAGC
GATCCTCCCACCTCAGCCCCTCAAGTAGTTGGGGAGTACAGGTGTGCACCACCATGCCCAGCTAATTTTT
TGTAGGGATGGGGTCTTGCCATGTTATCCAGGCTGGTCTTGAACTCCTGGGCTCAAGCGATCCTCCCTCC
TTGGCCTCCCAAAGTGCTGGTATTACAAGTGTGAACCACCACACCTGGCCTTAAATTTTATTTATATATT
TATTTTTGTAGAGACGAGGTCTCACTATGTTTCCCAGGCTGGCCTCGAACTCCTGGACTCAAGTGATCCT
CTTGCCTCAGCCTCCAGAGTAGCTAGAATTACAGGCGTCTGCCACTGCACTTGGCTCAGGGAGTTTAAGT
CACATAATCAGGCAGCGGTCTGCTCAGTTAGAGTCCATGCGCTTAGCCATGGTGCCTTCAAGTGACTCCA
CCAGGGTCACCCCAGTACTTCCTGGGCAGACCAGACCATCCAGAGCGGGCAGGTTGTGCTTTCCTGCATG
GTGGGAGAGGACGTGGCATGAAGCTTTGTGTAAATCTGTGCAGCCTCTGCCATTGTAAATCACTGGCCAC
CGGGACAGACTCCCCCACCCCCTTCCAAAAACTAGAGGAACAGATGGGTGGAGTCGCATCCTTCCTCTGC
AGGCATTTGGCCACCCACTCCCATTATGGAGCATTTCCTCAGGACCAGGCACAGCGTGGAGCCCTTTGCA
TAGCTCACTGAATTGGGGCAGCAACCCTGGGGCTGGGGACTACCATTATCTCCACCAGCCCAGCAAAGAG
GGGAGGAGGGTACAGCCGAGACCCAGCTGCAGTATGTGGAGCACACTGGGGTGCCGTGAAGATAGCTGTC
CAGCCTCTAGAGGCTGACAGTCCTGCCCTGACACAGTGGTTTGTTCTCAGACTCTGGCCTCCCCTCTTAG
TGCCCGTCTGAAGACTTCAGCCTTCGGTCCTCTTCCATCAGCATGCCGTCTCCCCTGTGCCTCAGTCTCC
CTCTCTGGAGTATCTGCTGGGGCCACAGTTTGGTTTTATTGTTCTTTGAGACAGGGTATCACTTTGTTGC
CCAGGCTGGAGTGCAATGGTGCAGTCATGGCTCACTGCAGCCTTGACCTCCTGGGCTCAAGCCATCCTCT
CACCTCAGCCTCCCGCAACTCCAGTATCTGGACTACATGTAAATGCCACCATTCCCAGCTAATTTTTTTT
TTTTTTTTTAGATAGAGTCTCACTCTTGTCACCCAGGCTGGAGTGCAATGGTGCCATCTTGGCTCACTGC
AACATCCGCCTCCCAGGTTCAAGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACCT
ACCATCATGCACGGCTGTTTTTTGTATTTTTAGTAGAGACAGGGTTTCACTATGTTGGCCAGGCTGGTCT
TGAACTCCTGACCTCAGGTGATCTGCCTGCCTCGGACTCCCAAAGTGCTGGGATTACAGGCGTGAGCCAC
CACGCCCAGCCTTCCTGGCTAATTTAAAAATTTTTCAGAGACGAGGGTTTTGCCTAGGCTGGACTCACTA
TGTTGCCTAGGCTGATCTCGAACCACTGGCCTTAAGTGATCCTCCCACCTCAGCCTCCCAAAGTACTGGG
ATTACAGGCATGAGCCGCTGCACATCGCCACAGTTTGATTTTAGTATCCACTTTAAGGTGCAGTTAGTTC
TTCCCAGCTCCTCTTTTCCCTATCTCATACAGTGTAGTCACTTCCCTTTCTCCCACTTCTCACCTTCCTA
GATTTTGTTTTTCTCTCTCTTACCAAGTTTTGAGTATGTAATACGTCCCAGATCGGTGCTGAACCCCTGG
TGTGAGTTAACTTAGCAACTTTTCACGTGGTGTTCTGCAGCCTTCTTACAGATAAGGAAATTCAGAGAGG
TGAAGTGTTTTTGCAGAGAGCCACACAATGAGCTAAGAAATTAGAACCCAGGGCAGTTGAACTCCAGAGC
CAGGGAACTTAACAGCCCATTCATGCATTCACTCAGGAATTCCCTCTTGAGTGCCTGCTGTGAAGGCAAC
CTGGACGCTGTCTCTGCCTTCCTGGGATTGTGGTGTGTGTGGGAGACAGACACGAAACAAAGCAATCTTC
TAGGTCAGTGTCTGGCTATGCTGGGTACTCAGAAGGGGGAAGCACTGTGCTGCAAGAGGCCGGCTGTTTC
CTCCTCACAAGGGGAGGCCTGCAGTGACATTGCCAGGCCTGAATCTGCCCTGCCATTGGGAGTGTAATTT
CCTCACGACTACAAATGCAAATAAATACACACACTGAACATGCTACCCCGCTAGAACTGTGGACAGCTTG
TTCACTGCAGTTAAAGGAAGACGGAGGGACACATTTGAGCACGCATGTGTGAGATCCAGCTTGTGGCAAA
CCCTGCCATTCTTTAACATCTGTGGGAAGTGAAGCCACCATTACTGACCTCGGAGGAATCCCGGGAGTAG
TGGGAAGAAAAGTTGCAGATGTTTGAAAATCAGGCTGCAGGGGCAGTTGGGGTGAGGAGCATGGATGTCT
AGGAGCCTGAATCTCAGCGTTGGAAGCCCAGTTCTGATGTAATTTTTTTTTTTTTTTTTTTTTTTGAGAC
AGGGTCTCACTTTGTCCCCCAGGCTGGAGTGCAGTGGCACAGTCTCAGCTCACTACAGCCTCATCCTTCC
AGGTTCAAGCAGTCCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACCACAGGCATGTGTCACCATGCCTTT
CTAATGTTTGTATTTTTTTGTGGAGACAGGGTTTCTCACTGTTGCCCAGGCTGGTCTCAAACTCCTGAGC
TCATGTGATCCTCCTGCCTTGGCCTTCCAAAGTGCTAGGATTACAGGCGTAAGCCACTGCCCAGCATCCC
CACCTTTTTTTTTTTTTTTTTAAATTTGTGGTTTGGTGCAGGTTATTTTACCTCCCCAGGCCTCGGTTTC
CTCATCTACTCAAGGGCAATGGCAGATAATCAACCTCACAAATTCCCCGAGGAGTTAGTAGGCTTGTGCC
TGCCAGGCTCAGCTGGCTTACAGCAGGTGCTTCATAAGGGGGAGCTATTGCAACGCCTCGGCTGGTTCTA
GGCTGCCTAGCTCCAGAGCTAAGAGTCCCAGTGGGTTCATATCCCTTTGAGCATTTATAGGCTTCTCACA
GGTCTCCTGGGCTTTCTCAGCCATTCCTCATCTGACCTGAGGAGGTGTGATGTCCTTGCAGGACTCCTCC
AGCCCCTCTGAGCTTTTTTTTTTGTTTGAGACAGCATGTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGT
GTGATCATGGCTTACTGCAGCCTCCACCTCCTGGGGTCAAGCGATTCTCCCACCTCAGCCTTCCAAGTAG
CTGGGACTATAGGTGTATGTTACCATGCTTGAGTAATTTTTAAAATTTTTTGTAGAGATGTGATCTCAGG
CTGGTCTTGAACTCCTGGGCTCACCTGGGCTCAAGTAATCCTTGAGCCCAAAGTGTTGGGATTACAGATA
TGAGCCACCTTGCCAGGCCCTGAGCTCCTTCTGTCTTCCATGAAGGACACACTATTCCTTGCCTTTTGTC
TTTTTTTGTGTGTGTGGCAGGGTCTCTGTTGCCCAGGCTGGGATGTGTTGGTGCAATCTGGGCTTATTGC
AACCTCCGCTTCCCGGATTCAAGTGATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACAGGTGTGC
GCAACCACGCCCAGCTAATTTTTGTATTTTTGGTAGACATGGGGTTTCACCATGTTGGCCAGGCTGGTCT
CAGCCTCCCGAAGTGTTGGGATTACAGGCATAAGCCACTGTGCCTGGCCACTTTTTGTCTTTTGCATGTT
CTATACACCTTGCCGAGAATGTGCTTTCCCTGCCTCCTGGTTATCCCTCAGGTTTCAGCTCTAACATCAC
TTCCCCAGGGAAGCCTTACCCTCGCTTTCTTCTGCTATAGGATAATATGAACCACCATCATTGTTACTTG
TTTAGTGTCTGCCCTCCCTGCTAGGCAGCAAGGACAGATATCTTGCCTGTCTTAGGTCCCCAGAACCCAG
CACAGGGCTTGGAACATGTCAGGTGCTCAAATATTTGTTGAATGAGTCAGTCTGGGCTCGCAGAGGGAGC
TGGGACCAGAGTAGAGTGGACACTGAAGCGTATGTAGCAGTTTGCCAGGCGATAGGGAAAGAGACCTTCT
TGTAAAGCAAGCAGAGATGAGAGGGAGAAATGAGAATTGCTAAGATTGTTTTGAGCATTTGTTAGGTGGC
TTACAGATGCTTTGCTGAACATTTTATTCATGTGATCTTGTTTAAGCCTCTCATTTCCATGAGGGGTTCT
CCATTTTTATAGACAAGGAAGGGGACACGTGGAGAGGGGAAGTTACTTTGCCATATCACCCTCTAAGGGA
TTTTTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTCACTGTCACCAGGCTAGAGTGCTATGGCGCAATCT
CGGCTCACTGCAACCCCCACCTCCCGGGTTCAAGTGATTCTCTTGCCTCAGCCTCCTGAGTAGCTGGGAC
TACAGGCATGTGCCACCACATCCGGCTAATTTTTCTATTTTTAGTGGGTACAGGGTTTCGCCATGTTGGC
CACATTGATCTCGAACTCCTGACCTCAAGTGAGATTCGCCTGCCTCTGCCTCCTAAAGGCAGTAAGGGAA
TTGAACCTAGGCAGTCTGACTCTTAAGAGCTCAAAGACTTAAGCTCTGTCCTGAGTAGCTTCGCTGGTGT
ATGGTATAGGGATCAGAAGAGAGCTGGTCTGTATGCCTAGAGGGCATCCTGCAGTGGGCATGTGTGTCTG
TGGTCACAGTGGGAGAGAGAGAGGAGGGGGCATCTGTGGCCAGAATGGGGAGGCCTCGAATGCCAGGATG
AAGAGTTTGGGCCTATGGTAATGAGAATAGTAATAGCTACCAGTTATTGACTTGCCAGCCACTTGATTGA
GTCATTTAACCTCACAACAGTCCTATGGGATAGGTGTTAACTGTTTTCATCCCAGTTTTTCAGATGGGGA
AACTGAGACACAGAAGTTGTAGTCAGTTATCCAAAGTGTCGCAGTGAGAAAGTGCTGGAGTTGGGGCTCA
GACCCTGTGAGTCAGGCCCCAGAGTTCACTGGGGCCACATGCTCAACCACCATGCCGCCTGCCTGCCGCC
GGGGCTGAGCCAGCGGAAACCTGGGTGCTGCTTCTGATGCAGGTGGTCTCCTCTGCTCTCTTCCCCAGCA
CCCCCCCAGGCAGCAGCAGCAGTTTTCATCTCTGGATGACAAGCCCCAGTTCCCAGGGGCCTCGGCGGAG
TTTATAGATAAGTTGGAATTCATCCAGCCCAACGTCATCTCTGGAATCCCCATCTACCGCGTCATGGACC
GGCAAGGCCAGATCATCAACCCCAGCGAGGACCCCCACGTGAGAGGCGGCCTCCCCCACTTCCCGTGCCC
CCCACGCCCAGGCCCCTTGCCTGTCTCCTCTCTGGTCCCAACTGCCCCACGTCTATCTGTGCCTCCACCC
GCAGCTGCCGAAGGAGAAGGTGCTGAAGCTCTACAAGAGCATGACACTGCTTAACACCATGGACCGCATC
CTCTATGAGTCTCAGCGGCAGGTGCGTGGGGACAGGACTAGGGGCGGGGGGCTGGAATTACCTGAGGTCC
CCTACCTGTGTTTGGGCCAAAGGAATGGCTCCCAAGGAGGACAGATTCTTTTTTGGGGGGGTTCCATAGA
GTTCTGAGGGTTCTCTTGGGACTCTGGAGGAGAAGTGTGAGAGCAGTCCTGCCTTAGGGCAGAAGGATGG
ACCTGATCACTAAAAAACACCTACTGTGTGCCTGGCTGGGGGCTCGCCTGTGTGGGGAGAGGGCAGGGAG
AAGGCCTGACAGCCCTTCCTCTTGGAAATGCACAGTCCTGAGAAACAGGGTGGGTCCCCAGTGCTTCCTG
GAGTGCTCCAGGGGACCGCCCTAGGCCCATGTGAAATGGGCTGTGATCCCCTGATAAATCCTAGTATCAG
GGACAAGGATCCAGTCAAGCCAGTGTCAACAAAGAGGGAAGTTATTGACTCCAATGACCATACATCCAGA
GGGTCCTGTTTTGGGGCACAGCTAGATCCAGGGGGTCTTCCAGGCAGGAAGTAGAGGGAAGGCGGAAGGC
CAGACATGGCACATCCCAGCTATCCCACACCCCCACCTTTATAGAGCTTTCTGGGTAGTCCCACCTAGTG
ACATCTGCATACATTTCCTGGGCCACTCCTAGCTGCAGGGGAGACTGAGAAATGTATTAATAATACTTTA
GTCCCGGCAGTTGCTGCCTGCGGGAGATTGAGACCTCTGTTAACAGAGGAGGGGAATAGTATACTGGGAA
GGCAACCAGGAGCCTCTGCCAGTAGCCCCGAGTCCTGATCCTCAGGGGGCTGGTTGCAGGCTCTTACCCA
CGCTAACCTGGACAGTCCTGCCAGGCCCTCAGTACTCAGCACCGCCCACTCACTTGGCACTGGAATGTGC
ATTAACTTTTAATTGGCTCCCCACTTAGAAGAAAGCACACATTCATCAGTTTCCCCCACAGCAACGGGAA
AGCCAGGCCTGGGAACACATGGAGGAGGGCACCGCCGAGCCGCGAGGAGCTCTGGCCCCGGGGTGTCCCT
CAGGGTGCTGGCCAGGCTGCCTTTGTTCCGCTTTACACATGGCCCTGTGTGATCTCATGTCCATCCGTAT
GCCAGGGCTGCCCAGAGCCCCAGCCAGACTGCTTGTCAGAGCCCTGCTTGGCTTCTCGCCCTGGATGTCC
CACAGCAAACTAATCCTGTCTGAACTTGACCTCGACATCTTCCTCCCAGGCGTGCTCCTTTGCCTCACCT
AGTCCGTTTCTCAGTGAAGAGCACCACCATCCACCTGCTTGCCCACACCAGAAACCTCATCTCATCCCTG
TCTCCTCCCTCCCCCTTGCTATCCCTTTCTCCCCTCTCATTCCCTCTGTCTGTAGATATTTCTTTTTTTT
TTTTTTTTTTACTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCACGATCTTGG
CTCACCGCCTCCCTGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCG
CCACCACACCTGGCTAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCATGTTGGTCAGTCTGGTCTCG
AACTCCCTACCTCAGGTGATCTGCCTGCCTTGGCCTCCCAAAATGGTAGGATTATAGGTGTGAGCCACTG
CACCCAGCTGTAGCTACTTCTTTTCTTTTATGTTATTTTTATTTATTTATTTAGAGACGGAGTCCACCTC
TGTCGCCCAGGCTGGAGTGCAGTGGCGCAATCTCGGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGTGA
TTCTCCTGCCTTAGCCCCCCAAGTAGCTGGGATTACAGGCGCCCACCACCACACTCGGCTAATTTTTGTA
TTTTTAGTAGAGATGGGGTTTCACCACATTGGCCAGGCTAGTCTGGAACTCCTGACCTCAAGTGATCCGC
CTGCCTTGGCCTCCCAAAGTACCGGGATTACAGGTGTGAGCCACCGTGCCTAGCCTGTCTGTAGATACAT
CTTTAGGAAGTGCTCAGCTGCTCAGCAGCACACTCTGAGACCCAGCCCCATCCTTTCTCTGCTTCACTGT
TGTTGGTGAGTTGACCTTCGTGCTCTGCTTGTGGCCTCGTGTTCACAGCATGGCTGCTGCATGTCCAAGC
TTCATGGCTGTCTGCAAGGCAGGAAGAAGTAGGGAGTGGGTAGGGCTGGCACCAGCAGCAGTGGTCTCTT
CTATCCAGAAAGCAAAAGCAGACTTCCACCAAAATATCTGGCTAAAACTGTGTCCCATGGCCACCTCTGG
CTGCAAGGGAGGCCAGGGACCTGAGTGCGTGTCTTTTTGAGGCTCAGGTTAGGGGATTTGGGAGAAGGGA
ACTGGGCACTGGTGTGGCCAGCTGACAGGGTCTGCTGCCCCCTAGCACAGCCGACTCAGCTTCCTCAGAC
ACATCCACCTGCTCCTCTCCCGCCTCCCTCTGCTACCAAGCTGGGCCTCTGCCCCCTCTCTCCTGGCTTG
CTGCAATCAGCCTTGATGACTCCATCCATTTACCTCACTGCAGCCTGAGAGCAATTCTAAAACACAGGTC
TGACCACATTGCTCCCTTGAGAAGACTGTCGGCAGGTTACCACTATTCTCAGAGTAAAATCAATGTATCG
TTCCCTTTGTTATCTGGGTGCCATCTCCTCTCCCTCCTCATCTTTCCCCACCCCTACCACTGTATGTCCC
AGCCCTTCTGGCCTCATGGTCTATCATTCCCTCTGCCTGGAACCCTCCACCCACCCCATCTTTGCGCACT
GGTCCTCACCGATGCCCTTCCGTGGTGGAACAGGTGCTTCCTTTGGGCTTCTATCACAGCAACTCGATCC
CTCTGGCAGTTCTAAGCAGTCTGGCAGCGTCTTCTTAAAGGCAGGAACCCCAGCATGGCCTGGCCCAGGA
CTGGCCTTCAGGAGGACTGTGAATTCATGGAGGTGTTGGAAGCTGGGCAGGATTTGGATGGCTCTCTGTC
ATTGCCCAGCATAACCAATTGTGGGACCCCGGTCCCCTCTACACCCCCAGGGCCGGATCTCCTTCTACAT
GACCAACTATGGTGAGGAGGGCACGCACGTGGGGAGTGCCGCCGCCCTGGACAACACGGACCTGGTGTTT
GGCCAGTACCGGGAGGCAGGTACGTCTGTCCGTGGTTTGGCCCTGTGGTCCCCATTGAAGTGTACACTTT
AGTTTTTCTGAGTGTTCTTCCAGGAGCAGCATAGTGCCAGGAAGGTCTAAGGAGCTGGAAGAAGAGCTTT
CCAAGCAAGAAAGAGACAAGCCAGGGTTAGGACTCTGGAGGGGGCAGTCTTCCCACTCTGAAGGCCAGAA
AGTGTAGTGGCTCAGGGTCTGTGCTCTTAACCCTTTGTTACCAGTTGTCAGGTTTTGGGCAAGTCACTTC
CCTTCTCTGAGCCTCAGTTTTCTAATCTCCAGAAGAGGGATTATGACGGTACCTGCATCCAAGGGTCGCT
GGGAGGATGCAGTGAAGTCAACGTTCGCTGTGGGTGTGGCCTGTGCAGGAGACCCTGTCTGCCTGGTGCA
GTGATCCCCTGGCCCCTCCTCAGGTGCTGTATCCTTCGGGGAGCAGGAGCAGGTGGAAGAACCTTGACAG
ACCTGAGTGCGGTGCAAGATTAGAGGGGCCCGGCAAGCCCAGATGAGTGAAAGGAAGGAAGGGTCAAAGA
CCAGGAATGAAGAAAATCATTGAGCAGGAATGAGTGCCTGGGAGCGAAGGAGTGAGCCCAGGGAGCAGTG
AGTGGGGTCTTCCAGGGAGCTTGTTGGTGTGGGGCCCTGCATGGGGACTACCCTCAGAGTGTCAGCGAGC
ACTGATAGGGCCAGGAAGGCATATCTTAACCCTTTGTCTTAGATGCTTGGGGCAAGTTGCTTAACTGCCC
TGTGCCTCTGTTTCCTCATTCGTAAGGGGGATGGTAGCCCTTCCCAAGACAGCTGTTGAGGGCAGTCTTG
GGGCAGTTGGCAAAGTGCCCTCAGCACAGAGTAAGCGCTCAGGGCCAGGGGTTGGTGTCATGATCGGGGA
CTCGCCCTGGGACAGCCCTTCAGCAGGGCCAGACCACGTGGCTGTCCAGAGCCCCCATGGACCAGCATGG
CTTGCAGCCTGCTCCTGGACTGTCTACTGCTCACAGCTGGACCAGGCCCTTGGCCCCACATGCTCTAGGA
GGGCAAACGCCCTGTGGTGTTTGTCTAAGGTCTAGTGTCATCCGTGCGGAAAAAGATTTCCAGACACCAG
GCAGCAAGCTGGGCCCCCGCCAGCTGTGCTGAGCACTGCACCTGTTTTTGCTGAAGGGTTCTCTTGTGAG
GGCACCAGGCAGGACCGAGCAAGCCTCATGGGAATCATGGGAGCCGCTGAGCTCTCCACATGGGAGAGCT
GGCCGCCTCTGCAAGAATGACTGTCCAGCACTGAGGGTCAGCAGAAGTGTCTGGGCAGCTGCAAATGGGA
CTCATGCAGAGGCAGCATGGTGCAGGGTAAGGAGTTGAGTCACATCCACTCTAGACTTCACTTGGCAGGC
CACTCACCTTGGTGACGCTGTCTGTCGTCTGTGAAGTGAGAAGAGTGGTGTTCATCATGATAGGTAGTGC
CTCCCTGGGCTCTTGACACCAGAGTGCTGTTCTTTTTTTCTTCTCCTTTTTTTTTTTTGAGATGGAATCT
CGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCCACCTCCTGGGTTCA
CGCCATTCTCCTGCTTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCACCACCATGCCTGGCTAATTT
TTTGTATTTTTAGTAGAGGCGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTTGCGATC
CGCCCTCCTCCACCTCCTAAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCTGGCCTTTTTTTTTTTT
TTTGAGGTGGAGTCTCACTCTGTCGCCCAGGCTGGAGTACGGTGGCGCAGTCTTGGCTCATTGCAACTTC
TGCCTTCCAGGTTCAGGTGATTCTCCTGCCTCAGACTCCGGAGTAGCTGGAATTAAAGGCATGCACCACC
ATGCCTGGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCACGTTGGTCAGGCTGGTTTCAAACT
CCTGACCTCAAGTGATTCGTTTGCCTTGGCCTCCCAAAGTGCTGGGGATTACAGGCATGAGCCACCGCGC
CGGGCCAGCCAAGGTGCTGTTATATGTGCTCTTTAGCCTCGGAGACTGTCATTTCCCCTCACTGCACTGC
AGAGCGAGGGCACACCGGGTGAGGTTAGGGCAGGCCCCAGTGGTGTTGCCTCATTGAGGCACCTCCCCCA
TCCATGTGAGGCAGGAGAGGCAAGCATTACCATCAACTCTAAAAGCCAGGTAAGAACCTGAGTCAACAAA
GGTGAGGTCATGGGCCCAGGCCACATTCTCCTAAGGAGTGGAGCCGAGTCTTTTTATGGCAGTCTGACCC
AGAGACAGCTCTCGCTGTTTCCCACCCTTGGCTGTCTTCCCAGGGCTATATGTGGAGTATCAGTGGTGGC
ACTGGCAGGGAGTGGCGCCCGGTGAAAAGGAGTGTTAAGAGGAAGCACGTGCCAGGTGTGGTGGCTCACG
CCTGTAATCCCAGCACTTCGGGAGGCTGAGGCGGGAGGATTGCTTGAGCCCAGGAGTTTGATACCAGCCT
GGGCAACATGGCGAGACCCCCATCTCTAAAAAATTAAAAAATTAGCCAGGCGTGGTGGTGCACACCTGTA
GTCCCAGCTACTCAGGAAGCTGAGTGGGAGGATTGCTTGAGCCCAGAGGTTCAAGACTGCAGTGAGCTAT
GATTCCACCACCGAACTCCAGTTTGGGTGACAGAGTAAGATCCTGTCTCTGAGACCAACAAGAAAAGAGG
TAGCATGTGTCAAGCAAGAGCAGCAGTTCCCACTGGACCTGGAGTTTCCTGGTGCAAATCAGCCTGTGAG
TCTGTATTTTCTTTTTTCTTCAAGACGGGGTCTTACTCTGTCACCCAGGTTGAAGTGCAGTGGCATGATC
TCGGCTCACTGCAACCTCTGCCTCTTAGGATCAAGTGATCCTCCCACCTCAGCCCCCTGAATAGCTGGGA
CCACAGGTGCACGCCACCACACCTGGCTAATTTTTTTTTTGATTTTTGGTAGAGACGGGGTTTCGCTGTG
TTGCCCAGGCTGGTCTCAAACTCCTGAGCTCAAGCAGTCCACCTGCCTCACCCTTCCAAAGTGCTGGGAT
TACAGGCACGAGCCACTGAGCCTGACAAGTTTGTACTTTTAGCACGTTCCCCTGCCCCTGCCCAGTCCTT
CTCACATGGGTGGACCTAGTACCACATTCTGAGAAAGCCTGATTCCAAGGTCCTGTATCAGTCCCTTGGA
CCTTCTTTGACCCTCTACAAGACCTTTCTTTCCCTCCTTAACCAGAGCAACTCCTGTTTCTATCTGTTAC
ATATGCAGCACAGCCACATCTTGTGTGGATTTGTATTATGTCCATTCAAAAGCATGTGTCAGAAAATACC
AGTAAACCTGTATTAAAGTTGGATGTTGAAGTAGTGCTAACCACCCTCAACTCTAACTAAAATATCAACA
AGTGGGCTGGGCGTGGTGGCTCACACCTGTAATTGCAGCACTTTGGGAGGCTGAGGTGGGTGGATCACCT
GAGGTCGGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTCCTGAAAATGCAAAAATTA
GCTGGATGTGGTGGCACATGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCGCTTGAACC
CAGGAGGCAGAGGTTGCAGTGAGCCAAGATTGCGCCATTGCATTCTGGGCAACAAGAGCAAAACTCCGTC
TCAAAAAAAAAAAAAGAAAGAAAATTAGGTAGATCTAGTGGTGCACACCTGGAATCCCAGCTATCTGGGA
GGCTGAGGCATAAGAATTGCTTGAACCTGGAAGGTGGAGGTTGCAGTGAGCTCAGATTGCGCCACTGCAC
TCCAGCCTGGGTGGCCAAGCCAGACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAGGTAACTGCTGT
GTGTAATGTGATCTCCATTAAATTTGTATCTAAAGAGCGGGTTCTGCGGTAAAGAAAGGATTTGGAAAGC
ACTGTACTAGAGAGCTCCCTCTGAATAATGTTCCCGGTCTGAGTGCTTGCAGCATTGATCCACAGCGCTG
CCCCTAAAGTGTTGGTGTGAAGGTTGTCCCACTCTTCAGGCGGGGCAAAGAGCTTGTCTGAGGGCAATCA
CAAACCCCCTTCTGTCTTCTAACTTCGGAGGAAGGGCCCCCTTAACCTGGAGCGGTTCCCTTTGATGGGG
AGGTGGGTGTTCACCAGAGGTTTTGTTTGAAGAAAAGGTTTTACTGCCATACATGGGTTAGAGTACTTCT
GTAGTCCAGAAGTTGTAAATTAGTGGTCTATGCTGTTATTTGGTTCATAATGATGATTTTTTTTTTTTTT
CGAGACAGGGTCTTGCTTTGTAGCCCAGATTGGAGTGCAGTGGCACGATCTCAGCTGCCTGCAGCCTTGA
ACTTCTGGGCTGAAGCAATCCTCTGCCTCAGCCTCCCAAGTAGCTGGAACCACAGGCACACATCACCATG
CCTGGCTAATGTTTTGATTTTTTTTTTTTTTTGTAGAAGCGAGGCCTCACCATGTTGCTCAGGCTGGTCT
CCAACTCCTGGGCTCAAGCGATCCTCCAACGTCAGCCTCTCAAAGTGCTGGGATTACAGGCATGAACCAC
CATGCCTGGCCATAGTAATGATTTATGGTTAGAATATTTCTTAAAGGTAATTGAATTAGTTGAAAGCACT
TAAATACCAGACAATATCTCACTAAGAGAAAGAAAGAAAAAGGCTAGATTTCTGCTTTCTTTTGAAAAAT
CAGATTGGCCTTGCTGGGCAGAGTCAGTCAGTCTGAACATCAGTCTTCCTCTTAAAACAAGCCTGAGCTT
TCCTGTCTGCCTGCCAGCATGCTGCAGGTCACCCACAGGGCTGAACTGTCCCCCTGTACTGCCCACTCGG
CTAACCATTGCCTCCTCCCCTCCTAGGTGTGCTGATGTATCGGGACTACCCCCTGGAACTATTCATGGCC
CAGTGCTATGGCAACATCAGTGACTTGGGCAAGGGGCGCCAGATGCCTGTCCACTACGGCTGCAAGGAAC
GCCACTTCGTCACTATCTCCTCTCCACTGGCCACGCAGATCCCTCAGGGTGAGGATGCATGCCCTGTACC
TTGCACATGTGCAGACCAATGTCACACCCCTGTCCAGGCCTCAGCTCTTTTGCCTGCCTTCTGGGTGGAA
TTCTGGGTTGGTGACACCACTAGAGCCCTGGTCTGTGCTCCAGACTTCTTCTTCTTATTTTTTTCTTTTT
TGAGACGGAGTCTTGCACTGTTGCCCAGGCTAGAGTGCAGTGGTGCTATCGCAGCTCACTGCAGCCTCCT
TCTCCCAGCTTCAAGCAATTCTCCTGCCTCAGCCTCCCGATTAGCTGAGACTACAGGTGTGCACCACCAC
GCCCAGCTAATTTTTGTATTTTTAATAGAGACAGGGTTTCACCGTGTTGGCCAGGCTGGTCTCAAACTCC
CGGCCTCAGGTGATCCACCCGCCTCAGCCTCTCAAAGTGCTGGGATTACAGGCGTTTAAGCCATTGTGCC
CAGCCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTCTGAGACGGGGTTTCACTCTCGTCAG
CCAGGTTGGAGTGCAATGGTGCAATCTCGGCTCACTGTAACCTCCACCTCCCAGGCTCAAGTGATTCTCC
TGCCTTAGCCTCCTGAGTAGCTGGGATTATGGGCATATGCCATTGTGCTTGGCTAAATTTTTGTATTTTT
TGTAGAGATAAGGTTTCACCATTTTGGCCAGGCTGGTCTTGAACCTGAGTTCAAGTGATCTGCCCACCTT
GGCCTCTCAAAGTGCTGGGATTACAGATGTGAGCCACTGCACCTGGCCTGTGTTCCAGACTTTGAATGCA
CCTCTTGTCTGGGACCCTTCAGAAGAGGGCTCTGTTGCTGTCCCCACCCCATTCAGGGGCCTGAAGCCTG
TGTGACCACACTCTGGATTTCCCAACCACATGCCTACTGCATTTGTCTGCTCACAAACATAGCCAACGTG
TGCACCAGGTTCCACTGTGCCAATGCTTCACGTTCATCATCTAATAAGGACCCTCATTGTGGCCATGGGG
ACAGAGGTTATTATTTGCTCTTGTTCCTGTTTTCAAGAGCAGGATGCCCGTGTTTTGTGTGTGTGTGAGA
GACAGCAAGTGAATGGGAAAAAAAAAAGTGCAGGAGCCTGGCCAACATGGCAAAGCCCTGTCCCTACTAA
ACATACAAAAATTAGCTTGGCATGGTGGTGCACACCTGTAATCCTAGCTACTCAGGAGGCTGAAGCAGGA
GGATTGCTTGAACCTGGGAGGCAGAGTTTGCAGTGAGCTGAGATCGTGCCATTGCACTCTAGCCAAAGTG
AGATCCTGTCTCAAAAAAAAAAAAAAGGAAATTGAAGTCAAAGAGGGAAGTCACTCGCTAACGGTCATGT
GGCACAGAGGGAGAGGAGCAGGGACTCAAGTCATTGACTGTGTACCTCCAGAGCCTGGGTTCTTGGCCCT
GACCTTTGTCTGCCTCAGTCCTCAGAGTCAAGTAAGCTTCCCCAAAGCTTACCGTAGGTGCCCTGAAGTC
CCTTCCCTTATAAGAGCTTTTTTGGGAAGCAAACCCACTGGGTGCCAGGCCTGCACCCTCATAGTGTGGA
GAACAAGGAATCGGAGCCCCTTCCTGTCCTCCGGGGCCTGTAGCCTGATCTAGGGTTGGTCTGGGCCCTG
GCTTCTTTGCCCTAGGGCAGCTGAGATGCATGAGTTTGTGTCTCTCCAGGCCTCAGCACTGTGCCCAGCA
CAGCAGGAGGCTCCACACCCCTTAGCTCATAGTGTTCTTTTGTTGAGGAACAAGAAGCAGTTTGAGCCTC
TTAGGTGGGAGGTGGGCAGGGAAGCTTTCTCAGAGGAGGGGGCATTTAGGGGGCATTTGAGCTAAAGGTA
ATAGGGTTTCCACAAGGCTGGGGCGTGGCCCTAACCTTCCCTCTGGGTCCCTGCTAAGTCACTTCAAATA
CACGAAGTTCCTGCCATGTGCCGGTGCCACTCCAAGTGCAGGGAACAAAAGCAACGCAAACCTCAGCCCT
CCTGGTGCTGCCATTGTGGTGGGGGAGACGGACAGTAACAGAGATGGAGAGGGAACTCCTGGAGTATATG
AGGGTGGGGGTCCTGTAGGGAGCAGCCAAGCCAGCCGGGTGTGGTGCACTGTAGGGGTGGCTGGGGTCCG
TCACTGGAGTGACACTGAAGTATGGACCAGAGGATGTGAGGGGGGAGCTGTGTGGTGTCTTGAGGCAGGT
GTGAGCCTGGCGTGTTTGAGGAGCTGGGAGGAGCCCAGAGGGGCAGGTGTGGAGTGAGCAGGTGTGGAGT
GAGCGGGTGTAGGGTGAGCAGGTGTGGGGTGGTTGGAGGTAAAGTCCAGGAGGTGGTGGGTGGGGGTGCA
GATGGTGCAGGTTGGGTGGGGCCTCAAGGCCTTTGCCTGTTGCAGGGATGTCGACTTTTACTCAAAGTGG
GACAGGAGCCGTGGGTGGGGAGGAGTCTGCTCTTCCTTGGCCTGGCTCACCTCTTCATGGAATCCGCGGG
CTGCCCTGTGGATAAGGGATTGGGCAGTGTGTGAAGGGCTAGTAAGGAGGCTGCTCAGTAACTTGGGCTT
GACCAGGGTGGTGCAGAGGAGGAGGTAAGAGGGGTTAGGTTCTGAAGGTGGAGTAGATGTGGAGAATGGC
GGGAAGTTTGGCTTGGAGATGATACTGGACTCTTCAGCCTGAGCAGTTGGAAGGGTGGAGTTGCTTTCAG
CTGAGCAAGAGGAGACCTGGGTGGGCAGATGCTGGGGGGCATCAGGAGCTGAGGTGTTTCCTTTCCCCTT
GAAGCCTGGTGACTGCTGGCCTGAGCCACGCTTGAGCCGTGGGTCATGTGAGTGTGAATGAGTGTGAGTG
CATGTGAGTCTCCGCCCCTGCTCACCACCCTCTCATCCCCTGCAGCGGTGGGGGCGGCGTACGCAGCCAA
GCGGGCCAATGCCAACAGGGTCGTCATCTGTTACTTCGGCGAGGGGGCAGCCAGTGAGGGGGACGCCCAT
GCCGGCTTCAACTTCGCTGCCACACTTGAGTGCCCCATCATCTTCTTCTGCCGGAACAATGGCTACGCCA
TCTCCACGCCCACCTCTGAGCAGTATCGCGGCGATGGCATTGGTATGGGCTCTGCTGGCTGCTCCCCACC
CCGCTGGGATCATCTCCTTCCCTCCCCAATCCTGCCACCTTCCTGCCACCCCTACCCTCCTTCCTGGTTC
TCGTCCTGTGTCCTGTGGCGTCTGGCACTTGGTCAGCCACAGGAGTTGAGGTCCTGAGCACTCAGCCTTG
CTCTCTGTCCTCTCCCTGCTCGTCCCCTTGGCCTCGTGCATGTTCCTTATCTCAGCCCTGGCCTGACCTG
CCTTCTCTGTGTCCCCACAGCAGCACGAGGCCCCGGGTATGGCATCATGTCAATCCGCGTGGATGGTAAT
GATGTGTTTGCCGTATACAACGCCACAAAGGAGGCCCGACGGCGGGCTGTGGCAGAGAACCAGCCCTTCC
TCATCGAGGCCATGACCTACAGGTGCCTGCCGCTCCCCCCGTCAGCACCCCCACAGCACTGACAGCCACC
GTAGCATCTTCCTCATATCGATCACTGTCTCCAAAACATGGCCTTATCACCTGATGTTGCATCTCCCCCT
TGCCTTTATTCCGTTTCCACTCCTCCTTCCCTAGTTCATCCCCCATCCTCCCTCCTGACCCCCACTCCAG
GGAGCCCACACTGACCTGGGGCCCCTTGCCCCTGTGCAGGATCGGGCACCACAGCACCAGTGACGACAGT
TCAGCGTACCGCTCGGTGGATGAGGTCAATTACTGGGATAAACAGGACCACCCCATCTCCCGGCTGCGGC
ACTATCTGCTGAGCCAAGGCTGGTGGGATGAGGAGCAGGAGAAGGCCTGGAGGAAGCAGTCCCGCAGGAA
GGTGAGGGTGCCCCGCCCGGGAGGGTGTGCTGGGGGCTGCTGCGGCCTGCAGAGCTTGGGAAGGATTTGT
GGAACACCGAACTGGGAGGCTCAGGGATAACCCCAGTGATGTCTCAGATGTGGCCTGTGGAGCCAGGCTG
CTGGGGCCATGTGTGTCCTGGCTCTGTGTCCTTGGCATGTTGCCCTCCACTTCCTTGTCTTTGAAAGGGG
ATGCTGGTGGTGCCCATTTCAGAAACTGGTTTGAGACTGGGCATGGTGGCTCACACCTGTAATCCCAGCA
CTTTGGGAGGCCGAGGTGGCAGATCACTTGAGGTCAGGAGCTTGAGAACAGCCTGGCCAAACATGGTCAA
GCCCTGTCTCTACTAAAAATACAAAAATTAGGGCTGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTT
TGGGAGGCCAAGGCGGACAGATCACCTGAGGTCGGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACC
TCGTCTCTAATAAAAATACAAAAATTAGCCGGGCATGGTGCCGGGTGCCTGTAATCCCAGCTACTTGGGA
GGCCGAGGCAGGAGAATTGCTTGAACCTGGGAGGTAGAGGTTGCAGTGAGCTGAGATCGTACCATTGCAC
TCCAGCCTGGGCAACGAGAGCAAGACTCCATCTCAAAAAAAAAAATTAGCCAGGCGTGGTGGTGCGTGCC
TGTAGTCCCAGCTACTCAGGAAGGTGAGGCAGAAGAATCGCTTGAACCCGGGAGGTGGAACTTGCAGTGA
TTGGAGATGGCGCCACTGCACTCCAGCCTGGGCAACAGAGCAAGACTATCTCTTAAAAAAAAAAAAAAGC
TGGTTTGAGGATTAGGTGGCAAACCGTGGTGGAGTGTACATGCCCTGCCCAGAGTCAGTGTGCCACCCTC
CCTGGCCCGGTCATTAGGACCCAGGGCCCGTGCAGCCCAGTGGTGAGGGCAGCACCCGGCATAGGGCAGC
ACCGTGTGTCCTGTCCCTGCCCTTCTCTGTGCCTCAGTTTCCTCATTGCTTAGCTGTCTACCTCTTAGGG
CTGCTGAAAGCCTTAAATGATTCCACACACTTGGATAGCACCTGATCCCTGCTGGGATTTGAGGGTTTTC
ATTACACTTCTGCTAGGATAATCATTTCCATTGTCGAGGTGGGAACACAAAGGCTTGGAGTGGTTAATTC
CTTGCCAAGGCCCCGCAGGAGGAAGCAGGGTCCTGCATGGGAGGCCGGCTAGCCTGCCCACTGCCCCATG
TCCCCACAGGTGATGGAGGCCTTTGAGCAGGCCGAGCGGAAGCCCAAACCCAACCCCAACCTACTCTTCT
CAGACGTGTATCAGGAGATGCCCGCCCAGCTCCGCAAGCAGCAGGAGTCTCTGGCCCGCCACCTGCAGAC
CTACGGGGAGCACTACCCACTGGATCACTTCGATAAGTGAGACCTGCTCAGCCCACCCCCACCCATCCTC
AGCTACCCCGAGAGGTAGCCCCACTCTAAGGGGAGCAGGGGGACCTGACAGCACACCACTGTCTTCCCCA
GTCAGCTCCCTCTAAAATACTCAGCGGCCAGGGCGGCTGCCACTCTTCACCCCTGCTCCTCCCGGCTGTT
ACATTGTCAGGGGACAGCATCTGCAGCAGTTGCTGAGGCTCCGTCAGCCCCCTCTTCACCTGTTGTTACA
GTGCCTTCTCCCAGGGGCTGGGTGAGGGCACATTCAGGACTAGAAGCCCCTCTGGGCATGGGGTGGACAT
GGCAGGTCAGCCTGTGGAACTTGCGCAGGTGCGAGTGGCCAGCAGAGGTCACGAATAAACTGCATCTCTG
CGCCTGGCTCTCTACCACCTCTGGTCTTTGTTTCCTGGAGTTTGGGGGTGATTTGAGGCCACCCCCAGCT
TCTCCACCTTAGATAAATAAGGTCAAGGCTCTTGAGATACCTACTTCCCCCGGAAGCTTGCGCAAGCTGG
AAGGTTAACTTCAGCAGACAGGATGGGGGTGGGGGGGTCCTCCAGAGATGGTCTCCTGCTCTCTCACAGA
ATGGGAGAAGCCTGCTGTCCAGCAGAGTGATGGGGAGCCCAGTGGAGCTAGCCCTGCCCAGCAGGGGTGC
AGGGGAGCGTCCAGGATGAAGGTCAGGGTGTGGCCAAGCCCTGCTCCCCACCACTCCAGGGCCCCACAGA
TGCAAAAACAACCCCAGGCCTCAGAGTGGGGAGTGCATGTTTTTCAAAAACAAAAAGTAGGCAAAAACAG
TCCACCACCCCATCTTTCCTGCCCAGGCCCCTGGCTGGGGGAGGCCAAGGAGTGGCTTAAGTTGTCCAGC
TTCTGGGCCCTCCTCCCTCCCATCAAGACCTGGAAGGAGGGGCTGAGCCAGGGGCCGGCCCCAGAGGCCC
AGGCCAGGTCAGCACCTCCGCCCTCCCCAATGAGAGTCAGCACTGGAGCCTTGGGTCTTGCAGTTGTTTC
CAGAGCCGAATGCTGGCCTGGGGGCCCAGGGGCCGTACCAGCAGCAGGTTGCGGAAAGCACAGTGGTCCG
CAGTGCGGTCTGCTGGCCAGGCTGTGAGGAAGCCTGGGTGGGCCTGGGGCACCAGGCCCAGGGCTCGGAT
GCAAAGGCCAGTGTAGACGTCCTCAAAGGGGAAGGGTGCCACACGGGCTGCCGCCCGCAGCAGCCAGGGT
GCCAGGCGCCCGGCAATGACGTAGCCACCCCCGCTTGCATAGGCTGGGTAGCCACCTTCGAAGAAGGACT
CGGGCACATAGAAGGGTCCTCCTGGCTTCCGGAGAGGCATGGCCTGGGTAAAGACCTCACCCAGGTAGAG
GCTTCGGGCCGAGGCAGGTGGCAGGGCCCGCAGGTGAGCCAGCAGGGCAGGGGTGTGTACAAAGGCATCG
TCCTGAGCTCGCAAGACAAAACTCACGGTGGGGCAGTGGCGGCCCAGCCAGGCCAGCAGCAGCAGGTCTT
TGAGCGTCTGGTTGAATGGGACGTCGAGGAAGTCCCAGAGCAGCAGGTCACTGTAGCGACGGCTCTCCCA
GGCCACTAGTGAGTCTAGGTCAGGCCCCGCCTCACCCACCGGAGACCCTAGCAGGAAGAGCAGCCGGATC
CCTGGAGCTGGACTGCCCCACGTCTCTCTCACGGCCTGTCGTTCTGCAAAGCGCCCTGGTTCTGACTTGA
CGGCCAACAGCAGGTAGGGGACATCAGTATCTGAGCAGCTGGAGACTTGGCTGCCACCACCTCCAGGCAG
CCACTGTGGGAAGCTCCGGCAGGCTGCTGACAGCAAGAAGCGGCGGAGGTCCTTGGGGTAGGAGGCGAAG
TCAGGGATCTCGGTGGCGGCGGCGGCCCCCCAAGCCTGGCAGCCCCCCGTTTCAGTGCTGTCCCCACTGG
GCAGGGACCCCAGCCGCCACTGCTGCTGGTTCCAGTAAGCAAAGGGCAGCGGGATAGTCTGGCCCAGGCG
GGTGGAGAGGTTGGCAGGTAGGGTGGGCTCAGGGTTGGCTGGCGTGGGGCTTGGCGGGGTGCCCCGAGGG
CTGGGGTAGGCCTTGCTGAGCCGGGACTCGGATGTCCACTCGATGTACACTTTGAGGCCCAGGAGTGTGA
GCAGTGCTGACAGGCAGAGAAGGCACTTGGGGCAGCGCATGACCCGGCCCAGGGAAGGGGCGGCCGCGGG
AGCTACAAGGGAGCCACAGGGGAGCCACGGAGGCCATTGGGGTGTGGGGATGGGCCTCCAGAGAGGGCCC
AGCCAGGCCCCAGGCAGACAGCTTCACAATCTCCCATAGTCCCCGCCAACCCCCATAACAGATTCTCTCG
GTCCCAGATCCTAAACAGCTCCCCTGACCCAGGGACCTCGTTGGTCCTTATTCCTAG

mRNA transcript

>gi|258645170|ref|NM_000709.3| Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA
CTACGTGAGTGCCGGACCGCTGAGTGGTTGTTAGCCAAGATGGCGGTAGCGATCGCTGCAGCGAGGGTCT
GGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGCTGCTGCGGCAGCCTGGGGCTCGGGGACTGGC
TAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATCTCTGGATGACAAGCCCCAGTTCCCAGGGGCC
TCGGCGGAGTTTATAGATAAGTTGGAATTCATCCAGCCCAACGTCATCTCTGGAATCCCCATCTACCGCG
TCATGGACCGGCAAGGCCAGATCATCAACCCCAGCGAGGACCCCCACCTGCCGAAGGAGAAGGTGCTGAA
GCTCTACAAGAGCATGACACTGCTTAACACCATGGACCGCATCCTCTATGAGTCTCAGCGGCAGGGCCGG
ATCTCCTTCTACATGACCAACTATGGTGAGGAGGGCACGCACGTGGGGAGTGCCGCCGCCCTGGACAACA
CGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTGATGTATCGGGACTACCCCCTGGAACTATT
CATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAAGGGGCGCCAGATGCCTGTCCACTACGGCTGC
AAGGAACGCCACTTCGTCACTATCTCCTCTCCACTGGCCACGCAGATCCCTCAGGCGGTGGGGGCGGCGT
ACGCAGCCAAGCGGGCCAATGCCAACAGGGTCGTCATCTGTTACTTCGGCGAGGGGGCAGCCAGTGAGGG
GGACGCCCATGCCGGCTTCAACTTCGCTGCCACACTTGAGTGCCCCATCATCTTCTTCTGCCGGAACAAT
GGCTACGCCATCTCCACGCCCACCTCTGAGCAGTATCGCGGCGATGGCATTGCAGCACGAGGCCCCGGGT
ATGGCATCATGTCAATCCGCGTGGATGGTAATGATGTGTTTGCCGTATACAACGCCACAAAGGAGGCCCG
ACGGCGGGCTGTGGCAGAGAACCAGCCCTTCCTCATCGAGGCCATGACCTACAGGATCGGGCACCACAGC
ACCAGTGACGACAGTTCAGCGTACCGCTCGGTGGATGAGGTCAATTACTGGGATAAACAGGACCACCCCA
TCTCCCGGCTGCGGCACTATCTGCTGAGCCAAGGCTGGTGGGATGAGGAGCAGGAGAAGGCCTGGAGGAA
GCAGTCCCGCAGGAAGGTGATGGAGGCCTTTGAGCAGGCCGAGCGGAAGCCCAAACCCAACCCCAACCTA
CTCTTCTCAGACGTGTATCAGGAGATGCCCGCCCAGCTCCGCAAGCAGCAGGAGTCTCTGGCCCGCCACC
TGCAGACCTACGGGGAGCACTACCCACTGGATCACTTCGATAAGTGAGACCTGCTCAGCCCACCCCCACC
CATCCTCAGCTACCCCGAGAGGTAGCCCCACTCTAAGGGGAGCAGGGGGACCTGACAGCACACCACTGTC
TTCCCCAGTCAGCTCCCTCTAAAATACTCAGCGGCCAGGGCGGCTGCCACTCTTCACCCCTGCTCCTCCC
GGCTGTTACATTGTCAGGGGACAGCATCTGCAGCAGTTGCTGAGGCTCCGTCAGCCCCCTCTTCACCTGT
TGTTACAGTGCCTTCTCCCAGGGGCTGGGTGAGGGCACATTCAGGACTAGAAGCCCCTCTGGGCATGGGG
TGGACATGGCAGGTCAGCCTGTGGAACTTGCGCAGGTGCGAGTGGCCAGCAGAGGTCACGAATAAACTGC
ATCTCTGCGCCTGGCTCTCTACCAAAAAAAAAAAAAAAAAA

Protein

>gi|11386135|ref|NP_000700.1| 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor [Homo sapiens]
MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQP
NVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGT
HVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLA
TQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYR
GDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDE
VNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVYQEMPAQL
RKQQESLARHLQTYGEHYPLDHFDK

Neutral mutations

Disease causing mutations

Several disease causing mutations are reported in OMIM. Following table summarize the reported allelic variations:

Reported allelic variants in OMIM
ID Variant type Mutation detail Cause Severity
1 substitution TYR393ASN MSUD type 1A classic
2 deletion 8-BP DEL, 887-894 MSUD type 1A classic
3 substitution GLY245ARG MSUD type 1A intermediate
4 substitution PHE364CYS MSUD type 1A intermediate
5 substitution ARG220TRP MSUD type 1A classic
6 substitution GLY204SER MSUD type 1A classic
7 substitution THR265ARG MSUD type 1A classic
8 substitution CYS219TRP MSUD type 1A classic
9 deletion 1-BP DEL, 117C MSUD type 1A classic

References

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA)

RefSeqGene of branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA)

Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA

Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 2, mRNA

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial isoform 1 precursor (Homo sapiens)

Online Mendelian Inheritance in Man