Fabry:Structure-based mutation analysis

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Revision as of 20:53, 20 June 2012 by Staniewski (talk | contribs) (Preparation)

Preparation

<figtable id="tab:Prep"> All available PDB structures assigned to the Uniprot entry P06280 along with the according Resolution,
Coverage and R-factor. The R-factor was obtained from the PDBsum page. The chosen structure 3HG3 is highlighted.

Entry Method Resolution (Å) Chain Positions (up to 429) PDBsum PDB R-factor R-free
1R46 X-ray 3.25 A/B 32-422 ] ] 0.262 0.301
1R47 X-ray 3.45 A/B 32-422 ] ] 0.285 0.321
3GXN X-ray 3.01 A/B 32-421 ] ] 0.239 0.301
3GXP X-ray 2.20 A/B 32-422 ] ] 0.204 0.265
3GXT X-ray 2.70 A/B 32-422 ] ] 0.245 0.306
3HG2 X-ray 2.30 A/B 32-422 ] ] 0.178 0.202
3HG3 X-ray 1.90 A/B 32-426 ] ] 0.167 0.197
3HG4 X-ray 2.30 A/B 32-423 ] ] 0.166 0.221
3HG5 X-ray 2.30 A/B 32-422 ] ] 0.192 0.227
3LX9 X-ray 2.04 A/B 32-422 ] ] 0.178 0.218
3LXA X-ray 3.04 A/B 32-426 ] ] 0.216 0.244
3LXB X-ray 2.85 A/B 32-427 ] ] 0.227 0.264
3LXC X-ray 2.35 A/B 32-422 ] ] 0.186 0.237
3S5Y X-ray 2.10 A/B 32-422 ] ] 0.195 0.230
3S5Z X-ray 2.00 A/B 32-421 ] ] 0.211 0.234
3TV8 X-ray 2.64 A/B 32-422 ] ] 0.203 0.239

</figtable>

We chose to use the structure 3HG3, since it has the best resolution (1.90 Å) and the second best R-factor (see <xr id="tab:Prep"/>). The best R-factor, which is a measure of the agreement between the crystallographic model and the experimental X-ray diffraction data <ref>R-factor (crystallography) (May 17, 2012‎) http://en.wikipedia.org/wiki/R-factor_%28crystallography%29, June 20, 2012</ref>, could be achieved with the structure 3HG4, but it has a resolution of 2.30 Å and hence we decided to use 3HG3. All structures cover the protein completely except for the signal peptide from position 1 to 31.

Vizualisation

<figure id="fig:allSNPs">

All SNPs mapped onto the structure 3HG3. Mutated sites are shown in blue. The active site (residues 170 and 231) is shown in pink, substrate binding site (position 203-207) in cyan and the existing five disulfide bonds (52 ↔ 94, 56 ↔ 63, 142 ↔ 172, 202 ↔ 223, 378 ↔ 382) are highlighted in yellow

</figure>


Create mutation

SCWRL


Comparison energies

foldX

Minimise

Gromacs