CD task7 protocol
From Bioinformatikpedia
Choosing structure
So, we decided to use 2O4H, since it has a bound substrate, low resolution and a listed pH value.
Apo-structure: 2O53 Resolution: 2,7, R-free: 0,269, pH: 6.0, chains: A,B Holo-structure: 2O4H Resolution: 2,7, R-free: 0,271, pH: 6.0, chains: A,B intermediate substrate analog: N-phosphonomethyl-L-aspartate Apo-structure: 2I3C Resolution: 2,8, R-free: 0,243, pH: - , chains: A,B Ensemble Refinement 2Q51 Resolution: 2,8, R-free: 0,239, pH: - , chains: A,B
For 2O4H, there are only missing residues at the N- and C-terminal ends, which can be neglected:
REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 M RES C SSSEQI REMARK 465 1 ALA A -1 REMARK 465 1 ILE A 0 REMARK 465 1 ALA A 1 REMARK 465 1 THR A 2 REMARK 465 1 SER A 3 REMARK 465 1 CYS A 4 REMARK 465 1 HIS A 5 REMARK 465 1 ILE A 6 REMARK 465 1 ALA A 7 REMARK 465 1 GLU A 8 REMARK 465 1 CYS A 311 REMARK 465 1 LEU A 312 REMARK 465 1 HIS A 313
