Structure-based mutation analysis ARSA

Preparation

Visualization with Pymol

Pymol visualization of ARSA (with closed gaps). The active site is depicted in yellow, metal-binding site in blue, substrate binding sites in green and missense mutations in red.

Nr.	mutation	position	Pymol image	Description	Effect on function
1	Asp-Asn	29
2	Pro - Ala	136
3	Gln-His	153
4	Trp-Cys	193
5	Thr-Met	274
6	Phe -Val	356
7	Thr-Ile	409
8	Asn-Ser	440
9	Cys-Gly	489
10	Arg-His	496

BLA

repairPDB arsa_model.pdb -seq > arsa.model.seq
tr '[:upper:]' '[:lower:]' < arsa.model.seq > arsa.model.lower.seq