Structure based mutation analysis of GBA
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Contents
Introduction
Structure Selection
To carry out a structure-based analysis of the mutations chosen in Task 7 a crystal structure had to be chosen. According to Uniprot 19 different crystal structures of glucocerebrosidase exist. The table below shows the six different structures with a resolution of or better than 2 Angstrom. 2NT0 is chosen as template for the analysis carried out in this section, as no residues are missing, the R-value is quite low, and it has the best resolution among the structures without missing residues. Only incomplete structures have been resolved near the physiological pH (7.4), therefore a structure resolved at a more acid pH had to be chosen.
| PDB ID | Resolution [Å] | R-factor | Coverage | pH | Missing Residues (A/B) |
|---|---|---|---|---|---|
| 1OGS | 2.00 | 0.195 | 4.6 | 0 | |
| 2NT0 | 1.79 | 0.181 | . | 4.5 | 0 |
| 2V3D | 1.96 | 0.157 | 6.5 | 9/8 | |
| 2V3E | 2.00 | 0.163 | 7.5 | 7/7 | |
| 2V3F | 1.95 | 0.154 | 6.5 | 8/14 | |
| 3GXI | 1.84 | 0.193 | NULL | 0 |
Mutations
TODO: create image and so on ...
