Structure-based mutation analysis GLA

^{by Benjamin Drexler and Fabian Grandke}

Introduction

In this task we analyse the structure of our protein to find out what effects the point mutations have. Therefor we create a mutated structure and compare it to the wild-type protein.

Structure Selection

There are several structure files available for our protein:

PDB ID	Resolution [Å]	ph-Value	R-Factor	Coverage [%]
1R46	3.25	8.0	0.262	99.7
1R47	3.45	8.0	0.285	99.5
3GXN	3.01	NULL	0.239	88.08
3GXP	2.20	NULL	0.204	81.9
3GXT	2.70	NULL	0.245	97.29
3GH2	2.30	4.6	0.178	97.32
3GH3	1.90	6.5	0.167	98.64
3GH4	2.30	4.6	0.166	99.86
3GH5	2.30	4.6	0.192	100
3LX9	2.04	6.5	0.178	98.92
3LXA	3.04	6.5	0.216	99.52
3LXB	2.85	6.5	0.227	99.3
3LXC	2.35	6.5	0.186	98.31

We set certain cutoffs to decide which structures are excluded:

• ph-value: < 6.5
• resolution: > 2.7

After we applied the cutoffs to our set of structures three were left (exclusion factors are colored red in the table). One of them was slightly better than the other ones so we decided to use 3HG3 (worse values are colored gray in the table). Additionally 3GH3 has the best resolution and R-factor (colored green).

References

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