Fabry:Sequence-based analyses
The following analyses were performed on the basis of the α-Galactosidase A sequence.
Contents
Secondary structure
Disorder
Transmembrane helices
Signal peptides
GO terms
GOPET
Searching the GOPET annotation tool with the AGAL_HUMAN sequence revealed 5 GOIds, which are displayed in <xr id="Tab:GOPET"/>. On a first glance, since we already know the name and function of the protein, it is a bit surprising, that alpha-galactosidase activity is only the third entry with 96% confidence. In our already carried out information gathering we learned that α-galactosidase A is a hydrolase thus the first three entries were not surprising. Considering that our enzyme mainly is a glycosidase, the both entries on top of the list make perfekt sense.
Again a bit surprising was the last entry. α-N-Acetylgalactosaminidase is actually used for enzyme replacement therapy, which we mention on our main page. The structure of both enzymes is similar to each other, but this still does not explain the association of this GO term to the AGAL protein.
Result for GOPET search | |||||
---|---|---|---|---|---|
GOid | Aspect | Confidence | GO term | ||
GO:0016798 | Molecular Function Ontology (F) | 98% | hydrolase activity acting on glycosyl bonds | ||
GO:0004553 | Molecular Function Ontology (F) | 98% | hydrolase activity hydrolyzing O-glycosyl compounds | ||
GO:0016787 | Molecular Function Ontology (F) | 97% | hydrolase activity | ||
GO:0004557 | Molecular Function Ontology (F) | 96% | alpha-galactosidase activity | ||
GO:0008456 | Molecular Function Ontology (F) | 89% | alpha-N-acetylgalactosaminidase activity |