Structure-based mutation analysis (PKU)
From Bioinformatikpedia
short task description
Finding the right structure
As proposed we searched the UNIProt entry for our protein and then selected the entry with the highest resolution and the lowest r-Value. In our case this is 1J8U which is the protein in a complex with its cosubstrate BH4. IN the following we will only use this structure, but we also list the results we found. <figtable id="uniprotresult">
| Entry | Method | Resolution (Å) | r-Value | Chain | Positions | PDBsum |
|---|---|---|---|---|---|---|
| 1DMW | X-ray | 2.00 | 0.200 | A | 118-424 | [»] |
| 1J8T | X-ray | 1.70 | 0.197 | A | 103-427 | [»] |
| 1J8U | X-ray | 1.50 | 0.157 | A | 103-427 | [»] |
| 1KW0 | X-ray | 2.50 | 0.220 | A | 103-427 | [»] |
| 1LRM | X-ray | 2.10 | 0.211 | A | 103-427 | [»] |
| 1MMK | X-ray | 2.00 | 0.199 | A | 103-427 | [»] |
| 1MMT | X-ray | 2.00 | 0.213 | A | 103-427 | [»] |
| 1PAH | X-ray | 2.00 | 0.176 | A | 117-424 | [»] |
| 1TDW | X-ray | 2.10 | 0.206 | A | 117-424 | [»] |
| 1TG2 | X-ray | 2.20 | 0.213 | A | 117-424 | [»] |
| 2PAH | X-ray | 3.10 | 0.251 | A/B | 118-452 | [»] |
| 3PAH | X-ray | 2.00 | 0.175 | A | 117-424 | [»] |
| 4ANP | X-ray | 2.11 | 0.204 | A | 104-427 | [»] |
| 4PAH | X-ray | 2.00 | 0.169 | A | 117-424 | [»] |
| 5PAH | X-ray | 2.10 | 0.163 | A | 117-424 | [»] |
| 6PAH | X-ray | 2.15 | 0.171 | A | 117-424 | [»] |
</figtable> In <xr id="uniprotresult"/> there are all results according to which we selected 1J8U to be our reference for this weeks task. The corresponding line is marked in yellow.
