Researching SNPs Gaucher Disease
The aim of this task was to map non-synonymous SNPs known for the Gaucher Disease causing gene GBA onto the corresponding protein sequence P04062. Several databases should be taken into account, namely HGMD<ref name="hgmd">Stenson et al. (2009). The Human Gene Mutation Database (HGMD): 2008 Update. Genome Med</ref>, dbSNP<ref name="dbsnp">Sherry ST, Ward M, and Sirotkin, K. (1999). dbSNP - database for single nucleotide polymorphisms and other classes of minor genetic variation. Genome Research</ref>, SNPdb <ref name="snpdbe">C Schaefer, A Meier, B Rost. (2012). SNPdbe: constructing an nsSNP functional impacts database. Bioinformatics</ref>, and OMIN <ref name="omim">Hamosh, A.; Scott, A.; Amberger, J.; Bocchini, C.; McKusick, V. (2004). Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders. Nucleic Acids Research</ref>. Technical details are reported in our protocol.
HGMD
The Human Genome Mutation Database (HGMD) is a collection of observed mutations in the human genome which are associated with various diseases. The mutations are taken from publications, both manually and automatically [1]. There is a public version with 88745 entries (9th June, 2012) and a professional version with 123565 entries. Since 2009, the public version has been extended only by few new entries such that it does no longer contain all currently known mutations [2]. The HGMD encompasses following types of mutations:
- Missense
- Mutations which result in another amino acid
- Nonsense
- Mutations which result in a stop codon
- Splicing
- Mutations which alter splice sites
- Regulatory
- Mutation which alter gene regulation
- Small/gross deletions
- Deletions which remove residues
- Small/gross insertions
- Insertions which give rise to new residues
- Small indels
- Insertions or deletions
- Duplications
- Duplication of gene fragments
- Complex rearrangements
- Change in the location for gene fragments
- Repeat variations
- Varying number of sequence copies
<xr id="tab:hgmd_number"/> lists the number of mutations annotated in HGMD for GBA. A comprehensive list of all missense/nonsense mutations can be obtained by following the respective hyperlink.
<figtable id="tab:hgmd_number">
| Type | Count |
|---|---|
| Missense/nonsense | 250 |
| Splicing | 15 |
| Regulatory | 0 |
| Small deletions | 26 |
| Small insertions | 13 |
| Small indels | 2 |
| Gross deletions | 3 |
| Gross insertions/duplications | 1 |
| Complex rearrangements | 15 |
| Public total | 325 |
Number of mutations in HGMD for GBA. </figtable>
<xr id="tab:hgmd_map"/> shows the wildtype version of P04062 with non-synonymous mutations highlighted in red. Since the transcript NM_001005741.1 in HGMD already referred to P04062, the amino acid positions could be used directly for creating <xr id="tab:hgmd_map"/> without any additional translation.
<figtable id="tab:hgmd_map">
1 11 21 31 41 51 61 71
Wildtype MEFSSPSREE CPKPLSRVSI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY SSVVCVCNAT YCDSFDPPTF PALGTFSRYE
Non-synonymous MEFSSPSREE CPRPLSRVSI MAGSLTGLLL LQAVS!ASGA RPCIPKSFSY ISVLSVCNAT YCNSFDPPTF PALSTVSRYK
81 91 101 111 121 131 141 151
Wildtype STRSGRRMEL SMGPIQANHT GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE EGIGYNIIRV
Non-synonymous NIRSE!QMEL SMGPIQANHT GTGLPLTLQP E!!FQKANGF GGATTDAATL NILALSPPAQ NLLRKLCVSK EEIGYDISQA
161 171 181 191 201 211 221 231
Wildtype PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI HRALQLAQRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP
Non-synonymous LVASCVFSIL TYP!EDTPHD FQLHNFSLPE ADTKLQILLS PQALQLA!CP V!PLDSS!PS LTRFKTKVEG NGKWPPEGQP
241 251 261 271 281 291 301 311
Wildtype GDIYHQTWAR YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA RDLGPTLANS THHNVRLLML
Non-synonymous EDICHQTRVR HCVKYLDACA EHKLQFWAVR A!NETPAVLL SVHHFQCQGL TPEQQ!DLTA HDLDATLANG THHNVRLPML
321 331 341 351 361 371 381 391
Wildtype DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
Non-synonymous DDQHLLPLHW AKVVLTDPEA AI!LHGIVVR RHLHFLDSAK AIPWKTHCLS PNTMPFASET YVGSKFGK!S LWLDF!D!GI
401 411 421 431 441 451 461 471
Wildtype QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASQK
Non-synonymous QCRHGIIMKV LYHLVS!TA! KRDPNL!ERL IRLCTSFYSL FIVDITKHTI HQRRVVCHLD HFSEVIPEGS QGVGLVASQK
481 491 501 511 521 531
Wildtype NDLDAVALMH PDGSAVVVVL NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ
Non-synonymous NDRDPVALMR PDGSPVVVMP KPSSKDVPLT IKYPAVSFPE TISPGYPTHI YLWRCR
Genetic variants of P04062 annotated in HGMD. Red: non-synonymous mutations;
</figtable>
dbSNP
dbSNP is a publicly available collection of genetic variations from different species. Genetic variations include (1) SNPs, (2) short deletion and insertion polymorphisms (indels/DIPs), (3) microsatellite markers or short tandem repeats (STRs), (4) multinucleotide polymorphisms (MNPs), (5) heterozygous sequences, and (6) named variants. Genetic variants are submitted by, amongst others, research institutions, large scale genome sequencing centers, and other SNP databases. Statistics about the latest release can be found here.
Variations of GBA in dbSNP included 92 missense mutations and 47 synonymous mutations. These mutations are visualized in <xr id="tab:dbsnp_map"/>.
<figtable id="tab:dbsnp_map">
1 11 21 31 41 51 61 71
Wildtype MEFSSPSREE CPKPLSRVSI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY SSVVCVCNAT YCDSFDPPTF PALGTFSRYE
Non-synonymous MEFSSPSREE CPRPSGGVSV MAGSLTGLLL LQAVSWASGA CPCIPESFGY SSVVCVCNAT YYESFDPRTF PALGTLSCYK
Synonymous MEFSSPSREE CPKPLSRVSI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY SSVVCVCNAT YCDSFDPPTF PALGTFSRYE
81 91 101 111 121 131 141 151
Wildtype STRSGRRMEL SMGPIQANHT GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE EGIGYNIIRV
Non-synonymous SRSSGQWMEL STGPIQANYT GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE EGIGYKISWV
Synonymous STRSGRRMEL SMGPIQANHT GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE EGIGYNIIRV
161 171 181 191 201 211 221 231
Wildtype PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI HRALQLAQRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP
Non-synonymous LIASCVFSIL TYIYEDTPHD FQLHNFSLPE EDTKLNILLN PRALQLAQRP ISLLASPWTS LTRLKTKVEE NGKESLKGQP
Synonymous PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI HRALQLAQRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP
241 251 261 271 281 291 301 311
Wildtype GDIYHQTWAR YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA RDLGPTLANS THHNVRLLML
Non-synonymous EDICHQTWAR YLVKFLDAYA EHKLQFWAVR AENEPSAGLL NGYPFQCLGF TPEHQRDFIA HDLDRTLANG THHNVRLLML
Synonymous GDIYHQTWAR YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA RDLGPTLANS THHNVRLLML
321 331 341 351 361 371 381 391
Wildtype DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
Non-synonymous DDQHLLLPQW AKVVLTDPEA AICVYGIAVH WYLDFLDPAK ATLGETHHLF PNTMLFASEA CVGSKFWEQS VQLGSWDQGI
Synonymous DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
401 411 421 431 441 451 461 471
Wildtype QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASQK
Non-synonymous QCSHRIIMNL LYHVVGCTAW NIALNPKGGL IWVRTSVESP TIVDITKETL YKQPILCHLG HFSKFIPEGS QRVGLVASPK
Synonymous QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASQK
481 491 501 511 521 531
Wildtype NDLDAVALMH PDGSAVVVVL NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ
Non-synonymous NNLDAVALMR PDGSAVVVVL NRSFKDVPPT IKEPAVGFLE TISPGYSIHI YLWCHQ
Synonymous NDLDAVALMH PDGSAVVVVL NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ
Genetic variants of P04062 annotated in dbSNP. Red: missense mutations; Green: synonymous mutations; Purple: missense and synonymous mutations.
</figtable>
References
<references/>
