Task 3: odba human Sequence-based predictions

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Revision as of 14:36, 12 May 2012 by Kiening (talk | contribs) (reprof)

secondary structure

To predict secondary structure we use the following tools and compare the results:

-reprof
-psipred
-DSSP_Server

reprof

to run reprof from the command line the following command is used:

reprof -i seq.fasta

reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof" Result:

MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPR
LLHHHHHHHHHHHHLLLLHHHHHHHLLLLLLLELLLLLLL
QQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYR
LHLLLLLLLLLLLLLLLLHHHHHHHHLLLLLLELLLLEEE
VMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILY
EELLLLLEELLLLLLLLLHHHHHHHHHHHHHHHLHLHHEE
ESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYRE
ELLLLLEEEEEEELLLLLLLLELLLLELLLLLEEEEEELL
AGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKER
LLEEEELLLLHHHHHHHHHLLHLLLLLLLLLLLELLLLLL
HFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGA
EEEEELLLHHHHLHHHHHHHHHHHHLLLLEEEEEEELLLL
ASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYR
LLLLLLLLLLEEEEELLLLEEEEEELLLEEELLLLLLLEL
GDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAE
LLLEELLLLLEEEEEEEELLLLEEEEELLLHHHHHHHHHL
NQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHP
LLLEEEEHEEEEELLLLLLLLLLHLLLLLLLLLLLLLLLL
ISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERK
HHHHHHHHHLLLLLLHHHHHHHHHHHHHHHHHHHHHHHLL
PKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPL
LLLLLLEEELHHHHHLHHHHHHHHHHHHHHHHHHLLLLLL
DHFDK

Disorder

Transmembrane helices

Signal peptides

GO terms