Difference between revisions of "Structure-based mutation analysis ARSA"
From Bioinformatikpedia
(→Visualization with Pymol) |
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| [[File:arsa356.png | 200px | center | thumb ]] |
| [[File:arsa356.png | 200px | center | thumb ]] |
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| + | | The mutation is at moderate distance to all important functional sites of the protein. |
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| + | | neutral |
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| 7 |
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| 409 |
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| [[File:arsa409.png | 200px | center | thumb ]] |
| [[File:arsa409.png | 200px | center | thumb ]] |
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| + | | The mutation is not close to important functional sites. |
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| + | | harmful |
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| 8 |
| 8 |
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| 440 |
| 440 |
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| [[File:arsa440.png | 200px | center | thumb ]] |
| [[File:arsa440.png | 200px | center | thumb ]] |
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| + | | The mutation is very distant from all functional sites. |
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| + | | neutral |
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| 9 |
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| 489 |
| 489 |
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| [[File:arsa489.png | 200px | center | thumb ]] |
| [[File:arsa489.png | 200px | center | thumb ]] |
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| + | | The mutation is very distant from all functional sites. |
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| + | | harmful |
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| 10 |
| 10 |
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| 496 |
| 496 |
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| [[File:arsa496.png | 200px | center | thumb ]] |
| [[File:arsa496.png | 200px | center | thumb ]] |
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| + | | The mutation is very distant from all functional sites. |
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| + | | harmful |
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Revision as of 09:28, 30 June 2011
Preparation
Visualization with Pymol
| Nr. | mutation | position | Pymol image | Description | Effect on function |
| 1 | Asp-Asn | 29 | The mutation is located at the position of a metal-binding site. | harmful | |
| 2 | Pro - Ala | 136 | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. | harmful | |
| 3 | Gln-His | 153 | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. | harmful | |
| 4 | Trp-Cys | 193 | The mutation is at moderate distance to all important functional sites of the protein. | neutral | |
| 5 | Thr-Met | 274 | The mutation is located very distant from the active site and a substrate binding site in sequence as well as in structure. It is located within a beta sheet. |
harmful | |
| 6 | Phe -Val | 356 | The mutation is at moderate distance to all important functional sites of the protein. | neutral | |
| 7 | Thr-Ile | 409 | The mutation is not close to important functional sites. | harmful | |
| 8 | Asn-Ser | 440 | The mutation is very distant from all functional sites. | neutral | |
| 9 | Cys-Gly | 489 | The mutation is very distant from all functional sites. | harmful | |
| 10 | Arg-His | 496 | The mutation is very distant from all functional sites. | harmful |
SCRWL
BLA
repairPDB arsa_model.pdb -seq > arsa.model.seq
tr '[:upper:]' '[:lower:]' < arsa.model.seq > arsa.model.lower.seq
