Difference between revisions of "Structure-based mutation analysis ARSA"
From Bioinformatikpedia
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=== Preparation === |
=== Preparation === |
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| [[File:arsa29.png | 200px | center | thumb ]] |
| [[File:arsa29.png | 200px | center | thumb ]] |
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| + | | The mutation is located at the position of a metal-binding site. |
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| + | | harmful |
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| 2 |
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| 136 |
| 136 |
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| [[File:arsa136.png | 200px | center | thumb ]] |
| [[File:arsa136.png | 200px | center | thumb ]] |
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| + | | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. |
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| + | | harmful |
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| 153 |
| 153 |
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| [[File:arsa153.png | 200px | center | thumb ]] |
| [[File:arsa153.png | 200px | center | thumb ]] |
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| + | | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. |
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| + | | harmful |
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| 4 |
| 4 |
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| 193 |
| 193 |
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| [[File:arsa193.png | 200px | center | thumb ]] |
| [[File:arsa193.png | 200px | center | thumb ]] |
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| + | | The mutation is at moderate distance to all important functional sites of the protein. |
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| + | | neutral |
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| 274 |
| 274 |
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| [[File:arsa274.png | 200px | center | thumb ]] |
| [[File:arsa274.png | 200px | center | thumb ]] |
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| + | | The mutation is located very distant from the active site and a substrate binding site in sequence as well as in structure. <br> It is located within a beta sheet. |
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| + | | harmful |
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Revision as of 08:24, 30 June 2011
Preparation
Visualization with Pymol
| Nr. | mutation | position | Pymol image | Description | Effect on function |
| 1 | Asp-Asn | 29 | The mutation is located at the position of a metal-binding site. | harmful | |
| 2 | Pro - Ala | 136 | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. | harmful | |
| 3 | Gln-His | 153 | The mutation is located near the active site and a substrate binding site in sequence as well as in structure. | harmful | |
| 4 | Trp-Cys | 193 | The mutation is at moderate distance to all important functional sites of the protein. | neutral | |
| 5 | Thr-Met | 274 | The mutation is located very distant from the active site and a substrate binding site in sequence as well as in structure. It is located within a beta sheet. |
harmful | |
| 6 | Phe -Val | 356 | |||
| 7 | Thr-Ile | 409 | |||
| 8 | Asn-Ser | 440 | |||
| 9 | Cys-Gly | 489 | |||
| 10 | Arg-His | 496 |
SCRWL
BLA
repairPDB arsa_model.pdb -seq > arsa.model.seq
tr '[:upper:]' '[:lower:]' < arsa.model.seq > arsa.model.lower.seq
