Difference between revisions of "Sequence-based mutation analysis BCKDHA"

Revision as of 18:40, 21 June 2011

	Reference amino acid			Mutated amino acid			BLOSUM62	PAM1	PAM250	Secondary Structure
Position	Residue	Properties	Structure	Residue	Properties	Structure	BLOSUM62	PAM1	PAM250	Secondary Structure
29	G	tiny, small		E	charged, polar				C
125	Q	acidic, polar		E	charged, polar				C
166	Y	hydrophobic, aromatic, polar		N	acidic, polar, small				C
249	G	tiny, small		polar, small, tiny, hydroxylic				C
264	C	sulphur containing, hydrophobic, tiny, small, polar		W	hydrophobic, aromatic, polar				C
265	R	charged, positive (basic), polar		W					C
326	I			T					C
361	I			V					C
409	F			C					C
438	Y			N					C

PsiBlast was run using the following command: blastpgp -i sequence.fasta -j 5 -d /data/blast/nr/nr -C profile.ckp

@@ Line 9: / Line 9: @@
 !rowspan="2"|PAM1
 !rowspan="2"|PAM250
+!rowspan="2"|Secondary Structure
 |-
 !Position
@@ Line 18: / Line 19: @@
 !Structure
 |-
-|29||G||tiny, small||||E||charged, polar||
+|29||G||tiny, small||||E||charged, polar|| || || ||C
 |-
-|125||Q||acidic, polar||||E||charged, polar||
+|125||Q||acidic, polar||||E||charged, polar|| || || ||C
 |-
-|166||Y||hydrophobic, aromatic, polar||||N||acidic, polar, small||
+|166||Y||hydrophobic, aromatic, polar||||N||acidic, polar, small|| || || ||C
 |-
-|249||G||tiny, small||||S|polar, small, tiny, hydroxylic|||
+|249||G||tiny, small||||S|polar, small, tiny, hydroxylic|| || || ||C
 |-
-|264||C||sulphur containing, hydrophobic, tiny, small, polar||||W||hydrophobic, aromatic, polar||
+|264||C||sulphur containing, hydrophobic, tiny, small, polar||||W||hydrophobic, aromatic, polar|| || || ||C
 |-
-|265||R||charged, positive (basic), polar||||W||||
+|265||R||charged, positive (basic), polar||||W|||| || || ||C
 |-
-|326||I||||||T||||
+|326||I||||||T|||| || || ||C
 |-
-|361||I||||||V||||
+|361||I||||||V|||| || || ||C
 |-
-|409||F||||||C||||
+|409||F||||||C|||| || || ||C
 |-
-|438||Y||||||N||||
+|438||Y||||||N|||| || || ||C
 |}