Difference between revisions of "Glucocerebrosidase homology modelling"
(→SWISS-MODEL) |
(→Homologous Structures) |
||
| Line 7: | Line 7: | ||
|colspan="5" |''' > 60% sequence identity ''' |
|colspan="5" |''' > 60% sequence identity ''' |
||
|- |
|- |
||
| − | | '''PDB-ID''' || '''name''' || ''' |
+ | | '''PDB-ID''' || '''name''' || '''organism''' || '''identity'''|| '''template ''' |
|- |
|- |
||
| 2nt0 || Glucosylceramidase || Homo Sapiens || 99% || |
| 2nt0 || Glucosylceramidase || Homo Sapiens || 99% || |
||
| Line 15: | Line 15: | ||
|colspan="5" |''' > 40% sequence identity ''' |
|colspan="5" |''' > 40% sequence identity ''' |
||
|- |
|- |
||
| − | | '''PDB-ID''' || '''name''' || ''' |
+ | | '''PDB-ID''' || '''name''' || '''organism''' || '''identity'''|| '''template ''' |
|- |
|- |
||
| || || || || |
| || || || || |
||
| Line 21: | Line 21: | ||
|colspan="5" |''' > 0% sequence identity''' |
|colspan="5" |''' > 0% sequence identity''' |
||
|- |
|- |
||
| − | | '''PDB-ID''' || '''name''' || ''' |
+ | | '''PDB-ID''' || '''name''' || '''organism''' || '''identity'''|| '''template ''' |
|- |
|- |
||
| 2wnw || SrfJ || Salmonella enterica subsp. enterica ||29% || x |
| 2wnw || SrfJ || Salmonella enterica subsp. enterica ||29% || x |
||
Revision as of 17:23, 8 June 2011
Contents
Homologous Structures
The 10 best results of the sequence search with HHSearch (as retrieved in Task 1) are listed in the table below.
| > 60% sequence identity | ||||
| PDB-ID | name | organism | identity | template |
| 2nt0 | Glucosylceramidase | Homo Sapiens | 99% | |
| > 40% sequence identity | ||||
| PDB-ID | name | organism | identity | template |
| > 0% sequence identity | ||||
| PDB-ID | name | organism | identity | template |
| 2wnw | SrfJ | Salmonella enterica subsp. enterica | 29% | x |
| 3clw | conserved exported protein | Bacteroides fragilis | 13% | |
| 3kl0 | Glucuronoxylan Xylanohydrolase | Bacillus subtilis | 18% | x |
| 1nof | xylanase | Erwinia chrysanthemi | 18% | |
| 2e4t | Endoglucanase | Clostridium thermocellum | 11% | x |
| 3ii1 | Cellulase | Uncultured bacterium | 15% | |
| 1qw9 | Arabinosidase | Geobacillus stearothermophilus | 13% | |
| 1ik2 | Endoglucanase | Clostridium acetobutylicum | 12 % | |
| 2c7f | alpha-L-Arabinofuranosidase | Clostridium thermocellum | 16% | |
Calculation of Models
MODELLER
iTasser
SWISS-MODEL
SWISS-MODEL workspace was published by Arnold et al. in 2005. <ref> Arnold K., Bordoli L., Kopp J., and Schwede T. (2006). The SWISS-MODEL Workspace: A web-based environment for protein structure homology modelling. Bioinformatics, 22,195-201.</ref>
Usage - Automated Mode
- Webserver: http://swissmodel.expasy.org/workspace/index.php?func=modelling_simple1
- Input: Sequence in fasta format
The automated mode should only be used, if target and template share more than 50% of sequence identity. This is not the case for the proteins selected as target in this case. Therefore the Alignment Mode will be used to create the models for glucocerebrosidase.
Usage - Alignment Mode
- Webserver: http://swissmodel.expasy.org/workspace/index.php?func=modelling_align1
- Input: Target-Template Alignment in different formats (FASTA, CLUSTALW, ...)
To create the Alignments needed as input, the tool ClustalW was used.
2WNW
Using the standard output alignment of ClustalW, the workunit of Swiss-Model got aborted: too many unfruitful attempts to rebuild a loop were tried. This indicates, that the alignment is not good and that it has to be adjusted.
References
<references />
