Difference between revisions of "Lab journal task 8"
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== Mutation analysis == |
== Mutation analysis == |
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| − | The description of the physicochemical properties is based on the wiki entry for amino acids [http://en.wikipedia.org/wiki/Amino_acid |
+ | The description of the physicochemical properties is based on the wiki entry for amino acids [http://en.wikipedia.org/wiki/Amino_acid wikipedia]. |
The mutations were visualized with Pymol. Because the pdb structure 1A6Z starts at position 22 in the reference structure, we subtracted 22 from the codon position to get the position of the mutation in the structure. The mutatins were done following the description in [http://www.pymolwiki.org/index.php/Mutagenesis Use PyMOL for this]. |
The mutations were visualized with Pymol. Because the pdb structure 1A6Z starts at position 22 in the reference structure, we subtracted 22 from the codon position to get the position of the mutation in the structure. The mutatins were done following the description in [http://www.pymolwiki.org/index.php/Mutagenesis Use PyMOL for this]. |
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Revision as of 18:41, 22 August 2013
Mutation selection
10 mutations were randomly selected from HGMD and dbSNP.
Mutation analysis
The description of the physicochemical properties is based on the wiki entry for amino acids wikipedia.
The mutations were visualized with Pymol. Because the pdb structure 1A6Z starts at position 22 in the reference structure, we subtracted 22 from the codon position to get the position of the mutation in the structure. The mutatins were done following the description in Use PyMOL for this. We did mutations for the first 9 SNPs but the last one (Arg330Met) could not be visualized, because the pdb structure is shorter than the reference sequence and only contains the residues 22 to 297. The rotamer for each mutated residue was selected based on the orientation and the size and color of the discs. We selected the rotamers with the least and smallest red discs if there was none without. For residues that are located on the border of the protein, we also tried to find rotamers that are not pointed into the solvent.
