Multiple Alignment Mammalian PheOH

We identified 22 mammalian homologs of phenylalanine hydroxylase using a simple blast search in Uniprot restricted to the Mammals database and filtered duplicates and protein fragments by hand. The found sequences are from the following organisms:

• Bos taurus (Q2KIH7)
• Callithrix jacchus (F7I717)
• Canis familiaris (E2R366)
• Cavia porcellus (H0VKI3)
• Cricetulus griseus (G3H671)
• Equus caballus (F7BKF9)
• Gorilla gorilla gorilla (G3QF53)
• Heterocephalus glaber (G5AMD7)
• Homo sapiens (P00439)
• Loxodonta africana (G3TIW0)
• Macaca fascicularis (G7PJC2)
• Macaca mulatta (F7HMW9)
• Monodelphis domestica (F7G2Z2)
• Mus musculus (P16331)
• Myotis lucifugus (G1P4I7)
• Nomascus leucogenys (G1R3M2)
• Ornithorhynchus anatinus ()
• Oryctolagus cuniculus (G1T8B6)
• Otolemur garnettii (H0WTI6)
• Rattus norvegicus (P04176)
• Sarcophilus harrisii (G3WCK3)
• Sus scrofa (F1SRJ9)

The alignment was done with T-Coffee on standard settings and, as all sequences are quite closely related, the alignment does not present much of a challenge for T-Coffee. All the regions around our investigated SNPs are -- judging by appearance -- optimally aligned.

All but two mutations are prefectly conserved. Of these two, at Ser87 appear one threonine and one asparagine. The threonine is an apparently similar replacement, the asparagine seems less conservative. Gly337 is in two cases replaced by glutamic acid. Additionally, the immediate neighbourhood around Ser87 is not perfectly conserved, the neighbourhood around Gly337 better, but also not perfectly conserved. The regions around Glu76 and Arg158 also sport some variance, whereas all the other SNP sites appear highly conserved. In summary, from this alignment we can infer some variability for the Glu76, Ser87, Arg158 and Gly337 site and high conservation for all the others.

sp|P00439|PH4H_HUMAN    MSTAVLENP-GLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALA
tr|G3QF53|G3QF53_GORGO  MSTAVLENP-GLGRKLSDFEQETSYIEDNCNQNGAISLIFSLKEEVGALA
tr|G1R3M2|G1R3M2_NOMLE  MSTAVLENP-GLGRKLSDFGQETSYVEDNSNQNGAISLIFSLKEEVGALA
tr|G7PJC2|G7PJC2_MACFA  MSTAVLENP-GLVRKLSDFGQETSYIEDNANQNGAISLIFSLKEEVGALA
tr|F7HMW9|F7HMW9_MACMU  MSTAVLENP-GLVRKLSDFGQETSYIEDNANQNGAISLIFSLKEEVGALA
tr|F7I717|F7I717_CALJA  MSTAVLENR-GFGRKLSDLGQETSYIEDNSTQNGAISLIFSLKEEVGALA
tr|F7BKF9|F7BKF9_HORSE  MSAVVLENR-GFGRKFSDFGQETSYIEDNSNPNGAISLIFSLKEEVGALA
tr|G1T8B6|G1T8B6_RABIT  MSAVVLENG-GLSRKFSDFGQETSYIEDNSNENSAISLIFSLKEEVGALA
tr|E2R366|E2R366_CANFA  MSAEVLEHR-GSGSKLSD-GQETSYIEDNSNQNGAISLIFSLKEEIGALA
tr|H0WTI6|H0WTI6_OTOGA  MTAVVLENR-LVGRKLSDSGQETSYIEENSNQSGAISLIFSLKEEVGALA
tr|G3TIW0|G3TIW0_LOXAF  MAAVVLESQ-GLGKKFSDFGQETSYTEDNSNQNGDISLIFSLKEEVGALA
sp|Q2KIH7|PH4H_BOVIN    MSALVLESR-ALGRKLSDFGQETSYIEGNSDQN-AVSLIFSLKEEVGALA
sp|P16331|PH4H_MOUSE    MAAVVLENG-VLSRKLSDFGQETSYIEDNSNQNGAVSLIFSLKEEVGALA
tr|G1P4I7|G1P4I7_MYOLU  MSAVVLENRRGLTRKLSDFGQETSYIENNSNENDAVSLIFSLKEEVGALA
sp|P04176|PH4H_RAT      MAAVVLENG-VLSRKLSDFGQETSYIEDNSNQNGAISLIFSLKEEVGALA
tr|H0VKI3|H0VKI3_CAVPO  MSAVVVEGR-GFGRKFSDFGQETSYIEDNSSQHSAISLIFSLKEEVGALA
tr|G3H671|G3H671_CRIGR  MSAVVLENP-VLTRKLSDFGQ-----------------------------
tr|G5AMD7|G5AMD7_HETGA  MSAVVLENR-GLDRKFSDFGQETRYIEDNSSQNSAISLIFSLKEEVGALA
tr|F7G2Z2|F7G2Z2_MONDO  ----------------------TSYIGDHTNQNGAISLIFSLKEEVGALA
tr|F6QV07|F6QV07_ORNAN  SSPTQVKPT-FVGQNLTPRGMETSYIEDNSNQNGAISLIFSLKEEVGALA
tr|G3WCK3|G3WCK3_SARHA  MSTASVGCI-GFERRS----SETSYIGDNTNQNGAISLIFSLKEEVGALA
tr|F1SRJ9|F1SRJ9_PIG    --------------------------------------------------
                                                                          

sp|P00439|PH4H_HUMAN    KVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILR
tr|G3QF53|G3QF53_GORGO  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILR
tr|G1R3M2|G1R3M2_NOMLE  KVLHLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILR
tr|G7PJC2|G7PJC2_MACFA  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILR
tr|F7HMW9|F7HMW9_MACMU  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILR
tr|F7I717|F7I717_CALJA  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILK
tr|F7BKF9|F7BKF9_HORSE  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTYLDKRSMPVLANIIKILR
tr|G1T8B6|G1T8B6_RABIT  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTYLDKRSMPALEDIIQILR
tr|E2R366|E2R366_CANFA  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTYLDKRSMPVLGNIIKILR
tr|H0WTI6|H0WTI6_OTOGA  KVLRLFEVNDINLTHIESRPSRLKKDEYEFFTYMDKRSAPSLENIIRILR
tr|G3TIW0|G3TIW0_LOXAF  KVLRLFEENDINLTHIESRPSRLKKDEYEFFTHLDKRSMPALPNIIKILR
sp|Q2KIH7|PH4H_BOVIN    RVLRLFEENDINLTHIESRPSRLRKDEYEFFTNLDQRSVPALANIIKILR
sp|P16331|PH4H_MOUSE    KVLRLFEENEINLTHIESRPSRLNKDEYEFFTYLDKRSKPVLGSIIKSLR
tr|G1P4I7|G1P4I7_MYOLU  KVLRLFEENEINLTHIESRPSRLKKDEYEIFTSLDKGSMPTLANIIKILK
sp|P04176|PH4H_RAT      KVLRLFEENDINLTHIESRPSRLNKDEYEFFTYLDKRTKPVLGSIIKSLR
tr|H0VKI3|H0VKI3_CAVPO  RVLRLFEENDINLTHIESRPSRVNKDEYEFFTHLDKRSVPALPSIIQSLR
tr|G3H671|G3H671_CRIGR  -------ENDINLTHIESRPSRVNKDEYEFFTHLDKRSKPALGSIIKSLR
tr|G5AMD7|G5AMD7_HETGA  KVLRLFEENDINLTHIESRPSRLNKDEYEFFTHLDERSVPALSSIIQILR
tr|F7G2Z2|F7G2Z2_MONDO  KVLRLFEENDINLTHIESRPSLLNKDEYEFFTNLDNRSFPALDDLIKSLR
tr|F6QV07|F6QV07_ORNAN  KVLCLFEEKDINLTHIESRPSLLNKDEYEFFINLDNRNIPALDEIIKRLR
tr|G3WCK3|G3WCK3_SARHA  KVLRLFEENDVNLTHIESRPSLLNKDEYEFFTNLDNRSVPALDDLIKSLR
tr|F1SRJ9|F1SRJ9_PIG    --------------------------------------------------
                                                                          

sp|P00439|PH4H_HUMAN    HDIGATVHELSRDKKKDT--------------------------------
tr|G3QF53|G3QF53_GORGO  HDIGATVHELSRDKKKDT--------------------------------
tr|G1R3M2|G1R3M2_NOMLE  NDIGATVHELSRDKKKDT--------------------------------
tr|G7PJC2|G7PJC2_MACFA  HDIGATVHELSRDKKKDT--------------------------------
tr|F7HMW9|F7HMW9_MACMU  HDIGATVHELSRDKKKDT--------------------------------
tr|F7I717|F7I717_CALJA  HDIGATVHELSRDKKKDT--------------------------------
tr|F7BKF9|F7BKF9_HORSE  HDIGATVHELSRDKKKDT--------------------------------
tr|G1T8B6|G1T8B6_RABIT  HDIGATVHELSRDKKKDT--------------------------------
tr|E2R366|E2R366_CANFA  HDIGATVHELSRDKKKDT--------------------------------
tr|H0WTI6|H0WTI6_OTOGA  HDIGATVHELSRDKKKDT--------------------------------
tr|G3TIW0|G3TIW0_LOXAF  HDIGATVHELSREKKKDT--------------------------------
sp|Q2KIH7|PH4H_BOVIN    HDIGATVHELSRDKKKDT--------------------------------
sp|P16331|PH4H_MOUSE    NDIGATVHELSRDKEKNT--------------------------------
tr|G1P4I7|G1P4I7_MYOLU  HEIGATVHELSREKKKDT--------------------------------
sp|P04176|PH4H_RAT      NDIGATVHELSRDKEKNT--------------------------------
tr|H0VKI3|H0VKI3_CAVPO  QDIGATVHELSREKKEDT--------------------------------
tr|G3H671|G3H671_CRIGR  YDIGATVHELSRDKEKNTGLC-----------------------------
tr|G5AMD7|G5AMD7_HETGA  HDIGATVHELSRDKKKDTGLSRCGILNLLTFWPRGAMQYGGSHQLRTLEE
tr|F7G2Z2|F7G2Z2_MONDO  HDIGATVHELSRDKKKDT--------------------------------
tr|F6QV07|F6QV07_ORNAN  HEIGATVHELSRDKKKDT--------------------------------
tr|G3WCK3|G3WCK3_SARHA  HDIGATVHELSRDKKKDT--------------------------------
tr|F1SRJ9|F1SRJ9_PIG    --------------------------------------------------
                                                                          

sp|P00439|PH4H_HUMAN    --------------------------------------------------
tr|G3QF53|G3QF53_GORGO  --------------------------------------------------
tr|G1R3M2|G1R3M2_NOMLE  --------------------------------------------------
tr|G7PJC2|G7PJC2_MACFA  --------------------------------------------------
tr|F7HMW9|F7HMW9_MACMU  --------------------------------------------------
tr|F7I717|F7I717_CALJA  --------------------------------------------------
tr|F7BKF9|F7BKF9_HORSE  --------------------------------------------------
tr|G1T8B6|G1T8B6_RABIT  --------------------------------------------------
tr|E2R366|E2R366_CANFA  --------------------------------------------------
tr|H0WTI6|H0WTI6_OTOGA  --------------------------------------------------
tr|G3TIW0|G3TIW0_LOXAF  --------------------------------------------------
sp|Q2KIH7|PH4H_BOVIN    --------------------------------------------------
sp|P16331|PH4H_MOUSE    --------------------------------------------------
tr|G1P4I7|G1P4I7_MYOLU  --------------------------------------------------
sp|P04176|PH4H_RAT      --------------------------------------------------
tr|H0VKI3|H0VKI3_CAVPO  --------------------------------------------------
tr|G3H671|G3H671_CRIGR  --------------------------------------------------
tr|G5AMD7|G5AMD7_HETGA  ADGAPSACVTSVSPVEGEQDVTALWTEKPVPGPIRCRVVRLCAPFEAVMR
tr|F7G2Z2|F7G2Z2_MONDO  --------------------------------------------------
tr|F6QV07|F6QV07_ORNAN  --------------------------------------------------
tr|G3WCK3|G3WCK3_SARHA  --------------------------------------------------
tr|F1SRJ9|F1SRJ9_PIG    --------------------------------------------------
                                                                          

sp|P00439|PH4H_HUMAN    --------------------------------------------------
tr|G3QF53|G3QF53_GORGO  --------------------------------------------------
tr|G1R3M2|G1R3M2_NOMLE  --------------------------------------------------
tr|G7PJC2|G7PJC2_MACFA  --------------------------------------------------
tr|F7HMW9|F7HMW9_MACMU  --------------------------------------------------
tr|F7I717|F7I717_CALJA  --------------------------------------------------
tr|F7BKF9|F7BKF9_HORSE  --------------------------------------------------
tr|G1T8B6|G1T8B6_RABIT  --------------------------------------------------
tr|E2R366|E2R366_CANFA  --------------------------------------------------
tr|H0WTI6|H0WTI6_OTOGA  --------------------------------------------------
tr|G3TIW0|G3TIW0_LOXAF  --------------------------------------------------
sp|Q2KIH7|PH4H_BOVIN    --------------------------------------------------
sp|P16331|PH4H_MOUSE    --------------------------------------------------
tr|G1P4I7|G1P4I7_MYOLU  --------------------------------------------------
sp|P04176|PH4H_RAT      --------------------------------------------------
tr|H0VKI3|H0VKI3_CAVPO  --------------------------------------------------
tr|G3H671|G3H671_CRIGR  --------------------------------------------------
tr|G5AMD7|G5AMD7_HETGA  GVLAKGTGGDPSALGGLRDKASPASEEPLGARAPGPGPTPRRASAGPSVH
tr|F7G2Z2|F7G2Z2_MONDO  --------------------------------------------------
tr|F6QV07|F6QV07_ORNAN  --------------------------------------------------
tr|G3WCK3|G3WCK3_SARHA  --------------------------------------------------
tr|F1SRJ9|F1SRJ9_PIG    --------------------------------------------------
                                                                          

sp|P00439|PH4H_HUMAN    ------------------------------VPWFPRTIQELDRFANQILS
tr|G3QF53|G3QF53_GORGO  ------------------------------VPWFPRTIQELDRFANQILS
tr|G1R3M2|G1R3M2_NOMLE  ------------------------------VPWFPRTIQELDRFANQILS
tr|G7PJC2|G7PJC2_MACFA  ------------------------------VPWFPRTIQELDRFANQILS
tr|F7HMW9|F7HMW9_MACMU  ------------------------------VPWFPRTIQELDRFANQILS
tr|F7I717|F7I717_CALJA  ------------------------------VPWFPRTIQELDRFANQILS
tr|F7BKF9|F7BKF9_HORSE  ------------------------------VPWFPRTIQELDRFANQILS
tr|G1T8B6|G1T8B6_RABIT  ------------------------------VPWFPRTIQELDRFANQILS
tr|E2R366|E2R366_CANFA  ------------------------------VPWFPRTIQELDRFANQILS
tr|H0WTI6|H0WTI6_OTOGA  ------------------------------VPWFPKTIQELDRFANQILS
tr|G3TIW0|G3TIW0_LOXAF  ------------------------------VPWFPRTIQELDRFANQILS
sp|Q2KIH7|PH4H_BOVIN    ------------------------------VPWFPRTIQELDNFANQVLS
sp|P16331|PH4H_MOUSE    ------------------------------VPWFPRTIQELDRFANQILS
tr|G1P4I7|G1P4I7_MYOLU  ------------------------------VPWFPRTIQDLDRFANQILS
sp|P04176|PH4H_RAT      ------------------------------VPWFPRTIQELDRFANQILS
tr|H0VKI3|H0VKI3_CAVPO  ------------------------------VPWFPRTIQELDRFANQILS
tr|G3H671|G3H671_CRIGR  -------------------------PFCVVVPWFPRTIQELDRFANQILS
tr|G5AMD7|G5AMD7_HETGA  PPGSSSTPKYRGCPKGLGFEGCCLPPFQNDVPWFPRTIQELDRFANQILS
tr|F7G2Z2|F7G2Z2_MONDO  ------------------------------VPWFPRTIQELDRFANQILS
tr|F6QV07|F6QV07_ORNAN  ------------------------------VPWFPRTIQELDKFANQILS
tr|G3WCK3|G3WCK3_SARHA  ------------------------------VPWFPRTIQELDRFANQILS
tr|F1SRJ9|F1SRJ9_PIG    ------------------------------------------------MR
                                                                        : 

sp|P00439|PH4H_HUMAN    YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTW
tr|G3QF53|G3QF53_GORGO  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTW
tr|G1R3M2|G1R3M2_NOMLE  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTW
tr|G7PJC2|G7PJC2_MACFA  YGAELDADHPGFKDPVYRARRKQFADTAYNYRHGQPIPRVEYTEEEKKTW
tr|F7HMW9|F7HMW9_MACMU  YGAELDADHPGFKDPVYRARRKQFADTAYNYRHGQPIPRVEYTEEEKKTW
tr|F7I717|F7I717_CALJA  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPQVEYTEEEKKTW
tr|F7BKF9|F7BKF9_HORSE  YGAELDADHPGFKDPVYRARRKYFADIAYNYQHGQPIPRVEYTEEEKKTW
tr|G1T8B6|G1T8B6_RABIT  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKKTW
tr|E2R366|E2R366_CANFA  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPQVEYTEEEKKTW
tr|H0WTI6|H0WTI6_OTOGA  YGAELDADHPGFKDPVYRARRKQFADIAYSYRHGQPIPRVEYTEEEKKTW
tr|G3TIW0|G3TIW0_LOXAF  YGAELDADHPGFTDPVYRERRKQFADIAYSYRHGQPIPRVEYTEEEKKTW
sp|Q2KIH7|PH4H_BOVIN    YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKKTW
sp|P16331|PH4H_MOUSE    YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEERKTW
tr|G1P4I7|G1P4I7_MYOLU  YGAELDADHPGFKDPVYRARRKYFADIAYNYRHGQPIPRVEYTEEERKTW 
sp|P04176|PH4H_RAT      YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKQTW
tr|H0VKI3|H0VKI3_CAVPO  YGAELDADHPGFKDPVYRARRKHFADIAYNYRHGQPIPRVEYTEEEKKTW
tr|G3H671|G3H671_CRIGR  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKKTW
tr|G5AMD7|G5AMD7_HETGA  YGAELDADHPGFKDPVYRARRKYFADIAYNYCHGQPIPQVEYTEEEKETW
tr|F7G2Z2|F7G2Z2_MONDO  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYTEEEKKTW
tr|F6QV07|F6QV07_ORNAN  YGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRIEYTEEEKKTW
tr|G3WCK3|G3WCK3_SARHA  YGAELDADHPGFKDPVYRTRRKQFADIAYNYRHGQPIPRVEYSEEEKKTW
tr|F1SRJ9|F1SRJ9_PIG    HGAEMDAEDQGFKDPVYRTRRKYFADIAYNYRHGQPIPRVEYTEEEKKTW
                        :***:**:. **.***** *** *** **.* ******::** ***::**

sp|P00439|PH4H_HUMAN    GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTC
tr|G3QF53|G3QF53_GORGO  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTC
tr|G1R3M2|G1R3M2_NOMLE  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTC
tr|G7PJC2|G7PJC2_MACFA  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|F7HMW9|F7HMW9_MACMU  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|F7I717|F7I717_CALJA  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTC
tr|F7BKF9|F7BKF9_HORSE  GTVFRTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEEVSQFLQTC
tr|G1T8B6|G1T8B6_RABIT  GTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|E2R366|E2R366_CANFA  GTVFRTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEEVSQFLQTC
tr|H0WTI6|H0WTI6_OTOGA  GTVFRTLKSLYKTHACYEHNHIFPLLEKYCGFHEDNIPQLEDVSQFLQSC
tr|G3TIW0|G3TIW0_LOXAF  GTVFRTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEEVSQFLQTC
sp|Q2KIH7|PH4H_BOVIN    GTVFRTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEEVSQFLQSC
sp|P16331|PH4H_MOUSE    GTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|G1P4I7|G1P4I7_MYOLU  GTVFKTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQSC
sp|P04176|PH4H_RAT      GTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|H0VKI3|H0VKI3_CAVPO  GTVFRTLKSLYKTHACHEHNHIFPLLEKYCGFREDNIPQLEEVSQFLQTC
tr|G3H671|G3H671_CRIGR  GTVFKTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTC
tr|G5AMD7|G5AMD7_HETGA  GMVFRTLKSLYKTHACHEHNHIFPLLEKYCSFREDNIPQLEDVSQFLQTC
tr|F7G2Z2|F7G2Z2_MONDO  GMVFKTLKSLYKTHACYEHNHIFPLLEKYCGFQEDNIPQLEDVSQFLQSC
tr|F6QV07|F6QV07_ORNAN  GTVFRTLKSLYTTHACYEHNHIFPLLEKYCGFQEGNIPQLEDVSQFLQSC
tr|G3WCK3|G3WCK3_SARHA  GMVFRTLKSLYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQSC
tr|F1SRJ9|F1SRJ9_PIG    GTVFKNLKSLYQTHACYEYNHIFPLLEKYCGFCEDNIPQLEEVSQFLQSC
                        * **:.**:** ****:*:***********.* *.******:******:* 

sp|P00439|PH4H_HUMAN    TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G3QF53|G3QF53_GORGO  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G1R3M2|G1R3M2_NOMLE  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G7PJC2|G7PJC2_MACFA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|F7HMW9|F7HMW9_MACMU  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|F7I717|F7I717_CALJA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|F7BKF9|F7BKF9_HORSE  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G1T8B6|G1T8B6_RABIT  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMFTPEPDICHEL
tr|E2R366|E2R366_CANFA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|H0WTI6|H0WTI6_OTOGA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G3TIW0|G3TIW0_LOXAF  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
sp|Q2KIH7|PH4H_BOVIN    TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
sp|P16331|PH4H_MOUSE    TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G1P4I7|G1P4I7_MYOLU  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
sp|P04176|PH4H_RAT      TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|H0VKI3|H0VKI3_CAVPO  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G3H671|G3H671_CRIGR  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|G5AMD7|G5AMD7_HETGA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHRSKPMYTPEPDICHEL
tr|F7G2Z2|F7G2Z2_MONDO  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|F6QV07|F6QV07_ORNAN  TGFRIRPVAGLLSSRDFLGGLAFRVFHCTQYIRHASKPMYTPEPDICHEL
tr|G3WCK3|G3WCK3_SARHA  TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHEL
tr|F1SRJ9|F1SRJ9_PIG    TGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHASKPMYTPEPDICHEL
                        ****:***************************** ****:**********

sp|P00439|PH4H_HUMAN    LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G3QF53|G3QF53_GORGO  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G1R3M2|G1R3M2_NOMLE  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G7PJC2|G7PJC2_MACFA  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F7HMW9|F7HMW9_MACMU  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F7I717|F7I717_CALJA  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F7BKF9|F7BKF9_HORSE  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G1T8B6|G1T8B6_RABIT  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|E2R366|E2R366_CANFA  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|H0WTI6|H0WTI6_OTOGA  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G3TIW0|G3TIW0_LOXAF  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQE
sp|Q2KIH7|PH4H_BOVIN    LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
sp|P16331|PH4H_MOUSE    LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEG
tr|G1P4I7|G1P4I7_MYOLU  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
sp|P04176|PH4H_RAT      LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEG
tr|H0VKI3|H0VKI3_CAVPO  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G3H671|G3H671_CRIGR  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEG
tr|G5AMD7|G5AMD7_HETGA  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F7G2Z2|F7G2Z2_MONDO  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F6QV07|F6QV07_ORNAN  LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|G3WCK3|G3WCK3_SARHA  LGHVPLFSDPSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQG
tr|F1SRJ9|F1SRJ9_PIG    LGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQE
                        ********* **************************************: 

sp|P00439|PH4H_HUMAN    DSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYY
tr|G3QF53|G3QF53_GORGO  DSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYY
tr|G1R3M2|G1R3M2_NOMLE  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTAIQNYSVTEFQPLYY
tr|G7PJC2|G7PJC2_MACFA  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTAIQKYTVTEFQPLYY
tr|F7HMW9|F7HMW9_MACMU  DSIKAYGAGLLSSFGELQHCLSDKPKLLPLELEKTAIQKYTVTEFQPLYY
tr|F7I717|F7I717_CALJA  DAIKAYGAGLLSSFGELQYCLSDKPTVLPLELEKTAIQKYTVTEFQPLYY
tr|F7BKF9|F7BKF9_HORSE  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLDLEKTAVQEYTITEFQPLYY
tr|G1T8B6|G1T8B6_RABIT  DSIKAYGAGLLSSFGELQYCLSGKPKLLPLELEKTAIQEYPVTEFQPLYY
tr|E2R366|E2R366_CANFA  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTAIQEYTVTEFQPLYY
tr|H0WTI6|H0WTI6_OTOGA  DSIKAYGAGLLSSFGELQYCLSDEPKLLPLDLEKTAVQNYSVTEFQPLYY
tr|G3TIW0|G3TIW0_LOXAF  GSIKAYGAGLLSSFGELQYCLSGKPKLLPLEMEKTAIQEYTITEFQPLYY
sp|Q2KIH7|PH4H_BOVIN    DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTAVQEYTITEFQPLYY
sp|P16331|PH4H_MOUSE    DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTACQEYTVTEFQPLYY
tr|G1P4I7|G1P4I7_MYOLU  DSIKAYGAGLLSSFGELQYCLSDKPMLLPLDLEKTAIQEYTITEFQPLYY
sp|P04176|PH4H_RAT      DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTACQEYSVTEFQPLYY
tr|H0VKI3|H0VKI3_CAVPO  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLEMEKTAVQPYPVTEFQPVYY
tr|G3H671|G3H671_CRIGR  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLDLEKTASQEYNVTEFQPLYY
tr|G5AMD7|G5AMD7_HETGA  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKMAVQPYTITEFQPLYY
tr|F7G2Z2|F7G2Z2_MONDO  NSIKAYGAGLLSSFGELQYCLSDKPKLLPLELETTAVQEYPVTEFQPVYF
tr|F6QV07|F6QV07_ORNAN  GSIKAYGAGLLSSFGELQYCLSEKPQLLPLDLEKTSVQKYGITEFQPIYF
tr|G3WCK3|G3WCK3_SARHA  DSIKAYGAGLLSSFGELQYCLSDKPKLLPLELEKTAVQEYPVTEFQPVYF
tr|F1SRJ9|F1SRJ9_PIG    DSIKAYGAGLLSSFGELQYCLSGEPKLLTLELEKTAVQEYTVTEFQPVYY
                        .:****************:*** :* :*.*::*. : * * :*****:*: 

sp|P00439|PH4H_HUMAN    VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|G3QF53|G3QF53_GORGO  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|G1R3M2|G1R3M2_NOMLE  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|G7PJC2|G7PJC2_MACFA  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|F7HMW9|F7HMW9_MACMU  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|F7I717|F7I717_CALJA  VAESFNDAKEKVRNFAATIPRPFSVHYDPYTQRIEVLDNTQQLKILADSI
tr|F7BKF9|F7BKF9_HORSE  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|G1T8B6|G1T8B6_RABIT  VAESFMDAKEKVRNFAATIPRPFSVRYDPYTQRIEILDNTQQLKILADSI
tr|E2R366|E2R366_CANFA  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
tr|H0WTI6|H0WTI6_OTOGA  VAESFSDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNIQQLKILADSI
tr|G3TIW0|G3TIW0_LOXAF  VAESFNDAKEKVRDFAATIPRPFSVHYDPYTQRIEVLDNTEQLKILADSI
sp|Q2KIH7|PH4H_BOVIN    VAESFNDAKEKVRNFAATIPRPFSVHYDPYTQRIEVLDNTQQLKILADSI
sp|P16331|PH4H_MOUSE    VAESFNDAKEKVRTFAATIPRPFSVRYDPYTQRVEVLDNTQQLKILADSI
tr|G1P4I7|G1P4I7_MYOLU  VAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
sp|P04176|PH4H_RAT      VAESFSDAKEKVRTFAATIPRPFSVRYDPYTQRVEVLDNTQQLKILADSI
tr|H0VKI3|H0VKI3_CAVPO  VAESFSDAQEKVRNFAATIPRPFSVHYNPYTQRIEVLDNAQQLKILADSI
tr|G3H671|G3H671_CRIGR  VAESFNDAKEKVRAFAATIPRPFSVRYDPYTQRVEVLDNTQQLKILADSI
tr|G5AMD7|G5AMD7_HETGA  VAESFSDAKEKVRNFAATIPRPFSVHYDPYTQRIEVLDNTQQLKILADSI
tr|F7G2Z2|F7G2Z2_MONDO  VAESFSDAKEKVRAFASTIPRPFSVRYDPYTQRIEVLDNTKQLKILADSI
tr|F6QV07|F6QV07_ORNAN  VAESFKDAKEKVRNFASTIPRPFSVRYDPYTQRIEVLDNTDQLKILADSI
tr|G3WCK3|G3WCK3_SARHA  VAESFNDAKDKVRNFASTIPRPFSVRYDPYTQRIEVLDNTKQLKILADSI
tr|F1SRJ9|F1SRJ9_PIG    VAESFSDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSI
                        ***** **::*** **:********:*:*****:*:*** .*********

sp|P00439|PH4H_HUMAN    NSEIGILCSALQKI--K
tr|G3QF53|G3QF53_GORGO  NSEIGILCSALQKIKKS
tr|G1R3M2|G1R3M2_NOMLE  NSEIGILCSALQKI--K
tr|G7PJC2|G7PJC2_MACFA  NSEIGILCSALQKI--K
tr|F7HMW9|F7HMW9_MACMU  NSEIGILCSALQKI--K
tr|F7I717|F7I717_CALJA  NSEIGILCSALQKI--K
tr|F7BKF9|F7BKF9_HORSE  NSEVGILCSALQKI--K
tr|G1T8B6|G1T8B6_RABIT  NSEVGILCNALQKNK-S
tr|E2R366|E2R366_CANFA  NSEVGILCSALQKI--K
tr|H0WTI6|H0WTI6_OTOGA  DSEVGILCSALQKI--K
tr|G3TIW0|G3TIW0_LOXAF  NSEVGILCSALQKI--K
sp|Q2KIH7|PH4H_BOVIN    SSEVEILCSALQKL--K
sp|P16331|PH4H_MOUSE    NSEVGILCHALQKIK-S
tr|G1P4I7|G1P4I7_MYOLU  NSEVGILCSALQKIKKS
sp|P04176|PH4H_RAT      NSEVGILCNALQKIK-S
tr|H0VKI3|H0VKI3_CAVPO  NSEVGILCSALQKI--K
tr|G3H671|G3H671_CRIGR  NRTNE----TSPYT--P
tr|G5AMD7|G5AMD7_HETGA  NRSNGY-HSACSLI--T
tr|F7G2Z2|F7G2Z2_MONDO  SCEFGILCSALQKI--K
tr|F6QV07|F6QV07_ORNAN  SGEVGILCGALQKI--K
tr|G3WCK3|G3WCK3_SARHA  NCEFGILCSALQKI--K
tr|F1SRJ9|F1SRJ9_PIG    NGEVGILCNALQKL--K
                        .        :