CD task7 protocol

Choosing structure

So, we decided to use 2O4H, since it has a bound substrate, low resolution and a listed pH value.

Apo-structure:       2O53 Resolution: 2,7,  R-free: 0,269, pH: 6.0, chains: A,B
Holo-structure:      2O4H Resolution: 2,7,  R-free: 0,271, pH: 6.0, chains: A,B intermediate substrate analog: N-phosphonomethyl-L-aspartate
Apo-structure:       2I3C Resolution: 2,8,  R-free: 0,243, pH: -  , chains: A,B
Ensemble Refinement  2Q51 Resolution: 2,8,  R-free: 0,239, pH: -  , chains: A,B

For 2O4H, there are only missing residues at the N- and C-terminal ends, which can be neglected:

REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) 
REMARK 465   M RES C SSSEQI                                                     
REMARK 465   1 ALA A    -1                                                      
REMARK 465   1 ILE A     0                                                      
REMARK 465   1 ALA A     1                                                      
REMARK 465   1 THR A     2                                                      
REMARK 465   1 SER A     3                                                      
REMARK 465   1 CYS A     4                                                      
REMARK 465   1 HIS A     5                                                      
REMARK 465   1 ILE A     6                                                      
REMARK 465   1 ALA A     7                                                      
REMARK 465   1 GLU A     8                                                      
REMARK 465   1 CYS A   311                                                      
REMARK 465   1 LEU A   312                                                      
REMARK 465   1 HIS A   313