Difference between revisions of "CD task7 protocol"

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(Created page with "== Choosing structure == Apo-structure: 2O53 Resolution: 2,7, R-free: 0,269, pH: 6.0, chains: A,B Holo-structure: 2O4H Resolution: 2,7, R-free: 0,271, pH: 6.0, ch…")
 
(Choosing structure)
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== Choosing structure ==
 
== Choosing structure ==
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So, we decided to use 2O4H, since it has a bound substrate, low resolution and a listed pH value.
   
 
Apo-structure: 2O53 Resolution: 2,7, R-free: 0,269, pH: 6.0, chains: A,B
 
Apo-structure: 2O53 Resolution: 2,7, R-free: 0,269, pH: 6.0, chains: A,B

Revision as of 13:26, 20 June 2012

Choosing structure

So, we decided to use 2O4H, since it has a bound substrate, low resolution and a listed pH value.

Apo-structure:       2O53 Resolution: 2,7,  R-free: 0,269, pH: 6.0, chains: A,B
Holo-structure:      2O4H Resolution: 2,7,  R-free: 0,271, pH: 6.0, chains: A,B intermediate substrate analog: N-phosphonomethyl-L-aspartate
Apo-structure:       2I3C Resolution: 2,8,  R-free: 0,243, pH: -  , chains: A,B
Ensemble Refinement  2Q51 Resolution: 2,8,  R-free: 0,239, pH: -  , chains: A,B