Difference between revisions of "CD task6 protocol"
From Bioinformatikpedia
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(→Multiple Sequence Alignment) |
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==Multiple Sequence Alignment== |
==Multiple Sequence Alignment== |
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| − | We searched UniProt_mammals with a simple Blast Search and extracted non |
+ | We searched UniProt_mammals with a simple Blast Search and extracted non redundant sequences by hand. Thus we found 29 homologues. Then we used T-Coffee Expresso for the generation of the MSA. |
sp|P45381|ACY2_HUMAN MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64 |
sp|P45381|ACY2_HUMAN MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64 |
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Revision as of 22:03, 19 June 2012
Contents
Blosum62
# Matrix made by matblas from blosum62.iij # * column uses minimum score # BLOSUM Clustered Scoring Matrix in 1/2 Bit Units # Blocks Database = /data/blocks_5.0/blocks.dat # Cluster Percentage: >= 62 # Entropy = 0.6979, Expected = -0.5209 A R N D C Q E G H I L K M F P S T W Y V B Z X * A 4 -1 -2 -2 0 -1 -1 0 -2 -1 -1 -1 -1 -2 -1 1 0 -3 -2 0 -2 -1 0 -4 R -1 5 0 -2 -3 1 0 -2 0 -3 -2 2 -1 -3 -2 -1 -1 -3 -2 -3 -1 0 -1 -4 N -2 0 6 1 -3 0 0 0 1 -3 -3 0 -2 -3 -2 1 0 -4 -2 -3 3 0 -1 -4 D -2 -2 1 6 -3 0 2 -1 -1 -3 -4 -1 -3 -3 -1 0 -1 -4 -3 -3 4 1 -1 -4 C 0 -3 -3 -3 9 -3 -4 -3 -3 -1 -1 -3 -1 -2 -3 -1 -1 -2 -2 -1 -3 -3 -2 -4 Q -1 1 0 0 -3 5 2 -2 0 -3 -2 1 0 -3 -1 0 -1 -2 -1 -2 0 3 -1 -4 E -1 0 0 2 -4 2 5 -2 0 -3 -3 1 -2 -3 -1 0 -1 -3 -2 -2 1 4 -1 -4 G 0 -2 0 -1 -3 -2 -2 6 -2 -4 -4 -2 -3 -3 -2 0 -2 -2 -3 -3 -1 -2 -1 -4 H -2 0 1 -1 -3 0 0 -2 8 -3 -3 -1 -2 -1 -2 -1 -2 -2 2 -3 0 0 -1 -4 I -1 -3 -3 -3 -1 -3 -3 -4 -3 4 2 -3 1 0 -3 -2 -1 -3 -1 3 -3 -3 -1 -4 L -1 -2 -3 -4 -1 -2 -3 -4 -3 2 4 -2 2 0 -3 -2 -1 -2 -1 1 -4 -3 -1 -4 K -1 2 0 -1 -3 1 1 -2 -1 -3 -2 5 -1 -3 -1 0 -1 -3 -2 -2 0 1 -1 -4 M -1 -1 -2 -3 -1 0 -2 -3 -2 1 2 -1 5 0 -2 -1 -1 -1 -1 1 -3 -1 -1 -4 F -2 -3 -3 -3 -2 -3 -3 -3 -1 0 0 -3 0 6 -4 -2 -2 1 3 -1 -3 -3 -1 -4 P -1 -2 -2 -1 -3 -1 -1 -2 -2 -3 -3 -1 -2 -4 7 -1 -1 -4 -3 -2 -2 -1 -2 -4 S 1 -1 1 0 -1 0 0 0 -1 -2 -2 0 -1 -2 -1 4 1 -3 -2 -2 0 0 0 -4 T 0 -1 0 -1 -1 -1 -1 -2 -2 -1 -1 -1 -1 -2 -1 1 5 -2 -2 0 -1 -1 0 -4 W -3 -3 -4 -4 -2 -2 -3 -2 -2 -3 -2 -3 -1 1 -4 -3 -2 11 2 -3 -4 -3 -2 -4 Y -2 -2 -2 -3 -2 -1 -2 -3 2 -1 -1 -2 -1 3 -3 -2 -2 2 7 -1 -3 -2 -1 -4 V 0 -3 -3 -3 -1 -2 -2 -3 -3 3 1 -2 1 -1 -2 -2 0 -3 -1 4 -3 -2 -1 -4 B -2 -1 3 4 -3 0 1 -1 0 -3 -4 0 -3 -3 -2 0 -1 -4 -3 -3 4 1 -1 -4 Z -1 0 0 1 -3 3 4 -2 0 -3 -3 1 -1 -3 -1 0 -1 -3 -2 -2 1 4 -1 -4 X 0 -1 -1 -1 -2 -1 -1 -1 -1 -1 -1 -1 -1 -1 -2 0 0 -2 -1 -1 -1 -1 -1 -4 * -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 -4 1
PAM1
1 PAM evolutionary distance
Ala Arg Asn Asp Cys Gln Glu Gly His Ile Leu Lys Met Phe Pro Ser Thr Trp Tyr Val
A R N D C Q E G H I L K M F P S T W Y V
Ala A 9867 2 9 10 3 8 17 21 2 6 4 2 6 2 22 35 32 0 2 18
Arg R 1 9913 1 0 1 10 0 0 10 3 1 19 4 1 4 6 1 8 0 1
Asn N 4 1 9822 36 0 4 6 6 21 3 1 13 0 1 2 20 9 1 4 1
Asp D 6 0 42 9859 0 6 53 6 4 1 0 3 0 0 1 5 3 0 0 1
Cys C 1 1 0 0 9973 0 0 0 1 1 0 0 0 0 1 5 1 0 3 2
Gln Q 3 9 4 5 0 9876 27 1 23 1 3 6 4 0 6 2 2 0 0 1
Glu E 10 0 7 56 0 35 9865 4 2 3 1 4 1 0 3 4 2 0 1 2
Gly G 21 1 12 11 1 3 7 9935 1 0 1 2 1 1 3 21 3 0 0 5
His H 1 8 18 3 1 20 1 0 9912 0 1 1 0 2 3 1 1 1 4 1
Ile I 2 2 3 1 2 1 2 0 0 9872 9 2 12 7 0 1 7 0 1 33
Leu L 3 1 3 0 0 6 1 1 4 22 9947 2 45 13 3 1 3 4 2 15
Lys K 2 37 25 6 0 12 7 2 2 4 1 9926 20 0 3 8 11 0 1 1
Met M 1 1 0 0 0 2 0 0 0 5 8 4 9874 1 0 1 2 0 0 4
Phe F 1 1 1 0 0 0 0 1 2 8 6 0 4 9946 0 2 1 3 28 0
Pro P 13 5 2 1 1 8 3 2 5 1 2 2 1 1 9926 12 4 0 0 2
Ser S 28 11 34 7 11 4 6 16 2 2 1 7 4 3 17 9840 38 5 2 2
Thr T 22 2 13 4 1 3 2 2 1 11 2 8 6 1 5 32 9871 0 2 9
Trp W 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 9976 1 0
Tyr Y 1 0 3 0 3 0 1 0 4 1 1 0 0 21 0 1 1 2 9945 1
Val V 13 2 1 1 3 2 2 3 3 57 11 1 17 1 3 2 10 0 2 9901
PAM250
250 PAM evolutionary distance
ORIGINAL AMINO ACID
Ala Arg Asn Asp Cys Gln Glu Gly His Ile Leu Lys Met Phe Pro Ser Thr Trp Tyr Val
A R N D C Q E G H I L K M F P S T W Y V
Ala A 13 6 9 9 5 8 9 12 6 8 6 7 7 4 11 11 11 2 4 9
Arg R 3 17 4 3 2 5 3 2 6 3 2 9 4 1 4 4 3 7 2 2
Asn N 4 4 6 7 2 5 6 4 6 3 2 5 3 2 4 5 4 2 3 3
Asp D 5 4 8 11 1 7 10 5 6 3 2 5 3 1 4 5 5 1 2 3
Cys C 2 1 1 1 52 1 1 2 2 2 1 1 1 1 2 3 2 1 4 2
Gln Q 3 5 5 6 1 10 7 3 7 2 3 5 3 1 4 3 3 1 2 3
Glu E 5 4 7 11 1 9 12 5 6 3 2 5 3 1 4 5 5 1 2 3
Gly G 12 5 10 10 4 7 9 27 5 5 4 6 5 3 8 11 9 2 3 7
His H 2 5 5 4 2 7 4 2 15 2 2 3 2 2 3 3 2 2 3 2
Ile I 3 2 2 2 2 2 2 2 2 10 6 2 6 5 2 3 4 1 3 9
Leu L 6 4 4 3 2 6 4 3 5 15 34 4 20 13 5 4 6 6 7 13
Lys K 6 18 10 8 2 10 8 5 8 5 4 24 9 2 6 8 8 4 3 5
Met M 1 1 1 1 0 1 1 1 1 2 3 2 6 2 1 1 1 1 1 2
Phe F 2 1 2 1 1 1 1 1 3 5 6 1 4 32 1 2 2 4 20 3
Pro P 7 5 5 4 3 5 4 5 5 3 3 4 3 2 20 6 5 1 2 4
Ser S 9 6 8 7 7 6 7 9 6 5 4 7 5 3 9 10 9 4 4 6
Thr T 8 5 6 6 4 5 5 6 4 6 4 6 5 3 6 8 11 2 3 6
Trp W 0 2 0 0 0 0 0 0 1 0 1 0 0 1 0 1 0 55 1 0
Tyr Y 1 1 2 1 3 1 1 1 3 2 2 1 2 15 1 2 2 3 31 2
Val V 7 4 4 4 4 4 4 4 5 4 15 10 4 10 5 5 5 72 4 17
Create position specific substitution matrix
blastpgp -d /mnt/project/pracstrucfunc12/data/big/big_80 -i p45381_wt.fa -o psiblast_it2_p45381_wt_big80.out -j 5 -Q pssm.out
Multiple Sequence Alignment
We searched UniProt_mammals with a simple Blast Search and extracted non redundant sequences by hand. Thus we found 29 homologues. Then we used T-Coffee Expresso for the generation of the MSA.
sp|P45381|ACY2_HUMAN MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|H2QBW4|H2QBW4_PANTR MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|G3QQC1|G3QQC1_GORGO MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
sp|Q5R9E0|ACY2_PONAB MTSCHIA-----EE----------P-IQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|G1S5Z4|G1S5Z4_NOMLE MTSCHIA-----EE----------H-IQKVAIFGGTHGNELTGVFLVKYWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|G7PT66|G7PT66_MACFA MTSCHIA-----EE----------P-IKKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|F7DR53|F7DR53_MACMU MTSCHIA-----EE----------P-IKKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|F6WMI4|F6WMI4_HORSE ------M-----TS-CH----NAEDPIKKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVTPFITNPRAVKKCTRY 64
sp|B1PK17|ACY2_PIG -MTSCQV-----AE----------DPIKKVAIFGGTHGNELTGVFLVKHWLENNTEIQRTGLEVKPFITNPRAVEKCTRY 64
tr|G3TAV8|G3TAV8_LOXAF MTSCHVA-----EE----------P-IKKVAIFGGTHGNELTGVFLVKHWLEDGAEIQRAGLEVKPFITNPRAVKKCSRY 64
sp|P46446|ACY2_BOVIN ------M-----TS-----CHVAEDPIKKVAIFGGTHGNELTGVFLVKHWLENSTEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|G1SPT6|G1SPT6_RABIT ------------MSSCH---VAEAP-IKKIAIFGGTHGNELTGVFLVKHWLQNGAEIQRTGLEVRPFITNPRAVEKCTRY 64
tr|H0WW85|H0WW85_OTOGA MTSCHIA-----EE----------P-IKKVAIFGGTHGNELTGVFLVKHWLQNGAEIQRTGLEIKPLITNPKAVKKCTRY 64
tr|G1P679|G1P679_MYOLU -------------MTYH----VVEDPIKKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRY 63
tr|E2R8M6|E2R8M6_CANFA -------------M--TSYHVTEDP-IKKVAIFGGTHGNELTGVFLVKHWLENDTEIQRTGLEVKPFITNPRAVKKCTRY 64
tr|G5B939|G5B939_HETGA ------M-----TE----------KPIKKIAIFGGTHGNELTGVFLVKHWLQDGAEIQRTGLEVKPFISNPRAVKKCLRY 59
sp|Q9R1T5|ACY2_RAT -MTSCVA-----EE----------P-IKKIAIFGGTHGNELTGVFLVTHWLKNGAEVHRAGLEVKPFITNPRAVEKCTRY 63
tr|B2KFD4|B2KFD4_MOUSE -MTSCVA-----KE----------P-IKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRY 63
tr|F6ZFN3|F6ZFN3_ORNAN MTSGQVV-----CE--T--------PVRRIAIFGGTHGNELSGVFLVKHWQENGTEIQRTGLEVNPFITNPRAVEKCTRY 65
tr|H9H8M1|H9H8M1_MONDO MAAPRGA-----RT--P---------LTRVAIFGGTHGNELSGVLLARHWQEDGAEVQRPGLRVTPFISNPRAVHRGVRY 64
tr|G3VQ32|G3VQ32_SARHA ----MCS-----TAS------MGHR-LNRVAVMGGTHGNELSGVYLVKHWLQDPSELQRRTFTVTPFLANPKATKRCMRY 64
sp|Q5M876|ACY3_RAT MCSLPGS-----RKP------L-----LRVAVTGGTHGNEMCGVYLARYWLQNPGELQRPSFSAMPVLANPAATAACRRY 64
tr|H0WH68|H0WH68_OTOGA ----MCS-RPVPREP------L-----HRVAVTGGTHGNEMSGICLVRHWLQAPGELQRPSFSAVPVLANLAAIAACRRY 64
tr|H0UYR8|H0UYR8_CAVPO ----MCS-LPVPQEP------L-----RRVAVTGGTHGNEMSGVYLVRQWLRTPVDLQRPSFSAVPVLANPAATAVCRRY 64
tr|G1R3Y2|G1R3Y2_NOMLE ----MCS-LPVPREP------L-----RRVAVTGGTHGNEMSGIYLARHWLRAPAELQRPSFSAVPVLANPAATAGCRRY 64
tr|F7A210|F7A210_MACMU ----MCS-LPVPREP------L-----RRVAVTGGTHGNEMSGVYLARHWLQAPAELQRLSFSAVPVLANPAATAACRRY 64
tr|G7PP64|G7PP64_MACFA ----MCS-LPVPREP------L-----RRVAVTGGTHGNEMSGVYLARHWLQAPAELERLSFSAVPVLANPAATAACRRY 64
tr|G3I3Y2|G3I3Y2_CRIGR -----MSSLPVPREP------L-----RRVAVTGGTHGNEMCGIHLIRHWLQAPGELQRPSFSAMPVLANPAATAACRRY 64
tr|G1PIL7|G1PIL7_MYOLU ----MCS-RPAPREP------L-----RRVAVIGGTHGNEMSGVHLARHWLRALGELQRPSFSAVPVLANPAATAACRRY 64
::*: *******: *: * * . ::.* : *.::* * **
sp|P45381|ACY2_HUMAN IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|H2QBW4|H2QBW4_PANTR IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|G3QQC1|G3QQC1_GORGO IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
sp|Q5R9E0|ACY2_PONAB IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|G1S5Z4|G1S5Z4_NOMLE IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|G7PT66|G7PT66_MACFA IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEIYHLFGPKDGEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|F7DR53|F7DR53_MACMU IDCDLNRIFDLENLGKKMSEDLPYEVRRAQEIYHLFGPKDGEDSYDIIFDLHNTTSNMGCTLILED--SRNN--FLIQMF 140
tr|F6WMI4|F6WMI4_HORSE IDCDLNRVFDLENLGKKMSEDLPYEVRRAQEINHLFGPKNSEDSYDIIFDLHNTTSNMGCTLILED--SRND--FLIQMF 140
sp|B1PK17|ACY2_PIG IDCDLNRVFDPENLGKRMSEDLPYEVRRAQEISRLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRND--FLIQMF 140
tr|G3TAV8|G3TAV8_LOXAF IDCDLNRVFDLENLGKNRSEDLPYEVIRAQEINHLFGPKESEDSYDIIFDLHNTTSNMGCTLILED--SKND--FLIQMF 140
sp|P46446|ACY2_BOVIN IDCDLNRVFDPENLGKKKSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRND--FLIQMF 140
tr|G1SPT6|G1SPT6_RABIT IDCDLNRIFDLENLSKEVSEDLPYEVRRAQEINRLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRND--FLIQMF 140
tr|H0WW85|H0WW85_OTOGA IDCDLNRVFDSENLGKEISEDLPYEVRRAQEINLLFGPKHSEDSYDIIFDLHNTTSNMGCTLILED--SRNN--LLIQMF 140
tr|G1P679|G1P679_MYOLU IDCDLNRVFDHGNLGKKLSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILED--SRND--FLIQMF 139
tr|E2R8M6|E2R8M6_CANFA IDCDLNRVFDPENLGKKVSKDLPYEVRRAQEINHLFGPKDNEDSYDIIFDLHNTTSNMGCTLILED--SRND--LLIQMF 140
tr|G5B939|G5B939_HETGA IDCDLNRVFDLENLGKKMSEDLPYEVRRAQEINHLFGQKNSEDSYDIIFDLHNTTSNMGCTLILED--SKND--FLIQMF 135
sp|Q9R1T5|ACY2_RAT IDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDVVFDLHNTTSNMGCTLILED--SRND--FLIQMF 139
tr|B2KFD4|B2KFD4_MOUSE IDCDLNRVFDLENLSKEMSEDLPYEVRRAQEINHLFGPKNSDDAYDIVFDLHNTTSNMGCTLILED--SRND--FLIQMF 139
tr|F6ZFN3|F6ZFN3_ORNAN IDCDLNRVFDLENLCKKETENLPYEIRRAQEINHLFGPKGSEDAYDLIFDLHNTTSNTGCTIILEN--SKND--FIIQMV 141
tr|H9H8M1|H9H8M1_MONDO VDRDLNRVFDPAHLGPEPPEDQPYEVEQAREIHRLFG------AYDVILDLHNTTSNMGCTLILES--SRDD--VLIQMF 134
tr|G3VQ32|G3VQ32_SARHA ISQDLNRSFTKAFLLSKGTKDDLYEVSRAQEINQLFGPRGSADAFDFIFDLHNTTSNMAACLISDSEKN-V---FTMHMC 140
sp|Q5M876|ACY3_RAT IDRDLNRTFTLTFLGSTATPDDPYEVKRAQELNQLLGPKGTCQAFDFILDLHNTTANTGACLISEV-SQ-NP--FNLHLC 140
tr|H0WH68|H0WH68_OTOGA VGCDLNRTFISTFLTAKASPDDPYEVTRARELNQLLGPKASSQAFDFVLDLHNTTANVGTCLIIDT--N-QD-VFAMHLC 140
tr|H0UYR8|H0UYR8_CAVPO IGRDLNRTFTSTFLTSKATPDELYEVTRARELNHLLGPKNSSQAFDFILDLHNTTANMGTCFIL----N-VTDTFAMHLC 139
tr|G1R3Y2|G1R3Y2_NOMLE VDHDLNRTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGRAFDFVLDLHNTTANVGTCLIAKS--S-HE-VFAMHLC 140
tr|F7A210|F7A210_MACMU VDHDLNRAFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGRAFDFVLDLHNTTANMGTCLIAKS--S-HE-VFAMHLC 140
tr|G7PP64|G7PP64_MACFA VDHDLNRAFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGRAFDFVLDLHNTTANMGTCLIAKS--S-HE-VFAMHLC 140
tr|G3I3Y2|G3I3Y2_CRIGR MDRDLNRTFTFTFLSSTATPNDPYEVTRAQELNQLLGPKGTDQAFDFILDLHNTTANTGVCLISE--AS-HS-PFNLHLC 140
tr|G1PIL7|G1PIL7_MYOLU VDCDLNRAFTGAFLTARAHPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTASMGTCLIESS-HN-V---FAMHLC 139
:. **** * * : **: :*:*: *:* ::*.::******:. . :* . . :::
sp|P45381|ACY2_HUMAN HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|H2QBW4|H2QBW4_PANTR HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|G3QQC1|G3QQC1_GORGO HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
sp|Q5R9E0|ACY2_PONAB HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|G1S5Z4|G1S5Z4_NOMLE HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|G7PT66|G7PT66_MACFA HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|F7DR53|F7DR53_MACMU HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCA 219
tr|F6WMI4|F6WMI4_HORSE HYIKTSLA-PLPCYVYLIEHPSLKYATTRSVAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHNFNEGKEFPPCA 219
sp|B1PK17|ACY2_PIG HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHNFNEGKEFPPCA 219
tr|G3TAV8|G3TAV8_LOXAF HYIKTSLA-PSPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPSCA 219
sp|P46446|ACY2_BOVIN HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIQHALDFIHNFNEGKEFPPCA 219
tr|G1SPT6|G1SPT6_RABIT HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKIIKHALDFVHHFNKGKEFPPCA 219
tr|H0WW85|H0WW85_OTOGA HYIKTSLA-PLPCYVYLIEHPTFKYATTRSVAKYPVGIEVGPQPHGVLRADILNQMRKMIKHALDFIHDFNEGKEFPPCA 219
tr|G1P679|G1P679_MYOLU HYIKTSLA-PLPCYIYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKYALDFIHNFNEGKEFPPCA 218
tr|E2R8M6|E2R8M6_CANFA HYIKNSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKYALDFIHNFNEGKEFPPCA 219
tr|G5B939|G5B939_HETGA HYIKTSLA-PLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHQFNQGKEFPPCA 214
sp|Q9R1T5|ACY2_RAT HYIKTCMA-PLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRRMLKHALDFIQRFNEGKEFPPCA 218
tr|B2KFD4|B2KFD4_MOUSE HYIKTCMA-PLPCSVYLIEHPSLKYATTRSIAKYPVGIEVGPQPHGVLRADILDQMRKMIKHALDFIQHFNEGKEFPPCS 218
tr|F6ZFN3|F6ZFN3_ORNAN HYIQNSLA-PLPCYVYLIEHPTLKYSTTRSIAKYPVGIEVGPQPQGVLRADILDQMRKIIKHALDFVQHFNEGREFPPCT 220
tr|H9H8M1|H9H8M1_MONDO RYIQVPPGRPAPPYVDSSQEPPLQEADLPPWASLPFGRGVGVDFQGLAKVEVKTWARVFFRVSCLF-----PGREFPACS 209
tr|G3VQ32|G3VQ32_SARHA HYIQVKHT-QTPCPIYLYGLPGQENYSLMSVAKNSLGLELGPQPHGVLRADVSAHMRALLACGLDFIDLFNQGTSFPAFD 219
sp|Q5M876|ACY3_RAT HYLQLQNP-GLPCRLFQFEPPGTESYSMDSVSKNGISLELGPQPQGVLRAELFSQMRAMVASILDFIELFNQGMEFPAFE 219
tr|H0WH68|H0WH68_OTOGA HHLQLQNP-ELPCRVILYQRPGEERFSLSSVTKNGISLELGPQPQGVLRADLFSQMRALVAAVLDFIELFNQGTAFPAFE 219
tr|H0UYR8|H0UYR8_CAVPO HYLQLQNS-EPPSRVLVIEASEDTTFSVDSVAKHGMTVELGPQPQGVLRADLFSRMRSLVASVLDFIELFNQGAAFPAFE 218
tr|G1R3Y2|G1R3Y2_NOMLE RHLQLQYP-ELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFE 219
tr|F7A210|F7A210_MACMU RHLQLQYP-ELSCQVFLCQRSGEESYNLDSVAKNGLALELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFE 219
tr|G7PP64|G7PP64_MACFA RHLQLQYP-ELSCQVFLCQRSGEESYNLDSVAKNGLALELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFE 219
tr|G3I3Y2|G3I3Y2_CRIGR HYLQLQNP-GLPCRLFQYDISGRETYSVESVSKDGICLELGPQPQGVLRADLFSQMRTLVGSILDFIELFNQGMAFPAFE 219
tr|G1PIL7|G1PIL7_MYOLU RHLQLQSP-ELPCRVFLYQLPGEETYSVNSVAKSGLGLELGPQPQGVLRADLLVRMRALVAAALDFIQLFNQGTEFPAFE 218
:::: . : . . :. . :* : :*: :.:: * :. * * **.
sp|P45381|ACY2_HUMAN IEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|H2QBW4|H2QBW4_PANTR IEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G3QQC1|G3QQC1_GORGO IEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
sp|Q5R9E0|ACY2_PONAB IEVYKIIEKVDYPRDENGEIAAVIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G1S5Z4|G1S5Z4_NOMLE IEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPVFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G7PT66|G7PT66_MACFA IEVYKIIEKVDYPRDENGEIAAVIHPNLQDQDWKPLHPGDPMFLTLDGKTMPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|F7DR53|F7DR53_MACMU IEVYKIIEKVDYPRDENGEIAAVIHPNLQDQDWKPLHPGDPMFLTLDGKTMPLGGDCIVYPVFVNEAAYYEKKEAFAKTT 299
tr|F6WMI4|F6WMI4_HORSE IEVHKIMEKVDYPRNENGEIAAFIHPNLQDQDWKPLYPGDPVFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
sp|B1PK17|ACY2_PIG IEVYKIMEKVDYPRNESGEIAAIIHPKLQDQDWKPLHPGDPVFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G3TAV8|G3TAV8_LOXAF IEVYKIMEKVDYPRNESGEIAAVIHPNLQDQDWKPLHPGDPMFLTLDGKTIALDGDCTVYPVFVNEAAYYEKKEAFAKTT 299
sp|P46446|ACY2_BOVIN IEVYKIMRKVDYPRNESGEISAIIHPKLQDQDWKPLHPEDPVFLTLDGKTIPLGGDQTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G1SPT6|G1SPT6_RABIT IEVYKIMEKVDYPRNKSGEIAAVIHPNLQDQDWKPLHPGDPVFLTLDGRLIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 299
tr|H0WW85|H0WW85_OTOGA IEVYKIMEKVDYPRNENGEISAVIHPDLQDQDWKPLHPGDPVFLTLDGKTIPLGGDCTVYPVFINEASYYEKKEAFAKTT 299
tr|G1P679|G1P679_MYOLU IEVYKIMEKVDYPRNENGEIAAIIHPNLQDQDWKPLRPEDPVFLTLDGKTIPLGGDCTVYPTFVNEAAYYEKKEAFAKTT 298
tr|E2R8M6|E2R8M6_CANFA IEVYKIMEKVDYPRNENGDIAALIHPNLQDQDWKPLHPGDPVFLTLEGKSIPLGGDSTVYPVFVNEAAYYEKKEAFAKTT 299
tr|G5B939|G5B939_HETGA IEAYKIMEKVDFPRNENGEIAAIIHPNLQDQDWKPLHPGDPVFVTLDGKTILLGGDCTVYPVFVNEVAYYEKKEAFAKTT 294
sp|Q9R1T5|ACY2_RAT IDVYKIMEKVDYPRNESGDVAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 298
tr|B2KFD4|B2KFD4_MOUSE IDVYKIMEKVDYPRNESGDMAAVIHPNLQDQDWKPLHPGDPVFVSLDGKVIPLGGDCTVYPVFVNEAAYYEKKEAFAKTT 298
tr|F6ZFN3|F6ZFN3_ORNAN IEAYKLIEKVDFPRNKKGEITALIHQSLQDQDWKPLNPGDPVFLTLDGKIIPFDGDCVVYPVFVNEAAYYEKKEAFAKSL 300
tr|H9H8M1|H9H8M1_MONDO LEAYQVTGKVDYPRKANGEIAAVIHPSLQDRDWQPLKPGEPAFLTLDGTTIPFDGDGPVYPVFVNEAAYYEKGEAFAKTR 289
tr|G3VQ32|G3VQ32_SARHA IVAYKLIESVHFPRSPDGEFLGIVHPQLQDKDFLPLKPGEPIFQLLSGEEIFYEGDVPIYPAFINEAAYYEKGVAFLKLE 299
sp|Q5M876|ACY3_RAT MEVYKNLGSVDFPRTTDGHLTGTVHSRLQDHDFEPLRPGEPIFKLFSGEDVLYEGDSVVYPLFVNEAAYYEKRVAFLKSE 299
tr|H0WH68|H0WH68_OTOGA MKAYSIVGTVDFPRTKDGHLAGTVHPRLQDQDFELLQPGAPIFQMFSGEDVLYEGESTVYPVFINEAAYYEKGIAFFQTK 299
tr|H0UYR8|H0UYR8_CAVPO MEVYKFLGCMDFPRSEDGDLAGMVHPQLQDQDFEPVQPGAPIFQLFSGEDMLYEGDSTMYPLFINEAAYYEKNIAFMKSK 298
tr|G1R3Y2|G1R3Y2_NOMLE MEAYRPVGVVDFPRTEAGNLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYPMFINEAAYYEKGVAFVQTE 299
tr|F7A210|F7A210_MACMU MEAYRPVGVMDFPRTDAGNLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYDGESTVYPVFINEAAYYEKGIAFVQTE 299
tr|G7PP64|G7PP64_MACFA MEAYRPVGVMDFPRTDAGNLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYDGESTVYPVFINEAAYYEKGIAFVQTE 299
tr|G3I3Y2|G3I3Y2_CRIGR MDIYRTLGSVDFPRTADGDLTGTVHPQLQDHDFEPLRPGEPIFKLFSGEDVLYEGNSIVYPVFINEAAYYEKNVAFLESE 299
tr|G1PIL7|G1PIL7_MYOLU LEAYRNVSSVDFPRTETGDLAGTVHPQLQ--------------------------------------------------- 247
: : :.:** *.. . :* **
sp|P45381|ACY2_HUMAN KLTL-NAKSI--RCCLH------ 313 tr|H2QBW4|H2QBW4_PANTR KLTL-NAKSI--RCCLH------ 313 tr|G3QQC1|G3QQC1_GORGO KLTL-NAKSI--RCSLH------ 313 sp|Q5R9E0|ACY2_PONAB KLTL-NAKSI--RCSLH------ 313 tr|G1S5Z4|G1S5Z4_NOMLE KLTL-NAKSI--RCSSH------ 313 tr|G7PT66|G7PT66_MACFA KLML-NAKSI--RCSVH------ 313 tr|F7DR53|F7DR53_MACMU KLML-NAKSI--RCSVH------ 313 tr|F6WMI4|F6WMI4_HORSE KLTL-NAKSI--RSSLH------ 313 sp|B1PK17|ACY2_PIG KLTL-NAKGI--HSSLH------ 313 tr|G3TAV8|G3TAV8_LOXAF KLTL-NAKSI--CSSLH------ 313 sp|P46446|ACY2_BOVIN KLTL-NANSI--RSSLH------ 313 tr|G1SPT6|G1SPT6_RABIT TLTL-NAKSI--RSSSH------ 313 tr|H0WW85|H0WW85_OTOGA KLTL-KAKSI--RSSLH------ 313 tr|G1P679|G1P679_MYOLU ILTL-KAKSI--RSSLH------ 312 tr|E2R8M6|E2R8M6_CANFA KLIL-NARSI--RSSLH------ 313 tr|G5B939|G5B939_HETGA KLTL-NAKSI--RSSFH------ 308 sp|Q9R1T5|ACY2_RAT KLTL-NAKSI--RSTLH------ 312 tr|B2KFD4|B2KFD4_MOUSE KLTL-SAKSI--RSTLH------ 312 tr|F6ZFN3|F6ZFN3_ORNAN KVTL-NAQGI--KSPCK------ 314 tr|H9H8M1|H9H8M1_MONDO KVTL-TARRL--DAAGA------ 303 tr|G3VQ32|G3VQ32_SARHA K-RTFSIPPL--ET--PAASTP- 316 sp|Q5M876|ACY3_RAT KIRI-SVPALPGLT--PSSTQTP 319 tr|H0WH68|H0WH68_OTOGA KLTF-SVPALPALT--SACTPGP 319 tr|H0UYR8|H0UYR8_CAVPO KLTV-SIPALPVLT--PNPTPLT 318 tr|G1R3Y2|G1R3Y2_NOMLE KFTF-TVPAMPALT--PDPSPAS 319 tr|F7A210|F7A210_MACMU KFTF-TVPAMPVLN--PAPSPAS 319 tr|G7PP64|G7PP64_MACFA KFTF-TVPAMPVLN--PAPSPAS 319 tr|G3I3Y2|G3I3Y2_CRIGR RIRV-SVPALPGLT--SSSTLVP 319 tr|G1PIL7|G1PIL7_MYOLU ----------------------- 247
