Structure-based mutation analysis (PKU)

Finding the right structure

As proposed we searched the UNIProt entry for our protein and then selected the entry with the highest resolution and the lowest r-Value. In our case this is 1J8U which is the protein in a complex with its cosubstrate BH4. IN the following we will only use this structure, but we also list the results we found. <figtable id="uniprotresult">

Table with results from UNIProt with r-Value inserted

Entry	Method	Resolution (Å)	r-Value	Chain	Positions	PDBsum
1DMW	X-ray	2.00	0.200	A	118-424	[»]
1J8T	X-ray	1.70	0.197	A	103-427	[»]
1J8U	X-ray	1.50	0.157	A	103-427	[»]
1KW0	X-ray	2.50	0.220	A	103-427	[»]
1LRM	X-ray	2.10	0.211	A	103-427	[»]
1MMK	X-ray	2.00	0.199	A	103-427	[»]
1MMT	X-ray	2.00	0.213	A	103-427	[»]
1PAH	X-ray	2.00	0.176	A	117-424	[»]
1TDW	X-ray	2.10	0.206	A	117-424	[»]
1TG2	X-ray	2.20	0.213	A	117-424	[»]
2PAH	X-ray	3.10	0.251	A/B	118-452	[»]
3PAH	X-ray	2.00	0.175	A	117-424	[»]
4ANP	X-ray	2.11	0.204	A	104-427	[»]
4PAH	X-ray	2.00	0.169	A	117-424	[»]
5PAH	X-ray	2.10	0.163	A	117-424	[»]
6PAH	X-ray	2.15	0.171	A	117-424	[»]

</figtable> In <xr id="uniprotresult"/> there are all results according to which we selected 1J8U to be our reference for this weeks task. The corresponding line is marked in yellow.