Difference between revisions of "Task 3: odba human Sequence-based predictions"

From Bioinformatikpedia
(reprof)
(reprof)
Line 9: Line 9:
 
reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof"
 
reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof"
 
Result:
 
Result:
  +
MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPR
 
  +
MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKE
LLHHHHHHHHHHHHLLLLHHHHHHHLLLLLLLELLLLLLL
 
  +
LLHHHHHHHHHHHHLLLLHHHHHHHLLLLLLLELLLLLLLLHLLLLLLLLLLLLLLLLHHHHHHHHLLLLLLELLLLEEEEELLLLLEELLLLLLLLLHH
 
 
  +
KVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKER
QQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYR
 
  +
HHHHHHHHHHHHHLHLHHEEELLLLLEEEEEEELLLLLLLLELLLLELLLLLEEEEEELLLLEEEELLLLHHHHHHHHHLLHLLLLLLLLLLLELLLLLL
LHLLLLLLLLLLLLLLLLHHHHHHHHLLLLLLELLLLEEE
 
 
 
  +
HFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDG
VMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILY
 
  +
EEEEELLLHHHHLHHHHHHHHHHHHLLLLEEEEEEELLLLLLLLLLLLLLEEEEELLLLEEEEEELLLEEELLLLLLLELLLLEELLLLLEEEEEEEELL
EELLLLLEELLLLLLLLLHHHHHHHHHHHHHHHLHLHHEE
 
 
 
  +
NDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERK
ESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYRE
 
  +
LLEEEEELLLHHHHHHHHHLLLLEEEEHEEEEELLLLLLLLLLHLLLLLLLLLLLLLLLLHHHHHHHHHLLLLLLHHHHHHHHHHHHHHHHHHHHHHHLL
ELLLLLEEEEEEELLLLLLLLELLLLELLLLLEEEEEELL
 
 
 
  +
PKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLDHFDK
AGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKER
 
  +
LLLLLLEEELHHHHHLHHHHHHHHHHHHHHHHHHLLLLLLLLLLL
LLEEEELLLLHHHHHHHHHLLHLLLLLLLLLLLELLLLLL
 
 
HFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGA
 
EEEEELLLHHHHLHHHHHHHHHHHHLLLLEEEEEEELLLL
 
 
ASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYR
 
LLLLLLLLLLEEEEELLLLEEEEEELLLEEELLLLLLLEL
 
 
GDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAE
 
LLLEELLLLLEEEEEEEELLLLEEEEELLLHHHHHHHHHL
 
 
NQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHP
 
LLLEEEEHEEEEELLLLLLLLLLHLLLLLLLLLLLLLLLL
 
 
ISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERK
 
HHHHHHHHHLLLLLLHHHHHHHHHHHHHHHHHHHHHHHLL
 
 
PKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPL
 
LLLLLLEEELHHHHHLHHHHHHHHHHHHHHHHHHLLLLLL
 
 
DHFDK
 
LLLLL
 
   
 
= Disorder =
 
= Disorder =

Revision as of 14:45, 12 May 2012

secondary structure

To predict secondary structure we use the following tools and compare the results:

-reprof
-psipred
-DSSP_Server

reprof

to run reprof from the command line the following command is used:

reprof -i seq.fasta

reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof" Result:

MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKE
LLHHHHHHHHHHHHLLLLHHHHHHHLLLLLLLELLLLLLLLHLLLLLLLLLLLLLLLLHHHHHHHHLLLLLLELLLLEEEEELLLLLEELLLLLLLLLHH

KVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKER
HHHHHHHHHHHHHLHLHHEEELLLLLEEEEEEELLLLLLLLELLLLELLLLLEEEEEELLLLEEEELLLLHHHHHHHHHLLHLLLLLLLLLLLELLLLLL

HFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDG
EEEEELLLHHHHLHHHHHHHHHHHHLLLLEEEEEEELLLLLLLLLLLLLLEEEEELLLLEEEEEELLLEEELLLLLLLELLLLEELLLLLEEEEEEEELL

NDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERK
LLEEEEELLLHHHHHHHHHLLLLEEEEHEEEEELLLLLLLLLLHLLLLLLLLLLLLLLLLHHHHHHHHHLLLLLLHHHHHHHHHHHHHHHHHHHHHHHLL

PKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLDHFDK
LLLLLLEEELHHHHHLHHHHHHHHHHHHHHHHHHLLLLLLLLLLL

Disorder

Transmembrane helices

Signal peptides

GO terms