Difference between revisions of "Task 3: odba human Sequence-based predictions"

From Bioinformatikpedia
(secondary structure)
(reprof)
Line 9: Line 9:
 
reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof"
 
reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof"
 
Result:
 
Result:
  +
<table border=1>
  +
<tr><td>
  +
<td>M</td><td>A</td><td>V</td><td>A</td><td>I</td><td>A</td><td>A</td><td>A</td><td>R</td><td>V</td><td>W</td><td>R</td><td>L</td><td>N</td><td>R</td><td>G</td><td>L</td><td>S</td><td>Q</td><td>A</td><td>A</td><td>L</td><td>L</td><td>L</td><td>L</td><td>R</td><td>Q</td><td>P</td><td>G</td><td>A</td><td>R</td><td>G</td><td>L</td><td>A</td><td>R</td><td>S</td><td>H</td><td>P</td><td>P</td><td>R</td></tr><tr>
  +
<td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td></tr><tr>
  +
<td>Q</td><td>Q</td><td>Q</td><td>Q</td><td>F</td><td>S</td><td>S</td><td>L</td><td>D</td><td>D</td><td>K</td><td>P</td><td>Q</td><td>F</td><td>P</td><td>G</td><td>A</td><td>S</td><td>A</td><td>E</td><td>F</td><td>I</td><td>D</td><td>K</td><td>L</td><td>E</td><td>F</td><td>I</td><td>Q</td><td>P</td><td>N</td><td>V</td><td>I</td><td>S</td><td>G</td><td>I</td><td>P</td><td>I</td><td>Y</td><td>R</td></tr><tr>
  +
<td>L</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td></tr><tr>
  +
<td>V</td><td>M</td><td>D</td><td>R</td><td>Q</td><td>G</td><td>Q</td><td>I</td><td>I</td><td>N</td><td>P</td><td>S</td><td>E</td><td>D</td><td>P</td><td>H</td><td>L</td><td>P</td><td>K</td><td>E</td><td>K</td><td>V</td><td>L</td><td>K</td><td>L</td><td>Y</td><td>K</td><td>S</td><td>M</td><td>T</td><td>L</td><td>L</td><td>N</td><td>T</td><td>M</td><td>D</td><td>R</td><td>I</td><td>L</td><td>Y</td></tr><tr>
  +
<td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>H</td><td>L</td><td>H</td><td>H</td><td>E</td><td>E</td></tr><tr>
  +
<td>E</td><td>S</td><td>Q</td><td>R</td><td>Q</td><td>G</td><td>R</td><td>I</td><td>S</td><td>F</td><td>Y</td><td>M</td><td>T</td><td>N</td><td>Y</td><td>G</td><td>E</td><td>E</td><td>G</td><td>T</td><td>H</td><td>V</td><td>G</td><td>S</td><td>A</td><td>A</td><td>A</td><td>L</td><td>D</td><td>N</td><td>T</td><td>D</td><td>L</td><td>V</td><td>F</td><td>G</td><td>Q</td><td>Y</td><td>R</td><td>E</td></tr><tr>
  +
<td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td></tr><tr>
  +
<td>A</td><td>G</td><td>V</td><td>L</td><td>M</td><td>Y</td><td>R</td><td>D</td><td>Y</td><td>P</td><td>L</td><td>E</td><td>L</td><td>F</td><td>M</td><td>A</td><td>Q</td><td>C</td><td>Y</td><td>G</td><td>N</td><td>I</td><td>S</td><td>D</td><td>L</td><td>G</td><td>K</td><td>G</td><td>R</td><td>Q</td><td>M</td><td>P</td><td>V</td><td>H</td><td>Y</td><td>G</td><td>C</td><td>K</td><td>E</td><td>R</td></tr><tr>
  +
<td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td></tr><tr>
  +
<td>H</td><td>F</td><td>V</td><td>T</td><td>I</td><td>S</td><td>S</td><td>P</td><td>L</td><td>A</td><td>T</td><td>Q</td><td>I</td><td>P</td><td>Q</td><td>A</td><td>V</td><td>G</td><td>A</td><td>A</td><td>Y</td><td>A</td><td>A</td><td>K</td><td>R</td><td>A</td><td>N</td><td>A</td><td>N</td><td>R</td><td>V</td><td>V</td><td>I</td><td>C</td><td>Y</td><td>F</td><td>G</td><td>E</td><td>G</td><td>A</td></tr><tr>
  +
<td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td></tr><tr>
  +
<td>A</td><td>S</td><td>E</td><td>G</td><td>D</td><td>A</td><td>H</td><td>A</td><td>G</td><td>F</td><td>N</td><td>F</td><td>A</td><td>A</td><td>T</td><td>L</td><td>E</td><td>C</td><td>P</td><td>I</td><td>I</td><td>F</td><td>F</td><td>C</td><td>R</td><td>N</td><td>N</td><td>G</td><td>Y</td><td>A</td><td>I</td><td>S</td><td>T</td><td>P</td><td>T</td><td>S</td><td>E</td><td>Q</td><td>Y</td><td>R</td></tr><tr>
  +
<td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>L</td></tr><tr>
  +
<td>G</td><td>D</td><td>G</td><td>I</td><td>A</td><td>A</td><td>R</td><td>G</td><td>P</td><td>G</td><td>Y</td><td>G</td><td>I</td><td>M</td><td>S</td><td>I</td><td>R</td><td>V</td><td>D</td><td>G</td><td>N</td><td>D</td><td>V</td><td>F</td><td>A</td><td>V</td><td>Y</td><td>N</td><td>A</td><td>T</td><td>K</td><td>E</td><td>A</td><td>R</td><td>R</td><td>R</td><td>A</td><td>V</td><td>A</td><td>E</td></tr><tr>
  +
<td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td></tr><tr>
  +
<td>N</td><td>Q</td><td>P</td><td>F</td><td>L</td><td>I</td><td>E</td><td>A</td><td>M</td><td>T</td><td>Y</td><td>R</td><td>I</td><td>G</td><td>H</td><td>H</td><td>S</td><td>T</td><td>S</td><td>D</td><td>D</td><td>S</td><td>S</td><td>A</td><td>Y</td><td>R</td><td>S</td><td>V</td><td>D</td><td>E</td><td>V</td><td>N</td><td>Y</td><td>W</td><td>D</td><td>K</td><td>Q</td><td>D</td><td>H</td><td>P</td></tr><tr>
  +
<td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>E</td><td>H</td><td>E</td><td>E</td><td>E</td><td>E</td><td>E</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td></tr><tr>
  +
<td>I</td><td>S</td><td>R</td><td>L</td><td>R</td><td>H</td><td>Y</td><td>L</td><td>L</td><td>S</td><td>Q</td><td>G</td><td>W</td><td>W</td><td>D</td><td>E</td><td>E</td><td>Q</td><td>E</td><td>K</td><td>A</td><td>W</td><td>R</td><td>K</td><td>Q</td><td>S</td><td>R</td><td>R</td><td>K</td><td>V</td><td>M</td><td>E</td><td>A</td><td>F</td><td>E</td><td>Q</td><td>A</td><td>E</td><td>R</td><td>K</td></tr><tr>
  +
<td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td></tr><tr>
  +
<td>P</td><td>K</td><td>P</td><td>N</td><td>P</td><td>N</td><td>L</td><td>L</td><td>F</td><td>S</td><td>D</td><td>V</td><td>Y</td><td>Q</td><td>E</td><td>M</td><td>P</td><td>A</td><td>Q</td><td>L</td><td>R</td><td>K</td><td>Q</td><td>Q</td><td>E</td><td>S</td><td>L</td><td>A</td><td>R</td><td>H</td><td>L</td><td>Q</td><td>T</td><td>Y</td><td>G</td><td>E</td><td>H</td><td>Y</td><td>P</td><td>L</td></tr><tr>
  +
<td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>E</td><td>E</td><td>E</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>H</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td><td>L</td></tr><tr>
  +
<td>D</td><td>H</td><td>F</td><td>D</td><td>K</td><td></td></table>
   
 
= Disorder =
 
= Disorder =

Revision as of 14:36, 12 May 2012

secondary structure

To predict secondary structure we use the following tools and compare the results:

-reprof
-psipred
-DSSP_Server

reprof

to run reprof from the command line the following command is used:

reprof -i seq.fasta

reprof then calculates the secondary structure prediction and provides an output file "seq.fasta.reprof" Result:

MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPR
LLHHHHHHHHHHHHLLLLHHHHHHHLLLLLLLELLLLLLL
QQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYR
LHLLLLLLLLLLLLLLLLHHHHHHHHLLLLLLELLLLEEE
VMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILY
EELLLLLEELLLLLLLLLHHHHHHHHHHHHHHHLHLHHEE
ESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYRE
ELLLLLEEEEEEELLLLLLLLELLLLELLLLLEEEEEELL
AGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKER
LLEEEELLLLHHHHHHHHHLLHLLLLLLLLLLLELLLLLL
HFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGA
EEEEELLLHHHHLHHHHHHHHHHHHLLLLEEEEEEELLLL
ASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYR
LLLLLLLLLLEEEEELLLLEEEEEELLLEEELLLLLLLEL
GDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAE
LLLEELLLLLEEEEEEEELLLLEEEEELLLHHHHHHHHHL
NQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHP
LLLEEEEHEEEEELLLLLLLLLLHLLLLLLLLLLLLLLLL
ISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERK
HHHHHHHHHLLLLLLHHHHHHHHHHHHHHHHHHHHHHHLL
PKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPL
LLLLLLEEELHHHHHLHHHHHHHHHHHHHHHHHHLLLLLL
DHFDK

Disorder

Transmembrane helices

Signal peptides

GO terms