Difference between revisions of "Canavan Task 2 - Sequence alignments"
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=== BLASTP === |
=== BLASTP === |
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| − | Run BlastP on student machines |
+ | Run BlastP on student machines with big_80 database. |
Command: |
Command: |
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-o output file |
-o output file |
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| + | We got 195 resulting sequences, out of which the last 101 have EValues below E^-15 and of which none is an Aspartoacylase. |
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| − | |||
| − | m alignment view |
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| − | v |
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| − | b |
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Revision as of 17:48, 2 May 2012
Sequence
Native ASPA sequence:
UniProt: P45381
>hsa:443 ASPA, ACY2, ASP; aspartoacylase; K01437 aspartoacylase [EC:3.5.1.15] (A) MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKK CTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTS NMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVG PQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIA AIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTK LTLNAKSIRCCLH
BLASTP
Run BlastP on student machines with big_80 database.
Command:
blastall -p blastp -d /mnt/project/pracstrucfunc12/data/big/big_80 -i P45381_wt.fasta -o blastp_p45381_wt_big80.out
-p specifies the blast type -d database -i input file -o output file
We got 195 resulting sequences, out of which the last 101 have EValues below E^-15 and of which none is an Aspartoacylase.
