Structure-based mutation analysis

General

According to the UniProt entry about HFE_HUMAN are three 3D-structures of the HFE_HUMAN available, which are listed below. We have chosen the '1A6Z' because it has the best resolution, a very good R-Value (it measures the quality of the model obtained from the crystallographic data), a pH near the physiological optimum and is as good as complete. '1DE4' has a slightly better R-Value and pH, but this PDB also includes the transferrin receptor, which we do not need and do not want in our structure. '1C42' is only a hypothetical model, so we exclude it from further research.

stereochemistrical properties of 1a6z

All stereochemistrical properties of the structure are shown in the figure to the right<ref>Lebrón JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN, Bjorkman PJ.: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor.</ref>.

PDB	Method	Resolution (Å)	Chain	R-Value	R-Free	pH
1A6Z	X-ray	2.60	A/C	0.233	0.277	6.5
1C42	model	-	A	-	-	-
1DE4	X-ray	2.80	A/D/G	0.231	0.265	8.0

Mapping

Because we have no annotation about the active site so we just visualized the mutations at the '1A6Z' structure. Secondly we are using the same mutations as in Task 6 and have therefore the same problemes with their visualization (only 7 of 10 visualizeable).

All mutations are scattered accross the protein with no affinity to some special region or secondary structure element. Mutations shown in red are near glycosylation positions and mutations in yellow are near sulfide bridges.

1A6ZA with 7 of 10 visualized mutations taken from task 6

References

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