Difference between revisions of "Sequence-based mutation analysis BCKDHA"

Revision as of 18:42, 21 June 2011

	Reference amino acid			Mutated amino acid			BLOSUM62	PAM1	PAM250	Secondary Structure
Position	Residue	Properties	Structure	Residue	Properties	Structure	BLOSUM62	PAM1	PAM250	Secondary Structure
29	G	tiny, small		E	charged, polar					C
125	Q	acidic, polar		E	charged, polar					C
166	Y	hydrophobic, aromatic, polar		N	acidic, polar, small					H
249	G	tiny, small		S	polar, small, tiny, hydroxylic					H
264	C	sulphur containing, hydrophobic, tiny, small, polar		W	hydrophobic, aromatic, polar					E
265	R	charged, positive (basic), polar		W						E
326	I			T						E
361	I			V						H
409	F			C						C
438	Y			N						C

Annotation:

H = helix

E = beta-sheet

C = coil

PsiBlast was run using the following command: blastpgp -i sequence.fasta -j 5 -d /data/blast/nr/nr -C profile.ckp

@@ Line 23: / Line 23: @@
 |125||Q||acidic, polar||||E||charged, polar|| || || ||||C
 |-
-|166||Y||hydrophobic, aromatic, polar||||N||acidic, polar, small|| || || ||||C
+|166||Y||hydrophobic, aromatic, polar||||N||acidic, polar, small|| || || ||||H
 |-
-|249||G||tiny, small||||S||polar, small, tiny, hydroxylic|| || || ||||C
+|249||G||tiny, small||||S||polar, small, tiny, hydroxylic|| || || ||||H
 |-
-|264||C||sulphur containing, hydrophobic, tiny, small, polar||||W||hydrophobic, aromatic, polar|| || || ||||C
+|264||C||sulphur containing, hydrophobic, tiny, small, polar||||W||hydrophobic, aromatic, polar|| || || ||||E
 |-
-|265||R||charged, positive (basic), polar||||W|||| || || ||||C
+|265||R||charged, positive (basic), polar||||W|||| || || ||||E
 |-
-|326||I||||||T|||| || || ||||C
+|326||I||||||T|||| || || ||||E
 |-
-|361||I||||||V|||| || || ||||C
+|361||I||||||V|||| || || ||||H
 |-
 |409||F||||||C|||| || || ||||C
@@ Line 39: / Line 39: @@
 |438||Y||||||N|||| || || ||||C
 |}
+Annotation:
+H = helix
+E = beta-sheet
+C = coil
 == PSSM ==