Difference between revisions of "Sequence-based mutation analysis BCKDHA"

Revision as of 17:12, 21 June 2011

	Reference amino acid			Mutated amino acid			BLOSUM62	PAM1	PAM250
Position	Residue	Properties	Structure	Residue	Properties	Structure	BLOSUM62	PAM1	PAM250
29	G	tiny, small		E	charged, polar
125	Q	acidic, polar		E	charged, polar
166	Y	hydrophobic, aromatic, polar		N	acidic, polar, small
249	G	tiny, small		polar, small, tiny, hydroxylic
264	C	sulphur containing, hydrophobic, tiny, small, polar		W	hydrophobic, aromatic, polar
265	R	charged, positive (basic), polar		W
326	I			T
361	I			V
409	F			C
438	Y			N

PsiBlast was run using the following command: blastpgp -i sequence.fasta -j 5 -d /data/blast/nr/nr -C profile.ckp

@@ Line 18: / Line 18: @@
 !Structure
 |-
-|29||G||||||E||||
+|29||G||tiny, small||||E||charged, polar||
 |-
-|125||Q||||||E||||
+|125||Q||acidic, polar||||E||charged, polar||
 |-
-|166||Y||||||N||||
+|166||Y||hydrophobic, aromatic, polar||||N||acidic, polar, small||
 |-
-|249||G||||||S||||
+|249||G||tiny, small||||S|polar, small, tiny, hydroxylic|||
 |-
+|264||C||sulphur containing, hydrophobic, tiny, small, polar||||W||hydrophobic, aromatic, polar||
-|264||C||||||W||||
 |-
-|265||R||||||W||||
+|265||R||charged, positive (basic), polar||||W||||
 |-
 |326||I||||||T||||
@@ Line 38: / Line 38: @@
 |438||Y||||||N||||
 |}
 == PSSM ==