Difference between revisions of "Lab Journal - Task 5 (PAH)"
From Bioinformatikpedia
(→Modeller) |
(→Modeller) |
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=== Modeller === |
=== Modeller === |
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To use Modeller we follow the [https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php/Using_Modeller_for_TASK_4 tutorial] written by the students 2011. |
To use Modeller we follow the [https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php/Using_Modeller_for_TASK_4 tutorial] written by the students 2011. |
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− | *First the target sequence must be provided into PIR-format: |
+ | *First the target sequence must be provided into PIR-format (2pah has one Lysine at the end and therefore is one aa longer than P00439): |
<code> |
<code> |
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− | >P1; |
+ | >P1;2pah<br> |
− | sequence: |
+ | sequence:2pah:::::::0.00: 0.00<br> |
MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDV<br> |
MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDV<br> |
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NLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPW<br> |
NLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPW<br> |
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QFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSE<br> |
QFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSE<br> |
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KPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQR<br> |
KPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQR<br> |
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+ | IEVLDNTQQLKILADSINSEIGILCSALQKIK* |
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− | IEVLDNTQQLKILADSINSEIGILCSALQKI* |
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</code> |
</code> |
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Revision as of 10:44, 5 June 2013
Model calculation
Modeller
To use Modeller we follow the tutorial written by the students 2011.
- First the target sequence must be provided into PIR-format (2pah has one Lysine at the end and therefore is one aa longer than P00439):
>P1;2pah
sequence:2pah:::::::0.00: 0.00
MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDV
NLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPW
FPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYM
EEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGF
RLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFA
QFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSE
KPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQR
IEVLDNTQQLKILADSINSEIGILCSALQKIK*