Difference between revisions of "Sequence Search and Multiple Sequence Alignment (PKU)"
From Bioinformatikpedia
(→Reference Sequence of PAH) |
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Create and evaluate multiple sequence alignments |
Create and evaluate multiple sequence alignments |
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+ | ==Blast== |
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+ | time blast2 -p blastp -d /mnt/project/pracstrucfunc12/data/big/big -i Dropbox/Phenylketonuria/Task1/PAH.fasta -o results_blast2_standard |
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+ | real 5m0.625s |
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+ | user 3m44.300s |
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+ | sys 0m30.580s |
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==Reference Sequence of PAH== |
==Reference Sequence of PAH== |
Revision as of 11:36, 24 April 2012
Short Task Description
Perform database searches using different search tools with the PAH protein as query
Create and evaluate multiple sequence alignments
Blast
time blast2 -p blastp -d /mnt/project/pracstrucfunc12/data/big/big -i Dropbox/Phenylketonuria/Task1/PAH.fasta -o results_blast2_standard
real 5m0.625s user 3m44.300s sys 0m30.580s
Reference Sequence of PAH
>sp|P00439|PH4H_HUMAN Phenylalanine-4-hydroxylase OS=Homo sapiens GN=PAH PE=1 SV=1 MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDV NLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPW FPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYM EEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGF RLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFA QFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSE KPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQR IEVLDNTQQLKILADSINSEIGILCSALQKI