Task homologyModelling 2011

For the sequences used in this practical, protein structures have been determined. However, in real-life projects, you often do not have structures. Therefore, we will use structure prediction methods to predict the 3D structures of our sequences. We will also check whether and how the SNPs change the predicted structures.

Theoretical background talks

We will be looking at three specific programs for homology modelling. Please prepare talks about these programs:

• Modeller
• HHpred
• Swissmodel

The talks should cover

• how the methods work behind the scenes,
• some information on their performance, strengths and weaknesses (as e.g. seen in CASP),
• a brief intro into how to call them and where to find documentation,
• some discussion how to evaluate the resulting models

Calculation of models

• Get an overview of available homologous structures based on the sequence searches and alignments.
• Divide your homologous structures into groups at
  • > 60% sequence identity
  • > 40% sequence identity
  • < 40% sequence identity
• If possible (i.e. if there are structures at that level of sequence identity) create models with each of the methods using one template from each of the groups.
  • Use the default settings of the methods, i.e. use the standard workflow and directly feed the alignments to the modelling step
  • In addition: Have a look at the alignments you use for modelling.
    • Collect sequence-based information (important residues, sequence family profiles, secondary structure prediction, etc.) to check the alignment.
    • Edit the alignment.
    • Then, proceed with modelling.
    • Document what you changed and why.
  • In addition: For modelling with Modeller: Use more than one template in one modelling step (if possible due to availability of templates).

Now, you should have quite a large number of models.

Evaluate your models

• Check the numeric evaluation of your models (scores given by the modelling programs)
• Calculate the C_alpha RMSD of the models.
• Extra diligence task: define a radius of 6 Angstrom around the catalytic centre and calculate the all atom RMSD in that region
• Discuss your results: Is any method systematically better at predicting the structure? Does this depend on the similarity of the template? ...