Difference between revisions of "Structure-based mutation analysis"
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==General== |
==General== |
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| + | According to the [http://www.uniprot.org/uniprot/Q30201#section_x-ref UniProt] entry about HFE_HUMAN are three 3D-structures of the HFE_HUMAN available, which are listed below. We have chosen the '1A6Z' because it has the best resolution, a very good R-Value and is complete. '1DE4' has a slightly better R-Value, but this PDB also includes the transferrin receptor, which we do not need and do not want in our structure. '1C42' is only a hypothetical model, so we exclude it from further research. |
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| − | In Uniprot are 3 structures to the HFE-Gen assigned, we choosed 1a6z because this structure has the best resolution with 2.6A and is complete. All stereochemistrical properties of the structure are shown in the figure to the right<ref>Lebrón JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN, Bjorkman PJ.: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor.</ref>. |
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[[Image:1a6z_properties.PNG|thumb|stereochemistrical properties of 1a6z]] |
[[Image:1a6z_properties.PNG|thumb|stereochemistrical properties of 1a6z]] |
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| + | All stereochemistrical properties of the structure are shown in the figure to the right<ref>Lebrón JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN, Bjorkman PJ.: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor.</ref>. |
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| + | {| border="1" style="text-align:center; border-spacing:0" align="center" cellpadding="3" cellspacing="3" |
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| + | |- |
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| + | !PDB |
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| + | !Method |
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| + | !Resolution (Å) |
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| + | !Chain |
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| + | !R-Value |
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| + | !R-Free |
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| + | |- |
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| + | |1A6Z||X-ray||2.60||A/C||0.233||0.277 |
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| + | |- |
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| + | |1C42||model||-||A||-||- |
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| + | |- |
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| + | |1DE4||X-ray||2.80||A/D/G||0.231||0.265 |
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| + | |} |
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==SCWRL== |
==SCWRL== |
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==Energy comparison== |
==Energy comparison== |
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Revision as of 11:22, 30 June 2011
Contents
General
According to the UniProt entry about HFE_HUMAN are three 3D-structures of the HFE_HUMAN available, which are listed below. We have chosen the '1A6Z' because it has the best resolution, a very good R-Value and is complete. '1DE4' has a slightly better R-Value, but this PDB also includes the transferrin receptor, which we do not need and do not want in our structure. '1C42' is only a hypothetical model, so we exclude it from further research.
All stereochemistrical properties of the structure are shown in the figure to the right<ref>Lebrón JA, Bennett MJ, Vaughn DE, Chirino AJ, Snow PM, Mintier GA, Feder JN, Bjorkman PJ.: Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor.</ref>.
| PDB | Method | Resolution (Å) | Chain | R-Value | R-Free |
|---|---|---|---|---|---|
| 1A6Z | X-ray | 2.60 | A/C | 0.233 | 0.277 |
| 1C42 | model | - | A | - | - |
| 1DE4 | X-ray | 2.80 | A/D/G | 0.231 | 0.265 |
SCWRL
Energy comparison
References
<references />
