Difference between revisions of "Mapping SNPs HEXA"
(→Mutations annotated only in SNP-DB) |
(→Statistical comparison of HGMD and DBSNP) |
||
Line 460: | Line 460: | ||
All in all, the barplot result agrees often with the number of triplets that encodes a certain amino acid. The amino acids that are encoded by many triplet are probably more common amino acids while amino acids that are encoded only by few triplets are rare. However this is not really true in biology but it agrees partly. Furthermore the silent mutations are more common in amino acids that were encoded by many triplets which is logical and can be explained well. Besides the results are not really significant because they are very small. For a really statistic evidence more informations are necessary. However, this barplot gives a good overview for the amino acid mutation rate for this special case. |
All in all, the barplot result agrees often with the number of triplets that encodes a certain amino acid. The amino acids that are encoded by many triplet are probably more common amino acids while amino acids that are encoded only by few triplets are rare. However this is not really true in biology but it agrees partly. Furthermore the silent mutations are more common in amino acids that were encoded by many triplets which is logical and can be explained well. Besides the results are not really significant because they are very small. For a really statistic evidence more informations are necessary. However, this barplot gives a good overview for the amino acid mutation rate for this special case. |
||
− | [[Image:barplot_aa.png|center|1200px|Figure |
+ | [[Image:barplot_aa.png|center|1200px|Figure 2: Barplot for the frequency of a certain amino acid mutation for the different tables]] |
+ | |||
+ | |||
+ | [[Image:heatmap.png|center|1200px|Figure 1: Heatmap for the amino acid exchange for all non-silent mutations]] |
Revision as of 16:38, 18 June 2011
Contents
Methods
First of all, we had to parse the HGMD database and the DB-SNP database.
HGMD
We logged us in and searched for Tay-Sachs diseases and chose one entry of HEXA. In our case there were two entries with identical content. We only looked at the missense/nonsense mutations, which are 68 annotated in HGMD. We just copied the webpage in a textfile and than wrote a short parser, which parse the codon change, amino acid change and codon number.
DBSNP
It was more complicated to parse the DBSNP output, than the output of HGMD. First of all, we search for HEXA in this database and chose only the SNPs which occur in human. We used the grapical output and again copied and pasted the page in a textfile. An entry in DBSNP has following structure: First of all there is the name of the SNP. Next the sequence and the graphical representation of the sequence. In the next line, there are the allel origin, the clinical relevance and last the annotations of the mutation. We parsed the SNP-id. If there was an NP entry on the line with the annotations, there was a detailed description on which position which amino acid is changed. Than we used this annotation. If we could not find such an annotation we used the NM or NR annotation. Both of them describe the mutation at the nucleotide sequence. We used the position and divided it by 3, so therefore, we know the codon position and the position of the nucleotide exchange in the codon (1, 2 or 3). We used the sequence to get the triplet of this codon and than we mapped the original triplet and the mutated triplet to amino acids. Therefore, it was possible to annotate the position, codon position and the mutations of this position, which we wrote in our tables.
Comparison of mutations in HGMD and DBSNP
For the comparison of the mutations in HGMD and DBSNP we made table with some importent informations. First of all we extraced the DBSNP-id which makes it easy to look after this SNP if necessary. Next we looked up the codonposition which displays where the mutation takes place in the sequence. Furthermore we also extraced the mutation position which corresponds to the position in the triplet where the mutation takes place. The next two entries displays the mutations for the amino acids and codon which means it shows detailed which amino acid is replaced by another and how the codon is changed. Afterwards we create descriptive representation for the mutation by showing the sequence and coloring the correspondig kind of mutation. Following tables and descriptive visualisations are for different cases which are described below. Furthermore, a detailed summary of the different cases and a comparision of them can be seen below at [statistical comparison of HGMD and DBSNP].
Mutations annotated in both databases
First of all we compared the mutations which are annotated in both databases. These mutations are not silent and cause all the phenotype, which is known as Tay-Sachs disease. The reason for that is that HGMD contains no silent mutations. Here we found 33 annotated mutations where some of them take place in different mutationpositions in the same triplet. Besides, in some case two different named mutation take places at the same codon position.
SNP-DB Identifier | Codonposition | Mutationposition | Amino Acids | Codons |
rs121907964 | 26 | 3 | Trp -> TER | TGGc -> TGA |
rs121907979 | 39 | 2 | Leu -> Arg | CTT -> CGT |
rs121907975 | 127 | 1 | Leu -> Phe | aCTC -> TTC |
2 | Leu -> Arg | CTC -> CGC | ||
rs121907962 | 137 | 1 | Arg -> TER | cCGA -> TGA |
rs121907972 | 170 | 1 | Arg -> Trp | cCGG -> TGG |
rs121907957 | 2 | Arg -> Gln | CGG -> CAG | |
rs28941770 | 178 | 2 | Arg -> His | CGC -> CAC |
2 | Arg -> Leu | CGC -> CTC | ||
rs121907953 | 1 | Arg -> Cys | tCGC -> TGC | |
rs121907969 | 180 | 3 | Tyr -> TER | TACc -> TAG |
rs28941771 | 1 | Tyr -> His | tTAC -> CAC | |
rs121907973 | 197 | 2 | Lys -> Thr | AAA -> ACA |
rs1800429 | 200 | 1 | Val -> Met | cGTG -> ATG |
rs121907976 | 204 | 2 | His -> Arg | CAT -> CGT |
rs121907961 | 210 | 2 | Ser -> Phe | TCC -> TTC |
rs121907974 | 211 | 2 | Phe -> Ser | TTC -> TCC |
rs121907970 | 247 | 1 | Arg -> Trp | aCGG -> TGG |
rs121907959 | 250 | 1 | Gly -> Ser | gGGT -> AGT |
2 | Gly -> Asp | GGT -> GAT | ||
2 | Gly -> Val | GGT -> GTT | ||
rs121907971 | 258 | 1 | Asp -> His | tGAC -> CAC |
rs121907954 | 269 | 1 | Gly -> Ser | aGGT -> AGT |
2 | Gly -> Asp | GGT -> GAT | ||
rs121907977 | 301 | 2 | Met -> Arg | ATG -> AGG |
rs121907967 | 329 | 2 | Trp -> TER | TGG -> TAG |
rs121907963 | 393 | 1 | Arg -> TER | gCGA -> TGA |
rs121907958 | 420 | 3 | Trp -> Cys | TGGt -> TGC |
3 | Trp -> Cys | TGGt -> TGT | ||
rs28940871 | 451 | 1 | Leu -> Val | tCTG -> GTG |
rs121907978 | 454 | 2 | Gly -> Asp | GGT -> GAT |
1 | Gly -> Ser | tGGT -> AGT | ||
rs121907981 | 474 | 3 | Trp -> Cys | TGGc -> TGC |
rs121907952 | 482 | 1 | Glu -> Lys | cGAA -> AAA |
rs121907968 | 485 | 1 | Trp -> Arg | gTGG -> CGG |
rs121907966 | 499 | 1 | Arg -> Cys | aCGT -> TGT |
rs121907956 | 2 | Arg -> His | CGT -> CAT | |
rs28942071 | 504 | 1 | Arg -> Cys | cCGC -> TGC |
rs121907955 | 2 | Arg -> His | CGC -> CAC | |
rs4777502 | 506 | 3 | Glu -> Asp | GAA -> GAC |
3 | Glu -> Asp | GAA -> GAT |
Graphical representation:
The graphical representation shows at which position a certain mutation takes places. In this case only non-silent mutations are marked. The reason is that in HGMD only non-silent mutations are annotated and therefore the results which agree in both databases are also non-silent. Furthermore, there are no amino acids which are wrong annotated.
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
MTSSRLWFSLLLAAAFAGRATALWP!PQNFQTSDQRYVRYPNNFQFQYDVSSAAQPGCSVLD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLS
QCLFLSETVWGAL!GLETFSQLVWKSAEGTFFINKTEIEDFPRFPHWGLLLDTSHH!LPLS
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
SILDTLDVMAYNTLNMFHWRLVDDPFSPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
ARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFL
AWLRSIRVLAEFHTPGHTLSWGPSIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFRSTFFL
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
EVSSVFPDFYLHLGGDEVDFTC!KSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYG
KGYVVWQEVFDNKVKIQPDTIIQVW!EDIPVNYMKELELVTKAGFRALLSAPCYLNRISYG
PDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
PDWKDFYIVEPLAFEGTPEQKAVVIDGEACMWGEYVDNTNLVPRLCPRAGAVAKRLRSNKL
TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT TSDLTFAYECLSHFCCDLLRRGVQAQPLNVGFCEQEFEQT Non-silent mutation Silent mutation Wrong AA in mutation annotation
Mutations annotated only in HGMD
We also looked for mutations which are annotated only in HGMD, but we did not found any of them.
Mutations annotated only in SNP-DB
Here we listed all mutations which are annotated only in the DBSNP and which are not silent. Some of these mutations have a high detailed NP annotation while others are not annotated in such a detailed way. Therefore, we had to map these mutations. The detailed list of the mutations, which we mapped can be found [here]. Finally, 8 non-silent mutations could be found which have only one possible mutationposition and codonposition.
SNP-DB Identifier | Codonposition | Mutationposition | Amino Acids | Codons |
rs4777505 | 29 | 2 | Asn -> Ser | AAC -> AGC |
rs61731240 | 179 | 1 | His -> Asp | CAT -> GAT |
rs3743230 | 208 | 1 | Asn -> Asp | AAC -> GAC |
rs61747114 | 248 | 1 | Leu -> Phe | CTT -> TTT |
rs1054374 | 293 | 2 | Ser -> Ile | AGT -> ATT |
rs1800430 | 399 | 1 | Asn -> Asp | AAC -> GAC |
rs1800431 | 436 | 1 | Ile -> Val | ATA -> GTA |
rs121907982 | 456 | 2 | Tyr -> Ser | TAT -> TCT |
Graphical representation:
The following visualisation displays the corresponding mutations which are only in DBSNP and non-silent. Therefore there is of course no silent mutation marked while some wrong annotated amino acids exist. This could be explained, because there exist some different sequences of this protein, depending on which sequence assembly is used. Therefore, if DBSNP uses another sequence than we, it is possible that there are some other amino acids in the sequence. It is not possible to say which sequence is the right one. Therefore, we decided to color wrong annotated amino acids blue, so it is possible for the reader to see on one look that there is a difference.
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
MTSSRLWFSLLLAAAFAGRATALWPWPQSFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLS
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRDYLPLS
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
ARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFL
ARFRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPILNNTYEFMSTFFL
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVDYMKELELVTKAGFRALLSAPWYLNRISYG
PDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
PDWKDFYVVEPLAFEGTPEQKALVIGGSACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT Non-silent mutation Silent mutation Wrong AA in mutation annotation
Silent Mutations
HGMD contains no silent mutations which means that the silent mutations are extracted from DBSNP. This silent mutation do not cause change of the phenotype which means that the nucleotide exchange results in the same amino acid.
One problem is that these mutations are badly annotated in the DBSNP which means we had to prepare the found results in addition. Therefore we first rotated the found codons, because the original codon has to encode for the amino acid which occur in the protein sequence.
Therefore we used the codon with a mutation at position one, position two and position three. Next, we also reversed them and created the complemetary sequence for both.
The detailed result can be seen [here]
If we found more than one nucleotide combination which codes for the same amino acid in the protein sequence and if these are silent mutations as well, we listed them all in the following table. Otherwise, if there do not exist any other possible mutation for this postition, we also listed the mutations which are not silent.
Here are the results which displays all combinations that are possible:
SPN-DB Identifier | Codonposition | Mutationposition | Amino Acids | Codons | translation |
rs1800428 | 3 | 3 | Ser -> Ser | AGC -> AGT | Forward |
rs11551324 | 109 | 3 | Thr -> Thr | ACC -> ACT | forward |
rs28942072 | 324 | 3 | Val -> Val | GTT -> GGA | Forward |
rs28942072 | Val -> Val | GTC -> GTT | Forward | ||
rs34085965 | 446 | 1 | Pro -> Pro | CCT -> CCC | complemantry reverse |
rs4777502 | 506 | 3 | Glu -> Glu | GAG -> GAA | Forward |
Graphical representation:
In this graphical representation the silent mutations which are only in DBSNP are displayed.
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLS
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLS
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
ARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFL
ARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFL
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYG
PDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
PDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT Non-silent mutation Silent mutation Wrong AA in mutation annotation
Summary
Graphical representation:
Here we combined all graphical representations of the different cases. This means this visualisation displays all possible mutations which are found in HGMD and DBSNP. Therefore we can see non-silent mutations as well as silent mutations. Furthermore the wrong annotated amino acids are marked as well.
MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLD
MTSSRLWFSLLLAAAFAGRATALWP!PQSFQTSDQRYVRYPNNFQFQYDVSSAAQPGCSVLD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
EAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDD
QCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLS
QCLFLSETVWGAL!GLETFSQLVWKSAEGTFFINKTEIEDFPRFPHWGLLLDTSHD!LPLS
SILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
SILDTLDVMAYNTLNMFHWRLVDDPFSPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEY
ARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFL
AWFRSIRVLAEFHTPGHTLSWGPSIPGLLTPCYSGSEPSGTFGPVNPILNNTYEFRSTFFL
EVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
EVSSVFPDFYLHLGGDEVDFTC!KSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYG
KGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYG
KGYVVWQEVFDNKVKIQPDTIIQVW!EDIPVDYMKELELVTKAGFRALLSAPCYLNRISYG
PDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKL
PDWKDFYVVEPLAFEGTPEQKAVVIDGSACMWGEYVDNTNLVPRLCPRAGAVAKRLRSNKL
TSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT TSDLTFAYECLSHFCCELLRRGVQAQPLNVGFCEQEFEQT Non-silent mutation Silent mutation Wrong AA in mutation annotation
Statistical comparison of HGMD and DBSNP
For the analysis of the two different database results we decided to do some statistical comparison.
First of all we compared the different resulting tables (see above) according to their mutationposition in the triplet. Therefore we created a barplot which shows the precentage of the frequency for the corresponding mutationposition.
The first three bars show the case of the overlapping results of both databases. One can see that there are as much mutations at the first postion as at the second position. The occurance of a mutation on the third position deviates from the others which means it is much rarer. The reason for this ist that the database HGMD contains no silent mutations which means that the overlap of both databases do not contain them as well. The third position of a triplet often causes a silent mutation and there for only a few mutations on the third position result in an amino acid change. Therefore, this explains why the third position is as rare while the other positions are equal frequent.
The second case displays the position frequency of the corresponding mutations which are only resulting in DBSNP. Here one can see that mutations at position one are more frequent than at position two and that there are no mutations at position three. The resulting mutations only for DBSNP are not silent which can explain why there is no mutation at position three: mutations at the third position of a triplet are very often silent. Besides there is probably no special reason why the first position is more common than the second one for a mutation.
The third case shows the resulting silent mutations of DBSNP. Here the most frequent mutation position is the third one while the other ones are same common. This is the opposite behaviour comparing to the other cases and has a similar explantion: mutations at the third position of a triplet often result in a silent mutation where contrary silent mutations at the other position are very rare.
The last case represents the total distribution of the mutationpositions. Here the first position is the most common for a mutation followed by the second position which is almost same common. The third position is the least frequent one. This is the expected result corresponding to the other three cases: the first and the second position are almost always the most frequent ones and the third the rarest. In the third case there is an exception which is the reason why the difference is not so high in the total distribution.
In summary, the barplot of the different tables/cases correspond to the expectation and can be all explained logically.
As a next step we looked up which amino acid mutates most often for each table. Therefore we create a barplot where the frequency for a mutation of a certain amino acid is displayed for each table. The different colors correspond to the differnt tables and the total distribution. Furthermore we ploted not the absolute values, but the relative ratio of the amino acid exchange within one table (in percent).
Looking at the overlaping result of both database we can see that almost every amino acid mutation occures. The only amino acids which do not mutate are Alanine, Asparagine, Cystein, Glutamine and Threonine. Three of these amino acid (Ala, Cys, Gln) do not occure in the other tables as well. One possible reason is that this amino acid were encoded only by very little possible triplets which means that they probably occur less often. Asparagine has probably the same reason which means that only few triplets encodes it. Threonine does probably never mutate by accident, because it can be encoded by an higher number of triplets. The amino acids that mutate most common in the overlapping result of both databases are Arginine and Glycine. A possible reason for this is that Arginine can be encoded by many possible triplets as well as Glycine which has as result that they are probably more common amino acids.
Looking at the DBSNP result which were not in HGMD we can see that there are many amino acids which do not mutate. A reason for this is that the number of found mutations which are only in SNPDB and which are not silent is very low and therefore not really significant. The most common mutated amino acid here is Asparagine. A possible reason can be that this amino acid is encoded by less triplets and in relation to the other tables it occurs here very often by chance.
For the silent mutations we also extract the amino acid where a nucleotide exchange takes places. Most strikingly are Serine and Valine which have the highest percentage for silent mutations. This can be explained by the fact that these amino acids were encoded by a high number of different triplets. The reason is that a silent mutation is more usually when a amino acid is encoded by many triplets.
At last we ploted the total result of all tables together. In this case the most commonst amino acid exchange is for Arginine followed by Glycine, Serine and Tryptophan. This corresponds to the overlapping results of both databases, because it has highest number of results. The only excpetion is Serine which mutates a lot in the other two cases. Furthermore these result agrees also with the explanation that these amino acids excepted Tryptophan were encoded by many different triplets. This means that they occur probably more often in sequences. Tryptophan is probably occuring so often by chance, because it is encoded only by one specific triplet. The rest of the amino acid mutation rates correspond mostly the result of both databases as well.
All in all, the barplot result agrees often with the number of triplets that encodes a certain amino acid. The amino acids that are encoded by many triplet are probably more common amino acids while amino acids that are encoded only by few triplets are rare. However this is not really true in biology but it agrees partly. Furthermore the silent mutations are more common in amino acids that were encoded by many triplets which is logical and can be explained well. Besides the results are not really significant because they are very small. For a really statistic evidence more informations are necessary. However, this barplot gives a good overview for the amino acid mutation rate for this special case.