Difference between revisions of "Task 8: Sequence-based mutation analysis"
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| || Arg67His || aliphatic, basic polar, delocalised positive charge || aromatic ring, basic polar, mainly neutral charge || [[File:arg67his.png|center|thumb|250px| Arg67His mutation in the center with arginine in yellow and histidine in red.]] || C (loop) || 0 || 2 || -1|| 33% || 1% || -1 || 100% || 0% || |
| || Arg67His || aliphatic, basic polar, delocalised positive charge || aromatic ring, basic polar, mainly neutral charge || [[File:arg67his.png|center|thumb|250px| Arg67His mutation in the center with arginine in yellow and histidine in red.]] || C (loop) || 0 || 2 || -1|| 33% || 1% || -1 || 100% || 0% || |
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− | | || Met97Ile || sulfur containing, nonpolar, neutral charge || hydrophobic, nonpolar, neutral charge || [[File:met97ile.png|center|thumb|250px| Met97Ile mutation with methoinine in yellow and isoleucine in red.]] || H (helix) || 1 || 2 || 0 |
+ | | || Met97Ile || sulfur containing, nonpolar, neutral charge || hydrophobic, nonpolar, neutral charge || [[File:met97ile.png|center|thumb|250px| Met97Ile mutation with methoinine in yellow and isoleucine in red.]] || H (helix) || 1 || 2 || 0 || 5% || 5% || 0 || 23% || 4% || |
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| || Asn130Ser || polar, neutral charge|| polar, neutral charge || [[File:asn130ser.png|center|thumb|250px| Asn130Ser mutation with asparagine in yellow and serine in red.]] || C (loop) || 1 || 1 || -1 || 10% || 5% || || || || |
| || Asn130Ser || polar, neutral charge|| polar, neutral charge || [[File:asn130ser.png|center|thumb|250px| Asn130Ser mutation with asparagine in yellow and serine in red.]] || C (loop) || 1 || 1 || -1 || 10% || 5% || || || || |
Revision as of 17:06, 24 August 2013
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Mutation selection
<figtable id="mutations">
mutations | ||
---|---|---|
nucleotide change | amino acid change | |
Val53Met | ||
His63Asp | ||
Arg67His | ||
Met97Ile | ||
Asn130Ser | ||
Glu168Gln | ||
Leu183Pro | ||
Thr217Ile | ||
Cys282Tyr | ||
Arg330Met |
</figtable>
Mutation analysis results
<figtable id="summary">
reference | mutation | pyhsicochemical properties | strucural properties | conservation | consensus | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
from | to | pymol visualisation | secondary structure | BLOSSUM62 | PAM250 | PSSM score | occurence WT | occurence mutant | MSA score | occurence WT | occurence mutant | |||
Val53Met | brached chain, hydrophobic, nonpolar, neutral charge | sulfur containing, nonpolar, neutral charge | E (beta sheet) | 1 | 2 | 1 | 47% | 3% | -1 | 7% | 0% | |||
His63Asp | aromatic ring, basic polar, mainly neutral charge | acidic polar, negative charge | C (loop) | 1 | 2 | -3 | 2% | 2% | -1 | 26% | 0 | |||
Arg67His | aliphatic, basic polar, delocalised positive charge | aromatic ring, basic polar, mainly neutral charge | C (loop) | 0 | 2 | -1 | 33% | 1% | -1 | 100% | 0% | |||
Met97Ile | sulfur containing, nonpolar, neutral charge | hydrophobic, nonpolar, neutral charge | H (helix) | 1 | 2 | 0 | 5% | 5% | 0 | 23% | 4% | |||
Asn130Ser | polar, neutral charge | polar, neutral charge | C (loop) | 1 | 1 | -1 | 10% | 5% | ||||||
Glu168Gln | acidic polar, negative charge | polar, neutral charge | H (helix) | 2 | 2 | -1 | 7% | 2% | ||||||
Leu183Pro | branched chain, hydrophobic, nonpolar, neutral charge | nonpolar, neutral charge | H (helix) | -3 | -3 | -4 | 45% | 1% | ||||||
Thr217Ile | polar, neutral charge | hydrophobic, nonpolar, neutral charge | C (loop) | -1 | 0 | -3 | 6% | 1% | ||||||
Cys282Tyr | thiol side chain, nonpolar, neutral charge | polar, neutral charge | E (beta sheet) | -2 | 0 | -5 | 94% | 1% | ||||||
Arg330Met | aliphatic, basic polar, delocalised positive charge | sulfur containing, nonpolar, neutral charge | Could not be visualized because this resdiue is not contained in the the pdb structure. | - | -1 | 0 | -3 | 28% | 0% |
</figtable>
All results from the mutation analysis are summarised in <xr id="summary"/>. Physicochemical properties are specified as characteristics of the aa, side chain polarity and charge. The BLOSUM62 and PAM250 were used as substitution matrices. A score around 0 indicates that the aa substitution is neutral. A score below 0 indicates that the substitiution is not conserved and thus probably has a negative effect on the protein. A score above 0 denotes a substitution that has no effect on the protein.
The PSSM contains a score for each position in the sequence and each possible aa. A positive score indicates that the aa substitution is more frequent in the alignment as would be expected by change and a negative score indicates that the substitution occurs less frequent than expected. Large positive scores thus indicate that this position is highly conserved and that it is important for the protein function.
A MSA with the 100 homologous mammalian sequences with the smalles E-value was generated, as well as a MSA for all homologous sequences. <xr id="summary"/> only shows the values from the MSA with 100 sequences. The PSSMs for both alignments can be found in the Lab journal task 8.
Val53Met
The main effect of the change from valine to methonine is mainly due to the structure, because both aa are nonpolar and neutral. Methionine is linear and valine has a branched structure. As can be seen in the picture, this could lead to clashes with the alpha helix above the methionine. Also, valine is hydrophob whereas methionine is not.
His63Asp
This mutation is could be disease causing, because the basic aa histidine is replaced by the acidic aspartic acid. Besides, histidine is mainly neutral whereas the aspartic acid is negatively charged. But the pymol picture shows that residue 63 is located in a surface loop. Since both aa are not hydrophob, the implication of this aa exchange for the function of the protein is therefor not as severe as if this mutation would be located in the interior of the protein.
Arg67His
Arginine is able to form multiple H-bonds due to its delocalised positive charge and histidine is also able to form H-bonds. Both aa are basic polar. Thus, the main difference between arginine and histidine is the positive and neutral charge and that histidine contains an aromatic ring whereas arginine is aliphatic. The pymol visualisation of the mutation shows that the mutation is located in a surface loop and since both aa are not hydrophob, we think that the effect of the mutation should not be too severe.
Met97Ile
The main difference between methinine and isolecine is that methinine contains a sulfur atom and isoleucine not. Both aa are nonpolar and neutral charged, but isoleucine is hydropbob and methionine not due to its sulfur. The residue 97 is part of an alpha helix. The Psiblast PSSM shows that this position is not conserved, because all 20 aa have an occurence of 5%.
Asn130Ser
Asparagine can form H-bonds with the backbone and serine is also able to form H-bonds. The physicochemical proberties of both aa are very similar, therefore we think, that this mutations is probably not disease causing. This opinion is also supported by the 3D visualisation of the mutation which shows that the asparagine is located at the end of a beta sheet on the surface of the proteins. The isoleucine at this position does not disrupt the local structure.
Glu168Gln
Glutatimc acid as well as glutamie are both polar, but glutamic acid has a negative charge whereas glutamine is neutral. The only difference between the two aa is that glutamine has an NH group where glutamic acid contains an OH group. Consequently there is not big difference in 3D structure but only in physiochemical properties. This can also be seen in the pymol picture. The substitution matrices both have a score of 2 for this mutations, which indicates that the mutation does probably not effect the protein much.
Leu183Pro
Proline is a unique aa in that it disrupts secondary structure. Since the leucine is located inside an alpha helix (see picture), the mutation to proline disrupts the helix and thus most probably leads to a disturbed function of the protein and causes the disease. This is also indicated by the negative score (-3) of both substitution matrices. But the Proline is not the worst possible substitution, because several aa lead to a score of -4 in the BLOSUM62 and the OAM250 even gives a -6 for the substitution with cysteine.
Thr217Ile
Threonine is polar whereas isoleucine is nonpolar and thus hydrophob. As the threonine is located on the surface of the protein, as can be seen in the pymol visualisation, a hydrophob residue at this position might lead to a structural change of the protein.
Cys282Tyr
Cystein can form an disulfide bonds with another cystein residue. Disulfide bonds are very important for the protein structure and stability. The picture shows that the cystein is part of a beta sheet and located in the interior of the protein. The polar threonine thus disrupts possible disulfide bonds and the protein structure. This mutation is therefore likely disease causing. The BLOSUM62 matrix has a score of -2 that supports an effect of the SNP, but the PAM250 matrix has a 0 that says that the mutation is neutral.
Arg330Met
Arginine and methionine have very different physicochemical properties. Arginine is basic polar and positive charged whereas methionine is nonpolar and uncharged. This mutation could therefore als have an effect on the protein function.
<figtable id="predictions">
reference | mutation | consensus | prediction programms | truth | |||
---|---|---|---|---|---|---|---|
SIFT | Polyphen2 | MutationTaster | SNAP |
</figtable>