Difference between revisions of "Tastk 2: Alignments"
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== Sequence Searches == |
== Sequence Searches == |
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| − | TODO: |
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| − | what blast parameters should be used? |
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Blast:<br> |
Blast:<br> |
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| + | blastall -i /mnt/home/student/betza/data/hfe.fasta -d /mnt/project/pracstrucfunc13/data/big/big_80 -O /mnt/home/student/betza/task2/hfe_blast.txt -p blastp -v 1000 |
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| + | HHblits parameter:<br> |
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| + | hhblits -i /mnt/home/student/betza/data/hfe.fasta -d /mnt/project/rost_db/data/hhblits/uniprot20_02Sep11 -o /mnt/home/student/betza/task2/hfe.hhr -oa3m /mnt/home/student/betza/task2/hfe.a3m -oalis /mnt/home/student/betza/task2/hfe -ohhm /mnt/home/student/betza/task2/hfe.hhm -Z 1000 |
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| + | increase number of lines in summary hit list to 1000 in blast and HHblits. |
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| − | HHblits parameter:<br> |
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| − | hhblits -i /mnt/home/student/betza/data/hfe.fasta -d /mnt/project/rost_db/data/hhblits/uniprot20_02Sep11 -o /mnt/home/student/betza/task2/hfe.hhr -oa3m /mnt/home/student/betza/task2/hfe.a3m -oalis /mnt/home/student/betza/task2/hfe -ohhm /mnt/home/student/betza/task2/hfe.hhm |
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Latest revision as of 23:39, 3 May 2013
Sequence Searches
Blast:
blastall -i /mnt/home/student/betza/data/hfe.fasta -d /mnt/project/pracstrucfunc13/data/big/big_80 -O /mnt/home/student/betza/task2/hfe_blast.txt -p blastp -v 1000
HHblits parameter:
hhblits -i /mnt/home/student/betza/data/hfe.fasta -d /mnt/project/rost_db/data/hhblits/uniprot20_02Sep11 -o /mnt/home/student/betza/task2/hfe.hhr -oa3m /mnt/home/student/betza/task2/hfe.a3m -oalis /mnt/home/student/betza/task2/hfe -ohhm /mnt/home/student/betza/task2/hfe.hhm -Z 1000
increase number of lines in summary hit list to 1000 in blast and HHblits.
