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https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_08_-_Sequence-based_mutation_analysis&diff=38956
Gaucher Disease: Task 08 - Sequence-based mutation analysis
2013-09-30T22:29:44Z
<p>Kalemanovm: /* Mutation Analysis */</p>
<hr />
<div>[[Gaucher_Disease:_Task_08_-_LabJournal|Lab journal]]<br />
<br />
==Mutation Set==<br />
<figtable id="sele"><br />
{| border="1" cellpadding="6" cellspacing="0" align="center" <br />
|-<br />
! colspan="6" style="background:#adceff;" | Mutations<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | mRNA<br />
! colspan="3" style="background:#adceff;" | Protein<br />
|-<br />
! style="background:#efefef;" | Reference<br />
! style="background:#efefef;" | Sequence Position<br />
! style="background:#efefef;" | Codon change <br />
! style="background:#efefef;" | Codon Number<br />
! style="background:#efefef;" | Amino Acid change<br />
! style="background:#efefef;" | One letter code<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234] || 656 || AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || 77 || Ser ⇒ Arg || S77R <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673] || 847 || A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || 141 || Asn ⇒ Ser || N141S <br />
|-<br />
| CM880035 ||- ||C<span style="color:#FF0040">'''G'''</span>G ⇒ C<span style="color:#FF0040">'''A'''</span>G || 159 || Arg ⇒ Gln || R159Q <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374591570 rs374591570] || 1062 || <span style="color:#FF0040">'''C'''</span>TC ⇒ <span style="color:#FF0040">'''T'''</span>TC || 213 || Leu ⇒ Phe || L213F <br />
|-<br />
| CM992894 ||- || G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A||241 || Gly ⇒ Glu || G241E <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=371083513 rs371083513] || 1470 || <span style="color:#FF0040">'''G'''</span>TA ⇒ <span style="color:#FF0040">'''A'''</span>TA ||349 || Val ⇒ Ile || V349I <br />
|-<br />
| CM960697 ||- || A<span style="color:#FF0040">'''C'''</span>G ⇒ A<span style="color:#FF0040">'''T'''</span>G || 408|| Thr ⇒ Met || T408M <br />
|-<br />
| CM880036 ||- || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || 409 || Asn ⇒ Ser || N409S <br />
|- <br />
| CM870010 ||- || C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || 483 || Leu ⇒ Pro || L483P <br />
|-<br />
| CM057072 ||- || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C|| 501|| Asn ⇒ Ser || N501S <br />
|-<br />
|}<br />
<center><small>'''<caption>''' Information about 10 randomly selected mutations for glucocerebrosidase taken from HGMD (CM...) and dbSNP (rs...).</caption></small></center><br />
</figtable><br />
<br />
==Mutation Analysis==<br />
In our analysis we looked closer to the amino acid properties and their changing characteristics by mutation. We analyzed the structural difference between wild type (WT) and mutation. We also considered their secondary structure and distinguished between helix (H), sheet (E) and loop (C). We also took two different substitution matrices into account, BLOSUM62 and PAM250. '''P'''oint '''A'''ccepted '''M'''utation matrix has only positive integer values as scores and is not symmetric. The score reflects the probability of an amino acid to mutate into another. In contrast the '''BLO'''cks '''SU'''bstitution '''M'''atrix has also negative integers and is symmetric. A positive score indicates that a substitution occurs more than random. While a score of 0 shows that the substitution occurs randomly, a negative one points to a mutation less frequent than a random mutation. In case one of our selected mutations has the worst possible substitution score for this amino acid we highlighted the score red in <xr id="ana"/>.<br />
To consider also evolutionary information we created different PSSM matrices. These position specific scoring matrices are based on alignments. Just as BLOSUM, the PSSM has positive and negative integer values as scores. A positive value shows that the substitution occurs more often than expected. Critical functional residues, like active site residues, have high positive scores. One PSSM was created with a Psi-BLAST search. The other one is based on an alignment consisting of all mammalian homologous sequences.<br />
<br />
<br />
<figtable id="ana"><br />
{| border="1" cellpadding="13" cellspacing="0" align="center" <br />
|-<br />
! colspan="13" style="background:#adceff;" | Mutation Analysis<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Changes of Physiochemical Properties<br />
! colspan="2" style="background:#adceff;" | Structural Properties<br />
! colspan="7" style="background:#adceff;" | Conservation<br />
! colspan="1" style="background:#adceff;" | Effect<br />
|-<br />
! style="background:#efefef;" | Mutation<br />
! style="background:#efefef;" | From<br />
! style="background:#efefef;" | To<br />
! style="background:#efefef;" | Pymol Visualization<br />
! style="background:#efefef;" | Secondary Structure<br />
! style="background:#efefef;" | BLOSUM62 score<br />
! style="background:#efefef;" | PAM250 score<br />
! style="background:#efefef;" | PSSM score<br />
! style="background:#efefef;" | PSSM WT frequency<br />
! style="background:#efefef;" | PSSM mutant frequency<br />
! style="background:#efefef;" | MSA WT frequency<br />
! style="background:#efefef;" | MSA mutant frequency<br />
! style="background:#efefef;" |<br />
|-<br />
| S77R ||polar, neutral charge, sulfur-containing || polar, positive charge, basic ||[[Image:mut_S38R.png|thumb|250x250px| Mutation of serine (blue) to arginine (orange) on position 77. ]] || E||-1 ||6||1||11%||9%|| 64%||2%|| slightly negative <br />
|-<br />
| N141S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing ||[[Image:mut_N102S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 141. ]] ||H ||1||5||0|| 10%||7%||55%||3%|| neutral<br />
|-<br />
| R159Q || polar, positive charge, basic || polar, neutral charge, acidic||[[Image:mut_R120Q.png|thumb|250x250px| Mutation of arginine (blue) to glutamine (orange) on position 159.]] || E|| 1||5||-4||83%||0%||86%|| 0%|| negative<br />
|-<br />
| L213F || nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, aromatic, hydrophobic||[[Image:mut_L174F.png|thumb|250x250px| Mutation of leucine (blue) to phenylalanine (orange) on position 213.]] || E||0||13||3||22% ||13%||100%||0%||slightly negative <br />
|-<br />
| G241E || nonpolar, neutral charge, aliphatic || polar, negative charge, acidic||[[Image:mut_G202E.png|thumb|250x250px| Mutation of glycine (blue) to glutamic acid (orange) on position 241.]]||C || -2 ||9||-1|| 10%||3%||83%||0%||slightly negative <br />
|-<br />
| V349I ||nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, aliphatic, hydrophobic ||[[Image:mut_V310I.png|thumb|250x250px| Mutation of valine (blue) to isoleucine (orange) on position 349. ]] ||E ||3 || <span style="color:#FF0040">4</span>||0||14%|| 5%||97%||3%||neutral<br />
|-<br />
| T408M || polar, neutral charge, hydroxyl-containing || nonpolar, neutral charge, sulfur-containing||[[Image:mut_T369M.png|thumb|250x250px| Mutation of threonine (blue) to methionine (orange) on position 408. ]] || H|| -1 ||5||-1|| 4%||2%||82%||0%||neutral<br />
|-<br />
| N409S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing|| [[Image:mut_N370S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 409.]] ||H ||1||5||1|| 10%||9%||76%||2%||slightly negative <br />
|- <br />
| L483P || nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, cyclic||[[Image:mut_L444P.png|thumb|250x250px| Mutation of serine (blue) to arginine (orange) on position 483]] ||E ||-3||5||-3|| 29%||1%||100%||0%|| high negative<br />
|-<br />
| N501S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing||[[Image:mut_N462S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 501.]] ||E ||1||5||-2||87%||3%||86%||1%|| negative<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Analysis of the chosen mutations of <xr id="sele"/> in the field of their properties, secondary structure and conservation. The secondary structure can be classified as helix (H), sheet (E) and loop (C). In case a mutations is the worst possible substitution for this amino acid, the substitution matrix score is colored red. The effect of the mutation is based on our analysis. A detailed description can be read below.</caption></small></center><br />
</figtable><br />
<br />
Based on the analysis summed up in <xr id="ana"/> we interpreted our mutations:<br />
<br />
'''S77R : ''' The biggest change happens in the secondary structure. While serine has a short and neutral side chain, arginine shows a much longer positive side chain, that probably causes a clash with the flexible loops of the environment. Additional to the change in its polarity the residue switches from sulfur containing to basic. This could destabilize its secondary structure, as the parallel located sheet may be not fixed anymore to the sheet of the residue. The PSSM show only no high frequency for the WT as well as the mutant. With scores of -1 and 6, the substitution matrices identifies the point mutation as expectable. We think the only effect comes from the structural change and has a slightly negative effect.<br />
<br />
'''N141S : '''<br />
The mutation causes no change in its charge and polarity. The affected residue is located in a helix on the protein surface, which let us assume that no effect may occur (neutral). The substitution matrices as well as the PSSM score confirm us to this opinion, as the scores (1[BLOSUM], 5[PAM] and 0[PSSM]) indicate the mutation as nearly random. Also the PSSM frequency tells us that the mutation is rare (7% and 3%) and the WT not very distinct (10% and 55%).<br />
<br />
'''R159Q : '''<br />
The substitution changes the residue from basic and positive charged into a acidic residue without charge. The mutant has a much longer side chain which extends deep into the protein. This structural characteristics as well as the great pH change let us assume that a clash or effect on the structure around this amino acid cannot be avoided. The scores, especially the evolutionary based position specific score, affirms us in our assumption. In the end, the frequency of the WT (83% and 86%) as well as the absence of the mutant in the MSAs, leave us no doubt of the mutation severeness. <br />
<br />
'''L213F : '''<br />
The amino acid is located in a sheet and turns from aliphatic to aromatic. Although, this is a great structural change, there seem to be no clashes or other influences on the neighborhood. Both substitution matrices indicate this to occur on random. Even though, the PSSM shows an mutation appearance more than random and the mutation can be seen in 13% of the alignment sequences, the MSA of homologous sequences consists only of leucine at this position. However, we think that the mutation may be neutral, but uncommon between the homologous sequences. As we are not quite sure, we defined it as possible damaging.<br />
<br />
'''G241E : '''<br />
There is great change in the physicochemical properties caused by the mutation from glycine to glutamine acid, especially for the charge and pH. The residue appears in a loop on the protein surface. Its side chain does not extend into the protein. That is why the property change will not have an effect on the protein structure. The substitution scores deviate from each other. While BLOSUM shows an occurrence less than random, PAM indicates this mutation to happen more frequent. Also the different MSAs show frequencies that make the data interpretation difficult. Due to the combination of the structural and the property characteristics, we would assume the mutation as neutral. But because of the remaining data, we are not sure and declare it as slightly damaging. <br />
<br />
'''V349I : '''<br />
The mutation from valine to isoleucine makes no difference in the properties. Also, both branch chain amino acids differ only slightly in their structure. The PAM declares the substitution as rare, as the score of 4 is the worst for valine, but occurs for several amino acids. Based on evolutionary information the PSSM score defines the mutation as random. This is not surprising concerning their similarity. In the MSAs both amino acids appear at position 349, but valine more often. Based on this observation, we are convinced that this is a neutral mutation between similar amino acids, which occurs once in a while.<br />
<br />
'''T408M : '''<br />
The mutant and the WT differ in their polarity. While threonine is hydroxyl containing, methionine has a sulfur atom in its structure. The side chain causes no clashes with other residues. This substitution happens little less than random. Both amino acids are rare at position 408 in the PSSM alignment. Considering only homologous sequences the WT occurs way more often (82%). The mutant is not observable. We interpret this data as a neutral mutation.<br />
<br />
'''N409S : '''<br />
The mutation causes no difference in charge and polarity. It occurs in a helix and lies close to a sheet, which lets us assume that they stabilize each other. However, the mutation may not destabilize the structure very much. The substitution happens nearly random. In the PSSM MSA, the mutant appears nearly as often as the WT (difference 1%). But the frequencies in the homologous alignment differ much more (difference 73%). We think that this mutation has a minimal effect on the protein that can cause a slight form of the disease, but definitely not deadly. <br />
<br />
'''L483P : '''<br />
There seem to be no great changes in the pysicochemical properties except the cyclic characteristic of proline. The residue is located at the end of a sheet and may be not important for the structure stabilization, but it is obvious that the ring clashes with following residues of the adjoining loop which can be severe for the structure. Compared to lysine, proline has one amino group less which can also cause to a destabilization. Especially the BLOSUM and PSSM scores (both -3) show an rare occurrence of the mutation. The frequency balance between mutation and wild type is very dissimilar (29%>>1%). By looking at the evolutionary information of the homologs, we can see that the WT is present in all sequences. This can give as a hint to the severeness of the mutation. The reason for never showing up as well as a rare appearance, can be that the mutation causes not only a disease but death. All information about this mutation let us identify a high negative effect. <br />
<br />
'''N501S : '''<br />
The pysicochemical properties have been described before for mutation N409. The amino acid is located at the beginning of a beta sheet. The side chain does not clash with residues in its neighborhood. The substitution matrix scores show that the mutation appears more than randomly. But the PSSM score shows a lower probability for the mutation. Also the alignments show that the WT occurs much more often (87% and 86%) than the mutant. This frequency tells us that the mutation happens seldom. We suppose this as an observation of selection pressure. So, we assume that the mutation is disease causing.<br />
<br />
==Comparison of different approaches==<br />
First of all we interpreted our collected data from analysis of <xr id="ana"/>. After that we run several predictors of mutation effects. All these results are summarized in <xr id="app"/>. Then we reassessed our analysis by considering the prediction results. In the end we validated our new interpretation (consensus in <xr id="app"/>) against the databases dbSNP and HGMD. In two cases, both databases had contrary mutation information. While HGMD identifies them as disease causing, dbSNP classifies the two mutations as non disease causing. That is why we marked them as possibly damaging.<br />
For three mutations (N141S, R159Q, N501S) all predictions, our interpretations, and the validation totally agree with each other. So, we can say for sure that the mutation of asparagine to serine on position 141 has no effect. However, the mutation on position 159 from arginine to glutamine is defintely disease causing as well as the SNP at position 501 from asparagine to serine. <br />
In the end, we classified the mutation N409S as disease causing, because the score of SIFT is marginal to disease causing, which would answer our assumption of a slight disease. <br />
<br />
<figtable id="app"><br />
{| border="1" cellpadding="8" cellspacing="0" align="center" <br />
|-<br />
! colspan="8" style="background:#adceff;" | Summary of different prediction approaches<br />
|-<br />
! style="background:#efefef;" | Mutation<br />
! style="background:#efefef;" | Analysis of <xr id="ana"/><br />
! style="background:#efefef;" | SIFT <br />
! style="background:#efefef;" | Polyphen2 (HumDiv)<br />
! style="background:#efefef;" | MutationTaster<br />
! style="background:#efefef;" | SNAP<br />
! style="background:#efefef;" | Consensus<br />
! style="background:#efefef;" | Validation<br />
|-<br />
|S77R <br />
! style="background:#F4FA58;" | pcd<br />
! style="background:#2EFE64;" | 0.37 <br />
! style="background:#2EFE64;" | 0.17<br />
! style="background:#FA5858;" | 90%<br />
! style="background:#2EFE64;" | 2<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|N141S<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | 0.15<br />
! style="background:#2EFE64;" | 0.01<br />
! style="background:#2EFE64;" | 83%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|R159Q<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 1<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#FA5858;" | 7<br />
! style="background:#FA5858;" | dc <br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|L213F<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.79<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#F4FA58;" | HGMD[dc] vs dbSNP[ndc]<br />
|-<br />
|G241E<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#FA5858;" | 0.01 <br />
! style="background:#FA5858;" | 0.89<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#F4FA58;" | HGMD[dc] vs dbSNP[ndc]<br />
|-<br />
|V349I<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | 0.25 <br />
! style="background:#2EFE64;" | 0.12<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|T408M<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#FA5858;" | 0.03 <br />
! style="background:#2EFE64;" | 0.11<br />
! style="background:#2EFE64;" | 60%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|N409S<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#2EFE64;" | 0.05 <br />
! style="background:#2EFE64;" | 0.23<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|L483P<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.85<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 1<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|N501S<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.98<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#FA5858;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Information about selected mutations from different predictors of amino acid substitution effects as well as our own interpretation based on our data of the previous exercises of the task 08. The consensus is our opinion of the effect based on the predictions and our analysis. We distinguish between three different kind of effects: <span style="background:#FA5858">'''disease causing (dc)'''</span>, <span style="background:#F4FA58">'''possibly damaging (pdc)'''</span> and <span style="background:#2EFE64">'''non-disease causing (ndc)'''</span>. The prediction scores are colored according to this. The validation contains the information of the databases (HGMD, dbSNP).</caption></small></center><br />
</figtable></div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_09_-_Structure-based_mutation_analysis&diff=38953
Gaucher Disease: Task 09 - Structure-based mutation analysis
2013-09-29T23:52:40Z
<p>Kalemanovm: /* 2. Visualization of the mutations to work with */</p>
<hr />
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<br />
==Preparation==<br />
<br />
===1. Choice of a structure to work with===<br />
<br />
We chose the structure '''2V3E, chain B''', which has the following properties:<br />
<br />
<figtable id="2V3E"><br />
{|class="colBasic2"<br />
!PDB-ID || Resolution (Å) || Chain || Covered residues (UniProt seq.) || Missing residues (ATOM seq.) || Covered residues (ATOM seq.) || R-Value(obs.) || R-Free || pH || Temperature (K)<br />
|-<br />
|[http://www.pdb.org/pdb/files/2V3E.pdb 2V3E] || 2.0 || A/B || 40-536 (92.7%) || A: 31, (498-503), B: (-1), (498-503) || A: -1-30, 32-497, B: '''0-497''' || 0.163 || 0.220 || '''7.5''' || 100<br />
|}<br />
<center><small>'''<caption>''' Properties of 2V3E, chain B, the chosen reference structure of GBA sequence P04062.</caption></small></center><br />
</figtable><br />
<br />
For more information about other candidates and the missing residues, see the [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].<br />
<br />
===2. Visualization of the mutations to work with===<br />
<br />
We selected the following five mutation from the mutations selected in for this task:<br />
<br />
<figtable id="mutations"><br />
{|class="colBasic2"<br />
! Reference || Codon number (UniProt) || Codon number (PDB) || Codon change || Amino acid change || Polarity || Charge (pH) || Disease causing?<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234]|| 77 || 38 || AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || Ser ⇒ Arg (S77R) ||polar ⇒ polar || neutral ⇒ positive || FALSE<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673]|| 141 || 102 || A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || Asn ⇒ Ser (N141S) || polar ⇒ polar || neutral ⇒ neutral || FALSE <br />
|-<br />
| CM992894|| 241 || 202 || G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A|| Gly ⇒ Glu (G241E) || nonpolar ⇒ polar || neutral ⇒ negative || TRUE<br />
|-<br />
| CM880036|| 409 || 370 || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || Asn ⇒ Ser (N409S) || polar ⇒ polar || neutral ⇒ neutral || TRUE <br />
|- <br />
| CM870010|| 483 || 444 || C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || Leu ⇒ Pro (L483P) || nonpolar ⇒ nonpolar || neutral ⇒ neutral || TRUE<br />
|}<br />
<center><small>'''<caption>''' Selected mutations of GBA sequence P04062. Mapping of the UniProt positions onto the PDB ATOM sequence is given. </caption></small></center><br />
</figtable><br />
<br />
The following figure visualizes the five residues we are going to mutate on the reference structure, 2V3E, chain B.<br />
<br />
<figure id="pymol_mutations" ><br />
<gallery widths=550px heights=300px perrow="2"><br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-labeled.png|The five residues to be mutated mapped onto the structure 2V3E, chain B. Those residues are represented as green (not disease causing S77R and N141S) and red (disease causing G241E, N409S and L483P) spheres. The ligands bound to the structure at three binding sites during its resolution (BMA, FUC, NAG and NND) are represented as spheres in different shades of orange. Neither of the five residues lies in the proximity of one of the binding sites. <br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-other_side-labeled.png| Same as the first figure, only view from the other side of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of five residues of the structure 2V3E, chain B, we are going to mutate as described in Table 2.</caption></small><br />
</figure><br />
<br />
As can be seen on the image, none of the residues to be mutated lies in the proximity of one of the three binding sites. (See also the second subfigures of the figure galleries in the next subsection for close up on the residues and their hydrogen bonds.) However, four of the residues lie within a secondary structure element (beta sheet or helix) and one - Glycine 241 - in a turn<br />
near a helix. This implies that exchange of these residues with others with different functional groups, polarity and charge could lead to destruction of some hydrogen bonds within or between the secondary structures (e.g. Asparagine 141). This might lead to structural changes and even to destruction of the secondary structures or important blocks of secondary structure elements. Moreover, an exchange with a side chain of a bigger size might lead to clashed with proximate residues (e.g. with the loop near the Serine 77).<br />
<br />
===3. Creation of mutated structures===<br />
<br />
We used SQWRL4 to create the five mutated structures. (See [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].) The mutated residues in comparison to the native residues, the hydrogen of the mutants and possible clashes are shown in the following figures.<br />
<br />
====S77R====<br />
<figure id="S77R" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_S38R_sticks.png| Comparison of wild type (wt) Serine 77 (magenta) and mutant Argenine (orange). The mutated residue has a longer side chain.<br />
File:2V3E_B_S38_hb.png| Serine 77 (PDB 38) is colored magenta, its chain is in the stick representation and all the backbone residues are in the cartoon representation (the same for the following subfigures). Serine 77 lies in the middle of a beta strand flanked by two other strands in an anti-parallel order. It forms two hydrogen bonds with a residue in a neighboring strand.<br />
File:2V3E_B_mutant_38R_hb.png| Hydrogen bonds formed by the mutant Argenine 77 remain conserved, as they are formed by the main chain.<br />
File:2V3E_B_mutant_38R_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Argenine 77 (orange) shows, that its side chain does not clash with the neighboring loop (gray spheres).<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''S77R''' (PDB 38) of the structure 2V3E, chain B, with SCWRL.</caption></small><br />
</figure><br />
<br />
According to this images, we would also predict the mutation S77R as not disease causing.<br />
<br />
====N141S====<br />
<figure id="N141S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N102S_sticks.png| Comparison of wild type (wt) Asparagine 141 (magenta) and mutant Serine (orange). The mutated side chain is a little shorter and points towards another direction.<br />
File:2V3E_B_N102_hb.png| Asparagine 141 (PDB 102) is located in a helix and forms four hydrogen contacts: two with the main-chain carboxyl- and amino-groups with two helix amino acids (one in each direction) and two with the side chain. One of the contacts of the Asparagine side chain also binds to the same residue in the helix as the Asparagine amino-group binds. The second hydrogen bond of the Asparagine side chain is formed with the side chain of a residue in the neighboring helix. Maybe this bond plays a stabilizing role between the two helices. <br />
File:2V3E_B_mutant_102S_hb_2diff.png| Hydrogen bonds formed by the mutant Serine 141. A contact that was formed between the native Asparagine side chain and a side chain pointing from a neighboring helix (showed in sticks) is missing, as well as a second contact with a residue in the same helix. Instead, the mutated Serine side chain forms a contact with another residue in the same helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_missing_contact.png| Spheres representation of the mutant Serine and the missing contact with the neighboring helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Serine and a proximate loop (gray spheres) - there is no clash.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N141S''' (PDB 102) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
The mutation N141S is benign, therefore the missing stabilizing contact between the two helices is probably not so important.<br />
<br />
====G241E====<br />
<figure id="G241E"><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_G202E_sticks.png| Comparison of wild type (wt) Glycine 241 (magenta) and mutant Glutamate (orange). The mutated residue has a long side chain.<br />
File:2V3E_B_G202_hb.png| Glycine 241 (PDB 202) lies in a turn in the proximity of a helix end and in front of an another helix. Its amino-group forms a hydrogen bond to a residue of the latter helix. <br />
File:2V3E_B_mutant_202E_hb.png| The mutant Glutamate 241 maintains the same hydrogen bond (formed by the main chain) with a residue in a neighboring helix. The residue lies on the surface and the bigger side chain points to the outside of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''G241E''' (PDB 202) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
As no changes in the hydrogen bonds or clashes etc. can be noticed, we would predict the mutation G241E as benign. However, it is a disease causing mutation. Maybe the effect is caused by the polarity and negative charge of the mutant Glutamate, instead of the nonpolar and neutral Glycine.<br />
<br />
====N409S====<br />
<figure id="N409S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N370S_sticks.png| Comparison of wild type (wt) Asparagine 409 (magenta) and mutant Serine (orange). The mutated side chain is a little bit smaller, but points towards the same direction.<br />
File:2V3E_B_N370_hb.png| Asparagine 409 (PDB 370) is located in a helix. It builds five hydrogen bonds: four with the main chain and one with the side chain. Two of the main chain contacts are formed by the carboxyl-group: one with a helix residue and one with a residue located in a proximate beta-strand beginning, the two binding partners are separated by a short turn. Two other main chain hydrogen bonds are built by the amino-group and two carboxyl-groups of an amino acid in the helix (from the other side). The fifth bond is formed between the side chain amino-group and one of the caroboxy-groups of the last mentioned helix residue.<br />
File:2V3E_B_mutant_370S_hb.png| Hydrogen bonds formed by the mutant Serine 409. The five hydrogen bonds described in the figure on the left with the Asparagine 409 contacts are conserved.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N409S''' (PDB 370) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
Due to the fact that all hydrogen bonds remain conserved in the mutant and that no clashes etc. could be detected, we would predict the mutation N409S as non-effect. Moreover, the both amino acids, Asparagine and Serine, are both polar and neutral. Nevertheless, this mutation is annotated as disease causing. <!-- Do I oversee something?--><br />
<br />
====L483P====<br />
<figure id="L483P" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_L444P_sticks.png| Comparison of wild type (wt) Leucine 483 (magenta) and mutant Proline (orange). The mutant Proline is a cycle amino acid, which has very different properties than the hydrophobic Leucine. Is leads to a shift in the main chain conformation "backwards", so that the beginning of the neighboring strand is shifted. <br />
File:2V3E_B_L444_hb.png| Leucine 483 (PDB 444) lies at the very beginning of a beta strand. There is another beta strand in the proximity on the right, which is followed by three others, forming a beta sheet. However, the Leucine does not form any contact with the neighboring strand, as its side chain is hydrophobic and points in another direction. The Leucine forms an interesting hydrogen bond with its amino-group to the side chain of a residue located in the neighboring turn. After this loop another beta-strand follows.<br />
File:2V3E_B_mutant_444P_hb_sticks_missing_contact.png| The mutant Proline 483 does not form a hydrogen bond with the residue in the loop, like the Leucine main chain amino-group does (see the corresponding figure). The reason for this is that Proline lacks a free amino-group due to the cycle formation.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''L483P''' (PDB 444) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
We would predict the mutation L483P as having an effect - and so it is according to annotations.<br />
<br />
==Energy comparisons==<br />
[[Gaucher Disease: Task 09 - Lab Journal|Lab journal]]<br />
<br />
===foldX===<br />
<br />
We applied foldX for the five mutations to predict new structures with energies. <br />
<br />
<figtable id="scwrl_foldx"><br />
{| class="colBasic2"<br />
! || colspan="3" | SCWRL || colspan="5" | FoldX<br />
|-<br />
! Mutant || Energy || Mutant-WT || Predicted effect || Energy Mutant || Energy WT || Mutant-WT || Predicted effect || Observed effect<br />
|-<br />
| WT || 386.356 || || || || || || || <br />
|-<br />
| S77R || 386.706 || 0.350 || FALSE || 20.90 || 23.25 || -2.35 || FALSE || FALSE<br />
|-<br />
| N141S || 390.966 || 4.610 || TRUE || 28.81 || 28.84 || -0.03 || FALSE || FALSE<br />
|-<br />
| G241E || 394.319 || 7.963 || TRUE || 32.30 || 29.31 || 2.99 || TRUE || TRUE<br />
|-<br />
| N409S || 391.73 || 5.374 || TRUE || 29.08 || 27.77 || 1.31 || TRUE || TRUE<br />
|-<br />
| L483P || 424.715 || 38.359 || TRUE || 38.40 || 35.55 || 2.85 || TRUE || TRUE<br />
|}<br />
<center><small>'''<caption>''' Energies of the WT structure and the five mutant structures calculated by SQWRL and foldX. FoldX minimizes the energy of the WT structure for each mutation. The energy difference between each mutant and the respective WT structure was calculated. If it is (significantly) positive, the mutation is regarded as effect causing. The observed effect of the mutation is given for comparison. </caption></small></center><br />
</figtable><br />
<br />
We decided to regard all differences between the mutant and WT energies higher than 1 as significant. SCWRL identifies the three disease causing mutations as such. However, it also predicts one not disease causing mutation (N141S) as effect causing, thought with the smallest positive difference (higher 1) of 4.61. Interestingly, the most prevalent Gaucher disease mutation according to OMIM, L483P (L444P in OMIM), has a highest energy change of 38.359. FoldX makes a correct prediction in all cases, the two benign mutations even have a small negative energy change. However, the disease causing mutations have all only a small energy increase (1.31 - 2.99). In foldX the mutation L483P does not have the highest energy increase, in the contrary to SCWRL.<br />
<br />
Next, we superimposed the SCWRL and foldX structures in Pymol and compared them in the following figures.<br />
<br />
<figure id="scwrl_vs_foldx" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutant_38R.png| Mutation S77R. There is almost no difference in the conformation. However, foldX predicts two additional hydrogen bonds between the Argenine side chain: one to a residues in a proximate loop and the second is a big Tyrosine side chain from the same beta strand.<br />
File:2V3E_B_mutant_102S.png| Mutation N141S. There is almost no difference in the conformation and the same hydrogen bonds are predicted.<br />
File:2V3E_B_mutant_202E.png| Mutation G241E. In foldX the conformation of the Glutamate side chain is a little bit different, but it has no meaning, as it lies on the outside of the protein. Also the binding partner side chain has a little bit different angle, so that two hydrogen bonds, instead of only one in SCWRL, are formed.<br />
File:2V3E_B_mutant_370S.png| Mutation N409S. SCWRL and foldX agree in the conformation of the mutated and the binding residues, including the hydrogen bonds formed between them, apart from tiny shifts.<br />
File:2V3E_B_mutant_444P.png| Mutation L483P. Also here SCWRL and foldX predict nearly the same conformation of the Proline (and the residue which forms a hydrogen bond in WT with the Leucine).<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL (gray protein, orange mutated residue, yellow polar contacts) and foldX (lime protein, cyan mutated residue, dark cyan polar contacts).</caption></small><br />
</figure><br />
<br />
===Minimise===<br />
<br />
We applied minimise for all mutant structures produced by SCWRL and foldX and the WT structure. We used the output of one minimisation for another run as input 5 times for each structure. The <xr id="minimise"/> summarizes the resulting energies:<br />
<br />
<br />
<figtable id="minimise"><br />
{| class="colBasic2"<br />
! Method || Mutation || Round 1 || Round 2 || Round 3 || Round 4 || Round 5<br />
|-<br />
! - <br />
|| WT || style="background-color: #FFD700"| -12360.0 || -11661.9 || -12079.7 || -11636.9 || -11630.6<br />
|-<br />
! rowspan="5" | SCWRL<br />
|| S77R || -3482.3 || style="background-color: #FFD700"| -12111.1 || -11724.2 || -11509.3 || -11260.2<br />
|-<br />
|| N141S || -3593.4 || style="background-color: #FFD700"| -12199.7 || -11787.7 || -11568.1 || -11328.3<br />
|-<br />
|| G241E || -3548.4 || style="background-color: #FFD700"| -12163.9 || -11792.4 || -11593.1 || -11381.2<br />
|-<br />
|| N409S || -3631.0 || style="background-color: #FFD700"| -12214.2 || -11801.9 || -11594.4 || -11347.0<br />
|-<br />
|| L483P || -3469.4 || style="background-color: #FFD700"| -11900.1 || -11610.9 || -11665.8 || -11330.6<br />
|-<br />
!rowspan="5" | foldX <br />
|| S77R || style="background-color: #FFD700"| -12297.0 || -11892.1 || -12037.2 || -11707.4 || -11703.1<br />
|-<br />
|| N141S || style="background-color: #FFD700"| -12359.7 || -11961.2 || -11983.4 || -11550.9 || -11515.9<br />
|-<br />
|| G241E || style="background-color: #FFD700"| -12356.6 || -11944.8 || -12103.6 || -11782.5 || -11764.0<br />
|-<br />
|| N409S || style="background-color: #FFD700"| -12389.9 || -11966.0 || -12117.7 || -11845.4 || -11808.9<br />
|-<br />
|| L483P || style="background-color: #FFD700"| -12335.4 || -11925.2 || -12096.9 || -11771.4 || -11771.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Energies produced by each of five consecutive runs of the program '''minimise''', applied on the WT structure and the five mutant structures calculated by SQWRL and foldX. The lowest energies for each structure are marked gold.</caption></small></center><br />
</figtable><br />
<br />
For the WT, the lowest energy is reached after the first iteration of minimise. This is because the structure is already correct and therefore optimal. Interestingly, the lowest energy of the SQWRL mutants is reached after two minimise iterations, whereas for the foldX mutants the minimal energy is reached already after the first iteration. This may be explained by the fact that foldX already performs a minimisation step. Moreover, foldX minimal energies are always lower, than those calculated by SCWRL.<br />
<br />
Another interesting observation is that after reaching the lowest energy in the second minimise iterations in the WT and the foldX mutants, the energy rises constantly in the consequent runs. This probably means that the minimal local energy is reached in the second iteration and then the computation of the gradient at the minimal point can only lead to a higher energy value, maybe to another local minimum with a higher energy. A different pattern happens during the minimisation of the SCWRL mutants: after reaching the local minimal energy already after the first iteration, in the second iteration the energy rises, however it falls a little again in the third iteration, thought not into the same local minimum as in the first iteration. This indicates that a second "suboptimal" local minimum is found. After the forth iteration the energy rises again. <br />
<br />
We compared the mutant structures in Pymol again after the minimise runs reaching the best energies: after the second round for SQWRL and the first round for foldX (<xr id="minimise"/>).<br />
<br />
<figure id="minimise" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_S38R_minimised-scwrl_vs_foldx.png| Mutation S77R.<br />
File:2V3E_B_N102S_minimised-scwrl_vs_foldx.png| Mutation N141S.<br />
File:2V3E_B_G202E_minimised-scwrl_vs_foldx.png| Mutation G241E.<br />
File:2V3E_B_N370S_minimised-scwrl_vs_foldx.png| Mutation N409S. <br />
File:2V3E_B_L444P_minimised-scwrl_vs_foldx.png| Mutation L483P.<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL after two minimise iterations (gray protein, orange mutated residue) and foldX after one minimise iteration (lime protein, cyan mutated residue). There is almost no difference in the conformations of all the mutations. </caption></small><br />
</figure><br />
<br />
To conclude, SQWRL and foldX are both pretty good tools for calculating the energy difference and the structural change of a protein after a point mutation. There is almost no difference in the resulting structures between the two programs, in particular the predicted conformations of the mutated residues and their environments are very similar.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_09_-_Structure-based_mutation_analysis&diff=38952
Gaucher Disease: Task 09 - Structure-based mutation analysis
2013-09-29T22:40:54Z
<p>Kalemanovm: /* 2. Visualization of the mutations to work with */</p>
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==Preparation==<br />
<br />
===1. Choice of a structure to work with===<br />
<br />
We chose the structure '''2V3E, chain B''', which has the following properties:<br />
<br />
<figtable id="2V3E"><br />
{|class="colBasic2"<br />
!PDB-ID || Resolution (Å) || Chain || Covered residues (UniProt seq.) || Missing residues (ATOM seq.) || Covered residues (ATOM seq.) || R-Value(obs.) || R-Free || pH || Temperature (K)<br />
|-<br />
|[http://www.pdb.org/pdb/files/2V3E.pdb 2V3E] || 2.0 || A/B || 40-536 (92.7%) || A: 31, (498-503), B: (-1), (498-503) || A: -1-30, 32-497, B: '''0-497''' || 0.163 || 0.220 || '''7.5''' || 100<br />
|}<br />
<center><small>'''<caption>''' Properties of 2V3E, chain B, the chosen reference structure of GBA sequence P04062.</caption></small></center><br />
</figtable><br />
<br />
For more information about other candidates and the missing residues, see the [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].<br />
<br />
===2. Visualization of the mutations to work with===<br />
<br />
We selected the following five mutation from the mutations selected in for this task:<br />
<br />
<figtable id="mutations"><br />
{|class="colBasic2"<br />
! Reference || Codon Number (UniProt) || Codon Number (PDB) || Codon change || Amino Acid change || Polarity || Charge (pH) || Disease causing?<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234]|| 77 || 38 || AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || Ser ⇒ Arg (S77R) ||polar ⇒ polar || neutral ⇒ positive || FALSE<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673]|| 141 || 102 || A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || Asn ⇒ Ser (N141S) || polar ⇒ polar || neutral ⇒ neutral || FALSE <br />
|-<br />
| CM992894|| 241 || 202 || G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A|| Gly ⇒ Glu (G241E) || nonpolar ⇒ polar || neutral ⇒ negative || TRUE<br />
|-<br />
| CM880036|| 409 || 370 || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || Asn ⇒ Ser (N409S) || polar ⇒ polar || neutral ⇒ neutral || TRUE <br />
|- <br />
| CM870010|| 483 || 444 || C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || Leu ⇒ Pro (L483P) || nonpolar ⇒ nonpolar || neutral ⇒ neutral || TRUE<br />
|}<br />
<center><small>'''<caption>''' Selected mutations of GBA sequence P04062. Mapping of the UniProt positions onto the PDB ATOM sequence is given. </caption></small></center><br />
</figtable><br />
<br />
The following figure visualizes the five residues we are going to mutate on the reference structure, 2V3E, chain B.<br />
<br />
<figure id="pymol_mutations" ><br />
<gallery widths=550px heights=300px perrow="2"><br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-labeled.png|The five residues to be mutated mapped onto the structure 2V3E, chain B. Those residues are represented as green (not disease causing S77R and N141S) and red (disease causing G241E, N409S and L483P) spheres. The ligands bound to the structure at three binding sites during its resolution (BMA, FUC, NAG and NND) are represented as spheres in different shades of orange. Neither of the five residues lies in the proximity of one of the binding sites. <br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-other_side-labeled.png| Same as the first figure, only view from the other side of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of five residues of the structure 2V3E, chain B, we are going to mutate as described in Table 2.</caption></small><br />
</figure><br />
<br />
As can be seen on the image, none of the residues to be mutated lies in the proximity of one of the three binding sites. (See also the second subfigures of the figure galleries in the next subsection for close up on the residues and their hydrogen bonds.) However, four of the residues lie within a secondary structure element (beta sheet or helix) and one - Glycine 241 - in a turn<br />
near a helix. This implies that exchange of these residues with others with different functional groups, polarity and charge could lead to destruction of some hydrogen bonds within or between the secondary structures (e.g. Asparagine 141). This might lead to structural changes and even to destruction of the secondary structures or important blocks of secondary structure elements. Moreover, an exchange with a side chain of a bigger size might lead to clashed with proximate residues (e.g. with the loop near the Serine 77).<br />
<br />
===3. Creation of mutated structures===<br />
<br />
We used SQWRL4 to create the five mutated structures. (See [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].) The mutated residues in comparison to the native residues, the hydrogen of the mutants and possible clashes are shown in the following figures.<br />
<br />
====S77R====<br />
<figure id="S77R" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_S38R_sticks.png| Comparison of wild type (wt) Serine 77 (magenta) and mutant Argenine (orange). The mutated residue has a longer side chain.<br />
File:2V3E_B_S38_hb.png| Serine 77 (PDB 38) is colored magenta, its chain is in the stick representation and all the backbone residues are in the cartoon representation (the same for the following subfigures). Serine 77 lies in the middle of a beta strand flanked by two other strands in an anti-parallel order. It forms two hydrogen bonds with a residue in a neighboring strand.<br />
File:2V3E_B_mutant_38R_hb.png| Hydrogen bonds formed by the mutant Argenine 77 remain conserved, as they are formed by the main chain.<br />
File:2V3E_B_mutant_38R_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Argenine 77 (orange) shows, that its side chain does not clash with the neighboring loop (gray spheres).<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''S77R''' (PDB 38) of the structure 2V3E, chain B, with SCWRL.</caption></small><br />
</figure><br />
<br />
According to this images, we would also predict the mutation S77R as not disease causing.<br />
<br />
====N141S====<br />
<figure id="N141S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N102S_sticks.png| Comparison of wild type (wt) Asparagine 141 (magenta) and mutant Serine (orange). The mutated side chain is a little shorter and points towards another direction.<br />
File:2V3E_B_N102_hb.png| Asparagine 141 (PDB 102) is located in a helix and forms four hydrogen contacts: two with the main-chain carboxyl- and amino-groups with two helix amino acids (one in each direction) and two with the side chain. One of the contacts of the Asparagine side chain also binds to the same residue in the helix as the Asparagine amino-group binds. The second hydrogen bond of the Asparagine side chain is formed with the side chain of a residue in the neighboring helix. Maybe this bond plays a stabilizing role between the two helices. <br />
File:2V3E_B_mutant_102S_hb_2diff.png| Hydrogen bonds formed by the mutant Serine 141. A contact that was formed between the native Asparagine side chain and a side chain pointing from a neighboring helix (showed in sticks) is missing, as well as a second contact with a residue in the same helix. Instead, the mutated Serine side chain forms a contact with another residue in the same helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_missing_contact.png| Spheres representation of the mutant Serine and the missing contact with the neighboring helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Serine and a proximate loop (gray spheres) - there is no clash.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N141S''' (PDB 102) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
The mutation N141S is benign, therefore the missing stabilizing contact between the two helices is probably not so important.<br />
<br />
====G241E====<br />
<figure id="G241E"><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_G202E_sticks.png| Comparison of wild type (wt) Glycine 241 (magenta) and mutant Glutamate (orange). The mutated residue has a long side chain.<br />
File:2V3E_B_G202_hb.png| Glycine 241 (PDB 202) lies in a turn in the proximity of a helix end and in front of an another helix. Its amino-group forms a hydrogen bond to a residue of the latter helix. <br />
File:2V3E_B_mutant_202E_hb.png| The mutant Glutamate 241 maintains the same hydrogen bond (formed by the main chain) with a residue in a neighboring helix. The residue lies on the surface and the bigger side chain points to the outside of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''G241E''' (PDB 202) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
As no changes in the hydrogen bonds or clashes etc. can be noticed, we would predict the mutation G241E as benign. However, it is a disease causing mutation. Maybe the effect is caused by the polarity and negative charge of the mutant Glutamate, instead of the nonpolar and neutral Glycine.<br />
<br />
====N409S====<br />
<figure id="N409S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N370S_sticks.png| Comparison of wild type (wt) Asparagine 409 (magenta) and mutant Serine (orange). The mutated side chain is a little bit smaller, but points towards the same direction.<br />
File:2V3E_B_N370_hb.png| Asparagine 409 (PDB 370) is located in a helix. It builds five hydrogen bonds: four with the main chain and one with the side chain. Two of the main chain contacts are formed by the carboxyl-group: one with a helix residue and one with a residue located in a proximate beta-strand beginning, the two binding partners are separated by a short turn. Two other main chain hydrogen bonds are built by the amino-group and two carboxyl-groups of an amino acid in the helix (from the other side). The fifth bond is formed between the side chain amino-group and one of the caroboxy-groups of the last mentioned helix residue.<br />
File:2V3E_B_mutant_370S_hb.png| Hydrogen bonds formed by the mutant Serine 409. The five hydrogen bonds described in the figure on the left with the Asparagine 409 contacts are conserved.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N409S''' (PDB 370) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
Due to the fact that all hydrogen bonds remain conserved in the mutant and that no clashes etc. could be detected, we would predict the mutation N409S as non-effect. Moreover, the both amino acids, Asparagine and Serine, are both polar and neutral. Nevertheless, this mutation is annotated as disease causing. <!-- Do I oversee something?--><br />
<br />
====L483P====<br />
<figure id="L483P" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_L444P_sticks.png| Comparison of wild type (wt) Leucine 483 (magenta) and mutant Proline (orange). The mutant Proline is a cycle amino acid, which has very different properties than the hydrophobic Leucine. Is leads to a shift in the main chain conformation "backwards", so that the beginning of the neighboring strand is shifted. <br />
File:2V3E_B_L444_hb.png| Leucine 483 (PDB 444) lies at the very beginning of a beta strand. There is another beta strand in the proximity on the right, which is followed by three others, forming a beta sheet. However, the Leucine does not form any contact with the neighboring strand, as its side chain is hydrophobic and points in another direction. The Leucine forms an interesting hydrogen bond with its amino-group to the side chain of a residue located in the neighboring turn. After this loop another beta-strand follows.<br />
File:2V3E_B_mutant_444P_hb_sticks_missing_contact.png| The mutant Proline 483 does not form a hydrogen bond with the residue in the loop, like the Leucine main chain amino-group does (see the corresponding figure). The reason for this is that Proline lacks a free amino-group due to the cycle formation.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''L483P''' (PDB 444) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
We would predict the mutation L483P as having an effect - and so it is according to annotations.<br />
<br />
==Energy comparisons==<br />
[[Gaucher Disease: Task 09 - Lab Journal|Lab journal]]<br />
<br />
===foldX===<br />
<br />
We applied foldX for the five mutations to predict new structures with energies. <br />
<br />
<figtable id="scwrl_foldx"><br />
{| class="colBasic2"<br />
! || colspan="3" | SCWRL || colspan="5" | FoldX<br />
|-<br />
! Mutant || Energy || Mutant-WT || Predicted effect || Energy Mutant || Energy WT || Mutant-WT || Predicted effect || Observed effect<br />
|-<br />
| WT || 386.356 || || || || || || || <br />
|-<br />
| S77R || 386.706 || 0.350 || FALSE || 20.90 || 23.25 || -2.35 || FALSE || FALSE<br />
|-<br />
| N141S || 390.966 || 4.610 || TRUE || 28.81 || 28.84 || -0.03 || FALSE || FALSE<br />
|-<br />
| G241E || 394.319 || 7.963 || TRUE || 32.30 || 29.31 || 2.99 || TRUE || TRUE<br />
|-<br />
| N409S || 391.73 || 5.374 || TRUE || 29.08 || 27.77 || 1.31 || TRUE || TRUE<br />
|-<br />
| L483P || 424.715 || 38.359 || TRUE || 38.40 || 35.55 || 2.85 || TRUE || TRUE<br />
|}<br />
<center><small>'''<caption>''' Energies of the WT structure and the five mutant structures calculated by SQWRL and foldX. FoldX minimizes the energy of the WT structure for each mutation. The energy difference between each mutant and the respective WT structure was calculated. If it is (significantly) positive, the mutation is regarded as effect causing. The observed effect of the mutation is given for comparison. </caption></small></center><br />
</figtable><br />
<br />
We decided to regard all differences between the mutant and WT energies higher than 1 as significant. SCWRL identifies the three disease causing mutations as such. However, it also predicts one not disease causing mutation (N141S) as effect causing, thought with the smallest positive difference (higher 1) of 4.61. Interestingly, the most prevalent Gaucher disease mutation according to OMIM, L483P (L444P in OMIM), has a highest energy change of 38.359. FoldX makes a correct prediction in all cases, the two benign mutations even have a small negative energy change. However, the disease causing mutations have all only a small energy increase (1.31 - 2.99). In foldX the mutation L483P does not have the highest energy increase, in the contrary to SCWRL.<br />
<br />
Next, we superimposed the SCWRL and foldX structures in Pymol and compared them in the following figures.<br />
<br />
<figure id="scwrl_vs_foldx" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutant_38R.png| Mutation S77R. There is almost no difference in the conformation. However, foldX predicts two additional hydrogen bonds between the Argenine side chain: one to a residues in a proximate loop and the second is a big Tyrosine side chain from the same beta strand.<br />
File:2V3E_B_mutant_102S.png| Mutation N141S. There is almost no difference in the conformation and the same hydrogen bonds are predicted.<br />
File:2V3E_B_mutant_202E.png| Mutation G241E. In foldX the conformation of the Glutamate side chain is a little bit different, but it has no meaning, as it lies on the outside of the protein. Also the binding partner side chain has a little bit different angle, so that two hydrogen bonds, instead of only one in SCWRL, are formed.<br />
File:2V3E_B_mutant_370S.png| Mutation N409S. SCWRL and foldX agree in the conformation of the mutated and the binding residues, including the hydrogen bonds formed between them, apart from tiny shifts.<br />
File:2V3E_B_mutant_444P.png| Mutation L483P. Also here SCWRL and foldX predict nearly the same conformation of the Proline (and the residue which forms a hydrogen bond in WT with the Leucine).<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL (gray protein, orange mutated residue, yellow polar contacts) and foldX (lime protein, cyan mutated residue, dark cyan polar contacts).</caption></small><br />
</figure><br />
<br />
===Minimise===<br />
<br />
We applied minimise for all mutant structures produced by SCWRL and foldX and the WT structure. We used the output of one minimisation for another run as input 5 times for each structure. The <xr id="minimise"/> summarizes the resulting energies:<br />
<br />
<br />
<figtable id="minimise"><br />
{| class="colBasic2"<br />
! Method || Mutation || Round 1 || Round 2 || Round 3 || Round 4 || Round 5<br />
|-<br />
! - <br />
|| WT || style="background-color: #FFD700"| -12360.0 || -11661.9 || -12079.7 || -11636.9 || -11630.6<br />
|-<br />
! rowspan="5" | SCWRL<br />
|| S77R || -3482.3 || style="background-color: #FFD700"| -12111.1 || -11724.2 || -11509.3 || -11260.2<br />
|-<br />
|| N141S || -3593.4 || style="background-color: #FFD700"| -12199.7 || -11787.7 || -11568.1 || -11328.3<br />
|-<br />
|| G241E || -3548.4 || style="background-color: #FFD700"| -12163.9 || -11792.4 || -11593.1 || -11381.2<br />
|-<br />
|| N409S || -3631.0 || style="background-color: #FFD700"| -12214.2 || -11801.9 || -11594.4 || -11347.0<br />
|-<br />
|| L483P || -3469.4 || style="background-color: #FFD700"| -11900.1 || -11610.9 || -11665.8 || -11330.6<br />
|-<br />
!rowspan="5" | foldX <br />
|| S77R || style="background-color: #FFD700"| -12297.0 || -11892.1 || -12037.2 || -11707.4 || -11703.1<br />
|-<br />
|| N141S || style="background-color: #FFD700"| -12359.7 || -11961.2 || -11983.4 || -11550.9 || -11515.9<br />
|-<br />
|| G241E || style="background-color: #FFD700"| -12356.6 || -11944.8 || -12103.6 || -11782.5 || -11764.0<br />
|-<br />
|| N409S || style="background-color: #FFD700"| -12389.9 || -11966.0 || -12117.7 || -11845.4 || -11808.9<br />
|-<br />
|| L483P || style="background-color: #FFD700"| -12335.4 || -11925.2 || -12096.9 || -11771.4 || -11771.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Energies produced by each of five consecutive runs of the program '''minimise''', applied on the WT structure and the five mutant structures calculated by SQWRL and foldX. The lowest energies for each structure are marked gold.</caption></small></center><br />
</figtable><br />
<br />
For the WT, the lowest energy is reached after the first iteration of minimise. This is because the structure is already correct and therefore optimal. Interestingly, the lowest energy of the SQWRL mutants is reached after two minimise iterations, whereas for the foldX mutants the minimal energy is reached already after the first iteration. This may be explained by the fact that foldX already performs a minimisation step. Moreover, foldX minimal energies are always lower, than those calculated by SCWRL.<br />
<br />
Another interesting observation is that after reaching the lowest energy in the second minimise iterations in the WT and the foldX mutants, the energy rises constantly in the consequent runs. This probably means that the minimal local energy is reached in the second iteration and then the computation of the gradient at the minimal point can only lead to a higher energy value, maybe to another local minimum with a higher energy. A different pattern happens during the minimisation of the SCWRL mutants: after reaching the local minimal energy already after the first iteration, in the second iteration the energy rises, however it falls a little again in the third iteration, thought not into the same local minimum as in the first iteration. This indicates that a second "suboptimal" local minimum is found. After the forth iteration the energy rises again. <br />
<br />
We compared the mutant structures in Pymol again after the minimise runs reaching the best energies: after the second round for SQWRL and the first round for foldX (<xr id="minimise"/>).<br />
<br />
<figure id="minimise" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_S38R_minimised-scwrl_vs_foldx.png| Mutation S77R.<br />
File:2V3E_B_N102S_minimised-scwrl_vs_foldx.png| Mutation N141S.<br />
File:2V3E_B_G202E_minimised-scwrl_vs_foldx.png| Mutation G241E.<br />
File:2V3E_B_N370S_minimised-scwrl_vs_foldx.png| Mutation N409S. <br />
File:2V3E_B_L444P_minimised-scwrl_vs_foldx.png| Mutation L483P.<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL after two minimise iterations (gray protein, orange mutated residue) and foldX after one minimise iteration (lime protein, cyan mutated residue). There is almost no difference in the conformations of all the mutations. </caption></small><br />
</figure><br />
<br />
To conclude, SQWRL and foldX are both pretty good tools for calculating the energy difference and the structural change of a protein after a point mutation. There is almost no difference in the resulting structures between the two programs, in particular the predicted conformations of the mutated residues and their environments are very similar.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_09_-_Structure-based_mutation_analysis&diff=38931
Gaucher Disease: Task 09 - Structure-based mutation analysis
2013-09-28T17:23:28Z
<p>Kalemanovm: /* foldX */</p>
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<br />
==Preparation==<br />
<br />
===1. Choice of a structure to work with===<br />
<br />
We chose the structure '''2V3E, chain B''', which has the following properties:<br />
<br />
<figtable id="2V3E"><br />
{|class="colBasic2"<br />
!PDB-ID || Resolution (Å) || Chain || Covered residues (UniProt seq.) || Missing residues (ATOM seq.) || Covered residues (ATOM seq.) || R-Value(obs.) || R-Free || pH || Temperature (K)<br />
|-<br />
|[http://www.pdb.org/pdb/files/2V3E.pdb 2V3E] || 2.0 || A/B || 40-536 (92.7%) || A: 31, (498-503), B: (-1), (498-503) || A: -1-30, 32-497, B: '''0-497''' || 0.163 || 0.220 || '''7.5''' || 100<br />
|}<br />
<center><small>'''<caption>''' Properties of 2V3E, chain B, the chosen reference structure of GBA sequence P04062.</caption></small></center><br />
</figtable><br />
<br />
For more information about other candidates and the missing residues, see the [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].<br />
<br />
===2. Visualization of the mutations to work with===<br />
<br />
We selected the following five mutation from the mutations selected in for this task:<br />
<br />
<figtable id="mutations"><br />
{|class="colBasic2"<br />
! Reference || Codon change || Codon Number (UniProt) || Codon Number (PDB) || Amino Acid change || Polarity || Charge (pH) || Disease causing?<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234]|| AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || 77 || 38 || Ser ⇒ Arg (S77R) ||polar ⇒ polar || neutral ⇒ positive || FALSE<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673]|| A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || 141 || 102 || Asn ⇒ Ser (N141S) || polar ⇒ polar || neutral ⇒ neutral || FALSE <br />
|-<br />
| CM992894|| G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A|| 241 || 202 || Gly ⇒ Glu (G241E) || nonpolar ⇒ polar || neutral ⇒ negative || TRUE<br />
|-<br />
| CM880036|| A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || 409 || 370 || Asn ⇒ Ser (N409S) || polar ⇒ polar || neutral ⇒ neutral || TRUE <br />
|- <br />
| CM870010|| C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || 483 || 444 || Leu ⇒ Pro (L483P) || nonpolar ⇒ nonpolar || neutral ⇒ neutral || TRUE<br />
|}<br />
<center><small>'''<caption>''' Selected mutations of GBA sequence P04062. Mapping of the UniProt positions onto the PDB ATOM sequence is given. </caption></small></center><br />
</figtable><br />
<br />
The following figure visualizes the five residues we are going to mutate on the reference structure, 2V3E, chain B.<br />
<br />
<figure id="pymol_mutations" ><br />
<gallery widths=550px heights=300px perrow="2"><br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-labeled.png|The five residues to be mutated mapped onto the structure 2V3E, chain B. Those residues are represented as green (not disease causing S77R and N141S) and red (disease causing G241E, N409S and L483P) spheres. The ligands bound to the structure at three binding sites during its resolution (BMA, FUC, NAG and NND) are represented as spheres in different shades of orange. Neither of the five residues lies in the proximity of one of the binding sites. <br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-other_side-labeled.png| Same as the first figure, only view from the other side of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of five residues of the structure 2V3E, chain B, we are going to mutate as described in Table 2.</caption></small><br />
</figure><br />
<br />
As can be seen on the image, none of the residues to be mutated lies in the proximity of one of the three binding sites. (See also the second subfigures of the figure galleries in the next subsection for close up on the residues and their hydrogen bonds.) However, four of the residues lie within a secondary structure element (beta sheet or helix) and one - Glycine 241 - in a turn<br />
near a helix. This implies that exchange of these residues with others with different functional groups, polarity and charge could lead to destruction of some hydrogen bonds within or between the secondary structures (e.g. Asparagine 141). This might lead to structural changes and even to destruction of the secondary structures or important blocks of secondary structure elements. Moreover, an exchange with a side chain of a bigger size might lead to clashed with proximate residues (e.g. with the loop near the Serine 77).<br />
<br />
===3. Creation of mutated structures===<br />
<br />
We used SQWRL4 to create the five mutated structures. (See [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].) The mutated residues in comparison to the native residues, the hydrogen of the mutants and possible clashes are shown in the following figures.<br />
<br />
====S77R====<br />
<figure id="S77R" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_S38R_sticks.png| Comparison of wild type (wt) Serine 77 (magenta) and mutant Argenine (orange). The mutated residue has a longer side chain.<br />
File:2V3E_B_S38_hb.png| Serine 77 (PDB 38) is colored magenta, its chain is in the stick representation and all the backbone residues are in the cartoon representation (the same for the following subfigures). Serine 77 lies in the middle of a beta strand flanked by two other strands in an anti-parallel order. It forms two hydrogen bonds with a residue in a neighboring strand.<br />
File:2V3E_B_mutant_38R_hb.png| Hydrogen bonds formed by the mutant Argenine 77 remain conserved, as they are formed by the main chain.<br />
File:2V3E_B_mutant_38R_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Argenine 77 (orange) shows, that its side chain does not clash with the neighboring loop (gray spheres).<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''S77R''' (PDB 38) of the structure 2V3E, chain B, with SCWRL.</caption></small><br />
</figure><br />
<br />
According to this images, we would also predict the mutation S77R as not disease causing.<br />
<br />
====N141S====<br />
<figure id="N141S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N102S_sticks.png| Comparison of wild type (wt) Asparagine 141 (magenta) and mutant Serine (orange). The mutated side chain is a little shorter and points towards another direction.<br />
File:2V3E_B_N102_hb.png| Asparagine 141 (PDB 102) is located in a helix and forms four hydrogen contacts: two with the main-chain carboxyl- and amino-groups with two helix amino acids (one in each direction) and two with the side chain. One of the contacts of the Asparagine side chain also binds to the same residue in the helix as the Asparagine amino-group binds. The second hydrogen bond of the Asparagine side chain is formed with the side chain of a residue in the neighboring helix. Maybe this bond plays a stabilizing role between the two helices. <br />
File:2V3E_B_mutant_102S_hb_2diff.png| Hydrogen bonds formed by the mutant Serine 141. A contact that was formed between the native Asparagine side chain and a side chain pointing from a neighboring helix (showed in sticks) is missing, as well as a second contact with a residue in the same helix. Instead, the mutated Serine side chain forms a contact with another residue in the same helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_missing_contact.png| Spheres representation of the mutant Serine and the missing contact with the neighboring helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Serine and a proximate loop (gray spheres) - there is no clash.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N141S''' (PDB 102) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
The mutation N141S is benign, therefore the missing stabilizing contact between the two helices is probably not so important.<br />
<br />
====G241E====<br />
<figure id="G241E"><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_G202E_sticks.png| Comparison of wild type (wt) Glycine 241 (magenta) and mutant Glutamate (orange). The mutated residue has a long side chain.<br />
File:2V3E_B_G202_hb.png| Glycine 241 (PDB 202) lies in a turn in the proximity of a helix end and in front of an another helix. Its amino-group forms a hydrogen bond to a residue of the latter helix. <br />
File:2V3E_B_mutant_202E_hb.png| The mutant Glutamate 241 maintains the same hydrogen bond (formed by the main chain) with a residue in a neighboring helix. The residue lies on the surface and the bigger side chain points to the outside of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''G241E''' (PDB 202) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
As no changes in the hydrogen bonds or clashes etc. can be noticed, we would predict the mutation G241E as benign. However, it is a disease causing mutation. Maybe the effect is caused by the polarity and negative charge of the mutant Glutamate, instead of the nonpolar and neutral Glycine.<br />
<br />
====N409S====<br />
<figure id="N409S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N370S_sticks.png| Comparison of wild type (wt) Asparagine 409 (magenta) and mutant Serine (orange). The mutated side chain is a little bit smaller, but points towards the same direction.<br />
File:2V3E_B_N370_hb.png| Asparagine 409 (PDB 370) is located in a helix. It builds five hydrogen bonds: four with the main chain and one with the side chain. Two of the main chain contacts are formed by the carboxyl-group: one with a helix residue and one with a residue located in a proximate beta-strand beginning, the two binding partners are separated by a short turn. Two other main chain hydrogen bonds are built by the amino-group and two carboxyl-groups of an amino acid in the helix (from the other side). The fifth bond is formed between the side chain amino-group and one of the caroboxy-groups of the last mentioned helix residue.<br />
File:2V3E_B_mutant_370S_hb.png| Hydrogen bonds formed by the mutant Serine 409. The five hydrogen bonds described in the figure on the left with the Asparagine 409 contacts are conserved.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N409S''' (PDB 370) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
Due to the fact that all hydrogen bonds remain conserved in the mutant and that no clashes etc. could be detected, we would predict the mutation N409S as non-effect. Moreover, the both amino acids, Asparagine and Serine, are both polar and neutral. Nevertheless, this mutation is annotated as disease causing. <!-- Do I oversee something?--><br />
<br />
====L483P====<br />
<figure id="L483P" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_L444P_sticks.png| Comparison of wild type (wt) Leucine 483 (magenta) and mutant Proline (orange). The mutant Proline is a cycle amino acid, which has very different properties than the hydrophobic Leucine. Is leads to a shift in the main chain conformation "backwards", so that the beginning of the neighboring strand is shifted. <br />
File:2V3E_B_L444_hb.png| Leucine 483 (PDB 444) lies at the very beginning of a beta strand. There is another beta strand in the proximity on the right, which is followed by three others, forming a beta sheet. However, the Leucine does not form any contact with the neighboring strand, as its side chain is hydrophobic and points in another direction. The Leucine forms an interesting hydrogen bond with its amino-group to the side chain of a residue located in the neighboring turn. After this loop another beta-strand follows.<br />
File:2V3E_B_mutant_444P_hb_sticks_missing_contact.png| The mutant Proline 483 does not form a hydrogen bond with the residue in the loop, like the Leucine main chain amino-group does (see the corresponding figure). The reason for this is that Proline lacks a free amino-group due to the cycle formation.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''L483P''' (PDB 444) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
We would predict the mutation L483P as having an effect - and so it is according to annotations.<br />
<br />
==Energy comparisons==<br />
[[Gaucher Disease: Task 09 - Lab Journal|Lab journal]]<br />
<br />
===foldX===<br />
<br />
We applied foldX for the five mutations to predict new structures with energies. <br />
<br />
<figtable id="scwrl_foldx"><br />
{| class="colBasic2"<br />
! || colspan="3" | SCWRL || colspan="5" | FoldX<br />
|-<br />
! Mutant || Energy || Mutant-WT || Predicted effect || Energy Mutant || Energy WT || Mutant-WT || Predicted effect || Observed effect<br />
|-<br />
| WT || 386.356 || || || || || || || <br />
|-<br />
| S77R || 386.706 || 0.350 || FALSE || 20.90 || 23.25 || -2.35 || FALSE || FALSE<br />
|-<br />
| N141S || 390.966 || 4.610 || TRUE || 28.81 || 28.84 || -0.03 || FALSE || FALSE<br />
|-<br />
| G241E || 394.319 || 7.963 || TRUE || 32.30 || 29.31 || 2.99 || TRUE || TRUE<br />
|-<br />
| N409S || 391.73 || 5.374 || TRUE || 29.08 || 27.77 || 1.31 || TRUE || TRUE<br />
|-<br />
| L483P || 424.715 || 38.359 || TRUE || 38.40 || 35.55 || 2.85 || TRUE || TRUE<br />
|}<br />
<center><small>'''<caption>''' Energies of the WT structure and the five mutant structures calculated by SQWRL and foldX. FoldX minimizes the energy of the WT structure for each mutation. The energy difference between each mutant and the respective WT structure was calculated. If it is (significantly) positive, the mutation is regarded as effect causing. The observed effect of the mutation is given for comparison. </caption></small></center><br />
</figtable><br />
<br />
We decided to regard all differences between the mutant and WT energies higher than 1 as significant. SCWRL identifies the three disease causing mutations as such. However, it also predicts one not disease causing mutation (N141S) as effect causing, thought with the smallest positive difference (higher 1) of 4.61. Interestingly, the most prevalent Gaucher disease mutation according to OMIM, L483P (L444P in OMIM), has a highest energy change of 38.359. FoldX makes a correct prediction in all cases, the two benign mutations even have a small negative energy change. However, the disease causing mutations have all only a small energy increase (1.31 - 2.99). In foldX the mutation L483P does not have the highest energy increase, in the contrary to SCWRL.<br />
<br />
Next, we superimposed the SCWRL and foldX structures in Pymol and compared them in the following figures.<br />
<br />
<figure id="scwrl_vs_foldx" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutant_38R.png| Mutation S77R. There is almost no difference in the conformation. However, foldX predicts two additional hydrogen bonds between the Argenine side chain: one to a residues in a proximate loop and the second is a big Tyrosine side chain from the same beta strand.<br />
File:2V3E_B_mutant_102S.png| Mutation N141S. There is almost no difference in the conformation and the same hydrogen bonds are predicted.<br />
File:2V3E_B_mutant_202E.png| Mutation G241E. In foldX the conformation of the Glutamate side chain is a little bit different, but it has no meaning, as it lies on the outside of the protein. Also the binding partner side chain has a little bit different angle, so that two hydrogen bonds, instead of only one in SCWRL, are formed.<br />
File:2V3E_B_mutant_370S.png| Mutation N409S. SCWRL and foldX agree in the conformation of the mutated and the binding residues, including the hydrogen bonds formed between them, apart from tiny shifts.<br />
File:2V3E_B_mutant_444P.png| Mutation L483P. Also here SCWRL and foldX predict nearly the same conformation of the Proline (and the residue which forms a hydrogen bond in WT with the Leucine).<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL (gray protein, orange mutated residue, yellow polar contacts) and foldX (lime protein, cyan mutated residue, dark cyan polar contacts).</caption></small><br />
</figure><br />
<br />
===Minimise===<br />
<br />
We applied minimise for all mutant structures produced by SCWRL and foldX and the WT structure. We used the output of one minimisation for another run as input 5 times for each structure. The <xr id="minimise"/> summarizes the resulting energies:<br />
<br />
<br />
<figtable id="minimise"><br />
{| class="colBasic2"<br />
! Method || Mutation || Round 1 || Round 2 || Round 3 || Round 4 || Round 5<br />
|-<br />
! - <br />
|| WT || style="background-color: #FFD700"| -12360.0 || -11661.9 || -12079.7 || -11636.9 || -11630.6<br />
|-<br />
! rowspan="5" | SCWRL<br />
|| S77R || -3482.3 || style="background-color: #FFD700"| -12111.1 || -11724.2 || -11509.3 || -11260.2<br />
|-<br />
|| N141S || -3593.4 || style="background-color: #FFD700"| -12199.7 || -11787.7 || -11568.1 || -11328.3<br />
|-<br />
|| G241E || -3548.4 || style="background-color: #FFD700"| -12163.9 || -11792.4 || -11593.1 || -11381.2<br />
|-<br />
|| N409S || -3631.0 || style="background-color: #FFD700"| -12214.2 || -11801.9 || -11594.4 || -11347.0<br />
|-<br />
|| L483P || -3469.4 || style="background-color: #FFD700"| -11900.1 || -11610.9 || -11665.8 || -11330.6<br />
|-<br />
!rowspan="5" | foldX <br />
|| S77R || style="background-color: #FFD700"| -12297.0 || -11892.1 || -12037.2 || -11707.4 || -11703.1<br />
|-<br />
|| N141S || style="background-color: #FFD700"| -12359.7 || -11961.2 || -11983.4 || -11550.9 || -11515.9<br />
|-<br />
|| G241E || style="background-color: #FFD700"| -12356.6 || -11944.8 || -12103.6 || -11782.5 || -11764.0<br />
|-<br />
|| N409S || style="background-color: #FFD700"| -12389.9 || -11966.0 || -12117.7 || -11845.4 || -11808.9<br />
|-<br />
|| L483P || style="background-color: #FFD700"| -12335.4 || -11925.2 || -12096.9 || -11771.4 || -11771.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Energies produced by each of five consecutive runs of the program '''minimise''', applied on the WT structure and the five mutant structures calculated by SQWRL and foldX. The lowest energies for each structure are marked gold.</caption></small></center><br />
</figtable><br />
<br />
For the WT, the lowest energy is reached after the first iteration of minimise. This is because the structure is already correct and therefore optimal. Interestingly, the lowest energy of the SQWRL mutants is reached after two minimise iterations, whereas for the foldX mutants the minimal energy is reached already after the first iteration. This may be explained by the fact that foldX already performs a minimisation step. Moreover, foldX minimal energies are always lower, than those calculated by SCWRL.<br />
<br />
Another interesting observation is that after reaching the lowest energy in the second minimise iterations in the WT and the foldX mutants, the energy rises constantly in the consequent runs. This probably means that the minimal local energy is reached in the second iteration and then the computation of the gradient at the minimal point can only lead to a higher energy value, maybe to another local minimum with a higher energy. A different pattern happens during the minimisation of the SCWRL mutants: after reaching the local minimal energy already after the first iteration, in the second iteration the energy rises, however it falls a little again in the third iteration, thought not into the same local minimum as in the first iteration. This indicates that a second "suboptimal" local minimum is found. After the forth iteration the energy rises again. <br />
<br />
We compared the mutant structures in Pymol again after the minimise runs reaching the best energies: after the second round for SQWRL and the first round for foldX (<xr id="minimise"/>).<br />
<br />
<figure id="minimise" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_S38R_minimised-scwrl_vs_foldx.png| Mutation S77R.<br />
File:2V3E_B_N102S_minimised-scwrl_vs_foldx.png| Mutation N141S.<br />
File:2V3E_B_G202E_minimised-scwrl_vs_foldx.png| Mutation G241E.<br />
File:2V3E_B_N370S_minimised-scwrl_vs_foldx.png| Mutation N409S. <br />
File:2V3E_B_L444P_minimised-scwrl_vs_foldx.png| Mutation L483P.<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL after two minimise iterations (gray protein, orange mutated residue) and foldX after one minimise iteration (lime protein, cyan mutated residue). There is almost no difference in the conformations of all the mutations. </caption></small><br />
</figure><br />
<br />
To conclude, SQWRL and foldX are both pretty good tools for calculating the energy difference and the structural change of a protein after a point mutation. There is almost no difference in the resulting structures between the two programs, in particular the predicted conformations of the mutated residues and their environments are very similar.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_09_-_Structure-based_mutation_analysis&diff=38929
Gaucher Disease: Task 09 - Structure-based mutation analysis
2013-09-27T19:03:56Z
<p>Kalemanovm: /* 1. Choice of a structure to work with */</p>
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<br />
==Preparation==<br />
<br />
===1. Choice of a structure to work with===<br />
<br />
We chose the structure '''2V3E, chain B''', which has the following properties:<br />
<br />
<figtable id="2V3E"><br />
{|class="colBasic2"<br />
!PDB-ID || Resolution (Å) || Chain || Covered residues (UniProt seq.) || Missing residues (ATOM seq.) || Covered residues (ATOM seq.) || R-Value(obs.) || R-Free || pH || Temperature (K)<br />
|-<br />
|[http://www.pdb.org/pdb/files/2V3E.pdb 2V3E] || 2.0 || A/B || 40-536 (92.7%) || A: 31, (498-503), B: (-1), (498-503) || A: -1-30, 32-497, B: '''0-497''' || 0.163 || 0.220 || '''7.5''' || 100<br />
|}<br />
<center><small>'''<caption>''' Properties of 2V3E, chain B, the chosen reference structure of GBA sequence P04062.</caption></small></center><br />
</figtable><br />
<br />
For more information about other candidates and the missing residues, see the [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].<br />
<br />
===2. Visualization of the mutations to work with===<br />
<br />
We selected the following five mutation from the mutations selected in for this task:<br />
<br />
<figtable id="mutations"><br />
{|class="colBasic2"<br />
! Reference || Codon change || Codon Number (UniProt) || Codon Number (PDB) || Amino Acid change || Polarity || Charge (pH) || Disease causing?<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234]|| AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || 77 || 38 || Ser ⇒ Arg (S77R) ||polar ⇒ polar || neutral ⇒ positive || FALSE<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673]|| A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || 141 || 102 || Asn ⇒ Ser (N141S) || polar ⇒ polar || neutral ⇒ neutral || FALSE <br />
|-<br />
| CM992894|| G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A|| 241 || 202 || Gly ⇒ Glu (G241E) || nonpolar ⇒ polar || neutral ⇒ negative || TRUE<br />
|-<br />
| CM880036|| A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || 409 || 370 || Asn ⇒ Ser (N409S) || polar ⇒ polar || neutral ⇒ neutral || TRUE <br />
|- <br />
| CM870010|| C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || 483 || 444 || Leu ⇒ Pro (L483P) || nonpolar ⇒ nonpolar || neutral ⇒ neutral || TRUE<br />
|}<br />
<center><small>'''<caption>''' Selected mutations of GBA sequence P04062. Mapping of the UniProt positions onto the PDB ATOM sequence is given. </caption></small></center><br />
</figtable><br />
<br />
The following figure visualizes the five residues we are going to mutate on the reference structure, 2V3E, chain B.<br />
<br />
<figure id="pymol_mutations" ><br />
<gallery widths=550px heights=300px perrow="2"><br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-labeled.png|The five residues to be mutated mapped onto the structure 2V3E, chain B. Those residues are represented as green (not disease causing S77R and N141S) and red (disease causing G241E, N409S and L483P) spheres. The ligands bound to the structure at three binding sites during its resolution (BMA, FUC, NAG and NND) are represented as spheres in different shades of orange. Neither of the five residues lies in the proximity of one of the binding sites. <br />
File:2V3E_B_mutations_ligands_effect_colored_mesh_spheres-other_side-labeled.png| Same as the first figure, only view from the other side of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of five residues of the structure 2V3E, chain B, we are going to mutate as described in Table 2.</caption></small><br />
</figure><br />
<br />
As can be seen on the image, none of the residues to be mutated lies in the proximity of one of the three binding sites. (See also the second subfigures of the figure galleries in the next subsection for close up on the residues and their hydrogen bonds.) However, four of the residues lie within a secondary structure element (beta sheet or helix) and one - Glycine 241 - in a turn<br />
near a helix. This implies that exchange of these residues with others with different functional groups, polarity and charge could lead to destruction of some hydrogen bonds within or between the secondary structures (e.g. Asparagine 141). This might lead to structural changes and even to destruction of the secondary structures or important blocks of secondary structure elements. Moreover, an exchange with a side chain of a bigger size might lead to clashed with proximate residues (e.g. with the loop near the Serine 77).<br />
<br />
===3. Creation of mutated structures===<br />
<br />
We used SQWRL4 to create the five mutated structures. (See [[Gaucher Disease: Task 09 - Lab Journal|lab journal]].) The mutated residues in comparison to the native residues, the hydrogen of the mutants and possible clashes are shown in the following figures.<br />
<br />
====S77R====<br />
<figure id="S77R" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_S38R_sticks.png| Comparison of wild type (wt) Serine 77 (magenta) and mutant Argenine (orange). The mutated residue has a longer side chain.<br />
File:2V3E_B_S38_hb.png| Serine 77 (PDB 38) is colored magenta, its chain is in the stick representation and all the backbone residues are in the cartoon representation (the same for the following subfigures). Serine 77 lies in the middle of a beta strand flanked by two other strands in an anti-parallel order. It forms two hydrogen bonds with a residue in a neighboring strand.<br />
File:2V3E_B_mutant_38R_hb.png| Hydrogen bonds formed by the mutant Argenine 77 remain conserved, as they are formed by the main chain.<br />
File:2V3E_B_mutant_38R_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Argenine 77 (orange) shows, that its side chain does not clash with the neighboring loop (gray spheres).<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''S77R''' (PDB 38) of the structure 2V3E, chain B, with SCWRL.</caption></small><br />
</figure><br />
<br />
According to this images, we would also predict the mutation S77R as not disease causing.<br />
<br />
====N141S====<br />
<figure id="N141S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N102S_sticks.png| Comparison of wild type (wt) Asparagine 141 (magenta) and mutant Serine (orange). The mutated side chain is a little shorter and points towards another direction.<br />
File:2V3E_B_N102_hb.png| Asparagine 141 (PDB 102) is located in a helix and forms four hydrogen contacts: two with the main-chain carboxyl- and amino-groups with two helix amino acids (one in each direction) and two with the side chain. One of the contacts of the Asparagine side chain also binds to the same residue in the helix as the Asparagine amino-group binds. The second hydrogen bond of the Asparagine side chain is formed with the side chain of a residue in the neighboring helix. Maybe this bond plays a stabilizing role between the two helices. <br />
File:2V3E_B_mutant_102S_hb_2diff.png| Hydrogen bonds formed by the mutant Serine 141. A contact that was formed between the native Asparagine side chain and a side chain pointing from a neighboring helix (showed in sticks) is missing, as well as a second contact with a residue in the same helix. Instead, the mutated Serine side chain forms a contact with another residue in the same helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_missing_contact.png| Spheres representation of the mutant Serine and the missing contact with the neighboring helix.<br />
File:2V3E_B_mutant_102S_hb_spheres_no_loop_clash.png| Spheres representation of the mutant Serine and a proximate loop (gray spheres) - there is no clash.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N141S''' (PDB 102) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
The mutation N141S is benign, therefore the missing stabilizing contact between the two helices is probably not so important.<br />
<br />
====G241E====<br />
<figure id="G241E"><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_G202E_sticks.png| Comparison of wild type (wt) Glycine 241 (magenta) and mutant Glutamate (orange). The mutated residue has a long side chain.<br />
File:2V3E_B_G202_hb.png| Glycine 241 (PDB 202) lies in a turn in the proximity of a helix end and in front of an another helix. Its amino-group forms a hydrogen bond to a residue of the latter helix. <br />
File:2V3E_B_mutant_202E_hb.png| The mutant Glutamate 241 maintains the same hydrogen bond (formed by the main chain) with a residue in a neighboring helix. The residue lies on the surface and the bigger side chain points to the outside of the protein.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''G241E''' (PDB 202) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
As no changes in the hydrogen bonds or clashes etc. can be noticed, we would predict the mutation G241E as benign. However, it is a disease causing mutation. Maybe the effect is caused by the polarity and negative charge of the mutant Glutamate, instead of the nonpolar and neutral Glycine.<br />
<br />
====N409S====<br />
<figure id="N409S" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_N370S_sticks.png| Comparison of wild type (wt) Asparagine 409 (magenta) and mutant Serine (orange). The mutated side chain is a little bit smaller, but points towards the same direction.<br />
File:2V3E_B_N370_hb.png| Asparagine 409 (PDB 370) is located in a helix. It builds five hydrogen bonds: four with the main chain and one with the side chain. Two of the main chain contacts are formed by the carboxyl-group: one with a helix residue and one with a residue located in a proximate beta-strand beginning, the two binding partners are separated by a short turn. Two other main chain hydrogen bonds are built by the amino-group and two carboxyl-groups of an amino acid in the helix (from the other side). The fifth bond is formed between the side chain amino-group and one of the caroboxy-groups of the last mentioned helix residue.<br />
File:2V3E_B_mutant_370S_hb.png| Hydrogen bonds formed by the mutant Serine 409. The five hydrogen bonds described in the figure on the left with the Asparagine 409 contacts are conserved.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''N409S''' (PDB 370) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
Due to the fact that all hydrogen bonds remain conserved in the mutant and that no clashes etc. could be detected, we would predict the mutation N409S as non-effect. Moreover, the both amino acids, Asparagine and Serine, are both polar and neutral. Nevertheless, this mutation is annotated as disease causing. <!-- Do I oversee something?--><br />
<br />
====L483P====<br />
<figure id="L483P" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutation_L444P_sticks.png| Comparison of wild type (wt) Leucine 483 (magenta) and mutant Proline (orange). The mutant Proline is a cycle amino acid, which has very different properties than the hydrophobic Leucine. Is leads to a shift in the main chain conformation "backwards", so that the beginning of the neighboring strand is shifted. <br />
File:2V3E_B_L444_hb.png| Leucine 483 (PDB 444) lies at the very beginning of a beta strand. There is another beta strand in the proximity on the right, which is followed by three others, forming a beta sheet. However, the Leucine does not form any contact with the neighboring strand, as its side chain is hydrophobic and points in another direction. The Leucine forms an interesting hydrogen bond with its amino-group to the side chain of a residue located in the neighboring turn. After this loop another beta-strand follows.<br />
File:2V3E_B_mutant_444P_hb_sticks_missing_contact.png| The mutant Proline 483 does not form a hydrogen bond with the residue in the loop, like the Leucine main chain amino-group does (see the corresponding figure). The reason for this is that Proline lacks a free amino-group due to the cycle formation.<br />
</gallery><br />
<small>'''<caption>''' PyMol visualization of the mutation '''L483P''' (PDB 444) of the structure 2V3E, chain B, created with SCWRL.</caption></small><br />
</figure><br />
<br />
We would predict the mutation L483P as having an effect - and so it is according to annotations.<br />
<br />
==Energy comparisons==<br />
[[Gaucher Disease: Task 09 - Lab Journal|Lab journal]]<br />
<br />
===foldX===<br />
<br />
We applied foldX for the five mutations to predict new structures with energies. <br />
<br />
<figtable id="scwrl_foldx"><br />
{| class="colBasic2"<br />
! || colspan="3" | SCWRL || colspan="5" | FoldX<br />
|-<br />
! Mutant || Energy || Mutant-WT || Predicted effect || Energy Mutant || Energy WT || Mutant-WT || Predicted effect || Observed effect<br />
|-<br />
| WT || 386.356 || || || || || || || <br />
|-<br />
| S77R || 386.706 || 0.350 || FALSE || 20.90 || 23.25 || -2.35 || FALSE || FALSE<br />
|-<br />
| N141S || 390.966 || 4.610 || TRUE || 28.81 || 28.84 || -0.03 || FALSE || FALSE<br />
|-<br />
| G241E || 394.319 || 7.963 || TRUE || 32.30 || 29.31 || 2.99 || TRUE || TRUE<br />
|-<br />
| N409S || 391.73 || 5.374 || TRUE || 29.08 || 27.77 || 1.31 || TRUE || TRUE<br />
|-<br />
| L483P || 424.715 || 38.359 || TRUE || 38.40 || 35.55 || 2.85 || TRUE || TRUE<br />
|}<br />
<center><small>'''<caption>''' Energies of the WT structure and the five mutant structures calculated by SQWRL and foldX. FoldX minimizes the energy of the WT structure for each mutation. The energy difference between each mutant and the respective WT structure was calculated. If it is (significantly) positive, the mutation is regarded as effect causing. The observed effect of the mutation is given for comparison. </caption></small></center><br />
</figtable><br />
<br />
We decided to regard all differences between the mutant and WT energies higher than 1 as significant. SCWRL identifies the three disease causing mutations as such. However, it also predicts one not disease causing mutation (N141S) as effect causing, thought with the smallest positive difference (higher 1) of 4.61. Interestingly, the most prevalent Gaucher disease mutation according to OMIM, L483P (L444P in OMIM), has a highest energy change of 38.359. FoldX makes a correct prediction in all cases, the two benign mutations even have a small negative energy change. However, the disease causing mutations have all only a small energy increase (1.31 - 2.99). In foldX the mutation L483P does not have the highest energy increase, in the contrary to SCWRL.<br />
<br />
Next, we superimposed the SCWRL and foldX structures in Pymol and compared them in the following figures.<br />
<br />
<figure id="scwrl_vs_foldx" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_mutant_38R.png| Mutation S77R. There is almost no difference in the conformation. However, foldX predicts two additional hydrogen bonds between the Argenine side chain: one to a residues in a proximate loop and the second is a big Tyrosine side chain from the same beta strand.<br />
File:2V3E_B_mutant_102S.png| Mutation N141S. There is almost no difference in the conformation and the same hydrogen bonds are predicted.<br />
File:2V3E_B_mutant_202E.png| Mutation G241E. In foldX the conformation of the Glutamate side chain is a little bit different, but it has no meaning, as it lies on the outside of the protein. Also the binding partner side chain has a little bit different angle, so that two hydrogen bonds, instead of of only one in SCWRL, are formed.<br />
File:2V3E_B_mutant_370S.png| Mutation N409S. SCWRL and foldX agree in the conformation of the mutated and the binding residues, including the hydrogen bonds formed between them, apart from tiny shifts.<br />
File:2V3E_B_mutant_444P.png| Mutation L483P. Also here SCWRL and foldX predict nearly the same conformation of the Proline (and the residue which forms a hydrogen bond in WT with the Leucine).<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL (gray protein, orange mutated residue, yellow polar contacts) and foldX (lime protein, cyan mutated residue, dark cyan polar contacts).</caption></small><br />
</figure><br />
<br />
===Minimise===<br />
<br />
We applied minimise for all mutant structures produced by SCWRL and foldX and the WT structure. We used the output of one minimisation for another run as input 5 times for each structure. The <xr id="minimise"/> summarizes the resulting energies:<br />
<br />
<br />
<figtable id="minimise"><br />
{| class="colBasic2"<br />
! Method || Mutation || Round 1 || Round 2 || Round 3 || Round 4 || Round 5<br />
|-<br />
! - <br />
|| WT || style="background-color: #FFD700"| -12360.0 || -11661.9 || -12079.7 || -11636.9 || -11630.6<br />
|-<br />
! rowspan="5" | SCWRL<br />
|| S77R || -3482.3 || style="background-color: #FFD700"| -12111.1 || -11724.2 || -11509.3 || -11260.2<br />
|-<br />
|| N141S || -3593.4 || style="background-color: #FFD700"| -12199.7 || -11787.7 || -11568.1 || -11328.3<br />
|-<br />
|| G241E || -3548.4 || style="background-color: #FFD700"| -12163.9 || -11792.4 || -11593.1 || -11381.2<br />
|-<br />
|| N409S || -3631.0 || style="background-color: #FFD700"| -12214.2 || -11801.9 || -11594.4 || -11347.0<br />
|-<br />
|| L483P || -3469.4 || style="background-color: #FFD700"| -11900.1 || -11610.9 || -11665.8 || -11330.6<br />
|-<br />
!rowspan="5" | foldX <br />
|| S77R || style="background-color: #FFD700"| -12297.0 || -11892.1 || -12037.2 || -11707.4 || -11703.1<br />
|-<br />
|| N141S || style="background-color: #FFD700"| -12359.7 || -11961.2 || -11983.4 || -11550.9 || -11515.9<br />
|-<br />
|| G241E || style="background-color: #FFD700"| -12356.6 || -11944.8 || -12103.6 || -11782.5 || -11764.0<br />
|-<br />
|| N409S || style="background-color: #FFD700"| -12389.9 || -11966.0 || -12117.7 || -11845.4 || -11808.9<br />
|-<br />
|| L483P || style="background-color: #FFD700"| -12335.4 || -11925.2 || -12096.9 || -11771.4 || -11771.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Energies produced by each of five consecutive runs of the program '''minimise''', applied on the WT structure and the five mutant structures calculated by SQWRL and foldX. The lowest energies for each structure are marked gold.</caption></small></center><br />
</figtable><br />
<br />
For the WT, the lowest energy is reached after the first iteration of minimise. This is because the structure is already correct and therefore optimal. Interestingly, the lowest energy of the SQWRL mutants is reached after two minimise iterations, whereas for the foldX mutants the minimal energy is reached already after the first iteration. This may be explained by the fact that foldX already performs a minimisation step. Moreover, foldX minimal energies are always lower, than those calculated by SCWRL.<br />
<br />
Another interesting observation is that after reaching the lowest energy in the second minimise iterations in the WT and the foldX mutants, the energy rises constantly in the consequent runs. This probably means that the minimal local energy is reached in the second iteration and then the computation of the gradient at the minimal point can only lead to a higher energy value, maybe to another local minimum with a higher energy. A different pattern happens during the minimisation of the SCWRL mutants: after reaching the local minimal energy already after the first iteration, in the second iteration the energy rises, however it falls a little again in the third iteration, thought not into the same local minimum as in the first iteration. This indicates that a second "suboptimal" local minimum is found. After the forth iteration the energy rises again. <br />
<br />
We compared the mutant structures in Pymol again after the minimise runs reaching the best energies: after the second round for SQWRL and the first round for foldX (<xr id="minimise"/>).<br />
<br />
<figure id="minimise" ><br />
<gallery widths=300px heights=200px perrow="3"><br />
File:2V3E_B_S38R_minimised-scwrl_vs_foldx.png| Mutation S77R.<br />
File:2V3E_B_N102S_minimised-scwrl_vs_foldx.png| Mutation N141S.<br />
File:2V3E_B_G202E_minimised-scwrl_vs_foldx.png| Mutation G241E.<br />
File:2V3E_B_N370S_minimised-scwrl_vs_foldx.png| Mutation N409S. <br />
File:2V3E_B_L444P_minimised-scwrl_vs_foldx.png| Mutation L483P.<br />
</gallery><br />
<small>'''<caption>''' Comparison between the mutants created with SCWRL after two minimise iterations (gray protein, orange mutated residue) and foldX after one minimise iteration (lime protein, cyan mutated residue). There is almost no difference in the conformations of all the mutations. </caption></small><br />
</figure><br />
<br />
To conclude, SQWRL and foldX are both pretty good tools for calculating the energy difference and the structural change of a protein after a point mutation. There is almost no difference in the resulting structures between the two programs, in particular the predicted conformations of the mutated residues and their environments are very similar.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_07_-_Research_SNPs&diff=38910
Gaucher Disease: Task 07 - Research SNPs
2013-09-18T14:33:00Z
<p>Kalemanovm: /* SNP Databases Summary */</p>
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<br />
==HGMD==<br />
[http://www.biobase-international.com/product/hgmd HGMD professional] was last updated in March 2013. The free public [http://www.hgmd.org/ HGMD] version provides data from the release of HGMD professional in 2008.<br />
The database contains the first example of disease causing or disease associated mutations as well as disease-associated/functional polymorphisms. The information is taken from literature or reported functional studies.<br />
Only non-silent mutations in the coding region are considered. Each mutation is stored once in the database. Additional to the information shown in the second table below, the information about a mutation includes its associated disease phenotype, chromosomal location, gene symbol and the reference of its first literature report. HGMD professional also provides a mutation viewer.<br />
The database has access to more than 250 Journals, that are searched by a combination of computerized and manual procedures to find new detected mutations in published articles about germline mutations causing human genetic diseases.<br />
<br />
<br />
The glucocerebrosidase has the gene symbol ''GBA'' and the accession number NM_001005741.2 . A research on The Human Gene Mutation Database for ''GBA'' leads to the results in <xr id="hgmd"/>.<br />
<br />
<figtable id="hgmd"><br />
{|class="colBasic2" <br />
! colspan="3" | ''GBA'' in HGMD<br />
|-<br />
! Mutation Type || Number of Mutations || Effect of Mutation<br />
|-<br />
|'''Missense/nonsense''' || '''256''' || substitutions of a single base-pair in coding regions, that cause a amino acid or stop/start codon change<br />
|-<br />
|Splicing || 16 || mutations that influence the mRNA splicing <br />
|-<br />
|Regulatory|| 0 || different Regulation caused by substitution<br />
|-<br />
|Small deletions || 26 || micro-deletions (< 21 bp) <br />
|-<br />
| Small insertions || 13 || micro-insertions (<21 bp)<br />
|-<br />
|Small indels || 4 || micro-indels (<21 bp)<br />
|-<br />
|Gross deletions || 3 || deletion (>20 bp)<br />
|-<br />
|Gross insertions/duplications || 1 || insertion (>20 bp)<br />
|-<br />
|Complex rearrangements || 16 || rearrangement of DNA fragments within the sequence<br />
|-<br />
|Repeat variations || 0 || different number of repeats<br />
|-<br />
|'''Total''' || '''335''' ||<br />
|}<br />
<center><small>'''<caption>''' Mutations found for gene symbol GBA on the database HGMD. </caption></small></center><br />
</figtable><br />
<br />
''GBA'' is not affected by a regulatory mutations and repeated variations. The mutations on ''GBA'' do not only result to Gaucher's disease or influence its phenotype, but may also have an effect on the phenotype of the diseases Parkinson and Alzheimer. <br />
HGMD public (2008 update) provides 335 mutations for ''GBA'' (table 1). On HGMD Professional 2013.1, 380 mutations can be found.<br />
<br />
<br />
<xr id="miss"/> of missense/nonsense mutations on ''GBA'' shows an abridgment of 10 mutations out of all 256 known missense/nonsense mutation (<xr id="hgmd"/>)of the public version of HGMD. The high number of mutations shows the high liability of ''GBA'' on Gaucher causing mutations. Some position specific amino acids have a higher liability to causing a the Gaucher disease, as more than one missense/nonsense mutation occur on that position (for example on sequence position 54 in <xr id="miss"/>). <br />
<br />
<br />
<figtable id="miss"><br />
{|class="colBasic2"<br />
! colspan="4" | Missense/nonsense mutations on ''GBA''<br />
|-<br />
! Accession Number || Codon change || Amino acid change || Codon Number<br />
|-<br />
|CM081634||c'''G'''GC-'''A'''GC||Gly-Ser||49<br />
|-<br />
|CM057078||A'''G'''C-A'''T'''C||Ser-Ile||51<br />
|-<br />
|CM044630||g'''G'''TG-'''A'''TG||Val-Met||54<br />
|-<br />
|CM960691||g'''G'''TG-'''C'''TG||Val-Leu||54<br />
|-<br />
|HM971738||T'''G'''T-T'''C'''T||Cys-Ser||55<br />
|-<br />
|CM081630||A'''G'''T-A'''A'''T||Ser-Asn||81<br />
|-<br />
|CM950560||A'''C'''A-A'''T'''A||Thr-Ile||82<br />
|-<br />
|CM960692||G'''G'''G-G'''A'''G||Gly-Glu||85<br />
|-<br />
|CM016030||g'''C'''GA-'''T'''GA||Arg-Term||86<br />
|-<br />
|CM950561||a'''C'''GG-'''T'''GG||Arg-Trp||87<br />
|}<br />
<center><small>'''<caption>''' First 10 missense/non-sense mutation (out of 256) listed on HGMD for GBA. </caption></small></center><br />
</figtable><br />
<br />
==dbSNP==<br />
The available data in dbSNP (version 138) was released on April 25, 2013. It provides mutation information for the three organisms, ''Homo sapiens'', ''Mus musculus'' and ''Bos taurus''. The database contains single base nucleotide substitutions as well as short deletion and insertion polymorphisms. The database is provided by NCBI, which gets the research information from the National Human Genome Research Institute.<br />
<br />
A point mutation can be classified as synonymous or non-synonymous, caused by insertions and deletions. A synonymous SNP has no effect on the resulting protein, as the mutating nucleotide still results into the same amino acid. A non-synonymous point mutation causes to a different amino acid. However, also a non-synonymous SNP may have no effect on the protein structure. In case a snp appears on a position which is not important for the protein structure or the mutated amino acids has a similar characteristic than its real amino acid, the structure of the protein is not affected and the protein function is still preserved. But in worst case, the point mutation generates a stop codon so that the protein is incomplete and thus not functional. Also SNPs on so called hot spots can destroy the protein structure and result to an inoperative protein. <br />
<br />
In the database 150 mutation were found for ''Homo sapiens'' on the gene GBA. 61 of them are silent mutations. Some chosen silent mutations are listed in <xr id="dbsnp"/>.<br />
<br />
<figtable id="dbsnp"><br />
{|class="colBasic2" <br />
! colspan="5" | Silent mutations on GBA<br />
|-<br />
! colspan="2" | mRNA || colspan="2" | Protein || rowspan="2" | refNumber<br />
|-<br />
! Allele change||Sequence position||Residue||Codon position<br />
|-<br />
|TC'''G''' ⇒ TC'''A'''||530||Ser|| 35 || [http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=148001886 rs148001886]<br />
|-<br />
|CC'''C''' ⇒ CC'''T'''||908|| Pro || 161|| [http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=79767521 rs79767521]<br />
|-<br />
|CT'''G''' ⇒ CT'''T'''|| 1262|| Leu || 279 || [http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?rs=371576958 rs371576958]<br />
|}<br />
<center><small>'''<caption>''' Randomly chosen, silent mutations of ''GBA'' with accession numbers NM_001005741.2 (gene) and NP_001005741.1 (protein) on dbSNP. </caption></small></center><br />
</figtable><br />
<br />
"SNP Geneview Report" of the "gene" search of the ''GBA human'' gene which lists all mutations: [[http://www.ncbi.nlm.nih.gov/SNP/snp_ref.cgi?locusId=2629]].<br />
<br />
<br />
<!--In dbSNP, look for silent (point) mutations. In most cases, significantly more than 100 mutations are known. The residue numbers in the databases may not correspond to each other.--><br />
<br />
==SNPedia==<br />
"[http://www.snpedia.com/index.php/GBA SNPedia] selects everything that is worth to be stored." [http://www.snpedia.com/index.php/SNPedia:FAQ] SNPs with characteristics to significant medical or genealogical consequences or that are common, reproducible and/or have other historic or medical significance. <br />
gives a short summary of known information of a gene taken from literature and internet. As SNPedia is a wiki, this information is most probably taken from Wikipedia. It refers to well known databases like OMIM or dbSNP as well as to some others like Google, 23andMe, FamilyTreeDNA or National Geographic [http://www.snpedia.com/index.php/SNPedia:FAQ]. Data from this side should be taken carefully, as not all content of SNPedia make a serious impression. It also links to a web service called [http://www.snpedia.com/index.php/Promethease Promethease] which allows the user to check their own DNA against the database. <br />
Its last update took place on 16th May 2013. In the current version SNPedia contents information of SNPs about 78 genomes.<br />
<br />
A search of GBA on SNPedia founds 48 SNPs on other webservices.<br />
<br />
==SNPdbe==<br />
<br />
*The following information (if available) is given on each SAAS (single amino acid substitution):<br />
**Experimentally derived functional and structural impact<br />
**Predicted functional effect<br />
**Associated disease<br />
**Average heterozygosity<br />
**Experimental evidence of the nsSNP<br />
**Evolutionary conservation of wildtype and mutant amino acid<br />
**Link-outs to external databases<br />
<br />
*Last update: 2012-02-20 (updated to recent Swiss-Prot release (2012-01))<br />
<br />
*The information comes from various databases (SwissProt, PMD, dbSNP, 1000 Genomes) and SNP effect is predicted with SIFT and SNAP<br />
<br />
*Currently (2013-06-23) 159142 protein sequences from 2985 organisms are covered in SNPdbe and 1691464 SAASs are referenced, consisting of natural variants, SAASs from mutagenesis experiments and sequencing conflicts<br />
<br />
<figtable id="snpdbe_human"><br />
{|class="colBasic2" <br />
! colspan="3" | Human variants<br />
|-<br />
! Effect || Number || Percent<br />
|-<br />
|Observed functional effect ||23121 ||2%<br />
|-<br />
|Disease associations ||26842 ||3%<br />
|-<br />
|Observed functional effect and disease ||1629 ||0.17%<br />
|-<br />
|Overall ||967879 ||100%<br />
|}<br />
<center><small>'''<caption>''' Statistics about the human variants in SNPdbe. </caption></small></center><br />
</figtable><br />
<br />
Search for Gaucher disease delivered [http://www.rostlab.org/services/snpdbe/dosearch.php?id=disease&val=gaucher 174 entries], 147 from them from the human gene GBA and protein P04062. Note: as some of the mutation occur at the same position, there are only 121 distinct mutated positions from the subset of 147.<br />
<br />
Most SNPs are not validated:<br />
<br />
<figtable id="snpdbe_evidence"><br />
{|class="colBasic2" <br />
! colspan="2" | Experimental evidence<br />
|-<br />
! Number of mutations || Evidence type<br />
|-<br />
| 120 ||Not validated<br />
|-<br />
| 20 ||by cluster<br />
|-<br />
| 3 ||by cluster,freq<br />
|-<br />
| 2 ||1000Genome,freq,cluster<br />
|-<br />
| 1 ||by freq<br />
|-<br />
| 1 ||HapMap,freq,cluster<br />
|}<br />
<center><small>'''<caption>''' Statistics about the experimental evidence in SNPdbe. </caption></small></center><br />
</figtable><br />
<br />
Conservation score: Likelihood of observing either the wt (wildtype; green bar) or mt (mutant; red bar) at given position in the sequence. The longer the bar the higher the likelihood. The conservation score can be used as a first (simple) estimate of effect (disease causing) or no effect. There are three types of conservation scores: Pssm, Perc and Psic. There is a direct correlation between the length of the bars and Perc scores, however it is difficult to find a threshold to discriminate effect from no-effect. On the contrary, Pssm mt scores below 0 combined with Pssm wt scores above 0 imply very low occurrence of the mutant in comparison to the wild type protein, which implies that the mutation is deleterious (causes a negative effect). With this method we received 46 no-effect 101 effect mutations.<br />
<br />
<!-- 12 wt: negative, mt: neutral<br />
3 wt: negative, mt: positive<br />
14 wt: neutral, mt: negative<br />
81 wt: neutral, mt: neutral<br />
13 wt: neutral, mt: positive<br />
2 wt: positive, mt: negative<br />
22 wt: positive, mt: neutral<br />
--><br />
<br />
<!--In SNPdbe, look for disease causing mutations and those that have no effect (often not verified). Please disregard the two colums "Predicted functional effect" for this week.<br />
You can search for example your protein, gene or disease.<br />
Look at results for human.<br />
Is experimental evidence available?<br />
Use the conservation score as a first (simple) estimate of effect (disease causing) or no effect.--><br />
<br />
==OMIM==<br />
<br />
*daily updated<br />
*21 844 entries (updated 11 June 2013)<br />
*gene entries supply information about mutations from GWAs<br />
*allelic variants (only selected mutations)<br />
*disease entries<br />
*relationship between genotype and phenotype (diseases)<br />
*text mining of publications about the gene, allelic variants or disease<br />
<br />
For the ''GBA'' gene 48 mutations are found in OMIM (41 of them are referenced to dbSNP), which is a small number in comparison to the other databases. [http://omim.org/allelicVariant/606463 OMIM table view of GBA allelic variants].<br />
<br />
==SNP Databases Summary==<br />
<br />
<figtable id="databases"><br />
{|class="colBasic2"<br />
! colspan="6" | SNP Databases<br />
|-<br />
! rowspan="2" | Information || colspan="5" | Database<br />
|-<br />
! [http://www.hgmd.org/ HGMD] || [http://www.ncbi.nlm.nih.gov/SNP/ dbSNP] || [http://www.snpedia.com/ SNPedia] || [https://www.rostlab.org/services/snpdbe/ SNPdbe] || [http://omim.org OMIM]<br />
|-<br />
| '''Type of information''' || Only non-silent mutations in the coding region: disease-causing/associated mutations, disease-associated/functional polymorphisms. Mutation type/number/effect, associated disease phenotype, chromosomal location, gene symbol, reference of its first literature report || Mutation information for ''Homo sapiens'', ''Mus musculus'' and ''Bos taurus'': SNPs, short deletion and insertion polymorphisms || Mutation information collected from different databases|| For each SNP: experimentally derived functional and structural impact, predicted functional effect, associated disease, average heterozygosity, experimental evidence of the nsSNP, evolutionary conservation of wildtype and mutant amino acid, link-outs to external databases || Gene entries - information about mutations from GWAs, allelic variants (only selected examples), disease entries, relationship between genotype and phenotype (diseases) <br />
|-<br />
| '''Source of information''' || Literature, reported functional studies || NCBI in collaboration with National Human Genome Research Institute || Various databases (OMIM, dbSNP, 23andME, etc.), searching machines (google, wikipedia, etc.) || Various databases (SwissProt, PMD, dbSNP, 1000 Genomes), SNP effect is predicted with SIFT and SNAP || Full-text, referenced overviews<br />
|-<br />
| '''Last update''' || Professional version: March 2013, free public version: from professional release 2008 || April 25, 2013 || May 16, 2013 || February 20, 2012 || daily updated <br />
|-<br />
| '''GBA mutations''' || Total: 335, of them missense/nonsense SNPs: 256 || Total: 432, of them silent SNPs: 59 || 48 SNPs || Total: 147 SNPs (46 no-effect 101 effect) || At least 48 deleterious SNPs (in "allelic variants" table)<br />
|}<br />
<center><small>'''<caption>''' Summary about the explored SNPs databases: HGMD, dbSNP, SNPedia, dbSNP and OMIM. </caption></small></center><br />
</figtable><br />
<br />
==Mutation map==<br />
We extracted information about the point mutations in our protein ''GBA'' causing Gaucher disease from three different databases described above: HGMD, dbSNP and SNPdbe. For dbSNP, the contig NT_004487.19 was used. We mapped the mutations to the location on the reference protein sequence (NP_001005741.1, identical to the UniProt entry P04062) and combined the information about identical mutations. Three different types of mutations were extracted: <br />
* '''missense''', which lead to amino acid exchange (from all databases)<br />
* '''nonsense''', which lead to a premature termination of translation and end of the protein (only from HGMD)<br />
* '''synonymous''', leading to a different codon, but encoding the same amino acid (only from dbSNP)<br />
<br />
As the next step, the mutations were classified into <br />
*'''pathogenic''' (i.e. Gaucher disease causing) and <br />
*'''not pathogenic''' (non-disease causing). <br />
All mutations from HGMD are pathogenic. In dbSNP, no such annotation is found. In SNPdbe we decided whether a mutation is pathogenic or not according to the conservation scores, as described above. If a mutation had at least one annotation that it causes Gaucher disease, we classified it as pathogenic, otherwise as not pathogenic.<br />
<br />
Altogether 366 point mutations were collected our protein sequence, from them 246 disease causing and 120 non-disease causing (<xr id="mut_map"/>). Finally, we plotted the mutation according to the classes and the types using R (Figure 1). <br />
<br />
<figtable id="mut_map"><br />
{|class="colBasic2"<br />
!AA position || Mutation || Type || Pathogenic || Accession || Disease causing?<br />
|-<br />
|5 || S-N || missense || N/A || rs201985614 || FALSE<br />
|-<br />
|13 || K-R || missense || Gaucher disease ?; N/A || CM065215; rs150466109 || TRUE<br />
|-<br />
|14 || P-S || missense || N/A || rs371238435 || FALSE<br />
|-<br />
|15 || L-S || missense || N/A || rs1141802 || FALSE<br />
|-<br />
|16 || S-G || missense || N/A || rs1141804 || FALSE<br />
|-<br />
|17 || R-G || missense || N/A || rs139626710 || FALSE<br />
|-<br />
|19 || S-N || missense || N/A || rs376644484 || FALSE<br />
|-<br />
|20 || I-V || missense || N/A || rs143187997 || FALSE<br />
|-<br />
|27 || G-G || synonymous || N/A || rs1059731 || FALSE<br />
|-<br />
|28 || L-L || synonymous || N/A || rs201330214 || FALSE<br />
|-<br />
|35 || S-S || synonymous || N/A || rs148001886 || FALSE<br />
|-<br />
|36 || W-TERM || nonsense || Gaucher disease 1 || CM980822 || TRUE<br />
|-<br />
|39 || G-D || missense || N/A || rs200378040 || FALSE<br />
|-<br />
|41 || R-C || missense || N/A || rs190207858 || FALSE<br />
|-<br />
|46 || K-E || missense || N/A || rs142761046 || FALSE<br />
|-<br />
|48 || F-F || synonymous || N/A || rs185884197 || FALSE<br />
|-<br />
|49 || G-S || missense || Gaucher disease || CM081634 || TRUE<br />
|-<br />
|51 || S-I || missense || Gaucher disease || CM057078 || TRUE<br />
|-<br />
|54 || V-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM960691; rs121908302 || TRUE<br />
|-<br />
|54 || V-M || missense || Gaucher disease || CM044630 || TRUE<br />
|-<br />
|55 || C-S || missense || Gaucher disease; gaucher disease (SwissVar) || HM971738; N/A || TRUE<br />
|-<br />
|56 || V-V || synonymous || N/A || rs145773486 || FALSE<br />
|-<br />
|62 || C-Y || missense || N/A || rs145888253 || FALSE<br />
|-<br />
|63 || D-D || synonymous || N/A || rs74953658 || FALSE<br />
|-<br />
|63 || D-E || missense || N/A || rs74953658 || FALSE<br />
|-<br />
|63 || D-N || missense || Gaucher disease 1; gaucher disease type 1 (SwissVar) || CM050263; N/A || TRUE<br />
|-<br />
|68 || P-R || missense || N/A || rs141061530 || FALSE<br />
|-<br />
|72 || A-A || synonymous || N/A || rs151093421 || FALSE<br />
|-<br />
|74 || G-A || missense || N/A || rs371592589 || FALSE<br />
|-<br />
|74 || G-S || missense || Gaucher disease ? || CM065221 || TRUE<br />
|-<br />
|75 || T-N || missense || N/A || rs373363286 || FALSE<br />
|-<br />
|76 || F-F || synonymous || N/A || rs75954905 || FALSE<br />
|-<br />
|76 || F-L || missense || N/A || rs75954905 || FALSE<br />
|-<br />
|76 || F-V || missense || Gaucher disease 1; N/A(gaucher disease (SwissVar)) || CM970615; N/A || TRUE<br />
|-<br />
|77 || S-R || missense || N/A || rs368786234 || FALSE<br />
|-<br />
|78 || R-C || missense || N/A || rs146774384 || FALSE<br />
|-<br />
|80 || E-E || synonymous || N/A || rs76337315 || FALSE<br />
|-<br />
|80 || E-K || missense || Gaucher disease 2; gaucher disease type 2 (SwissVar) || CM983509; rs1141808 || TRUE<br />
|-<br />
|81 || S-N || missense || Gaucher disease || CM081630 || TRUE<br />
|-<br />
|81 || S-S || synonymous || N/A || rs1141810 || FALSE<br />
|-<br />
|82 || T-I || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM950560; rs1141811; rs1141811 || TRUE<br />
|-<br />
|82 || T-R || missense || N/A || rs1141811 || FALSE<br />
|-<br />
|83 || R-C || missense || N/A || rs1141812 || FALSE<br />
|-<br />
|83 || R-S || missense || N/A || rs1141812 || FALSE<br />
|-<br />
|85 || G-E || missense || Gaucher disease; gaucher disease (SwissVar) || CM960692; rs77829017 || TRUE<br />
|-<br />
|86 || R-Q || missense || N/A || rs144173415 || FALSE<br />
|-<br />
|86 || R-TERM || nonsense || Gaucher disease || CM016030 || TRUE<br />
|-<br />
|87 || R-Q || missense || Gaucher disease; N/A; N/A(gaucher disease (SwissVar)) || CM071788; rs78769774; rs78769774 || TRUE<br />
|-<br />
|87 || R-R || synonymous || N/A || rs78669556 || FALSE<br />
|-<br />
|87 || R-W || missense || Gaucher disease; N/A; gaucher disease (SwissVar). In severe Gaucher disease (GD) type 1 (PMD) || CM950561; rs1141814; rs1141814 || TRUE<br />
|-<br />
|92 || M-T || missense || N/A || rs1141815 || FALSE<br />
|-<br />
|93 || G-G || synonymous || N/A || rs1141816 || FALSE<br />
|-<br />
|99 || H-R || missense || N/A || rs1141820 || FALSE<br />
|-<br />
|99 || H-Y || missense || N/A || rs1141818 || FALSE<br />
|-<br />
|100 || T-T || synonymous || N/A || rs1141821 || FALSE<br />
|-<br />
|105 || L-P || missense || Gaucher disease || CM057085 || TRUE<br />
|-<br />
|108 || L-L || synonymous || N/A || rs368145008 || FALSE<br />
|-<br />
|112 || Q-TERM || nonsense || Gaucher disease || CM057075 || TRUE<br />
|-<br />
|113 || K-TERM || nonsense || Gaucher disease || CM970616 || TRUE<br />
|-<br />
|117 || V-A || missense || Gaucher disease || CM081644 || TRUE<br />
|-<br />
|118 || K-N || missense || Gaucher disease; gaucher disease (SwissVar) || CM960693; rs121908312 || TRUE<br />
|-<br />
|124 || M-T || missense || Gaucher disease || CM081632 || TRUE<br />
|-<br />
|129 || A-A || synonymous || N/A || rs79175920 || FALSE<br />
|-<br />
|129 || A-T || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM001161; N/A || TRUE<br />
|-<br />
|141 || N-S || missense || N/A || rs374003673 || FALSE<br />
|-<br />
|144 || L-R || missense || Gaucher disease 1 || CM020940 || TRUE<br />
|-<br />
|146 || S-L || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980823; N/A || TRUE<br />
|-<br />
|147 || Y-C || missense || Gaucher disease || CM082775 || TRUE<br />
|-<br />
|148 || F-V || missense || Gaucher disease 1 || CM005404 || TRUE<br />
|-<br />
|150 || E-K || missense || Gaucher disease ? || CM065220 || TRUE<br />
|-<br />
|152 || G-E || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM980824; N/A || TRUE<br />
|-<br />
|156 || N-D || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM001162; N/A || TRUE<br />
|-<br />
|156 || N-K || missense || N/A || rs77019233 || FALSE<br />
|-<br />
|158 || I-I || synonymous || N/A || rs147411159 || FALSE<br />
|-<br />
|158 || I-S || missense || Gaucher disease 1; N/A; gaucher disease type 1 (SwissVar) || CM050264; rs77834747; rs77834747 || TRUE<br />
|-<br />
|158 || I-T || missense || Gaucher disease; gaucher disease (SwissVar) || CM980825; N/A || TRUE<br />
|-<br />
|159 || R-Q || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM880035; rs79653797 || TRUE<br />
|-<br />
|159 || R-R || synonymous || N/A || rs75249684 || FALSE<br />
|-<br />
|159 || R-W || missense || Gaucher disease; gaucher disease (SwissVar) || CM960694; rs439898 || TRUE<br />
|-<br />
|160 || V-A || missense || Gaucher disease ? || CM057093 || TRUE<br />
|-<br />
|161 || P-L || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM052240; rs79637617; rs79637617 || TRUE<br />
|-<br />
|161 || P-P || synonymous || N/A || rs79767521 || FALSE<br />
|-<br />
|161 || P-S || missense || Gaucher disease; gaucher disease (SwissVar) || CM930289; rs121908299 || TRUE<br />
|-<br />
|162 || M-I || missense || N/A || rs77959976 || FALSE<br />
|-<br />
|162 || M-T || missense || Gaucher disease || CM044627 || TRUE<br />
|-<br />
|162 || M-V || missense || Gaucher disease; N/A; N/A(gaucher disease (SwissVar)) || CM057081; rs377325220; N/A || TRUE<br />
|-<br />
|166 || D-V || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM057089; rs79796061; rs79796061 || TRUE<br />
|-<br />
|170 || R-C || missense || Gaucher disease 2; gaucher disease type 1 gaucher disease type 2 (SwissVar) || CM983510; N/A || TRUE<br />
|-<br />
|170 || R-L || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM000161; rs75690705 || TRUE<br />
|-<br />
|173 || T-I || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM001163; rs78657146 || TRUE<br />
|-<br />
|173 || T-P || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM980826; N/A || TRUE<br />
|-<br />
|174 || Y-TERM || nonsense || Gaucher disease 1 || CM001164 || TRUE<br />
|-<br />
|175 || A-E || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM020710; rs79660787 || TRUE<br />
|-<br />
|179 || D-H || missense || Gaucher disease 1; N/A; gaucher disease (SwissVar) || CM910171; rs147138516; rs147138516 || TRUE<br />
|-<br />
|182 || Q-R || missense || N/A || rs375193074 || FALSE<br />
|-<br />
|191 || E-A || missense || Gaucher disease || CM081639 || TRUE<br />
|-<br />
|196 || K-N || missense || Gaucher disease ? || CM057074 || TRUE<br />
|-<br />
|196 || K-Q || missense || Gaucher disease 2; N/A(gaucher disease (SwissVar)) || CM910172; rs121908297 || TRUE<br />
|-<br />
|198 || P-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM980827; rs80222298 || TRUE<br />
|-<br />
|198 || P-T || missense || Gaucher disease; gaucher disease (SwissVar) || CM022012; N/A || TRUE<br />
|-<br />
|200 || I-N || missense || gaucher disease (SwissVar) || rs77933015 || TRUE<br />
|-<br />
|200 || I-S || missense || Gaucher disease; gaucher disease (SwissVar) || CM980828; rs77933015 || TRUE<br />
|-<br />
|201 || H-P || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM020711; rs76500263 || TRUE<br />
|-<br />
|202 || R-TERM || nonsense || Gaucher disease || CM980829 || TRUE<br />
|-<br />
|208 || Q-TERM || nonsense || Gaucher disease 1 || CM980830 || TRUE<br />
|-<br />
|209 || R-C || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM001165; N/A || TRUE<br />
|-<br />
|209 || R-P || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980831; N/A || TRUE<br />
|-<br />
|210 || P-P || synonymous || N/A || rs201615998 || FALSE<br />
|-<br />
|211 || V-I || missense || N/A || rs188760929 || FALSE<br />
|-<br />
|212 || S-TERM || nonsense || Gaucher disease || CM950562 || TRUE<br />
|-<br />
|213 || L-F || missense || Gaucher disease ?; N/A; N/A(gaucher disease (SwissVar)) || CM057076; rs374591570; N/A || TRUE<br />
|-<br />
|213 || L-L || synonymous || N/A || rs78659905 || FALSE<br />
|-<br />
|215 || A-D || missense || Gaucher disease; gaucher disease (SwissVar) || CM940805; N/A || TRUE<br />
|-<br />
|217 || P-P || synonymous || N/A || rs76717906 || FALSE<br />
|-<br />
|217 || P-S || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM950563; N/A || TRUE<br />
|-<br />
|218 || W-TERM || nonsense || Gaucher disease || CM970617 || TRUE<br />
|-<br />
|219 || T-P || missense || Gaucher disease || CM081641 || TRUE<br />
|-<br />
|221 || P-L || missense || Gaucher disease 2; N/A; gaucher disease type 1 (SwissVar) || CM041347; rs80205046; rs80205046 || TRUE<br />
|-<br />
|221 || P-P || synonymous || N/A || rs76727497 || FALSE<br />
|-<br />
|221 || P-T || missense || Gaucher disease; gaucher disease (SwissVar) || CM940806; N/A || TRUE<br />
|-<br />
|223 || W-R || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM001166; rs61748906; rs61748906 || TRUE<br />
|-<br />
|224 || L-F || missense || Gaucher disease ?; N/A(gaucher disease (SwissVar)) || CM057091; N/A || TRUE<br />
|-<br />
|224 || L-L || synonymous || N/A || rs76682322 || FALSE<br />
|-<br />
|227 || N-K || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM980832; rs381418; rs381418 || TRUE<br />
|-<br />
|227 || N-S || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM960695; rs364897 || TRUE<br />
|-<br />
|228 || G-V || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM970618; rs78911246; rs78911246 || TRUE<br />
|-<br />
|229 || A-A || synonymous || N/A || rs75370695 || FALSE<br />
|-<br />
|229 || A-E || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM000162; rs75636769; rs75636769 || TRUE<br />
|-<br />
|229 || A-T || missense || Gaucher disease 3; N/A(gaucher disease (SwissVar)) || CM001167; N/A || TRUE<br />
|-<br />
|230 || V-E || missense || Gaucher disease 1; N/A; gaucher disease type 1 (SwissVar) || CM050265; rs381427; rs381427 || TRUE<br />
|-<br />
|230 || V-G || missense || Gaucher disease; N/A; gaucher disease type 1 (SwissVar) || CM980833; rs381427; rs381427 || TRUE<br />
|-<br />
|232 || G-E || missense || gaucher disease (SwissVar) || N/A || TRUE<br />
|-<br />
|232 || G-G || synonymous || N/A || rs375731497 || FALSE<br />
|-<br />
|232 || G-W || missense || Gaucher disease || CM092866 || TRUE<br />
|-<br />
|234 || G-E || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM970619; rs74462743; rs74462743 || TRUE<br />
|-<br />
|234 || G-W || missense || Gaucher disease; gaucher disease (SwissVar) || CM990680; N/A || TRUE<br />
|-<br />
|235 || S-P || missense || Gaucher disease; gaucher disease (SwissVar) || CM992893; rs1064644 || TRUE<br />
|-<br />
|235 || S-S || synonymous || N/A || rs76158190 || FALSE<br />
|-<br />
|236 || L-F || missense || Gaucher disease || CM057077 || TRUE<br />
|-<br />
|236 || L-L || synonymous || N/A || rs372785813 || FALSE<br />
|-<br />
|236 || L-P || missense || Gaucher disease ? || CM057087 || TRUE<br />
|-<br />
|237 || K-E || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM020712; N/A || TRUE<br />
|-<br />
|237 || K-K || synonymous || N/A || rs74486098 || FALSE<br />
|-<br />
|240 || P-P || synonymous || N/A || rs376613535 || FALSE<br />
|-<br />
|241 || G-E || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM992894; rs77451368; rs77451368 || TRUE<br />
|-<br />
|241 || G-R || missense || Gaucher disease; N/A(gaucher disease (SwissVar). In severe neuronopathic (type 2) Gaucher's disease: This mutant acid beta-glucosidase persists in the patient's fibroblasts as a mannose-rich polypeptide in the endoplasmic reticulum and is not transported to the lysosomes. (PMD)) || CM940807; N/A || TRUE<br />
|-<br />
|244 || Y-C || missense || Gaucher disease 1; N/A; N/A(gaucher disease (SwissVar)) || CM990681; rs76026102; rs76026102 || TRUE<br />
|-<br />
|248 || W-R || missense || Gaucher disease || CM081637 || TRUE<br />
|-<br />
|249 || A-V || missense || Gaucher disease ? || CM065217 || TRUE<br />
|-<br />
|251 || Y-H || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM930290; rs121908300 || TRUE<br />
|-<br />
|252 || F-C || missense || Gaucher disease || HM971754 || TRUE<br />
|-<br />
|252 || F-I || missense || Gaucher disease 3; N/A(gaucher disease (SwissVar)) || CM910173; rs381737 || TRUE<br />
|-<br />
|252 || F-L || missense || N/A || rs79945741 || FALSE<br />
|-<br />
|255 || F-V || missense || Gaucher disease || CM940808 || TRUE<br />
|-<br />
|255 || F-Y || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM900103; rs74500255 || TRUE<br />
|-<br />
|259 || Y-C || missense || Gaucher disease ? || CM065219 || TRUE<br />
|-<br />
|270 || T-R || missense || Gaucher disease; gaucher disease (SwissVar) || CM022013; rs76725886 || TRUE<br />
|-<br />
|272 || E-D || missense || Gaucher disease || CM081640 || TRUE<br />
|-<br />
|272 || E-TERM || nonsense || Gaucher disease 2 || CM990682 || TRUE<br />
|-<br />
|275 || P-T || missense || Gaucher disease || CM077546 || TRUE<br />
|-<br />
|276 || S-P || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980834; N/A || TRUE<br />
|-<br />
|278 || G-V || missense || Gaucher disease || CM081643 || TRUE<br />
|-<br />
|279 || L-L || synonymous || N/A || rs371576958 || FALSE<br />
|-<br />
|281 || S-N || missense || N/A || rs138246400 || FALSE<br />
|-<br />
|282 || G-V || missense || Gaucher disease || CM081642 || TRUE<br />
|-<br />
|283 || Y-H || missense || Gaucher disease || CM081631 || TRUE<br />
|-<br />
|284 || P-H || missense || Gaucher disease ? || CM057082 || TRUE<br />
|-<br />
|288 || L-Q || missense || Gaucher disease || CM077543 || TRUE<br />
|-<br />
|290 || F-L || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM020713; rs121908313 || TRUE<br />
|-<br />
|294 || H-Q || missense || Gaucher disease 2; N/A(gaucher disease type 1 (SwissVar)) || CM000163; N/A || TRUE<br />
|-<br />
|296 || R-Q || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM940809; rs78973108 || TRUE<br />
|-<br />
|296 || R-TERM || nonsense || Gaucher disease 1 || CM980835 || TRUE<br />
|-<br />
|298 || F-L || missense || Gaucher disease 2; N/A(gaucher disease (SwissVar)) || CM000164; N/A || TRUE<br />
|-<br />
|299 || I-T || missense || Gaucher disease 2 || CM053270 || TRUE<br />
|-<br />
|301 || R-C || missense || N/A || rs374117599 || FALSE<br />
|-<br />
|301 || R-H || missense || N/A || rs140955685 || FALSE<br />
|-<br />
|303 || L-I || missense || N/A(gaucher disease (SwissVar)) || N/A || FALSE<br />
|-<br />
|304 || G-D || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM980836; rs80116658; rs80116658 || TRUE<br />
|-<br />
|305 || P-A || missense || Gaucher disease || CM057095 || TRUE<br />
|-<br />
|305 || P-L || missense || Gaucher disease 2 || HM971737 || TRUE<br />
|-<br />
|305 || P-R || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM950564; rs79215220; rs79215220 || TRUE<br />
|-<br />
|306 || T-I || missense || N/A || rs199628072 || FALSE<br />
|-<br />
|306 || T-T || synonymous || N/A || rs371856161 || FALSE<br />
|-<br />
|308 || A-T || missense || N/A || rs368425393 || FALSE<br />
|-<br />
|310 || S-N || missense || Gaucher disease; N/A; N/A(gaucher disease (SwissVar)) || CM970620; rs74731340; rs74731340 || TRUE<br />
|-<br />
|318 || L-P || missense || Gaucher disease || CM081635 || TRUE<br />
|-<br />
|324 || R-C || missense || Gaucher disease; gaucher disease (SwissVar) || CM940810; N/A || TRUE<br />
|-<br />
|324 || R-H || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM000165; rs79696831; rs79696831 || TRUE<br />
|-<br />
|327 || L-P || missense || Gaucher disease || CM077544 || TRUE<br />
|-<br />
|328 || P-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM920298; rs121908298 || TRUE<br />
|-<br />
|329 || H-Q || missense || N/A || rs142662866 || FALSE<br />
|-<br />
|335 || L-L || missense || Gaucher disease 1 ? || CM080379 || TRUE<br />
|-<br />
|335 || L-V || missense || Gaucher disease 1 || CM082770 || TRUE<br />
|-<br />
|338 || P-A || missense || N/A || rs374463271 || FALSE<br />
|-<br />
|342 || K-I || missense || Gaucher disease; N/A; N/A(gaucher disease (SwissVar)) || CM980837; rs77714449; rs77714449 || TRUE<br />
|-<br />
|342 || K-K || synonymous || N/A || rs1064646 || FALSE<br />
|-<br />
|343 || Y-C || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM000166; rs77321207; rs77321207 || TRUE<br />
|-<br />
|343 || Y-TERM || nonsense || Gaucher disease 3 || CM001168 || TRUE<br />
|-<br />
|344 || V-L || missense || Gaucher disease ? || CM057098 || TRUE<br />
|-<br />
|345 || H-Y || missense || N/A || rs181720335 || FALSE<br />
|-<br />
|346 || G-G || synonymous || N/A || rs1064647 || FALSE<br />
|-<br />
|348 || A-V || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM910174; rs78396650 || TRUE<br />
|-<br />
|349 || V-I || missense || N/A || rs371083513 || FALSE<br />
|-<br />
|350 || H-R || missense || Gaucher disease 2 || CM993703 || TRUE<br />
|-<br />
|351 || W-C || missense || Gaucher disease 1; N/A(gaucher disease (SwissVar)) || CM910175; rs121908304 || TRUE<br />
|-<br />
|351 || W-R || missense || Gaucher disease || CM074886 || TRUE<br />
|-<br />
|352 || Y-H || missense || Gaucher disease; gaucher disease (SwissVar) || CM960696; N/A || TRUE<br />
|-<br />
|352 || Y-Y || synonymous || N/A || rs79311125 || FALSE<br />
|-<br />
|354 || D-H || missense || Gaucher disease; gaucher disease (SwissVar) || CM950565; N/A || TRUE<br />
|-<br />
|357 || A-D || missense || Gaucher disease; N/A; N/A(gaucher disease (SwissVar)) || CM950566; rs78188205; rs78188205 || TRUE<br />
|-<br />
|358 || P-A || missense || Gaucher disease ? || CM057094 || TRUE<br />
|-<br />
|358 || P-S || missense || Gaucher disease || CM077547 || TRUE<br />
|-<br />
|362 || T-I || missense || Gaucher disease; gaucher disease (SwissVar) || CM920299; rs76539814 || TRUE<br />
|-<br />
|363 || L-P || missense || Gaucher disease; gaucher disease (SwissVar) || CM980838; N/A || TRUE<br />
|-<br />
|363 || L-Q || missense || Gaucher disease 1 || CM076208 || TRUE<br />
|-<br />
|364 || G-G || synonymous || N/A || rs143222798 || FALSE<br />
|-<br />
|364 || G-R || missense || Gaucher disease 2; N/A(gaucher disease (SwissVar)) || CM900104; rs121908305 || TRUE<br />
|-<br />
|364 || G-W || missense || Gaucher disease || CM014190 || TRUE<br />
|-<br />
|365 || E-E || synonymous || N/A || rs80317710 || FALSE<br />
|-<br />
|365 || E-K || missense || Gaucher disease 1 ?; N/A(gaucher disease (SwissVar)) || CM910176; rs2230288 || TRUE<br />
|-<br />
|368 || R-C || missense || N/A || rs374306700 || FALSE<br />
|-<br />
|368 || R-H || missense || N/A || rs1064648 || FALSE<br />
|-<br />
|370 || F-S || missense || Gaucher disease || CM081647 || TRUE<br />
|-<br />
|375 || L-P || missense || Gaucher disease || CM044629 || TRUE<br />
|-<br />
|380 || A-T || missense || Gaucher disease; gaucher disease (SwissVar) || CM980839; N/A || TRUE<br />
|-<br />
|381 || C-G || missense || Gaucher disease 2; N/A(gaucher disease (SwissVar)) || CM900105; rs121908306 || TRUE<br />
|-<br />
|381 || C-R || missense || Gaucher disease 1 || CM025357 || TRUE<br />
|-<br />
|381 || C-Y || missense || Gaucher disease || CM057097 || TRUE<br />
|-<br />
|387 || W-G || missense || Gaucher disease || CM081638 || TRUE<br />
|-<br />
|388 || E-E || synonymous || N/A || rs75391747 || FALSE<br />
|-<br />
|388 || E-K || missense || Gaucher disease ?; gaucher disease (SwissVar) || CM057092; N/A || TRUE<br />
|-<br />
|389 || Q-TERM || nonsense || Gaucher disease 1 || CM020714 || TRUE<br />
|-<br />
|391 || V-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM970621; N/A || TRUE<br />
|-<br />
|391 || V-M || missense || Gaucher disease || CM077548 || TRUE<br />
|-<br />
|392 || R-G || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980840; rs121908308 || TRUE<br />
|-<br />
|392 || R-Q || missense || N/A || rs11558184 || FALSE<br />
|-<br />
|392 || R-W || missense || Gaucher disease ?; gaucher disease (SwissVar) || CM057090; N/A || TRUE<br />
|-<br />
|394 || G-D || missense || Gaucher disease || HM971755 || TRUE<br />
|-<br />
|395 || S-F || missense || Gaucher disease 1 || HM971756 || TRUE<br />
|-<br />
|396 || W-C || missense || Gaucher disease || CM093532 || TRUE<br />
|-<br />
|396 || W-TERM || nonsense || Gaucher disease || CM043998 || TRUE<br />
|-<br />
|398 || R-Q || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM920300; rs74979486; rs74979486 || TRUE<br />
|-<br />
|398 || R-TERM || nonsense || Gaucher disease || CM940811 || TRUE<br />
|-<br />
|400 || M-I || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM022014; rs149487315; rs149487315 || TRUE<br />
|-<br />
|402 || Y-C || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM052241; rs76228122; rs76228122 || TRUE<br />
|-<br />
|403 || S-N || missense || Gaucher disease || CM074884 || TRUE<br />
|-<br />
|403 || S-R || missense || Gaucher disease || CM044628 || TRUE<br />
|-<br />
|403 || S-T || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM910177; rs121908307 || TRUE<br />
|-<br />
|405 || S-G || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM970622; N/A || TRUE<br />
|-<br />
|405 || S-N || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980841; N/A || TRUE<br />
|-<br />
|405 || S-R || missense || N/A || rs75528494 || FALSE<br />
|-<br />
|405 || S-T || missense || Gaucher disease || HM971759 || TRUE<br />
|-<br />
|408 || T-M || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM960697; rs75548401; rs2230289 || TRUE<br />
|-<br />
|408 || T-T || synonymous || N/A || rs138498426 || FALSE<br />
|-<br />
|409 || N-K || missense || Gaucher disease ? || CM057073 || TRUE<br />
|-<br />
|409 || N-S || missense || Gaucher disease 1; N/A(gaucher disease type 1 (SwissVar)) || CM880036; rs76763715 || TRUE<br />
|-<br />
|410 || L-L || synonymous || N/A || rs74498117 || FALSE<br />
|-<br />
|410 || L-V || missense || Gaucher disease; gaucher disease (SwissVar) || CM040422; rs121908314 || TRUE<br />
|-<br />
|414 || V-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM970623; N/A || TRUE<br />
|-<br />
|416 || G-G || synonymous || N/A || rs75034092 || FALSE<br />
|-<br />
|416 || G-S || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM940812; rs121908311 || TRUE<br />
|-<br />
|417 || W-C || missense || N/A || rs76014919 || FALSE<br />
|-<br />
|417 || W-G || missense || Gaucher disease; gaucher disease (SwissVar) || CM940813; N/A || TRUE<br />
|-<br />
|417 || W-TERM || nonsense || Gaucher disease || CM057096 || TRUE<br />
|-<br />
|419 || D-A || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM930291; rs77284004; rs77284004 || TRUE<br />
|-<br />
|419 || D-E || missense || N/A || rs78715199 || FALSE<br />
|-<br />
|419 || D-H || missense || Gaucher disease; gaucher disease (SwissVar) || CM054757; N/A || TRUE<br />
|-<br />
|419 || D-N || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM940814; N/A || TRUE<br />
|-<br />
|419 || D-Y || missense || Gaucher disease || CM082774 || TRUE<br />
|-<br />
|420 || W-TERM || nonsense || Gaucher disease || CM081645 || TRUE<br />
|-<br />
|421 || N-K || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM052242; N/A || TRUE<br />
|-<br />
|422 || L-I || missense || N/A || rs187143994 || FALSE<br />
|-<br />
|422 || L-R || missense || Gaucher disease 2 || CM052243 || TRUE<br />
|-<br />
|423 || A-D || missense || Gaucher disease || CM077545 || TRUE<br />
|-<br />
|424 || L-P || missense || Gaucher disease 2 || CM052244 || TRUE<br />
|-<br />
|426 || P-L || missense || Gaucher disease; gaucher disease (SwissVar) || CM960698; N/A || TRUE<br />
|-<br />
|426 || P-P || synonymous || N/A || rs201499639 || FALSE<br />
|-<br />
|427 || E-K || missense || N/A || rs149171124 || FALSE<br />
|-<br />
|427 || E-TERM || nonsense || Gaucher disease || CM057083 || TRUE<br />
|-<br />
|428 || G-E || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM980842; N/A || TRUE<br />
|-<br />
|429 || G-R || missense || Gaucher disease; gaucher disease (SwissVar) || CM057079; N/A || TRUE<br />
|-<br />
|430 || P-L || missense || Gaucher disease 1; N/A; gaucher disease (SwissVar) || CM980843; rs76910485; rs76910485 || TRUE<br />
|-<br />
|431 || N-I || missense || Gaucher disease 2; N/A; gaucher disease (SwissVar) || CM980844; rs77738682; rs77738682 || TRUE<br />
|-<br />
|432 || W-L || missense || Gaucher disease || CM081646 || TRUE<br />
|-<br />
|432 || W-R || missense || Gaucher disease; gaucher disease (SwissVar) || CM980845; N/A || TRUE<br />
|-<br />
|433 || V-L || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM890054; rs80356769 || TRUE<br />
|-<br />
|434 || R-C || missense || Gaucher disease 1 || CM065218 || TRUE<br />
|-<br />
|434 || R-P || missense || Gaucher disease 1 || CM005403 || TRUE<br />
|-<br />
|435 || N-T || missense || Gaucher disease; N/A; gaucher disease type 1 (SwissVar) || CM960699; rs75385858; rs75385858 || TRUE<br />
|-<br />
|436 || F-S || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM057099; rs75243000; rs75243000 || TRUE<br />
|-<br />
|437 || V-F || missense || Gaucher disease 2 || CM993704 || TRUE<br />
|-<br />
|437 || V-I || missense || Gaucher disease 1 || CM065222 || TRUE<br />
|-<br />
|437 || V-L || missense || Gaucher disease 3; N/A(gaucher disease type 3 (SwissVar)) || CM960700; N/A || TRUE<br />
|-<br />
|437 || V-V || synonymous || N/A || rs79032178 || FALSE<br />
|-<br />
|438 || D-D || synonymous || N/A || rs75090908 || FALSE<br />
|-<br />
|438 || D-E || missense || N/A || rs75090908 || FALSE<br />
|-<br />
|438 || D-N || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM940815; N/A || TRUE<br />
|-<br />
|438 || D-Y || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM001169; N/A || TRUE<br />
|-<br />
|440 || P-L || missense || Gaucher disease 1; N/A(gaucher disease type 1 (SwissVar)) || CM990683; rs74598136 || TRUE<br />
|-<br />
|441 || I-F || missense || Gaucher disease 3; gaucher disease (SwissVar) || CM020715; N/A || TRUE<br />
|-<br />
|441 || I-T || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM970624; rs75564605; rs75564605 || TRUE<br />
|-<br />
|444 || D-V || missense || Gaucher disease || CM093533 || TRUE<br />
|-<br />
|446 || T-N || missense || N/A || rs202221385 || FALSE<br />
|-<br />
|448 || D-E || missense || N/A || rs78802049 || FALSE<br />
|-<br />
|448 || D-G || missense || Gaucher disease || CM057088 || TRUE<br />
|-<br />
|448 || D-H || missense || Gaucher disease 2; gaucher disease (SwissVar). Gaucher disease (PMD) || CM900106; rs1064651 || TRUE<br />
|-<br />
|448 || D-V || missense || Gaucher disease; gaucher disease (SwissVar) || CM940816; rs77369218 || TRUE<br />
|-<br />
|450 || F-I || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM980846; N/A || TRUE<br />
|-<br />
|450 || F-L || missense || N/A || rs77035024 || FALSE<br />
|-<br />
|451 || Y-H || missense || Gaucher disease 1; N/A(gaucher disease (SwissVar)) || CM980847; N/A || TRUE<br />
|-<br />
|451 || Y-Y || synonymous || N/A || rs78346899 || FALSE<br />
|-<br />
|452 || K-Q || missense || Gaucher disease; gaucher disease (SwissVar) || CM970625; N/A || TRUE<br />
|-<br />
|453 || Q-R || missense || Gaucher disease || CM057080 || TRUE<br />
|-<br />
|453 || Q-TERM || nonsense || Gaucher disease || CM960701 || TRUE<br />
|-<br />
|454 || P-R || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM890055; rs121908295 || TRUE<br />
|-<br />
|455 || M-I || missense || N/A || rs80020805 || FALSE<br />
|-<br />
|455 || M-V || missense || Gaucher disease 2; gaucher disease (SwissVar) || CM052245; N/A || TRUE<br />
|-<br />
|456 || F-F || synonymous || N/A || rs79185870 || FALSE<br />
|-<br />
|456 || F-L || missense || N/A || rs79185870 || FALSE<br />
|-<br />
|456 || F-V || missense || Gaucher disease 1; gaucher disease (SwissVar) || CM940817; N/A || TRUE<br />
|-<br />
|457 || Y-C || missense || Gaucher disease; N/A; gaucher disease (SwissVar) || CM940818; rs74752878; rs74752878 || TRUE<br />
|-<br />
|460 || G-D || missense || Gaucher disease 1; gaucher disease type 1 (SwissVar) || CM050266; N/A || TRUE<br />
|-<br />
|464 || K-E || missense || Gaucher disease; gaucher disease (SwissVar) || CM920301; N/A || TRUE<br />
|-<br />
|464 || K-N || missense || N/A || rs79226895 || FALSE<br />
|-<br />
|465 || F-V || missense || Gaucher disease || CM085444 || TRUE<br />
|-<br />
|471 || Q-H || missense || N/A || rs12747811 || FALSE<br />
|-<br />
|472 || R-G || missense || Gaucher disease || CM970626 || TRUE<br />
|-<br />
|472 || R-S || missense || Gaucher disease || CM081629 || TRUE<br />
|-<br />
|479 || Q-P || missense || N/A || rs144389406 || FALSE<br />
|-<br />
|483 || L-L || synonymous || N/A || rs369966551 || FALSE<br />
|-<br />
|483 || L-P || missense || Gaucher disease 2; gaucher disease type 1 gaucher disease type 2 (SwissVar). In Gaucher disease (GD) (PMD) || CM870010; rs421016 || TRUE<br />
|-<br />
|483 || L-R || missense || Gaucher disease; gaucher disease (SwissVar) || CM940819; N/A || TRUE<br />
|-<br />
|485 || A-A || synonymous || N/A || rs199928507 || FALSE<br />
|-<br />
|485 || A-P || missense || Gaucher disease; gaucher disease (SwissVar) || CM980848; N/A || TRUE<br />
|-<br />
|490 || H-L || missense || N/A || rs76071730 || FALSE<br />
|-<br />
|490 || H-P || missense || N/A || rs76071730 || FALSE<br />
|-<br />
|490 || H-R || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM022015; rs76071730 || TRUE<br />
|-<br />
|491 || P-P || synonymous || N/A || rs149257166 || FALSE<br />
|-<br />
|495 || A-A || synonymous || N/A || rs375973565 || FALSE<br />
|-<br />
|495 || A-P || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM900107; rs368060 || TRUE<br />
|-<br />
|498 || V-V || synonymous || N/A || rs371779859 || FALSE<br />
|-<br />
|499 || V-L || missense || N/A || rs369068553 || FALSE<br />
|-<br />
|499 || V-M || missense || Gaucher disease 1 || HM971757 || TRUE<br />
|-<br />
|499 || V-V || synonymous || N/A; N/A || rs421050; rs1135675 || FALSE<br />
|-<br />
|500 || L-L || synonymous || N/A || rs189380051 || FALSE<br />
|-<br />
|500 || L-P || missense || Gaucher disease ?; gaucher disease (SwissVar) || CM057086; N/A || TRUE<br />
|-<br />
|501 || N-K || missense || Gaucher disease; gaucher disease (SwissVar) || CM972851; N/A || TRUE<br />
|-<br />
|501 || N-S || missense || Gaucher disease ? || CM057072 || TRUE<br />
|-<br />
|502 || R-C || missense || Gaucher disease; gaucher disease (SwissVar) || CM900108; rs80356771 || TRUE<br />
|-<br />
|502 || R-H || missense || Gaucher disease || CM074885 || TRUE<br />
|-<br />
|502 || R-P || missense || Gaucher disease ?; N/A(gaucher disease (SwissVar)) || CM057084; N/A || TRUE<br />
|-<br />
|504 || S-F || missense || N/A || rs150246414 || FALSE<br />
|-<br />
|507 || V-M || missense || N/A || rs370349398 || FALSE<br />
|-<br />
|509 || L-H || missense || N/A || rs113825752 || FALSE<br />
|-<br />
|509 || L-R || missense || N/A || rs113825752 || FALSE<br />
|-<br />
|513 || D-D || synonymous || N/A || rs77409925 || FALSE<br />
|-<br />
|513 || D-E || missense || N/A || rs77409925 || FALSE<br />
|-<br />
|513 || D-Y || missense || Gaucher disease 2; gaucher disease type 2 (SwissVar) || CM980849; N/A || TRUE<br />
|-<br />
|517 || G-S || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM930292; rs121908301 || TRUE<br />
|-<br />
|519 || L-P || missense || Gaucher disease || CM081636 || TRUE<br />
|-<br />
|527 || S-P || missense || Gaucher disease 2 || CM004522 || TRUE<br />
|-<br />
|528 || I-T || missense || Gaucher disease ? || CM065216 || TRUE<br />
|-<br />
|530 || T-I || missense || Gaucher disease 3; gaucher disease type 3 (SwissVar) || CM960702; rs78016673 || TRUE<br />
|-<br />
|534 || R-C || missense || N/A || rs146519305 || FALSE<br />
|-<br />
|535 || R-C || missense || Gaucher disease; gaucher disease (SwissVar) || CM920302; N/A || TRUE<br />
|-<br />
|535 || R-H || missense || Gaucher disease; N/A(gaucher disease (SwissVar)) || CM930293; rs75822236 || TRUE<br />
|}<br />
<center><small>'''<caption>''' Information about the point mutations of the GBA gene/protein extracted from the HGMD, dbSNP and SNPdbe databases. The mutations are divided into Gaucher disease causing (dc) or non-disease causing (non-dc), as well as into missense, nonsense and synonymous.</caption></small></center><br />
</figtable><br />
<br />
<figure id="mutation_map" ><br />
[[Image:combined_gba_gaucher_mutations.txt.png|thumb|none|1000px|'''<caption>'''The extracted point mutations from Table 7 mapped onto the GBA protein sequence with R. The mutations are divided into Gaucher disease causing (dc) or non-disease causing (non-dc), as well as into missense, nonsense and synonymous.</caption>]]<br />
</figure><br />
<br />
As expected, all synonymous mutations are not disease causing, because they do not change the amino acid. And most nonsense mutations are disease causing, because a premature termination usually results in a shortened and dis-functional protein. In our case even all the nonsense mutations are deleterious. Missense mutation can have a negative effect or not, for our protein most of them do.<br />
<br />
It can be seen in <xr id="mutation_map"/> that there are some mutation hot-spots, i.e. many mutations concentrated in some area of the sequence. For example, around the positions 150 and 200-250 there are disease causing mutation hot-spots. The longest hot-spot with the most disease causing mutations lies in the area between the positions 400 and 450.<br />
<br />
==References==<br />
[http://www.ncbi.nlm.nih.gov/books/NBK21088/ Adrienne Kitts and Stephen Sherry. The Single Nucleotide Polymorphism Database (dbSNP) of Nucleotide Sequence Variation. Chapter 5 in: The NCBI Handbook. Created: October 9, 2002; Last Update: February 2, 2011] <br />
<br />
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278761/ Christian Schaefer, Alice Meier, Burkhard Rost, Yana Bromberg (2012). SNPdbe: Constructing an nsSNP functional impacts database. Bioinformatics 28(4):601-602. ]<br />
<br />
[http://omim.org/help/faq#1.4 OMIM FAQs]<br />
<br />
[http://www.genenames.org/data/hgnc_data.php?hgnc_id=4177 genenames.org report about GBA]</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_10_-_Normal_mode_analysis&diff=38909
Gaucher Disease: Task 10 - Normal mode analysis
2013-09-06T13:01:03Z
<p>Kalemanovm: </p>
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[[Gaucher Disease: Task 10 - Lab Journal|Lab journal]]<br />
<br />
With normal mode analysis (NMA), you are able to simulate or analyze the natural resonant globular movements of proteins. There are two different web-servers available to do a NMA, [http://apps.cbu.uib.no/webnma/home WEBnm@] and [http://www.igs.cnrs-mrs.fr/elnemo/start.html ElNemo]. Both base on elastic network models (ENM). They provide the following information: <br />
<br />
'''WEBnm@'''<br />
* deformation energy<br />
* atomic fluctuations<br />
* 2D correlation matrix of residue movement<br />
* atomic displacement<br />
<br />
'''ElNemo'''<br />
* '''frequency''' of a mode (normalized relative to the lowest mode frequency)<br />
* '''collectivity''' of a mode (what part of the atoms move together)<br />
* normal modes (PDB files) and their animations (in GIF format) of the top five modes<br />
* '''RMSD''' with respect to the second reference structure (if given)<br />
* 2D matrices of distance fluctuations between all C-alpha atom pairs ('''CA-vari''')<br />
* visualization of the mean square displacement of all C-alpha atoms associated with a given mode ('''<R2>''')<br />
* distance variation between successive pairs of CA atoms in the two extreme conformations that were computed for this mode (DQMIN/DQMAX) (still an experimental feature '''CA-strain''')<br />
<br />
==WEBnm@==<br />
WEBnm@ uses only alpha carbon atoms in the models for the normal mode analysis. The number of calculated modes depends on the residue number. In case of less than 1200 residues, 200 modes are calculated for the protein. Otherwise, the number of calculated modes corresponds to only 1/6 of the sequence length modes. As the first six modes refer to mode configurations, the best mode with the lowest frequency is mode 7. The deformation energy is calculated for the 14 best modes (mode 7-20). However, all other features provided by webnm@ are only available for the modes 7-12. <br />
<br />
<br />
===Movements===<br />
<figtable id="energy"><br />
{| border="1" cellpadding="4" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Mode<br />
! style="background:#adceff;" | Deformation Energy<br />
! style="background:#adceff;" | Visualisation<br />
! style="background:#adceff;" | Movements<br />
|-<br />
|7 ||174.43 ||[[Image:m7.png|thumb|300x300px|Mode 7]]|| This mode is hinge-moving with a little twist in the area of both chains linked to each other. Compared to chain B, chain A is more flexible.<br />
|- <br />
|8 || 251.61||[[Image:m8.png|thumb|300x300px|Mode 8]]|| Mode 8 has a stronger hinge movement. The movement directions of both chains are diagonal. According to this directions they hinge together.<br />
|- <br />
|9 || 344.99 ||[[Image:m9.png|thumb|300x300px|Mode 9]]|| Both chains twist around a vertical axis in different directions.<br />
|- <br />
|10 || 759.13 ||[[Image:m10.png|thumb|300x300px|Mode 10]]|| The whole structure is breathing. Chain B seems to be a bit more flexible in its breathing, than the lower one. The breath happens on slightly shifted axis.<br />
|- <br />
|11 || 1099.96 ||[[Image:m11.png|thumb|300x300px|Mode 11]]|| The chains are breathing. But in contrary to mode 10, the breathing is on a vertical axis.<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Five best values of the deformation energy for the lowest-frequency non-trivial modes. In the mode visualization the lower chain is chain A.</caption></small></center><br />
</figtable><br />
<br />
===Domains===<br />
<figure id="reso"><br />
[[Image:resolution.png|thumb|right|300x300px|'''Figure 1: ''' Motions of correlated residues of glucocerebrosidase. The squares at the bottom left and at the top right show the correlated motions within a chain. The two other squares represents motions between the chains. Also the domains can be seen very clear, as residues of the same domain have in general a higher movement correlation to each other than to residues of other chains.]]<br />
</figure><br />
<figure id="struc"><br />
[[Image:struc.png|thumb|right|300x300px|'''Figure 2: '''Visualization of the domains.]]<br />
</figure><br />
<figure id="fluc"><br />
[[Image:fluctuation.png|thumb|right|200x200px|'''Figure 3: '''Fluctuations of the residues. Residues of chain B start at position 496. The square of the fluctuation of each C-alpha atom (for all non-trivial modes), normalized so that the sum over all residues is equal to 100. [http://apps.cbu.uib.no/webnma/jobs/2013-09-04-08-58-31-730213/atomic_displacement]]]<br />
</figure><br />
<br />
The glucocerebrosidase shows a domain definition according to their chains. Within the chains, which are identical, a very big domain and a small domain can be seen (<xr id="struc"/>). The domains can also be identified by their movements in a correlated motion map (<xr id="reso"/>). In <xr id="reso"/> the big domains are centered in each chain. The smaller one consists of two parts in the sequence, which are located before and after the residues of the big domain. A correlation of motions between residues at the beginning and the end of a chain can be observed. In 3D space those two correlating parts are formed together to a bunch of sheets. In contrary, the big domain consists of a high number of helices and a few sheets. All domain databases agree with each other that the bigger domain has a glycosidic characteristics. However, they differ in their information about the second domain. While Pfam neglects a second domain, CATH classifies it as an alpha-mannosidase II and SCOP identifies it as a beta-glycanases. Between the chains there is only a slight movement correlation observable. Some residues of the big domain in one chain correlate with a few other residues at the beginning and outside the small domain of the other chain. The two chains are connected by those residue contacts.<br />
<br />
As chain A and B are identical, the following domain definition concentrates on one chain:<br />
<br />
'''Pfam'''<br />
* 1 domain <br />
* [http://pfam.sanger.ac.uk/family/PF02055 PF02055]: glycoside hydrolase family 30<br />
<br />
'''CATH'''<br />
* 2 domains<br />
* [http://www.cathdb.info/version/latest/superfamily/3.20.20.10 3.20.20.10]: glycosidases<br />
* [http://www.cathdb.info/version/latest/superfamily/2.60.40.1180 2.60.40.1180]: alpha-mannosidase II<br />
'''SCOP'''<br />
* 2 domains<br />
* [http://scop.mrc-lmb.cam.ac.uk/scop/data/scop.b.c.bcb.b.c.b.html b.71.1.2]: composite domain of glycosyl hydrolase families 5, 30, 39 and 51<br />
* [http://scop.mrc-lmb.cam.ac.uk/scop/data/scop.b.d.b.j.c.df.html c.1.8.3]: beta-glycanases<br />
<br />
The fluctuation map (<xr id="fluc"/>) represents the sum of the displacements in each mode weighted by the inverse of their corresponding eigenvalues. Displaced regions can be seen by high fluctuation values. In <xr id="fluc"/> we can observe a very high isolated peak between both chains. This peak identifies a local flexibility between the chains which is at the same time the most flexible part of the protein.<br />
<br />
==ElNemo==<br />
ElNemo uses also only the C-alpha atoms of the structure for normal mode analysis, therefore it takes only the ATOM record from the PDB file, ignoring the HETATM record of a ligand. We ran ElNemo with our reference structure 1OGS without changing anything, so that the ligand could be ignored for the normal mode calculation.<br />
<br />
For the normal mode analysis we specified a cutoff of 10 &Aring; between the C-alpha atoms. A hundred modes were calculated, sorted with rising frequency and numbered from 7 to 106, because the fist 6 lowest frequency modes are trivial ()have low complexity) and omitted ([http://www.igs.cnrs-mrs.fr/elnemo/nmode.cgi?TASK=ANALYSIS&ID=13090400220019046 <R2>, frequency and collectivity information of all found modes]), however, <R2> (see the feature list at the beginning of the page) were computed only for the first 50 modes. For the best non-trivial five modes (7-11), i.e. with lowest frequency, more information is provided, including PDB files and animations in GIF format. B-factor analysis of ElNemo server yielded correlation of 0.662 for 1006 C-alpha atoms ([http://www.igs.cnrs-mrs.fr/elnemo/nmode.cgi?TASK=RUN&ID=13090400220019046 main result page]). The best five modes are presented in <xr id="elnemo_modes"/>. <br />
<br />
<figtable id="elnemo_modes"><br />
{|class="colBasic2"<br />
! rowspan="2"|Mode || rowspan="2"|Frequency || rowspan="2"|Collectivity || colspan="3"|Animations || rowspan="2" style="width:25%"|Description<br />
|-<br />
! a || b || c<br />
|-<br />
|7 || 1.00 || 0.7546 || [[Image:mode7_1.gif]] || [[Image:mode7_2.gif]] || [[Image:mode7_3.gif]] || Hinge motion of the two identical chains.<br />
|- <br />
|8 || 1.22 || 0.7472 || [[Image:mode8_1.gif]] || [[Image:mode8_2.gif]] || [[Image:mode8_3.gif]] || Twist motion of the both chains in opposite directions to the axis which connects the chains combined with a slight bending movement between the two domains (described in the WEBnm@ section) in each chain.<br />
|- <br />
|9 || 1.74 || 0.7765 || [[Image:mode9_1.gif]] || [[Image:mode9_2.gif]] || [[Image:mode9_3.gif]] || Twist motion of the both chains in opposite directions to the axis which connects the chains, similar to that in mode 8 (but no bending between the domains).<br />
|- <br />
|10 || 3.36 || 0.7970 || [[Image:mode10_1.gif]] || [[Image:mode10_2.gif]] || [[Image:mode10_3.gif]] || Breathing of the chains and a slight bending movement between the two domains in each chain.<br />
|- <br />
|11 || 4.30 || 0.8301 || [[Image:mode11_1.gif]] || [[Image:mode11_2.gif]] || [[Image:mode11_3.gif]] || Movement of the chains towards each other and back in the perpendicular direction to the axis connecting them, combined with a slight twist, similar to that in modes 8 and 9. A slight breathing and a hinge movement between the two domains in each chain, similar to that in mode 10, is detectable.<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Five lowest-frequency modes for the structure 1OGS created by ElNemo (cutoff used to identify elastic interactions=10).</caption></small></center><br />
</figtable><br />
<br />
As we can see from the animations of the best five modes, the most flexible regions are where the two chains connect to each other (make a twist, a hinge and a movement towards each other and back on perpendicular from the chain connecting axis) and inside the chains the regions between the two domains (SCOP and CATH) are most flexible (produce a sort of a hinge movement). Other regions inside the domains seem to be stable.<br />
<br />
==Discussion==<br />
As mentioned, both servers calculate the normal modes from the C-alpha atoms of a structure only. In principle, '''3N-6''' normal modes could be calculated, where N is the number of atoms in the protein molecule. This number the be explained very simply. Each atom has three coordinates in the space and thus 3N degrees of freedom. However, to account for rotation symmetry and translation, two times 3 degrees of freedom must be subtracted. As the ''GBA'' protein has 536 residues and thus 536 C-alpha atoms, 1602 normal modes can be computed. However, it is very calculation intensive to find all of them. In normal mode analysis we are interested only in slow, "big range" movements on the chain and domain levels, therefore only few low frequency normal modes are of interest.<br />
<br />
As we do not have a structure completely without a ligand, but only structures with different, not native ligands. The reason for is that the complex with the native ligand, glucosylceramide, is too unstable and the enzyme processes the glucosylceramide instantly into the substrates, ceramide and glucose. Therefore, unfortunately we do not have structures with different conformations for glucocerebrosidase. For this reason, we could not do the option of calculation of normal modes with two different structures. Simply deleting of a ligand from the PDB structure would not result in a change of the main chain coordinates and thus have no effect on the normal modes.<br />
<br />
<!--TODO: Can you observe notable differences between the normal modes calculated by the different servers? <br/>--><br />
ElNemo does not compute a deformation energy, like WEBnm@. Moreover, WEBnm@ gives more information on residue flexibility. Altogether, the modes computed by the both servers describe very similar motions.<br />
<br />
Unlike molecular dynamics (MD), NMA can only compute slow, globular and harmonic motions of a protein. Nevertheless, NMA can help to understand protein motions on the domain and inter-chain level with a comparatively low computational cost. This is a great advantage over the much more time consuming MD. However, NMA only takes the C-alpha atoms into account, thus only main chain movements can influence normal modes, whereas MD accounts for the movements of all atoms in a molecule. Thus, MD can simulate the movements of side chains and thus account for an influence of each SNP, whereas NMA can only notice the effect of an SNP, if it changes the location of the C-alpha atom.<br />
<br />
==Sources==<br />
'''WEBnm@:''' <br/><br />
# [http://apps.cbu.uib.no/webnma/home WEBnm@ server] <br/><br />
# Siv Midtun Hollup, Gisle Salensminde & Nathalie Reuter. (2005) WEBnm@: a web application for normal mode analyses of proteins. BMC Bioinformatics 6: 52. <br />
<br />
'''ElNemo:''' <br/><br />
# [http://www.igs.cnrs-mrs.fr/elnemo ElNemo server] <br/><br />
# K. Suhre & Y.H. Sanejouand, ElNemo: a normal mode web-server for protein movement analysis and the generation of templates for molecular replacement. Nucleic Acids Research, 32, W610-W614, 2004. <br/><br />
# K. Suhre & Y.H. Sanejouand, On the potential of normal mode analysis for solving difficult molecular replacement problems. Acta Cryst. D vol.60, p796-799, 2004 © International Union of Crystallography. <br/></div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_08_-_Sequence-based_mutation_analysis&diff=38908
Gaucher Disease: Task 08 - Sequence-based mutation analysis
2013-09-06T12:59:57Z
<p>Kalemanovm: </p>
<hr />
<div>[[Gaucher_Disease:_Task_08_-_LabJournal|Lab journal]]<br />
<br />
==Mutation Set==<br />
<figtable id="sele"><br />
{| border="1" cellpadding="6" cellspacing="0" align="center" <br />
|-<br />
! colspan="6" style="background:#adceff;" | Mutations<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | mRNA<br />
! colspan="3" style="background:#adceff;" | Protein<br />
|-<br />
! style="background:#efefef;" | Reference<br />
! style="background:#efefef;" | Sequence Position<br />
! style="background:#efefef;" | Codon change <br />
! style="background:#efefef;" | Codon Number<br />
! style="background:#efefef;" | Amino Acid change<br />
! style="background:#efefef;" | One letter code<br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=368786234 rs368786234] || 656 || AG<span style="color:#FF0040">'''C'''</span> ⇒ AG<span style="color:#FF0040">'''A'''</span> || 77 || Ser ⇒ Arg || S77R <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374003673 rs374003673] || 847 || A<span style="color:#FF0040">'''A'''</span>T ⇒ A<span style="color:#FF0040">'''G'''</span>T || 141 || Asn ⇒ Ser || N141S <br />
|-<br />
| CM880035 ||- ||C<span style="color:#FF0040">'''G'''</span>G ⇒ C<span style="color:#FF0040">'''A'''</span>G || 159 || Arg ⇒ Gln || R159Q <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=374591570 rs374591570] || 1062 || <span style="color:#FF0040">'''C'''</span>TC ⇒ <span style="color:#FF0040">'''T'''</span>TC || 213 || Leu ⇒ Phe || L213F <br />
|-<br />
| CM992894 ||- || G<span style="color:#FF0040">'''G'''</span>A ⇒ G<span style="color:#FF0040">'''A'''</span>A||241 || Gly ⇒ Glu || G241E <br />
|-<br />
| [http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=371083513 rs371083513] || 1470 || <span style="color:#FF0040">'''G'''</span>TA ⇒ <span style="color:#FF0040">'''A'''</span>TA ||349 || Val ⇒ Ile || V349I <br />
|-<br />
| CM960697 ||- || A<span style="color:#FF0040">'''C'''</span>G ⇒ A<span style="color:#FF0040">'''T'''</span>G || 408|| Thr ⇒ Met || T408M <br />
|-<br />
| CM880036 ||- || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C || 409 || Asn ⇒ Ser || N409S <br />
|- <br />
| CM870010 ||- || C<span style="color:#FF0040">'''T'''</span>G ⇒ C<span style="color:#FF0040">'''C'''</span>G || 483 || Leu ⇒ Pro || L483P <br />
|-<br />
| CM057072 ||- || A<span style="color:#FF0040">'''A'''</span>C ⇒ A<span style="color:#FF0040">'''G'''</span>C|| 501|| Asn ⇒ Ser || N501S <br />
|-<br />
|}<br />
<center><small>'''<caption>''' Information about 10 randomly selected mutations for glucocerebrosidase taken from HGMD (CM...) and dbSNP (rs...).</caption></small></center><br />
</figtable><br />
<br />
==Mutation Analysis==<br />
In our analysis we looked closer to the amino acid properties and their changing characteristics by mutation. We analyzed the structural difference between wild type (WT) and mutation. We also considered their secondary structure and distinguished between helix (H), sheet (E) and loop (C). We also took two different substitution matrices into account, BLOSUM62 and PAM250. '''P'''oint '''A'''ccepted '''M'''utation matrix has only positive integer values as scores and is not symmetric. The score reflects the probability of an amino acid to mutate into another. In contrast the '''BLO'''cks '''SU'''bstitution '''M'''atrix has also negative integers and is symmetric. A positive score indicates that a substitution occurs more than random. While a score of 0 shows that the substitution occurs randomly, a negative one points to a mutation less frequent than a random mutation. In case one of our selected mutations has the worst possible substitution score for this amino acid we highlighted the score red in <xr id="ana"/>.<br />
To consider also evolutionary information we created different PSSM matrices. These position specific scoring matrices are based on alignments. Just as BLOSUM, the PSSM has positive and negative integer values as scores. A positive value shows that the substitution occurs more often than expected. Critical functional residues, like active site residues, have high positive scores. One PSSM was created with a Psi-BLAST search. The other one is based on an alignment consisting of all mammalian homologous sequences.<br />
<br />
<br />
<figtable id="ana"><br />
{| border="1" cellpadding="13" cellspacing="0" align="center" <br />
|-<br />
! colspan="13" style="background:#adceff;" | Mutation Analysis<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Changes of Physiochemical Properties<br />
! colspan="2" style="background:#adceff;" | Structural Properties<br />
! colspan="7" style="background:#adceff;" | Conservation<br />
! colspan="1" style="background:#adceff;" | Effect<br />
|-<br />
! style="background:#efefef;" | Mutation<br />
! style="background:#efefef;" | From<br />
! style="background:#efefef;" | To<br />
! style="background:#efefef;" | Pymol Visualization<br />
! style="background:#efefef;" | Secondary Structure<br />
! style="background:#efefef;" | BLOSUM62 score<br />
! style="background:#efefef;" | PAM250 score<br />
! style="background:#efefef;" | PSSM score<br />
! style="background:#efefef;" | PSSM WT frequency<br />
! style="background:#efefef;" | PSSM mutatant frequency<br />
! style="background:#efefef;" | MSA WT frequency<br />
! style="background:#efefef;" | MSA mutant frequency<br />
! style="background:#efefef;" |<br />
|-<br />
| S77R ||polar, neutral charge, sulfur-containing || polar, positive charge, basic ||[[Image:mut_S38R.png|thumb|250x250px| Mutation of serine (blue) to arginine (orange) on position 77. ]] || E||-1 ||6||1||11%||9%|| 64%||2%|| slightly negative <br />
|-<br />
| N141S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing ||[[Image:mut_N102S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 141. ]] ||H ||1||5||0|| 10%||7%||55%||3%|| neutral<br />
|-<br />
| R159Q || polar, positive charge, basic || polar, neutral charge, acidic||[[Image:mut_R120Q.png|thumb|250x250px| Mutation of arginine (blue) to glutamine (orange) on position 159.]] || E|| 1||5||-4||83%||0%||86%|| 0%|| negative<br />
|-<br />
| L213F || nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, aromatic, hydrophobic||[[Image:mut_L174F.png|thumb|250x250px| Mutation of leucine (blue) to phenylalanine (orange) on position 213.]] || E||0||13||3||22% ||13%||100%||0%||slightly negative <br />
|-<br />
| G241E || nonpolar, neutral charge, aliphatic || polar, negative charge, acidic||[[Image:mut_G202E.png|thumb|250x250px| Mutation of glycine (blue) to glutamic acid (orange) on position 241.]]||C || -2 ||9||-1|| 10%||3%||83%||0%||slightly negative <br />
|-<br />
| V349I ||nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, aliphatic, hydrophobic ||[[Image:mut_V310I.png|thumb|250x250px| Mutation of valine (blue) to isoleucine (orange) on position 349. ]] ||E ||3 || <span style="color:#FF0040">4</span>||0||14%|| 5%||97%||3%||neutral<br />
|-<br />
| T408M || polar, neutral charge, hydroxyl-containing || nonpolar, neutral charge, sulfur-containing||[[Image:mut_T369M.png|thumb|250x250px| Mutation of threonine (blue) to methionine (orange) on position 408. ]] || H|| -1 ||5||-1|| 4%||2%||82%||0%||neutral<br />
|-<br />
| N409S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing|| [[Image:mut_N370S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 409.]] ||H ||1||5||1|| 10%||9%||76%||2%||slightly negative <br />
|- <br />
| L483P || nonpolar, neutral charge, aliphatic, hydrophobic || nonpolar, neutral charge, cyclic||[[Image:mut_L444P.png|thumb|250x250px| Mutation of serine (blue) to arginine (orange) on position 483]] ||E ||-3||5||-3|| 29%||1%||100%||0%|| high negative<br />
|-<br />
| N501S || polar, neutral charge, acidic || polar, neutral charge, sulfur-containing||[[Image:mut_N462S.png|thumb|250x250px| Mutation of asparagine (blue) to serine (orange) on position 501.]] ||E ||1||5||-2||87%||3%||86%||1%|| negative<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Analysis of the chosen mutations of <xr id="sele"/> in the field of their properties, secondary structure and conservation. The secondary structure can be classified as helix (H), sheet (E) and loop (C). In case a mutations is the worst possible substitution for this amino acid, the substitution matrix score is colored red. The effect of the mutation is based on our analysis. A detailed description can be read below.</caption></small></center><br />
</figtable><br />
<br />
Based on the analysis summed up in <xr id="ana"/> we interpreted our mutations:<br />
<br />
'''S77R : ''' The biggest change happens in the secondary structure. While serine has a short and neutral side chain, arginine shows a much longer positive side chain, that probably causes a clash with the flexible loops of the environment. Additional to the change in its polarity the residue switches from sulfur containing to basic. This could destabilize its secondary structure, as the parallel located sheet may be not fixed anymore to the sheet of the residue. The PSSM show only no high frequency for the WT as well as the mutant. With scores of -1 and 6, the substitution matrices identifies the point mutation as expectable. We think the only effect comes from the structural change and has a slightly negative effect.<br />
<br />
'''N141S : '''<br />
The mutation causes no change in its charge and polarity. The affected residue is located in a helix on the protein surface, which let us assume that no effect may occur (neutral). The substitution matrices as well as the PSSM score confirm us to this opinion, as the scores (1[BLOSUM], 5[PAM] and 0[PSSM]) indicate the mutation as nearly random. Also the PSSM frequency tells us that the mutation is rare (7% and 3%) and the WT not very distinct (10% and 55%).<br />
<br />
'''R159Q : '''<br />
The substitution changes the residue from basic and positive charged into a acidic residue without charge. The mutant has a much longer side chain which extends deep into the protein. This structural characteristics as well as the great pH change let us assume that a clash or effect on the structure around this amino acid cannot be avoided. The scores, especially the evolutionary based position specific score, affirms us in our assumption. In the end, the frequency of the WT (83% and 86%) as well as the absence of the mutant in the MSAs, leave us no doubt of the mutation severeness. <br />
<br />
'''L213F : '''<br />
The amino acid is located in a sheet and turns from aliphatic to aromatic. Although, this is a great structural change, there seem to be no clashes or other influences on the neighborhood. Both substitution matrices indicate this to occur on random. Even though, the PSSM shows an mutation appearance more than random and the mutation can be seen in 13% of the alignment sequences, the MSA of homologous sequences consists only of leucine at this position. However, we think that the mutation may be neutral, but uncommon between the homologous sequences. As we are not quite sure, we defined it as possible damaging.<br />
<br />
'''G241E : '''<br />
There is great change in the physicochemical properties caused by the mutation from glycine to glutamine acid, especially for the charge and pH. The residue appears in a loop on the protein surface. Its side chain does not extend into the protein. That is why the property change will not have an effect on the protein structure. The substitution scores deviate from each other. While BLOSUM shows an occurrence less than random, PAM indicates this mutation to happen more frequent. Also the different MSAs show frequencies that make the data interpretation difficult. Due to the combination of the structural and the property characteristics, we would assume the mutation as neutral. But because of the remaining data, we are not sure and declare it as slightly damaging. <br />
<br />
'''V349I : '''<br />
The mutation from valine to isoleucine makes no difference in the properties. Also, both branch chain amino acids differ only slightly in their structure. The PAM declares the substitution as rare, as the score of 4 is the worst for valine, but occurs for several amino acids. Based on evolutionary information the PSSM score defines the mutation as random. This is not surprising concerning their similarity. In the MSAs both amino acids appear at position 349, but valine more often. Based on this observation, we are convinced that this is a neutral mutation between similar amino acids, which occurs once in a while.<br />
<br />
'''T408M : '''<br />
The mutant and the WT differ in their polarity. While threonine is hydroxyl containing, methionine has a sulfur atom in its structure. The side chain causes no clashes with other residues. This substitution happens little less than random. Both amino acids are rare at position 408 in the PSSM alignment. Considering only homologous sequences the WT occurs way more often (82%). The mutant is not observable. We interpret this data as a neutral mutation.<br />
<br />
'''N409S : '''<br />
The mutation causes no difference in charge and polarity. It occurs in a helix and lies close to a sheet, which lets us assume that they stabilize each other. However, the mutation may not destabilize the structure very much. The substitution happens nearly random. In the PSSM MSA, the mutant appears nearly as often as the WT (difference 1%). But the frequencies in the homologous alignment differ much more (difference 73%). We think that this mutation has a minimal effect on the protein that can cause a slight form of the disease, but definitely not deadly. <br />
<br />
'''L483P : '''<br />
There seem to be no great changes in the pysicochemical properties except the cyclic characteristic of proline. The residue is located at the end of a sheet and may be not important for the structure stabilization, but it is obvious that the ring clashes with following residues of the adjoining loop which can be severe for the structure. Compared to lysine, proline has one amino group less which can also cause to a destabilization. Especially the BLOSUM and PSSM scores (both -3) show an rare occurrence of the mutation. The frequency balance between mutation and wild type is very dissimilar (29%>>1%). By looking at the evolutionary information of the homologs, we can see that the WT is present in all sequences. This can give as a hint to the severeness of the mutation. The reason for never showing up as well as a rare appearance, can be that the mutation causes not only a disease but death. All information about this mutation let us identify a high negative effect. <br />
<br />
'''N501S : '''<br />
The pysicochemical properties have been described before for mutation N409. The amino acid is located at the beginning of a beta sheet. The side chain does not clash with residues in its neighborhood. The substitution matrix scores show that the mutation appears more than randomly. But the PSSM score shows a lower probability for the mutation. Also the alignments show that the WT occurs much more often (87% and 86%) than the mutant. This frequency tells us that the mutation happens seldom. We suppose this as an observation of selection pressure. So, we assume that the mutation is disease causing.<br />
<br />
==Comparison of different approaches==<br />
First of all we interpreted our collected data from analysis of <xr id="ana"/>. After that we run several predictors of mutation effects. All these results are summarized in <xr id="app"/>. Then we reassessed our analysis by considering the prediction results. In the end we validated our new interpretation (consensus in <xr id="app"/>) against the databases dbSNP and HGMD. In two cases, both databases had contrary mutation information. While HGMD identifies them as disease causing, dbSNP classifies the two mutations as non disease causing. That is why we marked them as possibly damaging.<br />
For three mutations (N141S, R159Q, N501S) all predictions, our interpretations, and the validation totally agree with each other. So, we can say for sure that the mutation of asparagine to serine on position 141 has no effect. However, the mutation on position 159 from arginine to glutamine is defintely disease causing as well as the SNP at position 501 from asparagine to serine. <br />
In the end, we classified the mutation N409S as disease causing, because the score of SIFT is marginal to disease causing, which would answer our assumption of a slight disease. <br />
<br />
<figtable id="app"><br />
{| border="1" cellpadding="8" cellspacing="0" align="center" <br />
|-<br />
! colspan="8" style="background:#adceff;" | Summary of different prediction approaches<br />
|-<br />
! style="background:#efefef;" | Mutation<br />
! style="background:#efefef;" | Analysis of <xr id="ana"/><br />
! style="background:#efefef;" | SIFT <br />
! style="background:#efefef;" | Polyphen2 (HumDiv)<br />
! style="background:#efefef;" | MutationTaster<br />
! style="background:#efefef;" | SNAP<br />
! style="background:#efefef;" | Consensus<br />
! style="background:#efefef;" | Validation<br />
|-<br />
|S77R <br />
! style="background:#F4FA58;" | pcd<br />
! style="background:#2EFE64;" | 0.37 <br />
! style="background:#2EFE64;" | 0.17<br />
! style="background:#FA5858;" | 90%<br />
! style="background:#2EFE64;" | 2<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|N141S<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | 0.15<br />
! style="background:#2EFE64;" | 0.01<br />
! style="background:#2EFE64;" | 83%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|R159Q<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 1<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#FA5858;" | 7<br />
! style="background:#FA5858;" | dc <br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|L213F<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.79<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#F4FA58;" | HGMD[dc] vs dbSNP[ndc]<br />
|-<br />
|G241E<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#FA5858;" | 0.01 <br />
! style="background:#FA5858;" | 0.89<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#F4FA58;" | HGMD[dc] vs dbSNP[ndc]<br />
|-<br />
|V349I<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | 0.25 <br />
! style="background:#2EFE64;" | 0.12<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#2EFE64;" | dbSNP<br />
|-<br />
|T408M<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#FA5858;" | 0.03 <br />
! style="background:#2EFE64;" | 0.11<br />
! style="background:#2EFE64;" | 60%<br />
! style="background:#2EFE64;" | 5<br />
! style="background:#2EFE64;" | ndc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|N409S<br />
! style="background:#F4FA58;" | pdc<br />
! style="background:#2EFE64;" | 0.05 <br />
! style="background:#2EFE64;" | 0.23<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|L483P<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.85<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#2EFE64;" | 1<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|N501S<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | 0 <br />
! style="background:#FA5858;" | 0.98<br />
! style="background:#FA5858;" | 100%<br />
! style="background:#FA5858;" | 0<br />
! style="background:#FA5858;" | dc<br />
! style="background:#FA5858;" | HGMD<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Information about selected mutations from different predictors of amino acid substitution effects as well as our own interpretation based on our data of the previous exercises of the task 08. The consensus is our opinion of the effect based on the predictions and our analysis. We distinguish between three different kind of effects: <span style="background:#FA5858">'''disease causing (dc)'''</span>, <span style="background:#F4FA58">'''possibly damaging (pdc)'''</span> and <span style="background:#2EFE64">'''non-disease causing (ndc)'''</span>. The prediction scores are colored according to this. The validation contains the information of the databases (HGMD, dbSNP).</caption></small></center><br />
</figtable></div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_02_-_Alignments&diff=38906
Gaucher Disease: Task 02 - Alignments
2013-09-06T00:39:04Z
<p>Kalemanovm: </p>
<hr />
<div>Alignments allow comparisons of strings. In the field of bioinformatics, sequence alignments provide a measure of relatedness between two or more sequences, which is the basis of many different predictions. <br />
==Theoretical Background==<br />
<br />
===Description===<br />
Pairwise or multiple alignments often contain an additional line below the proper alignment or between two aligned sequences. This line gives a more accurate description of the relation of the aligned residues. The symbols show if there is match between identical amino acids or if they are only similar.<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Symbols for describing sequence alignments<br />
|-<br />
! style="background:#efefef;" align="center" | Program(s)<br />
! style="background:#efefef;" align="center" | Symbol<br />
! style="background:#efefef;" align="center" | Example<br />
! style="background:#efefef;" align="center" | Meaning<br />
|-<br />
| align="center" rowspan="3" | MSAs<br />
| align="center" |*<br />
| align="center" |A<br>A<br>*<br />
| align="center" |identical residues<br />
|-<br />
| align="center" |:<br />
| align="center" |I<br>L<br>:<br />
| align="center" |similar residues<br />
|-<br />
| align="center" |.<br />
| align="center" |S<br>P<br>.<br />
| align="center" |similar residues<br />
|-<br />
| align="center" rowspan="2" | (Psi-)BLAST<br />
| align="center" |same letter <br />
| align="center" |A<br>A<br>A<br />
| align="center" |identical residues<br />
|-<br />
| align="center" |+<br />
| align="center" |L<br>+<br>V<br />
| align="center" |similar residues<br />
|-<br />
| align="center" rowspan="3" | HHblits<br />
| align="center" |<nowiki>|</nowiki><br />
| align="center" |AF<br><nowiki>| |</nowiki><br>AW<br />
| align="center" |identical or very similar residues<br />
|-<br />
| align="center" |+<br />
| align="center" |T<br>+<br>S<br />
| align="center" |similar residues<br />
|-<br />
| align="center" |.<br />
| align="center" |N<br>.<br>H<br />
| align="center" |non-similar residues<br />
|-<br />
|}<br />
<br />
==Sequence Searches==<br />
<br />
===Runs===<br />
Sequence searches with our query protein sequence - enzyme glucocerebrosidase, P04062.fasta, were done with the following programs:<br />
<br />
* '''BLAST''' <br />
** using standard parameters<br />
** against big_80<br />
** against big<br />
<br />
* '''Psi-BLAST''' <br />
** with number of shown hits and alignments set to 10000 (-b, -v options), so that all the hits will be shown. <br />
** with all combinations of:<br />
*** 2 iterations: 1 iterations against big_80 followed by 1 iteration against big<br />
*** 10 iterations: 9 iterations against big_80 followed by 1 iteration against big<br />
*** default E-value cutoff (0.002)<br />
*** E-value cutoff 10E-10<br />
** other options leaved default<br />
<br />
* '''HHblits'''<br />
** with number of shown hits and alignments set to 10000 (-Z, -B options), as in Psi-BLAST <br />
** with all combinations of:<br />
*** 2 iterations against uniprot_20<br />
*** 10 iterations against uniprot_20<br />
*** default E-value cutoff (0.001)<br />
*** E-value cutoff 10E-10<br />
** other options leaved default<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
HHblits searches among representative sequences from different uniprot_20 clusters. When a representative from one cluster is found, all the sequences from this cluster are being added to the hit list. Thus HHblits finds much more hits than PSI-BLAST. Therefore, one additional PSI-BLAST run against the unfiltered big database was performed, using checkpoint files of the last profile created from the previous search in big80, in order to find as much related sequences as possible, as HHblits does indirectly. Summing up, the following databases were used: big_80 for profile building and big for the last iteration for PSI-BLAST and uniprot20 for HHblits. BLAST runs (consisting only from one iteration), we searched once against big_80 and once against big to see the difference in number of found hits.<br />
<br />
===Comparison===<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
====Number and overlap of hits====<br />
<br />
Number of found hits with each program and parameter combination and overlap of hits between some are summarized in <xr id="hit"/>.<br />
<br />
<figtable id="hit"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Number and overlap of hits<br />
|-<br />
! style="background:#efefef;" align="center" | Program<br />
! style="background:#efefef;" align="center" | Settings<br />
! style="background:#efefef;" align="center" | Number of hits<br />
! style="background:#efefef;" align="center" | Of them overlapped - with ...<br />
|-<br />
|align="center" rowspan="2" | BLAST<br />
|big_80<br />
|569<br />
|310 - both HHblits searches with E-val 0.001<br />
|-<br />
|big<br />
|1209<br />
|643 - both HHblits searches with E-val 0.001<br />
|-<br />
|align="center" rowspan="9" | Psi-BLAST<br />
|all combinations with 1 iter big<br />
|1216<br />
|644 - HHblits search with same combination<br />
|-<br />
|1 iter big_80 + 2 iter big, default E-val<br />
|2274<br />
|1126 - HHblits 2 iter, default E-val<br />
|-<br />
|1 iter big_80 + 2 iter big, E-val 10E-10<br />
|2099<br />
|1071 - HHblits 2 iter, E-val 10E-10<br />
|-<br />
|9 iter big_80 + 2 iter big, default E-val<br />
|2274<br />
|1174 - HHblits 10 iter, default E-val<br />
|-<br />
|9 iter big_80 + 2 iter big, E-val 10E-10<br />
|2099<br />
|1069 - HHblits 10 iter, E-val 10E-10<br />
|-<br />
|2 iter big_80, default E-val<br />
|1092<br />
|<br />
|-<br />
|2 iter big_80, E-val 10E-10<br />
|987<br />
|<br />
|-<br />
|10 iter big_80, default E-val<br />
|4031<br />
|<br />
|-<br />
|10 iter big_80, E-val 10E-10<br />
|1525<br />
|<br />
|-<br />
|align="center" rowspan="4" | HHblits<br />
|2 iter., E-val 0.001<br />
|1985<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|2 iter., E-val 10E-10<br />
|1922<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|10 iter., E-val 0.001<br />
|17538<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|10 iter., E-val 10E-10<br />
|3189<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Number and overlap of hits from Blast, Psi-BLAST and HHblits.</caption></small></center><br />
</figtable><br />
<br />
'''Observations'''<br />
*BLAST search against not filtered big yields significantly more hits than against the redundancy reduced big_80.<br />
*Iterative BLAST (i.e. Psi-BLAST) finds far more hits than BLAST.<br />
*HHblits finds more hits than BLAST and Psi-BLAST (latter when number of iterations comparable).<br />
*HHblits finds more hits after 10 iterations than after 2 iterations.<br />
*HHblits finds less hits when lower E-value cutoff (10E-10) is used. For 2 iterations it is a little less but for 10 iterations it is much less.<br />
*The "high outlier" is number of hits with HHblits search with 10 iterations and default E-value inclusion cutoff.<br />
*Psi-BLAST number of hits is the same in all 4 parameter combinations after 1 last iteration against big. Thought after another iteration against big (done because of the check files), the number of hits using the default E-value cutoff is higher than using the E-value cutoff 10E-10. However, the number of hits stays the same independent of the number of "profile building" iterations against big_80 (1 or 9). In the second iteration against big much more hits are found, almost the double. -> Is one iteration against big not enough to "unfold" the profile?<br />
*Psi-BLAST after the 4 different runs only against big_80 are summarized in the table. The trends of the number of hits are comparable to HHblits (more hits with more iterations and less hits with the more restrictive E-value).<br />
*Number of hits found by both HHblits and Psi-BLAST or both HHblits and BLAST is almost the same and independent of the search parameters we used (therefore in Psi-BLAST the same hits are found after 1 iteration against big with all parameter combinations). But the overlap is significantly higher after 1 additional Psi-BLAST iteration against big.<br />
<br />
To gain more understanding about the found hits using different programs and search options, E-value, percentage identity and alignment length distributions were plottet.<br />
<br />
====E-value distribution====<br />
<figure id="evalue"><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' E-value distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST has a similar E-value distribution as Psi-BLAST.<br />
*Same range of E-value for (Psi-)BLAST and HHblits, however, different frequency of different values: (Psi-)BLAST has more higher E-values (left side) while HHblits has two peaks at high and low values.<br />
*For a high number of iterations, HHblits finds more hits with lower E-values (especially with default E-value cutoff).<br />
<br />
====Percentage identity distribution====<br />
<figure id="identity" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_identity_hist.png|Identity distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_identity_hist.png|Identity distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_identity_hist.png|Identity distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_identity_hist.png|Identity distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' Identity distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST and Psi-BLAST have similar identity distributions, with peaks at about 30%, 85% and 100%.<br />
*HHblits has another high peak at about 20%, which moves to even lower identities with higher number of iterations. This peak is especially high with 100 iterations and default E-value cutoff, because of the high number of found hits.<br />
<br />
====Alignment length distribution====<br />
<figure id="length" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' Alignment length distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST and Psi-BLAST have similar alignment length distributions.<br />
*HHblits and (Psi-)BLAST alignment lengths have similar range from 1 to round 550 residues.<br />
* In general, (Psi-)BLAST seems to produce longer alignments than HHblits.<br />
<br />
===Evaluation===<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
====Results====<br />
<br />
Resulting plots of TPR and precision as a function of E-value are shown in <xr id="blast_results"/>, <xr id="psiblast_results"/> and <xr id="hhblits_results"/>.<br />
<br />
'''BLAST'''<br />
<figure id="blast_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:blast-big_80_eval_tpr_precision.png|TPR and precision vs. E-value of BLAST hits after 1 big_80 iteration with default parameters using the L30, L40 and L60 COPS groups as gold standard. On the X-axis E-value is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:blast-big_eval_tpr_precision.png|TPR and precision vs. E-value of BLAST hits after 1 big iteration with default parameters using the L30, L40 and L60 COPS groups as gold standard. On the X-axis E-value is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of BLAST hits searched in big_80 and big database.</caption></small><br />
</figure><br />
<br />
'''Psi-BLAST'''<br />
<figure id="psiblast_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:psiblast-1iter-big_80--1iter_big--1iter_pdb_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 1 big_80, 1 big and 1 pdb_full iterations with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-1iter-big_80--1iter_big--1iter_pdb-10E-10_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 1 big_80, 1 big and 1 pdb_full iterations with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-9iter-big_80--1iter_big--1iter_pdb_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 9 big_80, 1 big and 1 pdb_full iterations with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-9iter-big_80--1iter_big--1iter_pdb-10E-10_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 9 big_80, 1 big and 1 pdb_full iterations with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''HHblits'''<br />
<figure id="hhblits_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20--1iter-pdb.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 2 uniprot_20 iterations and 1 pdb_full iteration with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-2iter-uniprot20--1iter-pdb-10E-10.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 2 uniprot_20 iterations and 1 pdb_full iteration with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-10iter-uniprot20--1iter-pdb.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 10 uniprot_20 iterations and 1 pdb_full iteration with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-10iter-uniprot20--1iter-pdb-10E-10.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 10 uniprot_20 iterations and 1 pdb_full iteration with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of HHblits hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*In '''BLAST''' search against '''big_80''', only one hit was found. It has the same L60 (therefore also L40 and L30) group as the query. It can be seen on the plot as a pair of points with the highest precision and lowest TPR.<br />
*In '''BLAST''' search against '''big''', only hits with two different E-values were found. Also there the precision is high but TPR low, especially for hits with same L30 group.<br />
*For the other programs - '''HHblits''' and '''Psi-BLAST''' - it can be observed, that TPR rises with growing E-value and precision drops with growing E-value, because then more TP and FP hits are found.<br />
**TPR rises with growing E-value, because more TP hits are detected, especially rapidly for L60. This indicates that more similar hits are detected more easily, almost all L60 are found. However only a small subset of L40 and L30 hits are retrieved. The TPR plot is similar for Psi-BLAST and HHblits searches. '''HHblits''' achieves slightly higher precision then '''Psi-BLAST''': 24% vs. 9% for L30, 40% vs. 15% for L40. Both programs find all L60 hits.<br />
**There is a different behavior in precision:<br />
***In '''Psi-BLAST''' searches, precision stays 100% until approx. E-value 10E-20, then it starts to drop, more rapidly for L60 group than for L40 and L30 groups. It shows that hits with high E-values probably are not very similar to the query. <br />
***In '''HHblits''' searches, precision is 100% for very low E-values, then as E-value grows precision of all structural groups falls rapidly to about 90% and stays there for a while. After some E-value is achieved (usually approx. > 10E-10), precision continues to drop further. Also here precision for L60 groups falls more rapidly than for L40 and L30 groups.<br />
** '''Psi-BLAST''' results for different settings are overall very similar, except that less TP are found with E-value inclusion cutoff 10E-10.<br />
**For '''HHblits''' there is one exeption: the plot for HHblits with 10 iterations and default E-value cutoff (for inclusion into profile) looks very different. The highest TPR values are achieved: 30% for L30, 52% for L40 and 100% for L60, however the precision drops not only already at very low E-values to about 90%, as also for other HHblits searches, but it continues to drop already after E-value 10E-30 for all the structural groups (again more rapidly for L60). This indicates that many HHblits iterations combined with not very restrictive E-value inclusion cutoff 0.001 allow too many FP to penetrate into the profile and in the end into the result list. We do not recommend to use these search settings.<br />
**It could be a good idea to remove '''Psi-BLAST''' hits with E-value > 10E-20 and HHblits hits with E-value > 10E-10 (in most of the cases) to reduce the number of FP. However then we will loose some TP that are still found with these high E-values (L30 and L40).<br />
<br />
==Multiple Sequence Alignment==<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
For the multiple sequence alignments, three sets were created. Therefore, the results of the previous task were used according to their sequence identity to the native protein sequence of glucocerebrosidase. Set 1 and set 2 contain 10 sequences each, including the native protein sequence to keep the alignments in relation to the Gaucher disease causing protein. The remaining 9 sequences have a sequence identity to the glucocerebrosidase of more than 60% in set 1 and less than 30% in set 2. In set 3, the 20 sequences are over the whole range of sequence identity. <br />
<br />
In the following analyses the term "conserved region" is used for a section of columns that feature a higher number of conserved columns or columns of similar residues. Moreover, the term "contiguous gap" is used for an amount of consecutive gaps that split up the protein sequence.<br />
<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="left"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Set 1: sequence identity >60%<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|-<br />
|A9UD35<br />
|84.1<br />
|-<br />
|D1L2S0<br />
|83.0<br />
|-<br />
|3gxi_A<br />
|99.8<br />
|-<br />
|2nt1_A<br />
|99.8<br />
|-<br />
|F6WDY8<br />
|90.7<br />
|-<br />
|Q2KHZ8<br />
|89.2<br />
|-<br />
|F5CB27<br />
|81.8<br />
|-<br />
|F5H241<br />
|98.2<br />
|-<br />
|B7Z6S9<br />
|99.8<br />
|-<br />
|}<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="align"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Set 2: sequence identity <30%<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|-<br />
|H6CEV7<br />
|26.5<br />
|-<br />
|Q21GD0<br />
|24.3<br />
|-<br />
|I1WBF3<br />
|23.7<br />
|-<br />
|I9HH59<br />
|29.4<br />
|-<br />
|D0TN48<br />
|25.4<br />
|-<br />
|B1VPJ0<br />
|27.5<br />
|-<br />
|E2LY19<br />
|24.8<br />
|-<br />
|K9HBW2<br />
|25.9<br />
|-<br />
|B5QQZ8<br />
|27.1<br />
|-<br />
|}<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="align"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Set 3: sequence identity over all<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|B5DYA3<br />
|32.0<br />
|-<br />
|B4JTN5<br />
|34.1<br />
|F5CB37<br />
|81.8<br />
|-<br />
|E1ZYU8<br />
|42.7<br />
|J2STU0<br />
|34.2<br />
|-<br />
|F4E6W5<br />
|26.4<br />
|G9BHQ3<br />
|80.7<br />
|-<br />
|F4X220<br />
|41.7<br />
|2nsx_B<br />
|99.8<br />
|-<br />
|E5UPZ0<br />
|21.6<br />
|G9BHQ5<br />
|82.5<br />
|-<br />
|C6A5Q0<br />
|25.0<br />
|D0TIX6<br />
|25.8<br />
|-<br />
|B1QKT0<br />
|27.8<br />
|1y7v_A<br />
|99.8<br />
|-<br />
|D4SV88<br />
|34.6<br />
|A9UD58<br />
|80.4<br />
|-<br />
|A9UD54<br />
|81.8<br />
|G9MUP6<br />
|20.7<br />
|-<br />
|}<br />
<br />
===ClustalW===<br />
<br />
Multiple alignments generated with ClustalW:<br />
<figure id="cl_1" ><br />
[[Image:fullrange_set1.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with ClustalW</caption>]]<br />
</figure><br />
<figure id="cl_2" ><br />
[[Image:set2_clustalw.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with ClustalW</caption>]]<br />
</figure><br />
<figure id="cl_3" ><br />
[[Image:set3_clustalw.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with ClustalW</caption>]]<br />
</figure><br />
<br />
<br />
'''Set 1''', which includes sequences with high similarity, has only 11 conserved columns. But these columns lie densely in an alignment area between position 284 and 337 (marked darkblue in <xr id="cl_1"/>). Also columns that are not conserved by identical residues, but have similar amino acids, can be found in this conserved region. This conserved area forms two alpha helices and one beta-sheet in its secondary structure. The gaps of the alignment split the protein into 5, parts of residues. Especially the last 400 residues have a very high sequence identity between the glucosylcerebrosidase and 6 other proteins. The last contiguous gap which is also the longest one lies inside an alpha helix of the secondary structure. <br />
<br />
'''Set 2''' has less conserved columns than set 1 (<xr id="cl_2"/>). They are spread over the alignment and build no conserved region. The contiguous gaps are more but shorter.<br />
<br />
There exist no conserved columns for '''set 3''' (<xr id="cl_3"/>). This could result from the greater number of sequences in the set. The more sequences are in an alignment the lesser the probability of having conserved columns. Furthermore, also the sequences with a low sequence identity cause to a loss of a conserved region. <br />
<br />
<br />
<figtable id="clu"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of ClustalW in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|11<br />
|8<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|3797<br />
|5239<br />
|6721<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|6<br />
|17<br />
|16<br />
|-<br />
|Alignment length<br />
|762<br />
|1079<br />
|722<br />
|}<br />
<center><small>'''<caption>''' ClustalW statistics of glucocerebrosidase.</caption></small></center><br />
</figtable><br />
<br />
===Muscle===<br />
Multiple alignments generated with Muscle:<br />
<figure id="m1" ><br />
[[Image:set1_muscle.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with Muscle</caption>]]<br />
</figure><br />
<figure id="m2" ><br />
[[Image:set2_muscle.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with Muscle</caption>]]<br />
</figure><br />
<figure id="m3" ><br />
[[Image:set3_muscle.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with Muscle</caption>]]<br />
</figure><br />
<br />
<br />
In '''set 1''' the native protein sequence itself has no gaps. There are only gaps at the beginning of the sequence, because the alignment (<xr id="m1"/>) is longer than the length of the glucocerebrosidase sequence. There are 10 conserved columns which all concentrate on the alignment positions between 138-204. The remaining residues of these area also have a high similarity between the different sequences. Only the sequence F5H241 is splitted by a few gaps at its beginning. This high conserved region affects a different region of the glucocerebrosidase (residues 53-119) than the high conservative region detected by ClustalW (residues 171-220).<br />
<br />
'''Set 2''' has also 10 conserved columns (<xr id="m2"/>). But in contrast to set 1, they are widely spread over the alignment. The same observation can be made about columns with similar residues. Through this scattering it seems rather a randomly generated conserved column than a conservation due to functional reasons. The high number as well as the partly great length of the contiguous gaps straighten the alignment. The alignment gives a great overview of the relation between sequences with low similarity and sequence identity. It also shows that for finding functional important areas that are conserved, a higher similarity and identity is needed.<br />
<br />
'''Set 3''' has no conserved columns (<xr id="m3"/>), for the same reason as explained before, for set 2. It has indeed the highest number of gaps of the three sets. But contrary to the alignment of set 2 the contiguous gaps are shorter, partially they are only 1-2 gaps long. So the alignment appears less straightened, as the sequences with high sequence identity keep the alignment a bit compact. <br />
<br />
<br />
<figtable id="mus"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Muscle in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|10<br />
|10<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|5609<br />
|7341<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|26<br />
|29<br />
|-<br />
|Alignment length <br />
|621<br />
|1116<br />
|753<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Muscle statistics of glucocerebrosidase. </caption></small></center><br />
</figtable><br />
<br />
===T-Coffee===<br />
Multiple alignments generated with T-Coffee:<br />
<figure id="tc1" ><br />
[[Image:set1_coffe.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with T-Coffee</caption>]]<br />
</figure><br />
<figure id="tc2" ><br />
[[Image:set2_coffe.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with T-Coffee</caption>]]<br />
</figure><br />
<figure id="tc3" ><br />
[[Image:set3_coffe.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with T-Coffee</caption>]]<br />
</figure><br />
<br />
<br />
The Alignment of '''set 1''' in <xr id="tc1"/> generated by T-Coffee is very similar to its alignment made by Muscle. However, there exist no conserved columns. This is caused because of the shift of the sequences. So, in no alignment position, the residues of all sequences are aligned. The high conserved region of the Muscle-alignment is almost identical to a conserved region in the T-coffee generated alignment. They only differ in a shift of a part of sequence F5H241. The First few residues are now aligned at the beginning of the glucocerebrosidase instead of position 53. While Muscle includes gaps in this F5H241 part, T-Coffe does not separate the shifted residues.<br />
<br />
The other difference to the Muscle alignment is one long contiguous gap inside of the protein sequence A9UD35. This extremely long contiguous gap shifts the last amino acid of the sequence to the last position of the alignment. As this amino acid (G) is not identical to the remaining amino acids (Q) at the last alignment position of the other sequences, there seem to be no reason for this observation. The suspicion of an error arises, as the amino acid (G) would perfectly fit directly at the end of the sequence without a contiguous gap. <br />
<br />
'''Set 2''' shows widely spread conserved columns without building a conserved region. The alignment (<xr id="tc2"/>) itself has lots of gaps. An interpretation of the alignment in a biological way should be made carefully, as it bases on low sequence identity and similarity. Noticeable for this alignment is its beginning and end. All sequences are aligned in these areas with at least 4 residues. <br />
<br />
Sequences with an identity over the full range ('''set 3''') generate many gaps of different length and no conserved columns (<xr id="tc3"/>). Two different pattern are observable. On the one hand blocks of aligned residues are separated mostly by a block of gaps and are only connected by 1-2 sequences (for example positions 495-515). On the other hand a block is not completely aligned by residues of all sequences, but has an contiguous gap of a few sequences (for example positions 415-450). <br />
<br />
<br />
<figtable id="cof"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Muscle in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|0<br />
|12<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|6719<br />
|9361<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|44<br />
|50<br />
|-<br />
|Alignment length <br />
|621<br />
|1227<br />
|854<br />
|-<br />
|}<br />
<center><small>'''<caption>''' T-Coffee statistics of glucocerebrosidase.</caption></small></center><br />
</figtable><br />
<br />
===Espresso===<br />
Espresso is an advanced program of T-Coffee. While T-Coffee only uses sequences, Espresso also takes the 3D structure of the proteins into account.<br />
<br />
Multiple alignments generated with Espresso:<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/80555308/result.score_html Set1] (>60% seq.identity to glucocerbrosidase)<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/ccc1fd04/result.score_html Set2] (<30% seq.identity to glucocerbrosidase)<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/4903ac99/result.score_html Set3] (all % seq.identity to glucocerbrosidase)<br />
(Please note: in case of an unavailability of the alignments in the links above, please contact the author)<br />
<br />
The additional use of the structure improves the alignment of sequences with high sequence identity ('''set 1'''). In contrary to T-Coffee, Espresso detects now conserved columns. Through a little difference in the alignment score calculating, the residues in the beginning of the F5H241 aligne different than in T-Coffee. Now an conserved area between residues 53 and 119, including 12 high conserved columns and 9 columns with similar amino acids, can be seen in the alignment. The residues are now aligned not equal but similar and better to those in the Muscle-alignment. These conserved area forms four beta-sheets in its secondary structure.<br />
Also the last residue of A9UD35 which was mentioned before in connection with T-Coffee is now aligned directly at the end of its sequence.<br />
<br />
The two other sets containing also sequences with low sequence identity show no remarkable improvement to the results of T-Coffee .<br />
<br />
<br />
<figtable id="es"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Espresso in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|12<br />
|11<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|6769<br />
|9341<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|46<br />
|51<br />
|-<br />
|Alignment length <br />
|621<br />
|1232<br />
|853<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Espresso statistics of glucocerebrosidase</caption></small></center><br />
</figtable><br />
<br />
===Note===<br />
The generated T-Coffee and Espresso alignments of set 2 and 3 seem to focus aligned residues at their beginning or their end. So the question arises: Why? Why are these residues aligned, even though enormous gap penalty occurs considering the resulting long contiguous gaps. One thing is certain: T-Coffee does not use a freeshift alignments for generating a multiple alignment.<br />
<br />
===Alignment Comparison===<br />
Alignment comparison of the MSA of sequences with high similarity ('''Set 1''') generated by the programs described above.<br />
<br />
The Alignments produced by Muscle, T-Coffee and Espresso are very similar. They only differ in their aligning of the sequence F5H241. In case of Espresso the sequence was aligned so that a conserved region is observable. The conserved region, detected by ClustalW is on a different position. <br />
<br />
Nevertheless the resulting trees in <xr id="sid"/> based on the Average % of sequence identity differ from each other.<br />
<br />
<figure id="sid" ><br />
<gallery widths=285px heights=200px caption=""><br />
File:clustal_seqid.png|'''ClustalW'''<br />
File:muscle_seqid.png |'''Muscle'''<br />
File:coffe_seqid.png |'''T-Coffee'''<br />
</gallery><br />
<small>'''<caption>''' Trees of sequences with high similarity (set1) paired by average distance using %Identity. </caption></small><br />
</figure><br />
<br />
<br />
The trees (<xr id="sid"/>) based on the alignment generated by ClustalW and T-Coffee are identically in their sequence pairing. All human proteins can be found in one subtree. The same proteins are paired in both trees, just as the remaining mammalian proteins do in the other subtree. They only differ in their distances between paired sequences. This is an unexpected observation, as both alignments differ especially in their conserved region. <br />
But the tree showing the alignment from Muscle deviates enormously from the other two trees. Indeed, the mammalian subtree coincide with the others, but the human proteins do not. The human protein F5H241 which is directly paired to the glucocerebrosidase by ClustalW and T-Coffee is now linked (yellow) to the subtree consisting of all proteins (red, blue). This is the protein which is aligned different for Muscle and T-Coffee. So Muscle shows a different %Identity between the aligned sequences than ClustalW or T-Coffee.<br />
<br />
<br />
<figure id="ad" ><br />
<gallery widths=285px heights=200px caption=""><br />
File:clustal_bl.png|'''ClustalW'''<br />
File:muscle_bl.png |'''Muscle'''<br />
File:coffe_bl.png |'''T-Coffee'''<br />
</gallery><br />
<small>'''<caption>''' Trees of sequences with high similarity (set1) paired by average distance using BLOSUM62. </caption></small><br />
</figure><br />
<br />
<br />
By calculating the trees <xr id="ad"/> with the average distance using BLOSUM62, all three trees separate the whale proteins into one subtree. They differ in their pairing of the remaining sequences. Interestingly, the trees of ClustalW and T-Coffee are not the same anymore, as the trees paired according to their average distance using %identity. Now the alignments of Muscle and T-Coffee result to the exact same tree. While Muscle and T-Coffee group the glucocerebrosidase with the horse and bovine protein, ClustalW groups all human proteins together.<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Organisms of Uniprot Entries<br />
|- <br />
! style="background:#efefef;" align="center" | Entry<br />
! style="background:#efefef;" align="center" | Organism<br />
|-<br />
|HUMAN || Human<br />
|-<br />
|HORSE || Horse<br />
|-<br />
|BOVIN || Bovine<br />
|-<br />
|BALPH || Finback whale<br />
|-<br />
|DELDE || Saddleback dolphin<br />
|-<br />
|DELCA || Long-beaked common dolphin<br />
|-<br />
|}<br />
<br />
===For future research===<br />
Multiple sequence alignments are suitable to detect conserved regions in proteins. As for such researches, sequences with high similarity cause proper results.<br />
For a good alignment of very similar sequences, we would expect few gaps and many conserved columns of residues. However, we would only focus on gaps inside the sequences and neglect the gaps at the beginning and the end of sequences. Under these categories, we would use and advise Espresso. It shows more conserved columns than Muscle and T-Coffee and less gaps than ClustalW. Even the fact that an alpha helix is cut by a very long contiguous gap in the secondary structure, also convinces to use Espresso instead of ClustalW. The Espresso generated alignment is a compact alignment which provides a conserved region.<br />
<br />
In case of having only sequences with low similarity, we would tend to the use of ClustalW. The analyses with set 2 and set 3 show better results for alignments generated by ClustalW. These Alignments have less gaps and less straightened than the alignments produced by Muscle, T-Coffee and Espresso.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_02_-_Alignments&diff=38905
Gaucher Disease: Task 02 - Alignments
2013-09-06T00:36:28Z
<p>Kalemanovm: /* Multiple Sequence Alignment */</p>
<hr />
<div>Alignments allow a comparisons of Strings. In the field of bioinformatics, sequence alignments show the relation between two or more sequences. <br />
==Theoretical Background==<br />
<br />
===Description===<br />
Pairwise or multiple alignments often contain an additional line below the proper alignment or between two aligned sequences. This line gives a more accurate description of the relation of the aligned residues. The symbols show if there is match between identical amino acids or if they are only similar.<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Symbols for describing sequence alignments<br />
|-<br />
! style="background:#efefef;" align="center" | Program(s)<br />
! style="background:#efefef;" align="center" | Symbol<br />
! style="background:#efefef;" align="center" | Example<br />
! style="background:#efefef;" align="center" | Meaning<br />
|-<br />
| align="center" rowspan="3" | MSAs<br />
| align="center" |*<br />
| align="center" |A<br>A<br>*<br />
| align="center" |identical residues<br />
|-<br />
| align="center" |:<br />
| align="center" |I<br>L<br>:<br />
| align="center" |similar residues<br />
|-<br />
| align="center" |.<br />
| align="center" |S<br>P<br>.<br />
| align="center" |similar residues<br />
|-<br />
| align="center" rowspan="2" | (Psi-)BLAST<br />
| align="center" |same letter <br />
| align="center" |A<br>A<br>A<br />
| align="center" |identical residues<br />
|-<br />
| align="center" |+<br />
| align="center" |L<br>+<br>V<br />
| align="center" |similar residues<br />
|-<br />
| align="center" rowspan="3" | HHblits<br />
| align="center" |<nowiki>|</nowiki><br />
| align="center" |AF<br><nowiki>| |</nowiki><br>AW<br />
| align="center" |identical or very similar residues<br />
|-<br />
| align="center" |+<br />
| align="center" |T<br>+<br>S<br />
| align="center" |similar residues<br />
|-<br />
| align="center" |.<br />
| align="center" |N<br>.<br>H<br />
| align="center" |non-similar residues<br />
|-<br />
|}<br />
<br />
==Sequence Searches==<br />
<br />
===Runs===<br />
Sequence searches with our query protein sequence - enzyme glucocerebrosidase, P04062.fasta, were done with the following programs:<br />
<br />
* '''BLAST''' <br />
** using standard parameters<br />
** against big_80<br />
** against big<br />
<br />
* '''Psi-BLAST''' <br />
** with number of shown hits and alignments set to 10000 (-b, -v options), so that all the hits will be shown. <br />
** with all combinations of:<br />
*** 2 iterations: 1 iterations against big_80 followed by 1 iteration against big<br />
*** 10 iterations: 9 iterations against big_80 followed by 1 iteration against big<br />
*** default E-value cutoff (0.002)<br />
*** E-value cutoff 10E-10<br />
** other options leaved default<br />
<br />
* '''HHblits'''<br />
** with number of shown hits and alignments set to 10000 (-Z, -B options), as in Psi-BLAST <br />
** with all combinations of:<br />
*** 2 iterations against uniprot_20<br />
*** 10 iterations against uniprot_20<br />
*** default E-value cutoff (0.001)<br />
*** E-value cutoff 10E-10<br />
** other options leaved default<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
HHblits searches among representative sequences from different uniprot_20 clusters. When a representative from one cluster is found, all the sequences from this cluster are being added to the hit list. Thus HHblits finds much more hits than PSI-BLAST. Therefore, one additional PSI-BLAST run against the unfiltered big database was performed, using checkpoint files of the last profile created from the previous search in big80, in order to find as much related sequences as possible, as HHblits does indirectly. Summing up, the following databases were used: big_80 for profile building and big for the last iteration for PSI-BLAST and uniprot20 for HHblits. BLAST runs (consisting only from one iteration), we searched once against big_80 and once against big to see the difference in number of found hits.<br />
<br />
===Comparison===<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
====Number and overlap of hits====<br />
<br />
Number of found hits with each program and parameter combination and overlap of hits between some are summarized in <xr id="hit"/>.<br />
<br />
<figtable id="hit"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Number and overlap of hits<br />
|-<br />
! style="background:#efefef;" align="center" | Program<br />
! style="background:#efefef;" align="center" | Settings<br />
! style="background:#efefef;" align="center" | Number of hits<br />
! style="background:#efefef;" align="center" | Of them overlapped - with ...<br />
|-<br />
|align="center" rowspan="2" | BLAST<br />
|big_80<br />
|569<br />
|310 - both HHblits searches with E-val 0.001<br />
|-<br />
|big<br />
|1209<br />
|643 - both HHblits searches with E-val 0.001<br />
|-<br />
|align="center" rowspan="9" | Psi-BLAST<br />
|all combinations with 1 iter big<br />
|1216<br />
|644 - HHblits search with same combination<br />
|-<br />
|1 iter big_80 + 2 iter big, default E-val<br />
|2274<br />
|1126 - HHblits 2 iter, default E-val<br />
|-<br />
|1 iter big_80 + 2 iter big, E-val 10E-10<br />
|2099<br />
|1071 - HHblits 2 iter, E-val 10E-10<br />
|-<br />
|9 iter big_80 + 2 iter big, default E-val<br />
|2274<br />
|1174 - HHblits 10 iter, default E-val<br />
|-<br />
|9 iter big_80 + 2 iter big, E-val 10E-10<br />
|2099<br />
|1069 - HHblits 10 iter, E-val 10E-10<br />
|-<br />
|2 iter big_80, default E-val<br />
|1092<br />
|<br />
|-<br />
|2 iter big_80, E-val 10E-10<br />
|987<br />
|<br />
|-<br />
|10 iter big_80, default E-val<br />
|4031<br />
|<br />
|-<br />
|10 iter big_80, E-val 10E-10<br />
|1525<br />
|<br />
|-<br />
|align="center" rowspan="4" | HHblits<br />
|2 iter., E-val 0.001<br />
|1985<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|2 iter., E-val 10E-10<br />
|1922<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|10 iter., E-val 0.001<br />
|17538<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|10 iter., E-val 10E-10<br />
|3189<br />
|644 - Psi-BLAST search with same combination<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Number and overlap of hits from Blast, Psi-BLAST and HHblits.</caption></small></center><br />
</figtable><br />
<br />
'''Observations'''<br />
*BLAST search against not filtered big yields significantly more hits than against the redundancy reduced big_80.<br />
*Iterative BLAST (i.e. Psi-BLAST) finds far more hits than BLAST.<br />
*HHblits finds more hits than BLAST and Psi-BLAST (latter when number of iterations comparable).<br />
*HHblits finds more hits after 10 iterations than after 2 iterations.<br />
*HHblits finds less hits when lower E-value cutoff (10E-10) is used. For 2 iterations it is a little less but for 10 iterations it is much less.<br />
*The "high outlier" is number of hits with HHblits search with 10 iterations and default E-value inclusion cutoff.<br />
*Psi-BLAST number of hits is the same in all 4 parameter combinations after 1 last iteration against big. Thought after another iteration against big (done because of the check files), the number of hits using the default E-value cutoff is higher than using the E-value cutoff 10E-10. However, the number of hits stays the same independent of the number of "profile building" iterations against big_80 (1 or 9). In the second iteration against big much more hits are found, almost the double. -> Is one iteration against big not enough to "unfold" the profile?<br />
*Psi-BLAST after the 4 different runs only against big_80 are summarized in the table. The trends of the number of hits are comparable to HHblits (more hits with more iterations and less hits with the more restrictive E-value).<br />
*Number of hits found by both HHblits and Psi-BLAST or both HHblits and BLAST is almost the same and independent of the search parameters we used (therefore in Psi-BLAST the same hits are found after 1 iteration against big with all parameter combinations). But the overlap is significantly higher after 1 additional Psi-BLAST iteration against big.<br />
<br />
To gain more understanding about the found hits using different programs and search options, E-value, percentage identity and alignment length distributions were plottet.<br />
<br />
====E-value distribution====<br />
<figure id="evalue"><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_evalue_hist_log_freq.png|E-value distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis -log of the E-value is plotted, so the smallest (the best) E-value is on the right side. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' E-value distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST has a similar E-value distribution as Psi-BLAST.<br />
*Same range of E-value for (Psi-)BLAST and HHblits, however, different frequency of different values: (Psi-)BLAST has more higher E-values (left side) while HHblits has two peaks at high and low values.<br />
*For a high number of iterations, HHblits finds more hits with lower E-values (especially with default E-value cutoff).<br />
<br />
====Percentage identity distribution====<br />
<figure id="identity" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_identity_hist.png|Identity distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_identity_hist.png|Identity distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_identity_hist.png|Identity distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_identity_hist.png|Identity distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the Y-axis is frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' Identity distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST and Psi-BLAST have similar identity distributions, with peaks at about 30%, 85% and 100%.<br />
*HHblits has another high peak at about 20%, which moves to even lower identities with higher number of iterations. This peak is especially high with 100 iterations and default E-value cutoff, because of the high number of found hits.<br />
<br />
====Alignment length distribution====<br />
<figure id="length" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20.hhr_results.psiblast-1iter-big_80--1iter_big_results.blast-big_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (2 iterations against uniprot_20 with default E-value cutoff, 0.001), Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002) and BLAST hits (against big with default parameters). On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-2iter-uniprot20-10E-10.hhr_results.psiblast-1iter-big_80--1iter_big-10E-10_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (2 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (1 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20.hhr_results.psiblast-9iter-big_80--1iter_big_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (10 iterations against uniprot_20 with default E-value cutoff, 0.001) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with default E-value cutoff, 0.002). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
File:hhblits-10iter-uniprot20-10E-10.hhr_results.psiblast-9iter-big_80--1iter_big-10E-10_results_length_hist_log_freq.png|Alignment length distribution of HHblits hits (10 iterations against uniprot_20 with E-value cutoff 10E-10) and Psi-BLAST hits (9 iteration against big_80 followed by one iteration against big with E-value cutoff 10E-10). On the X-axis %identity is plotted [0,1]. On the X-axis alignment length is plotted. On the Y-axis is logarithmic frequency of the values on the X-axis.<br />
</gallery><br />
<small>'''<caption>''' Alignment length distribution of BLAST and HHblits and Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*BLAST and Psi-BLAST have similar alignment length distributions.<br />
*HHblits and (Psi-)BLAST alignment lengths have similar range from 1 to round 550 residues.<br />
* In general, (Psi-)BLAST seems to produce longer alignments than HHblits.<br />
<br />
===Evaluation===<br />
<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
====Results====<br />
<br />
Resulting plots of TPR and precision as a function of E-value are shown in <xr id="blast_results"/>, <xr id="psiblast_results"/> and <xr id="hhblits_results"/>.<br />
<br />
'''BLAST'''<br />
<figure id="blast_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:blast-big_80_eval_tpr_precision.png|TPR and precision vs. E-value of BLAST hits after 1 big_80 iteration with default parameters using the L30, L40 and L60 COPS groups as gold standard. On the X-axis E-value is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:blast-big_eval_tpr_precision.png|TPR and precision vs. E-value of BLAST hits after 1 big iteration with default parameters using the L30, L40 and L60 COPS groups as gold standard. On the X-axis E-value is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of BLAST hits searched in big_80 and big database.</caption></small><br />
</figure><br />
<br />
'''Psi-BLAST'''<br />
<figure id="psiblast_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:psiblast-1iter-big_80--1iter_big--1iter_pdb_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 1 big_80, 1 big and 1 pdb_full iterations with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-1iter-big_80--1iter_big--1iter_pdb-10E-10_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 1 big_80, 1 big and 1 pdb_full iterations with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-9iter-big_80--1iter_big--1iter_pdb_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 9 big_80, 1 big and 1 pdb_full iterations with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:psiblast-9iter-big_80--1iter_big--1iter_pdb-10E-10_eval_tpr_precision.png|TPR and precision vs. E-value of Psi-BLAST hits after 9 big_80, 1 big and 1 pdb_full iterations with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of Psi-BLAST hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''HHblits'''<br />
<figure id="hhblits_results" ><br />
<gallery widths="500px" heights="500px" perrow="2"><br />
File:hhblits-2iter-uniprot20--1iter-pdb.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 2 uniprot_20 iterations and 1 pdb_full iteration with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-2iter-uniprot20--1iter-pdb-10E-10.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 2 uniprot_20 iterations and 1 pdb_full iteration with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-10iter-uniprot20--1iter-pdb.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 10 uniprot_20 iterations and 1 pdb_full iteration with default E-value using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
File:hhblits-10iter-uniprot20--1iter-pdb-10E-10.hhr_eval_tpr_precision.png|TPR and precision vs. E-value of HHblits hits after 10 uniprot_20 iterations and 1 pdb_full iteration with E-value 10E-10 using the L30, L40 and L60 COPS groups as gold standard. On the X-axis -log(E-value) is plotted. On the Y-axis are percentual values of TPR and precision of all hits until respective E-value cutoff.<br />
</gallery><br />
<small>'''<caption>''' TPR and precision vs. E-value of HHblits hits with different combinations of number of iterations and E-value cutoffs.</caption></small><br />
</figure><br />
<br />
'''Observations'''<br />
<br />
*In '''BLAST''' search against '''big_80''', only one hit was found. It has the same L60 (therefore also L40 and L30) group as the query. It can be seen on the plot as a pair of points with the highest precision and lowest TPR.<br />
*In '''BLAST''' search against '''big''', only hits with two different E-values were found. Also there the precision is high but TPR low, especially for hits with same L30 group.<br />
*For the other programs - '''HHblits''' and '''Psi-BLAST''' - it can be observed, that TPR rises with growing E-value and precision drops with growing E-value, because then more TP and FP hits are found.<br />
**TPR rises with growing E-value, because more TP hits are detected, especially rapidly for L60. This indicates that more similar hits are detected more easily, almost all L60 are found. However only a small subset of L40 and L30 hits are retrieved. The TPR plot is similar for Psi-BLAST and HHblits searches. '''HHblits''' achieves slightly higher precision then '''Psi-BLAST''': 24% vs. 9% for L30, 40% vs. 15% for L40. Both programs find all L60 hits.<br />
**There is a different behavior in precision:<br />
***In '''Psi-BLAST''' searches, precision stays 100% until approx. E-value 10E-20, then it starts to drop, more rapidly for L60 group than for L40 and L30 groups. It shows that hits with high E-values probably are not very similar to the query. <br />
***In '''HHblits''' searches, precision is 100% for very low E-values, then as E-value grows precision of all structural groups falls rapidly to about 90% and stays there for a while. After some E-value is achieved (usually approx. > 10E-10), precision continues to drop further. Also here precision for L60 groups falls more rapidly than for L40 and L30 groups.<br />
** '''Psi-BLAST''' results for different settings are overall very similar, except that less TP are found with E-value inclusion cutoff 10E-10.<br />
**For '''HHblits''' there is one exeption: the plot for HHblits with 10 iterations and default E-value cutoff (for inclusion into profile) looks very different. The highest TPR values are achieved: 30% for L30, 52% for L40 and 100% for L60, however the precision drops not only already at very low E-values to about 90%, as also for other HHblits searches, but it continues to drop already after E-value 10E-30 for all the structural groups (again more rapidly for L60). This indicates that many HHblits iterations combined with not very restrictive E-value inclusion cutoff 0.001 allow too many FP to penetrate into the profile and in the end into the result list. We do not recommend to use these search settings.<br />
**It could be a good idea to remove '''Psi-BLAST''' hits with E-value > 10E-20 and HHblits hits with E-value > 10E-10 (in most of the cases) to reduce the number of FP. However then we will loose some TP that are still found with these high E-values (L30 and L40).<br />
<br />
==Multiple Sequence Alignment==<br />
[[Task 02 - Lab Journal|Lab journal]]<br />
<br />
For the multiple sequence alignments, three sets were created. Therefore, the results of the previous task were used according to their sequence identity to the native protein sequence of glucocerebrosidase. Set 1 and set 2 contain 10 sequences each, including the native protein sequence to keep the alignments in relation to the Gaucher disease causing protein. The remaining 9 sequences have a sequence identity to the glucocerebrosidase of more than 60% in set 1 and less than 30% in set 2. In set 3, the 20 sequences are over the whole range of sequence identity. <br />
<br />
In the following analyses the term "conserved region" is used for a section of columns that feature a higher number of conserved columns or columns of similar residues. Moreover, the term "contiguous gap" is used for an amount of consecutive gaps that split up the protein sequence.<br />
<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="left"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Set 1: sequence identity >60%<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|-<br />
|A9UD35<br />
|84.1<br />
|-<br />
|D1L2S0<br />
|83.0<br />
|-<br />
|3gxi_A<br />
|99.8<br />
|-<br />
|2nt1_A<br />
|99.8<br />
|-<br />
|F6WDY8<br />
|90.7<br />
|-<br />
|Q2KHZ8<br />
|89.2<br />
|-<br />
|F5CB27<br />
|81.8<br />
|-<br />
|F5H241<br />
|98.2<br />
|-<br />
|B7Z6S9<br />
|99.8<br />
|-<br />
|}<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="align"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Set 2: sequence identity <30%<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|-<br />
|H6CEV7<br />
|26.5<br />
|-<br />
|Q21GD0<br />
|24.3<br />
|-<br />
|I1WBF3<br />
|23.7<br />
|-<br />
|I9HH59<br />
|29.4<br />
|-<br />
|D0TN48<br />
|25.4<br />
|-<br />
|B1VPJ0<br />
|27.5<br />
|-<br />
|E2LY19<br />
|24.8<br />
|-<br />
|K9HBW2<br />
|25.9<br />
|-<br />
|B5QQZ8<br />
|27.1<br />
|-<br />
|}<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="align"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Set 3: sequence identity over all<br />
|-<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
! style="background:#efefef;" align="center" | ID<br />
! style="background:#efefef;" align="center" | Identity in %<br />
|-<br />
|P04062<br />
|100 <br />
|B5DYA3<br />
|32.0<br />
|-<br />
|B4JTN5<br />
|34.1<br />
|F5CB37<br />
|81.8<br />
|-<br />
|E1ZYU8<br />
|42.7<br />
|J2STU0<br />
|34.2<br />
|-<br />
|F4E6W5<br />
|26.4<br />
|G9BHQ3<br />
|80.7<br />
|-<br />
|F4X220<br />
|41.7<br />
|2nsx_B<br />
|99.8<br />
|-<br />
|E5UPZ0<br />
|21.6<br />
|G9BHQ5<br />
|82.5<br />
|-<br />
|C6A5Q0<br />
|25.0<br />
|D0TIX6<br />
|25.8<br />
|-<br />
|B1QKT0<br />
|27.8<br />
|1y7v_A<br />
|99.8<br />
|-<br />
|D4SV88<br />
|34.6<br />
|A9UD58<br />
|80.4<br />
|-<br />
|A9UD54<br />
|81.8<br />
|G9MUP6<br />
|20.7<br />
|-<br />
|}<br />
<br />
===ClustalW===<br />
<br />
Multiple alignments generated with ClustalW:<br />
<figure id="cl_1" ><br />
[[Image:fullrange_set1.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with ClustalW</caption>]]<br />
</figure><br />
<figure id="cl_2" ><br />
[[Image:set2_clustalw.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with ClustalW</caption>]]<br />
</figure><br />
<figure id="cl_3" ><br />
[[Image:set3_clustalw.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with ClustalW</caption>]]<br />
</figure><br />
<br />
<br />
'''Set 1''', which includes sequences with high similarity, has only 11 conserved columns. But these columns lie densely in an alignment area between position 284 and 337 (marked darkblue in <xr id="cl_1"/>). Also columns that are not conserved by identical residues, but have similar amino acids, can be found in this conserved region. This conserved area forms two alpha helices and one beta-sheet in its secondary structure. The gaps of the alignment split the protein into 5, parts of residues. Especially the last 400 residues have a very high sequence identity between the glucosylcerebrosidase and 6 other proteins. The last contiguous gap which is also the longest one lies inside an alpha helix of the secondary structure. <br />
<br />
'''Set 2''' has less conserved columns than set 1 (<xr id="cl_2"/>). They are spread over the alignment and build no conserved region. The contiguous gaps are more but shorter.<br />
<br />
There exist no conserved columns for '''set 3''' (<xr id="cl_3"/>). This could result from the greater number of sequences in the set. The more sequences are in an alignment the lesser the probability of having conserved columns. Furthermore, also the sequences with a low sequence identity cause to a loss of a conserved region. <br />
<br />
<br />
<figtable id="clu"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of ClustalW in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|11<br />
|8<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|3797<br />
|5239<br />
|6721<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|6<br />
|17<br />
|16<br />
|-<br />
|Alignment length<br />
|762<br />
|1079<br />
|722<br />
|}<br />
<center><small>'''<caption>''' ClustalW statistics of glucocerebrosidase.</caption></small></center><br />
</figtable><br />
<br />
===Muscle===<br />
Multiple alignments generated with Muscle:<br />
<figure id="m1" ><br />
[[Image:set1_muscle.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with Muscle</caption>]]<br />
</figure><br />
<figure id="m2" ><br />
[[Image:set2_muscle.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with Muscle</caption>]]<br />
</figure><br />
<figure id="m3" ><br />
[[Image:set3_muscle.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with Muscle</caption>]]<br />
</figure><br />
<br />
<br />
In '''set 1''' the native protein sequence itself has no gaps. There are only gaps at the beginning of the sequence, because the alignment (<xr id="m1"/>) is longer than the length of the glucocerebrosidase sequence. There are 10 conserved columns which all concentrate on the alignment positions between 138-204. The remaining residues of these area also have a high similarity between the different sequences. Only the sequence F5H241 is splitted by a few gaps at its beginning. This high conserved region affects a different region of the glucocerebrosidase (residues 53-119) than the high conservative region detected by ClustalW (residues 171-220).<br />
<br />
'''Set 2''' has also 10 conserved columns (<xr id="m2"/>). But in contrast to set 1, they are widely spread over the alignment. The same observation can be made about columns with similar residues. Through this scattering it seems rather a randomly generated conserved column than a conservation due to functional reasons. The high number as well as the partly great length of the contiguous gaps straighten the alignment. The alignment gives a great overview of the relation between sequences with low similarity and sequence identity. It also shows that for finding functional important areas that are conserved, a higher similarity and identity is needed.<br />
<br />
'''Set 3''' has no conserved columns (<xr id="m3"/>), for the same reason as explained before, for set 2. It has indeed the highest number of gaps of the three sets. But contrary to the alignment of set 2 the contiguous gaps are shorter, partially they are only 1-2 gaps long. So the alignment appears less straightened, as the sequences with high sequence identity keep the alignment a bit compact. <br />
<br />
<br />
<figtable id="mus"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Muscle in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|10<br />
|10<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|5609<br />
|7341<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|26<br />
|29<br />
|-<br />
|Alignment length <br />
|621<br />
|1116<br />
|753<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Muscle statistics of glucocerebrosidase. </caption></small></center><br />
</figtable><br />
<br />
===T-Coffee===<br />
Multiple alignments generated with T-Coffee:<br />
<figure id="tc1" ><br />
[[Image:set1_coffe.png|thumb|none|750px|'''<caption>''' '''Set1''': MSA of proteins with sequence identity >60% generated with T-Coffee</caption>]]<br />
</figure><br />
<figure id="tc2" ><br />
[[Image:set2_coffe.png|thumb|none|750px|'''<caption>''' '''Set2''': MSA of proteins with sequence identity <30% generated with T-Coffee</caption>]]<br />
</figure><br />
<figure id="tc3" ><br />
[[Image:set3_coffe.png|thumb|none|750px|'''<caption>''' '''Set3''': MSA of proteins with sequence identity over the whole range generated with T-Coffee</caption>]]<br />
</figure><br />
<br />
<br />
The Alignment of '''set 1''' in <xr id="tc1"/> generated by T-Coffee is very similar to its alignment made by Muscle. However, there exist no conserved columns. This is caused because of the shift of the sequences. So, in no alignment position, the residues of all sequences are aligned. The high conserved region of the Muscle-alignment is almost identical to a conserved region in the T-coffee generated alignment. They only differ in a shift of a part of sequence F5H241. The First few residues are now aligned at the beginning of the glucocerebrosidase instead of position 53. While Muscle includes gaps in this F5H241 part, T-Coffe does not separate the shifted residues.<br />
<br />
The other difference to the Muscle alignment is one long contiguous gap inside of the protein sequence A9UD35. This extremely long contiguous gap shifts the last amino acid of the sequence to the last position of the alignment. As this amino acid (G) is not identical to the remaining amino acids (Q) at the last alignment position of the other sequences, there seem to be no reason for this observation. The suspicion of an error arises, as the amino acid (G) would perfectly fit directly at the end of the sequence without a contiguous gap. <br />
<br />
'''Set 2''' shows widely spread conserved columns without building a conserved region. The alignment (<xr id="tc2"/>) itself has lots of gaps. An interpretation of the alignment in a biological way should be made carefully, as it bases on low sequence identity and similarity. Noticeable for this alignment is its beginning and end. All sequences are aligned in these areas with at least 4 residues. <br />
<br />
Sequences with an identity over the full range ('''set 3''') generate many gaps of different length and no conserved columns (<xr id="tc3"/>). Two different pattern are observable. On the one hand blocks of aligned residues are separated mostly by a block of gaps and are only connected by 1-2 sequences (for example positions 495-515). On the other hand a block is not completely aligned by residues of all sequences, but has an contiguous gap of a few sequences (for example positions 415-450). <br />
<br />
<br />
<figtable id="cof"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Muscle in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|0<br />
|12<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|6719<br />
|9361<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|44<br />
|50<br />
|-<br />
|Alignment length <br />
|621<br />
|1227<br />
|854<br />
|-<br />
|}<br />
<center><small>'''<caption>''' T-Coffee statistics of glucocerebrosidase.</caption></small></center><br />
</figtable><br />
<br />
===Espresso===<br />
Espresso is an advanced program of T-Coffee. While T-Coffee only uses sequences, Espresso also takes the 3D structure of the proteins into account.<br />
<br />
Multiple alignments generated with Espresso:<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/80555308/result.score_html Set1] (>60% seq.identity to glucocerbrosidase)<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/ccc1fd04/result.score_html Set2] (<30% seq.identity to glucocerbrosidase)<br />
* Results for Espresso of : [http://tcoffee.crg.cat/data/4903ac99/result.score_html Set3] (all % seq.identity to glucocerbrosidase)<br />
(Please note: in case of an unavailability of the alignments in the links above, please contact the author)<br />
<br />
The additional use of the structure improves the alignment of sequences with high sequence identity ('''set 1'''). In contrary to T-Coffee, Espresso detects now conserved columns. Through a little difference in the alignment score calculating, the residues in the beginning of the F5H241 aligne different than in T-Coffee. Now an conserved area between residues 53 and 119, including 12 high conserved columns and 9 columns with similar amino acids, can be seen in the alignment. The residues are now aligned not equal but similar and better to those in the Muscle-alignment. These conserved area forms four beta-sheets in its secondary structure.<br />
Also the last residue of A9UD35 which was mentioned before in connection with T-Coffee is now aligned directly at the end of its sequence.<br />
<br />
The two other sets containing also sequences with low sequence identity show no remarkable improvement to the results of T-Coffee .<br />
<br />
<br />
<figtable id="es"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Results of Espresso in numbers<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Set 1<br />
! style="background:#efefef;" align="center" | Set 2<br />
! style="background:#efefef;" align="center" | Set 3<br />
|-<br />
|Conserved Columns<br />
|12<br />
|11<br />
|0<br />
|-<br />
|Number of gaps in the Alignm.<br />
|2387<br />
|6769<br />
|9341<br />
|-<br />
|Number of contiguous gaps in P04062 <br />
|0<br />
|46<br />
|51<br />
|-<br />
|Alignment length <br />
|621<br />
|1232<br />
|853<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Espresso statistics of glucocerebrosidase</caption></small></center><br />
</figtable><br />
<br />
===Note===<br />
The generated T-Coffee and Espresso alignments of set 2 and 3 seem to focus aligned residues at their beginning or their end. So the question arises: Why? Why are these residues aligned, even though enormous gap penalty occurs considering the resulting long contiguous gaps. One thing is certain: T-Coffee does not use a freeshift alignments for generating a multiple alignment.<br />
<br />
===Alignment Comparison===<br />
Alignment comparison of the MSA of sequences with high similarity ('''Set 1''') generated by the programs described above.<br />
<br />
The Alignments produced by Muscle, T-Coffee and Espresso are very similar. They only differ in their aligning of the sequence F5H241. In case of Espresso the sequence was aligned so that a conserved region is observable. The conserved region, detected by ClustalW is on a different position. <br />
<br />
Nevertheless the resulting trees in <xr id="sid"/> based on the Average % of sequence identity differ from each other.<br />
<br />
<figure id="sid" ><br />
<gallery widths=285px heights=200px caption=""><br />
File:clustal_seqid.png|'''ClustalW'''<br />
File:muscle_seqid.png |'''Muscle'''<br />
File:coffe_seqid.png |'''T-Coffee'''<br />
</gallery><br />
<small>'''<caption>''' Trees of sequences with high similarity (set1) paired by average distance using %Identity. </caption></small><br />
</figure><br />
<br />
<br />
The trees (<xr id="sid"/>) based on the alignment generated by ClustalW and T-Coffee are identically in their sequence pairing. All human proteins can be found in one subtree. The same proteins are paired in both trees, just as the remaining mammalian proteins do in the other subtree. They only differ in their distances between paired sequences. This is an unexpected observation, as both alignments differ especially in their conserved region. <br />
But the tree showing the alignment from Muscle deviates enormously from the other two trees. Indeed, the mammalian subtree coincide with the others, but the human proteins do not. The human protein F5H241 which is directly paired to the glucocerebrosidase by ClustalW and T-Coffee is now linked (yellow) to the subtree consisting of all proteins (red, blue). This is the protein which is aligned different for Muscle and T-Coffee. So Muscle shows a different %Identity between the aligned sequences than ClustalW or T-Coffee.<br />
<br />
<br />
<figure id="ad" ><br />
<gallery widths=285px heights=200px caption=""><br />
File:clustal_bl.png|'''ClustalW'''<br />
File:muscle_bl.png |'''Muscle'''<br />
File:coffe_bl.png |'''T-Coffee'''<br />
</gallery><br />
<small>'''<caption>''' Trees of sequences with high similarity (set1) paired by average distance using BLOSUM62. </caption></small><br />
</figure><br />
<br />
<br />
By calculating the trees <xr id="ad"/> with the average distance using BLOSUM62, all three trees separate the whale proteins into one subtree. They differ in their pairing of the remaining sequences. Interestingly, the trees of ClustalW and T-Coffee are not the same anymore, as the trees paired according to their average distance using %identity. Now the alignments of Muscle and T-Coffee result to the exact same tree. While Muscle and T-Coffee group the glucocerebrosidase with the horse and bovine protein, ClustalW groups all human proteins together.<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Organisms of Uniprot Entries<br />
|- <br />
! style="background:#efefef;" align="center" | Entry<br />
! style="background:#efefef;" align="center" | Organism<br />
|-<br />
|HUMAN || Human<br />
|-<br />
|HORSE || Horse<br />
|-<br />
|BOVIN || Bovine<br />
|-<br />
|BALPH || Finback whale<br />
|-<br />
|DELDE || Saddleback dolphin<br />
|-<br />
|DELCA || Long-beaked common dolphin<br />
|-<br />
|}<br />
<br />
===For future research===<br />
Multiple sequence alignments are suitable to detect conserved regions in proteins. As for such researches, sequences with high similarity cause proper results.<br />
For a good alignment of very similar sequences, we would expect few gaps and many conserved columns of residues. However, we would only focus on gaps inside the sequences and neglect the gaps at the beginning and the end of sequences. Under these categories, we would use and advise Espresso. It shows more conserved columns than Muscle and T-Coffee and less gaps than ClustalW. Even the fact that an alpha helix is cut by a very long contiguous gap in the secondary structure, also convinces to use Espresso instead of ClustalW. The Espresso generated alignment is a compact alignment which provides a conserved region.<br />
<br />
In case of having only sequences with low similarity, we would tend to the use of ClustalW. The analyses with set 2 and set 3 show better results for alignments generated by ClustalW. These Alignments have less gaps and less straightened than the alignments produced by Muscle, T-Coffee and Espresso.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_02_-_Lab_Journal&diff=38904
Gaucher Disease: Task 02 - Lab Journal
2013-09-06T00:29:52Z
<p>Kalemanovm: </p>
<hr />
<div>==Sequence Searches==<br />
The scripts written for this task can be found in <code>/mnt/home/student/kalemanovm/master_practical/Assignment2_Alignments/scripts/task1</code>, the created outputs in <code>/mnt/home/student/kalemanovm/master_practical/Assignment2_Alignments/output/task1</code> and all intermediate and resulting files in <code>/mnt/home/student/kalemanovm/master_practical/Assignment2_Alignments/results/task1</code>.<br />
<br />
===Runs===<br />
The script [[run.pl]] was written and used for the runs (using the bash script <code>run.sh</code>). PSSM files - a3m and hhr for HHblits, chk ("checkpoint") and PSSM for Psi-BLAST were created in order to start the search against another database, from big_80 to big for Psi-BLAST and later against a PDB database for the evaluation.<br />
'''Note''': in Psi-BLAST in order to create a checkpoint file, one iteration more must be done, as the checkpoint file is created at the beginning of a iteration, not at its end. For example, to resume 9 iterations against big_80 with 1 iteration against big and then with 1 iteration against pdb_seqres the following calls were made (from <code>~/master_practical/Assignment2_Alignments/output/task1</code>): <br><br />
<br />
../../scripts/task1/run.pl --in ../../gaucher_data/P04062.fasta --out_p psiblast-10iter-big_80 --iter_p 10 --db_b big_80 --c psiblast-9iter-big_80.chk<br />
<br />
../../scripts/task1/run.pl --in ../../gaucher_data/P04062.fasta --out_p psiblast-9iter-big_80--2iter_big --iter_p 2 --db_b big <br />
--r psiblast-9iter-big_80.chk --c psiblast-9iter-big_80--1iter_big.chk<br />
<br />
../../scripts/task1/run.pl --in ../../gaucher_data/P04062.fasta --out_p psiblast-9iter-big_80--1iter_big--1iter_pdb --db_b pdb_seqres --iter_p 1 <br />
--r psiblast-9iter-big_80--1iter_big.chk<br />
<br />
====Databases====<br />
We have used the following databases versions:<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#efefef;" align="center" | Database<br />
! style="background:#efefef;" align="center" | Location<br />
! style="background:#efefef;" align="center" | Version<br />
|-<br />
| align="center" |big & big_80<br />
| align="center" |/mnt/project/rost_db/data/big/big[_80]<br />
| align="center" |before 30 Apr 2013?<br />
|-<br />
| align="center" |uniprot_20<br />
| align="center" |/mnt/project/rost_db/data/hhblits/uniprot_20<br />
| align="center" |02 Sep 2011<br />
|-<br />
| align="center" |pdb_seqres <br />
| align="center" |/mnt/home/rost/kloppmann/data/blast_db/pdb_seqres<br />
| align="center" |3 May 2013<br />
|-<br />
| align="center" |pdb_full<br />
| align="center" |/mnt/project/rost_db/data/hhblits/pdb_full<br />
| align="center" |before 30 Apr 2013?<br />
|-<br />
| align="center" |COPS<br />
| align="center" |/mnt/project/pracstrucfunc13/data/COPS/COPS-ChainHierarchy.txt<br />
| align="center" |27 Apr 2012<br />
|-<br />
|}<br />
<br />
===Comparison===<br />
The results were parsed and analyzed using the script [[parse_output.pl]].<br />
The distributions of E-value, sequence identity and alignment length were plotted using the R-scripts <code>hist_eval_iden_len.r</code> and <code>hist_eval_iden_len-with_blast.r</code><br />
<br />
===Evaluation===<br />
====Method====<br />
*The quality of hits retrieved by HHblits, PSI-BLAST and BLAST was evaluated using the COPS L30/L40/L60 structural similarity groups as standard of truth. Protein chains with same L30/L40/L60 group have 30/40/60% structural similarity, respectively. For example, when using the L30 group as standard of truth, the hit is a true positive (TP) if it has the same L30 group as the query, otherwise it is a false positive (FP).<br />
*The query structure 1OGS chain A of glucocerebrosidase was used for comparison with the following COPS groups: <br />
**L30 of query: 3zr6_A <br />
**L40 of query: 3ii1_A <br />
**L60 of query: 2v3e_B <br />
*The total number of chains in COPS with the same L30/L40/L60 group as the query is calculated and stored:<br />
**L30: 2058<br />
**L40: 1126<br />
**L60: 80<br />
*Parsed results of the runs, also with the script [[parse_output.pl]] (using the bash script <code>parse_output.sh</code>), include the following information for each hit (as mentioned, in case of more then one alignment of the query and the same found sequence, only the one with the lowest E-value was used): E-value, whether the hit is a TP or a FP, query ID and hit ID. <br />
*Then the evaluation routine [[tpr_precision.pl]] was applied (using the bash script <code>tpr_precision.sh</code>). In the evaluation routine, each file is first sorted according to ascending E-value. Afterwards, the following rates are calculated for each line (from the first to the current line): TPR (sensitivity) and precision. Each rate is calculated specifically for each query seen so far, and then the average is taken (sum of all rates for each query divided by the number of the queries seen so far). The rates are defined as follows: <br><br />
<center><br />
TPR = TP/(TP+FN) <br><br />
precision = TP/(TP+FP) <br><br />
</center><br />
where:<br />
* TP = the number of true positive hits, namely all found hits with the same COPS L30/L40/L60 group as the query<br />
* FP = the number of false positive hits, that is with different groups than the query<br />
* FN = the number of false negatives, which are not detected chains with the same group as the query<br />
* TP+FN = the number of all positives, i.e. the number of all known PDB chains with the same group as the query<br />
* TP+FP = the number of all found hits<br />
<br />
<br />
==Multiple Sequence Alignment==<br />
<br />
===Sets===<br />
The three sets containing 10 or 20 sequences were extracted from the HHblits results of exercise 1 (Sequence Searches) with 10 iterations against uniprot_20 with an E-value cutoff 10E-10.<br><br />
All sets were compiled with <code>/mnt/home/student/gerkej/gaucher/task2/parseSets.py</code>.<br />
<br />
===Program in detail===<br />
<code>parseSets.py</code> splits the HHblits file into 3 parts. One part contains all sequences with an sequence identity >60%. The next part includes all sequences below the 30% threshold. The remaining sequences were joined in the last part. While Set 1 and Set 2 pull random sequences out of the corresponding part, Set 3 is generated differently. To guarantee a constant distribution of the sequence identities within the 20 sequences, 1/3 of the sequences were randomly taken of each of the three parts.<br />
<br />
===Alignments===<br />
The multiple sequence alignments (MSA) were generated and visualized with JalView. JalView provides a web service for ClustalW, Muscle and T-Coffee. Espresso was run on the web server. Espresso searches automatically the PDB with BLAST to extract the structures of the sequences. The visualization was generated of the program itself. Similarity trees were also generated with JalView. Therefore, the average distance was used with both %identity and Blosum62 as "scoring matrix". <br />
<br />
The used versions: <br />
<br />
*ClustalW: version 2.1<br />
*Muscle: version 3.8.31<br />
*T-Coffee: version 8.99<br />
*[http://tcoffee.crg.cat/apps/tcoffee/do:expresso Espresso]: version 9.03<br />
<br />
For a closer look at the secondary structure that might be affected by an alignment gap, the PDB entry 1OGS was used.</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Lab_Journal&diff=38903
Gaucher Disease: Task 03 - Lab Journal
2013-09-06T00:27:32Z
<p>Kalemanovm: </p>
<hr />
<div><css><br />
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/* for orange try #ff7f00 and #ffaa56<br />
for blue try #005fbf and #aad4ff<br />
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maria's style<br />
blue: #adceff<br />
grey: #efefef<br />
*/<br />
.colBasic2 tr th { background-color:#efefef; color: black;}<br />
.colBasic2 tr:first-child th { background-color:#adceff; color:black;}<br />
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<br />
<br />
== Secondary structure ==<br />
<br />
'''ReProf''' uses a fasta sequence or a PSI-BLAST PSSM for prediction, '''PsiPred''' a fasta sequence and '''DSSP''' server needs a PDB file in order to use the 3D coordinates of atoms. The predictions were made for the four proteins, including the Gaucher's disease-causing protein (<xr id="secondary structure proteins"/>). If several PDB structures are available, the one covering the most UniProt sequence most similarly was chosen. For glucosylceramidase the structure 1OGS was used (as in the task 2).<br />
<br />
<figtable id="secondary structure proteins"><br />
{| class="colBasic2"<br />
! colspan="4" | Uniprot<br />
! colspan="5" | PDB<br />
|-<br />
! Entry || Protein name || Origin || Length || Entry || Method || Resolution (Å) || Chain || Positions<br />
|-<br />
|[http://www.uniprot.org/uniprot/P10775 P10775] || Ribonuclease inhibitor || pig || 456 || [http://www.pdb.org/pdb/explore/explore.do?pdbId=2BNH 2BNH] || X-ray || 2.30 || A || 1-456<br />
|-<br />
|[http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6] || Divalent-cation tolerance protein CutA || bacterium Thermotoga maritima || 101 || [http://www.pdb.org/pdb/explore/explore.do?pdbId=1VHF 1VHF] || X-ray || 1.54 || A || 2-101<br />
|-<br />
|[http://www.uniprot.org/uniprot/Q08209 Q08209] || Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, EC=3.1.3.16 || human || 521 || [http://www.pdb.org/pdb/explore/explore.do?pdbId=1AUI 1AUI] || X-ray || 2.10 || A || 1-521<br />
|-<br />
|[http://www.uniprot.org/uniprot/P04062 P04062] || Glucosylceramidase/acid-beta-glucosidase, EC=3.2.1.45 || human || 536 || [http://www.pdb.org/pdb/explore/explore.do?pdbId=1OGS 1OGS] || X-ray || 2.00 || A/B || 40-536<br />
|-<br />
|}<br />
<center><small>'''<caption>''' The four UniProt protein sequences and corresponding PDB structures selected for the secondary structure prediction with ReProf and PsiPred and assignment with DSSP.</caption></small></center><br />
</figtable><br />
<br />
The script of the Phenylketonuria group [[Phenylketonuria/Task3/Scripts | filter_secStruc.pl]] was used to extract the secondary structures in the three letter code: E, H and L (<xr id="secondary structure code"/>). For DSSP irregular regions are encoded as "-". Then, precision for each one of the three secondary structures and in total, ignoring positions without a secondary structure ("-"), was calculated using the second script of the Phenylketonuria group, [[Phenylketonuria/Task3/Scripts | SecStrucComparison.jar]] as follows:<br />
<br />
<code><center>precision = number of matches / number of residues </center></code><br />
<br />
<figtable id="secondary structure code"><br />
{| class="colBasic2"<br />
|-<br />
! colspan="4" | "Secondary Structure Code" <br />
|-<br />
! Secondary structure || ReProf || PsiPred || DSSP<br />
|-<br />
| Helix (alpha) || H || H || GHI<br />
|-<br />
| Extended strand (beta) || E || E || BE<br />
|-<br />
| Loops/Turns || L || C || ST<br />
|-<br />
|}<br />
<center><small>'''<caption>''' The different types to represent secondary structure in ReProf, PsiPred and DSSP.</caption></small></center><br />
</figtable><br />
<br />
First different PSSMs after different PSI-BLAST runs (all combinations: against big_80/swissprot database, 2/3 iterations, E-value 2E-3/10E-10/10E-20) were tested on the shortest protein, Q9X0E6, then the run parameters yielding the best precision compared to PsiPred and DSSP were chosen. The best parameters were: '''big_80, 3 iterations and evalue cutoff 10E-10''', which were then applied to create PSSMs for the other proteins. (The table where the results for all parameters are summarized can be seen here: <code>/mnt/home/student/kalemanovm/master_practical/Assignment3_SequenceBasedPredictions/SecondaryStructure/reprof_out/parsedSecStr/README.Q9X0E6.psiblast_param.precision</code>.)<br />
<br />
Other used scripts and created data can be found in: /mnt/home/student/kalemanovm/master_practical/Assignment3_SequenceBasedPredictions/SecondaryStructure.<br />
<br />
== Disorder ==<br />
'''IUPred'''<br />
We called IUPred with "long", "short" and "glob" modes the same four proteins (P10775, Q08209, Q9X0E6, P04062). One can generate graphical output with the [http://iupred.enzim.hu/ IUPred server]. However, for a clear view and interpretation of the results of the three options, we combined them in a single plot with an R script. <br />
<br />
<br />
'''MD (MetaDisorder)'''<br />
The output files ending on ".md.out.mdisorder" have the following columns (example for P10775):<br />
<br />
Number Residue NORSnet NORS2st PROFbval bval2st Ucon Ucon2st MD_raw MD_rel MD2st<br />
1 M 0.19 - 0.99 D 0.15 - 0.551 1 D<br />
2 N 0.11 - 0.78 D 0.21 - 0.475 1 -<br />
3 L 0.08 - 0.65 D 0.26 - 0.455 2 -<br />
4 D 0.13 - 0.68 D 0.20 - 0.414 3 -<br />
5 I 0.09 - 0.40 - 0.22 - 0.364 5 -<br />
6 H 0.14 - 0.48 D 0.29 - 0.384 4 -<br />
7 C 0.58 D 0.57 D 0.26 - 0.384 4 -<br />
<br />
''Key for output:''<br />
Number - residue number;<br />
Residue - amino-acid type;<br />
NORSnet - raw score by NORSnet (prediction of unstructured loops);<br />
NORS2st - two-state prediction by NORSnet; D=disordered;<br />
PROFbval - raw score by PROFbval (prediction of residue flexibility from sequence);<br />
Bval2st - two-state prediction by PROFbval;<br />
Ucon - raw score by Ucon (prediction of protein disorder using predicted internal contacts);<br />
Ucon2st - two-state prediction by Ucon;<br />
MD - raw score by MD (prediction of protein disorder using orthogonal sources);<br />
MD_rel - reliability of the prediction by MD; values range from 0-9. 9=strong prediction;<br />
MD2st - two-state prediction by MD.<br />
<br />
NORSnet, PROFbval and Ucon raw scores are the predicted tendencies of those methods and MD_raw is the final raw score by MD. In all 2-state scores, a residue is predicted to be disordered (D), if the raw score is higher than 0.5. We wrote an R script to plot all the raw scores for each protein.<br />
<br />
All the used scripts and created data can be found in: /mnt/home/student/kalemanovm/master_practical/Assignment3_SequenceBasedPredictions/Disorder.<br />
<br />
==Transmembrane Helices==<br />
The Memsat-SVM predictions were processed on the web server [http://bioinf.cs.ucl.ac.uk/psipred/ Psipred]. To get the membrane helices prediction I chose the option MEMSAT3 & MEMSAT-SVM instead of the default option.<br />
<br />
Polyphobius was run with the script provided in the subsection [https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php/Resource_software Software]. It can also be found on the rostlab server <code>/mnt/home/student/gerkej/gaucher/task3/tmh/polyphobius.pl</code>. It automatically includes <code>Blast</code> and <code>kalign</code><br />
<br />
==Signal Peptides==<br />
To find out more about signal peptides the following webservers were used:<br />
* [http://www.cbs.dtu.dk/services/SignalP/ SignalP]: version 4.1<br />
* [http://www.signalpeptide.de/index.php?m=listspdb_mammalia Signal Peptide Database]: last update, 6th November 2010<br />
<br />
On the Signal Peptide Database we also had a look on the transmembrane helices for the three proteins analyses in this exercise. Except of Glucocerbrosidase which does not have a documented membrane helix in the SP Database, the other three proteins of the "Membrane Helices" exercise are not listed in the Signal Peptide Database.<br />
<br />
==GO Terms==<br />
The GO Terms were reached by using the corresponding webservers of <br />
<br />
*[http://genius.embnet.dkfz-heidelberg.de/menu/cgi-bin/w2h-open/w2h.open/w2h.startthis?SIMGO=w2h.welcome GOPET]<br />
*[http://www.cbs.dtu.dk/services/ProtFun/ ProtFun 2.2 Server]<br />
*[http://pp-dev.informatik.tu-muenchen.de:8080/home PredictProtein]<br />
*[http://pfam.sanger.ac.uk/ Pfam 27.0] , last update, March 2013</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38902
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:26:33Z
<p>Kalemanovm: /* Which predictions can be improved considerably by structure-based approaches */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontology category indicates the location of the protein, which acts in the macrophages (immune system cells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
*This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties.<br />
<br />
*This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily.<br />
<br />
*The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover, biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accessibility<br />
Protein protein interactions <br />
*SNPs effect prediction</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38901
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:25:45Z
<p>Kalemanovm: /* Which predictions can be improved considerably by structure-based approaches */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontology category indicates the location of the protein, which acts in the macrophages (immune system cells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
*This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties.<br />
<br />
*This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily.<br />
<br />
*The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover, biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility<br />
*SNPs effect prediction</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38900
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:25:29Z
<p>Kalemanovm: /* What else can/is be predicted from protein sequence alone */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontology category indicates the location of the protein, which acts in the macrophages (immune system cells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
*This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties.<br />
<br />
*This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily.<br />
<br />
*The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover, biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38899
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:23:53Z
<p>Kalemanovm: /* Pfam */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontology category indicates the location of the protein, which acts in the macrophages (immune system cells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
*This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties.<br />
<br />
*This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily.<br />
<br />
*The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38898
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:22:17Z
<p>Kalemanovm: /* ProtFun2.0 */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontology category indicates the location of the protein, which acts in the macrophages (immune system cells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38897
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:19:29Z
<p>Kalemanovm: /* Predict Protein */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on polysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontalogy category indicates to the location of the protein, which acts in the macrophages (immunocells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38896
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:18:14Z
<p>Kalemanovm: /* GOPET */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task 01 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on ploysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontalogy category indicates to the location of the protein, which acts in the macrophages (immunocells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38895
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:16:57Z
<p>Kalemanovm: /* Lysosome-associated membrane glycoprotein 1 (P47863) */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualized results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task1 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on ploysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontalogy category indicates to the location of the protein, which acts in the macrophages (immunocells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38894
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:16:20Z
<p>Kalemanovm: /* Aquaporin 4 (P11279) */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the scores are even higher than for Serum albumin. The signal peptide consists of 28 following residues:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualised results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task1 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on ploysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontalogy category indicates to the location of the protein, which acts in the macrophages (immunocells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm
https://i12r-studfilesrv.informatik.tu-muenchen.de/wiki/index.php?title=Gaucher_Disease:_Task_03_-_Sequence-based_predictions&diff=38893
Gaucher Disease: Task 03 - Sequence-based predictions
2013-09-06T00:14:36Z
<p>Kalemanovm: /* Serum albumin (P02768) */</p>
<hr />
<div>== Secondary Structure ==<br />
In this task secondary structure of a protein is predicted using ReProf and compared to PsiPred prediction and DSSP structure assignment. <br />
<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
=== Evaluation results ===<br />
Evaluation results of Reprof against Psipred and DSSP are summarized in <xr id="secondary structure results"/>. A Reprof run was performed starting with the Psi-BLAST PSSM after a run against big_80 with 3 iterations and E-value cutoff 10E-10 (as described in the lab journal in the link above).<br />
<br />
<figtable id="secondary structure results"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! rowspan="2" style="background:#adceff;" | Query<br />
! colspan="4" style="background:#adceff;" | Precision PsiPred<br />
! colspan="4" style="background:#adceff;" | Precision DSSP<br />
|-<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
! style="background:#efefef;" | E<br />
! style="background:#efefef;" | H<br />
! style="background:#efefef;" | L<br />
! style="background:#efefef;" | Total<br />
|-<br />
!P10775 || 47.3 || 99.4 || 59.6 || 72.6 || 42.1 || 96.0 || 62.2 || 78.5<br />
|-<br />
!Q9X0E6 || 86.1 || 97.2 || 75.9 || 87.1 || 83.0 || 97.3 || 77.0 || 87.8<br />
|-<br />
!Q08209 || 87.5 || 74.3 || 86.0 || 82.2 || 70.5 || 75.9 || 88.9 || 78.8<br />
|-<br />
!P04062 || 88.4 || 96.8 || 76.2 || 83.6 || 80.0 || 84.1 || 86.3 || 83.3<br />
|-<br />
|}<br />
<center><small>'''<caption>''' Precision ( = number of matches / number of residues) of the ReProf prediction regarding the PsiPred prediction as well as the DSSP assignment.</caption></small></center><br />
</figtable><br />
<br />
=== Human Glucosylceramidase (P04062) ===<br />
==== Aligned view ====<br />
Aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for the Gaucher's disease protein, P04062, is shown below.<br />
<br />
Sequence: MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHT<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ----------------------------------------<span style="color:#00FF7F">E</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br><br />
Sequence: GTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLI<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: --<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: HRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIA<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGM<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>----<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: QYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>------<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: NRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
<br />
<!--Figures:--><br />
<figure id="uniprot_sec_str_P04062" ><br />
[[Image:uniprot_sec_str_P04062.JPG|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P04062 P04062]</caption>]]<br />
</figure><br />
<br />
<figure id="1OGS_sec_str_vmd" ><br />
[[Image:1OGS_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OGS 1OGS], visualized with VMD using "new cartoon" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
Here, we compare the secondary structure predictions and DSSP assignment for the protein sequence P04062 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
UniProt secondary structure annotation assigns residues into one of the three states: helix, strand or turn. The annotation might be unreliable, if no evidence on experimental level is available for the protein. However, the existence of our protein, P04062, was verified on protein level, therefore we can rely on the annotation to some extent. The UniProt secondary structure annotation for P04062 is shown in the image above. It is also included into the alignment in previous section, regarding both turns and positions not in one of the three states (helix, strand or turn) as loops. As one can see from the alignment, ReProf and PsiPred both predict one long helix and ReProf additionally two short helices before it (with 4 and 2 residues) near the beginning of the sequence. However, UniProt annotates only loops there (and DSSP has no assignment there). Altogether, the secondary structures look very similar, excluding small disagreements in the exact position and length of a segment or short segments not presented everywhere. The latter may be falsely predicted or assigned.<br />
<br />
'''PDB'''<br><br />
The PDB structure of our protein P04062, 1OGS, consists of two identical chains, A and B. From looking at the cartoon representation colored according to the secondary structures, one can see that each chain contains many alternating helices and sheets connected by loops. Beta barrel fold can be recognized and an extra beta sheet ring on the side of each chain. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P04062.<br />
<br />
<br clear="all"/><br />
<br />
=== Ribonuclease inhibitor (P10775) ===<br />
==== Aligned view ====<br />
This is the aligned view of the secondary structure predictions with ReProf and PsiPred, the DSSP assignment and the UniProt annotation for Ribonuclease inhibitor (P10775).<br />
<br />
Sequence: MNLDIHCEQLSDARWTELLPLLQQYEVVRLDDCGLTEEHCKDIGSALRANPSLTELCLRTNELGDAGVHLVLQGLQSPTCKIQKLSLQNCSLTEAGCGVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><br><br />
Sequence: PSTLRSLPTLRELHLSDNPLGDAGLRLLCEGLLDPQCHLEKLQLEYCRLTAASCEPLASVLRATRALKELTVSNNDIGEAGARVLGQGLADSACQLETLR<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: LENCGLTPANCKDLCGIVASQASLRELDLGSNGLGDAGIAELCPGLLSPASRLKTLWLWECDITASGCRDLCRVLQAKETLKELSLAGNKLGDEGARLLC<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: -<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: ESLLQPGCQLESLWVKSCSLTAACCQHVSLMLTQNKHLLELQLSSNKLGDSGIQELCQALSQPGTTLRVLCLGDCEVTNSGCSSLASLLLANRSLRELDL<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br><br />
Sequence: SNNCVGDPGVLQLLGSLEQPGCALEQLVLYDTYWTEEVEDRLQALEGSKPGLRVIS<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>----<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_P10775" ><br />
[[Image:uniprot_sec_str_P10775.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/P10775 P10775]</caption>]]<br />
</figure><br />
<br />
<figure id="2BNH_sec_str_vmd" ><br />
[[Image:2BNH(P10775)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=2BNH 2BNH], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta</caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence P10775 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein P10775 was verified on protein level, too. Therefore, we can rely on the annotation to some extent. The UniProt secondary structure annotation for P10775 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. The main differences occur in ReProf in the first half of the sequence: prediction of a helix, where a 3-residue long strand is predicted by the other sources, and a prediction of a longer helix after that, where a shorter helix and a short strand are predicted by the others. The first case occurs once more and the latter three more times. Overall, more helices are predicted by ReProf. In the PsiPred prediction, DSSP assignment and UniProt annotation for the secondary structures look altogether very similar: a sequence of alternating helices and strands separated by loops, sometimes with two short consequent strands. <br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein P10775, 2BNH, one can see that it has the typical hoof fold, with helices on the outer side and sheets in the inner side connected by loops from both sides. This fold supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Divalent-cation tolerance protein CutA (Q9X0E6) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MILVYSTFPNEEKALEIGRKLLEKRLIACFNAFEIRSGYWWKGEIVQDKEWAAIFKTTEEKEKELYEELRKLHPYETPAIFTLKVENVLTEYMNWLRESVL<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: -<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>---<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
<br />
<br />
<!--Images:--><br />
<figure id="uniprot_sec_str_Q9X0E6" ><br />
[[Image:uniprot_sec_str_Q9X0E6.png|700px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q9X0E6 Q9X0E6]</caption>]]<br />
</figure><br />
<br />
<figure id="1VHF_sec_str_vmd" ><br />
[[Image:1VHF(Q9X0E6)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1VHF 1VHF], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q9X0E6 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
The existence of the protein Q9X0E6 was also verified on protein level, thus we can rely on its UniProt annotation. The UniProt secondary structure annotation for Q9X0E6 is shown in the image above. Like before, it is also included into the secondary structure alignment in previous section. It is a short protein of only 101 amino acids and all 4 sources agree almost entirely in the secondary structure prediction or assignment. The protein contains three helices (apart from a single loop predicted in the second helix in PsiPred) and 4 strands according to PsiPred and DSSP. ReProf splits the second strand by a 3-residue loop, whereas UniProt splits the last strand by 2 loop residues.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q9X0E6, 1VHF, in Pymol we can see the number and consequence of its helices, loops and strands : lELHLELEHLELh (a lower case letter represents here one residue in this structure and an upper case letter multiple residues in this state). It is the same as the DSSP assignment of the protein P10775.<br />
<br />
<br clear="all"/><br />
<br />
=== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ===<br />
==== Aligned view ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
Sequence: MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: ----------------<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-------<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHG<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: GLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFP<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: ---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>-<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>-<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-<span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#FF1493">L</span>--<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>--<span style="color:#00FF7F">E</span>-<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><br />
UniProt: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><br><br />
Sequence: SLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKI<br />
ReProf: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br />
DSSP: <span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---<span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>---<span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span>---------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span>--<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span>-----------------------------<br />
UniProt: <span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#00FF7F">E</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: RAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSIN<br />
ReProf: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------------------------------------------------------<span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span>-------------------<br />
UniProt: <span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><span style="color:#4682B4">H</span><br><br />
Sequence: KALTSETNGTDSNGSNSSNIQ<br />
ReProf: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
PsiPred: <span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><span style="color:#FF1493">L</span><br />
DSSP: --------------------<br />
UniProt: <br><br />
<br />
<!--Images--><br />
<figure id="uniprot_sec_str_Q08209" ><br />
[[Image:uniprot_sec_str_Q08209.png|1000px|thumb|'''<caption>''' Schematic representation of UniProt secondary structure for [http://www.uniprot.org/uniprot/Q08209 Q08209])</caption>]]<br />
</figure><br />
<br />
<figure id="1AUI_sec_str_vmd" ><br />
[[Image:1AUI(Q08209)_sec_str_vmd.JPG|250px|thumb|'''<caption>''' Secondary structure of the PDB structure [http://www.rcsb.org/pdb/explore/explore.do?structureId=1AUI 1AUI], visualized with VMD using "licorice" representation. Helices: blue, beta strands and sheets: green, loops and turns: magenta </caption>]]<br />
</figure><br />
<br />
==== Comparison to available knowledge ====<br />
The following is the comparison of the secondary structure predictions and DSSP assignment for the protein sequence Q08209 to the available knowledge in UniProt and PDB.<br />
<br />
'''UniProt'''<br><br />
Also the last protein we explore was verified on protein level. Therefore we can trust the UniProt annotation to some extent. The UniProt secondary structure annotation for Q08209 is shown in the image above, which is also included into the secondary structure alignment in previous section. It is a long protein of over 500 residues. Some disagreements about its secondary structure assignment can be seen. According to all four sources, the protein contains many loops and helices and some strands. Like in P04062, there are some disagreements in the exact position and length of a segment or short segments not present everywhere. The main differences are: <br />
*A 6-residue long helix is assigned by DSSP and UniProt, but not by the predictors.<br />
*ReProf does not find a 4-6 residues long helix present at the three other sources. <br />
*ReProf predicts a 5-residues long strand instead of a 7-residues long helix assigned by DSSP and UniProt.<br />
*PsiPred predicts only a loop instead of the latter helix.<br />
*PsiPred predicts a helix, where a 4-6 residue long strand is assigned by the other sources.<br />
*Near the end of the protein (bevore the last "conserved" helix), RPsiPred predicts a long helix, ReProf only a 3-residues long helix, whereas only lopps are assigned by DSSP and UniProt in that region.<br />
<br />
'''PDB'''<br><br />
From the visualization of the PDB structure of the protein Q08209, 1AUI, one can see that it contains many helices connected by loop regions and sometimes by short strands, also there is a beta-sheet region consisting of ten strands. This supports our predictions, the DSSP assignment and the UniProt annotation of the secondary structure of the protein Q08209.<br />
<br />
=== Colclusion ===<br />
Using the secondary structure predictions ReProf and PsiPred, DSSP assignments and UniProt annotations, we could learn for our protein that its secondary structure mainly contains helices and strands. It is a dimer with each chain folding into a beta-barrel domain. Also about the example proteins, we could learn about their secondary structures (already discussed in the respective sections). <br />
<br />
To sum up, ReProf and PsiPred are good secondary structure prediction tool. Their predictions agree in the most cases with the DSSP assignment - typically used as a reference, because it uses the actually resolved PDB structure of a protein, which could also be seen in the visualization of the structures - and the UniProt annotation of the secondary structure.<br />
<br />
<br clear="all"/><br />
<br />
==Disorder==<br />
In this task we predict protein disordered and globular regions using IUpred and MetaDisorder.<br><br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
===IUPred===<br />
IUpred is a protein disorder predictor. User can choose one of the three options:<br />
* '''long''' for prediction of long disorders<br />
* '''short''' for prediction of short disorders<br />
* '''glob''' for prediction of structured, globular domains<br />
IUpred prediction results for each protein are presented and described in the plots in <xr id="iupred"/>. The disorder tendency ranges from 0 to 1 and is plotted for each residue in a protein sequence. Residues with a tendency above 0.5 are seen as disordered.<br />
<br />
<figure id="iupred" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.combined.iupred.png|IUpred results for protein '''P04062'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. Almost the whole protein sequence - from position 4 till the end (536) - is in a globular domain, according to "glob". Only a short region of three first residues is predicted to be disordered.<br />
File:P10775.combined.iupred.png|IUpred results for protein '''P10775'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (456 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q9X0E6.combined.iupred.png|IUpred results for protein '''Q9X0E6'''. There is almost no difference between the "long" and "short" prediction, the latter only predicts more disorder at the beginning and the end of the protein. The whole protein sequence (101 residues long) is predicted to be in a globular domain, according to "glob".<br />
File:Q08209.combined.iupred.png|IUpred results for protein '''Q08209'''. There are only small deviations between the "long" and "short" prediction, "long" predicts more disorder at the end on the protein, whereas "short" predicts more disorder at the beginning and the very end of the protein. According to "glob", a major part of the protein sequence - from position 5 till 446 from a total of 521 residues - is in a globular domain. Therefore, two disordered regions are predicted: one consisting only of four residues at the beginning and one containing 75 amino acids at the end of the protein.<br />
</gallery><br />
<small>'''<caption>''' IUpred predictions of disordered protein regions with the different options: long, short and glob.</caption></small><br />
</figure><br />
<br />
===MetaDisorder===<br />
MetaDisorder (MD) is a meta-predictor that combines several prediction methods:<br />
*'''NORSnet''': prediction of unstructured loops<br />
*'''PROFbval''': prediction of residue flexibility from sequence<br />
*'''Ucon''': prediction of protein disorder using predicted internal contacts <br />
Among the prediction scores of the three predictors, MD gives the final decision on disorder as well as MDrel: reliability of the final prediction, whose values range from 0-9 (9 is the strongest prediction). <br />
The raw prediction scores as well as the MD final score for each of the four proteins are visualized in <xr id="md"/>.<br />
<br />
<figure id="md" ><br />
<gallery widths="500px" heights="200px" perrow="2"> <br />
File:P04062.md.png|MetaDisorder results for protein '''P04062'''. MD prediction (red line) looks very similar to IUpred prediction, the very beginning of the protein is predicted to be disordered. The predictions of the single programs look very different and are very fluctuating. PROFbval (blue line) outputs higher scores (frequently over 0.5), than NORSnet (green line) and Ucon (purple line), however Ucon has some high peaks over 0.5. Overall, MD prediction seems more reliable, than predictions of the stand-alone methods.<br />
File:P10775.md.png|MetaDisorder results for protein '''P10775'''. MD results are very similar to IUpred "short" results, the very beginning and end of the protein seem to be slightly disordered, however the score goes only a little over 0.5 for this prediction to be reliable. PROFbval prediction is the most similar to MD because of the higher scores at the ends, only the scores are overall higher (often over 0.5). NORSnet and Ucon output lower scores and still lower at the ends, Ucon has sometimes high peaks. Again, MD seems to predict disordered regions better than the single programs.<br />
File:Q9X0E6.md.png|MetaDisorder results for protein '''Q9X0E6'''. Compared to IUpred prediction, MD, PROFbval and Ucon predictions look very different and fluctuating, predicting several disordered regions, which are not present in the IUpred results. Only NORSnet predicts the whole protein as lacking unstructured loops, like IUpred. Maybe the worse prediction of MD can be caused by the short length of this protein.<br />
File:Q08209.md.png|MetaDisorder results for protein '''Q08209'''. MD predicts disorder at the beginning and the end of the protein, as IUpred. NORSnet prediction is also similar, however it predict less disorder at the very beginning but more disorder after it (approx. position 5-40). Interestingly, both MD and NORSnet predict slight peaks around the position 240 and 375. The two other methods - Ucon and PROFbval - have very fluctuating prediction with higher scores and many peaks. Here MD and NORSnet seem to make more reliable predictions.<br />
</gallery><br />
<small>'''<caption>''' MetaDisorder (MD) predictions of disordered protein regions. Raw scores of the used programs: NORSnet, PROFbval and Ucon, as well as the MD score are shown.</caption></small><br />
</figure><br />
<br />
===DisProt===<br />
==== Human Glucosylceramidase (P04062) ====<br />
<figure id="P04062_disprot" ><br />
[[File:P04062_disprot_search.JPG|500px|thumb|'''<caption>''' DisProt Psi-BLAST search result for protein P04062.</caption>]]<br />
</figure><br />
<br />
For our protein, there is no entry in DisProt. There is an option to search for similar sequences in DisProt, with Smith-Waterman alignment method or Psi-BLAST. Using the Psi-BLAST search, only one sequence producing significant alignment was found: DP00159. As only a short region was aligned (47 aligned columns) and the sequence identity of the alignment is only 27%, we cannot consider DisProt annotation of the found protein DP00159 for the protein P04062.<br />
<br />
==== Ribonuclease inhibitor (P10775) ====<br />
Also for protein P10775 no DisProt entry is found. Psi-BLAST search yielded seven matches. The one with the best score and E-value is with the sequence DP00554. As the alignment is pretty good (40% identity and 196 aligned columns), we check the annotation of the similar protein [http://www.disprot.org/protein.php?id=DP00554 DP00554]. According to the annotation, there is only one 20 residues long disordered region near the beginning of the sequence (residues 31 - 50). However, this region does not fall into the alignment (aligned positions of the query are: 144 - 339 and of the target: 787 - 982). Therefore, also this DisProt annotation is useless for our protein of interest, P10775.<br />
<br />
==== Divalent-cation tolerance protein CutA (Q9X0E6) ====<br />
The protein Q9X0E6 is not found in DisProt as well. With Psi-BLAST search only insignificant and short matches are found, which cannot be considered for DisProt annotation transfer.<br />
<br />
==== Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (Q08209) ====<br />
It is the only protein from the four which could be found in DisProt with the ID.<br />
According to DisProt, there are five disordered regions in the protein, two of them overlaping (see the figure on the right and <xr id="Q08209_disprot"/>). These regions are at the ends of the sequence: one at the N terminus (positions 1 - 13) and four at the C terminus (altogether positions 374 - 521). The sixth region is ordered and is in the core of the sequence and is also the longest region (positions 14 - 373). (All regions map to the PDB structure [http://www.pdb.org/pdb/explore/explore.do?structureId=1AUI 1AUI]:A.)<br />
The predictions we made with IUpred an MD for the protein Q08209 yielded similar results for the disordered regions.<br />
<br clear=all><br />
<figure id="Q08209_disprot" ><br />
[[File:Q08209_disprot.png|800px|thumb|center|'''<caption>''' DisProt visualization of disordered and ordered regions for protein Q08209.</caption>]]<br />
</figure><br />
<br />
<figtable id="Q08209_disprot"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center" <br />
! style="background:#adceff;" | Region <br />
! style="background:#adceff;" | Type <br />
! style="background:#adceff;" | Name<br />
! style="background:#adceff;" | Location <br />
! style="background:#adceff;" | Length <br />
! style="background:#adceff;" | Structural/functional type<br />
! style="background:#adceff;" | Functional classes<br />
! style="background:#adceff;" | Functional subclasses<br />
|-<br />
| 1 || Disordered - Extended || || 1 - 13 || 13 || Relationship to function unknown || Unknown || Unknown<br />
|-<br />
| 2 || Disordered - Extended || || 374 - 468 || 95 || Function arises via a disorder to order transition || Molecular assembly || Autoregulatory, Protein-protein binding <br />
|-<br />
| 3 || Disordered - Extended || CaM-binding domain || 390 - 414 || 25 || Function arises via a disorder to order transition || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 4 || Disordered - Extended || Autoinhibitory region || 469 - 486 || 18 || Function arises via an order to disorder transition and vice versa || Molecular assembly || Protein-protein binding, Autoregulatory<br />
|-<br />
| 5 || Disordered - Extended || || 487 - 521 || 35 || Relationship to function unknown || Unknown || Unknown <br />
|-<br />
| 6 || Ordered || || 14 - 373 || 360 || || || <br />
|-<br />
|}<br />
<center><small>'''<caption>'''DisProt disordered and ordered regions for protein Q08209.</caption></small></center><br />
</figtable><br />
<br />
==Transmembrane Helices==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
Four Proteins, including the Gaucher disease causing Protein, where analyzed under reference by transmembrane (TM) helices. The used prediction tools differ in their analyzing features. While Polyphobius only differs between residues being part of a transmembrane helix (TMH) or being inside/outside of the cytoplama, Memsat-SVM also predicts re-entrant helices and pore-linig helices. Due to the fact that '''<span style="color:#045FB4">pore-lining helices</span>''' are also transmembrane helices, this kind of helices is detected of both prediction tools. In case of '''<span style="color:#a30909">re-entrant helices</span>''' both programs differ. In general a membrane helix crosses the membrane, so that both ends of the helix lie on different sides of the membrane. In contrast, the re-entrant helix leads bot its ends to the same side of the membrane. Memsat-SVM can predict re-entrant helices, but Polyphobius treats this helices as a general membrane helices, which crosses the membrane (seen for Q9YDF8), or ignores it (seen for P47863). In case of re-entrant helices predictions also the C-terminal or the N-terminal may be predicted on different membrane sides, as well as some helices may be predicted to lie in a different direction within the membrane, because of an re-entrant helix.<br />
<br />
The database OPM do not give the direct information about the localization of the N- and C-terminus as well as the type of the helices. Instead of differing between transmembrane and re-entrant helices, OPM classifies all identified membrane helices (MH) as transmembrane. These localizations and the helix type (transmembrane or re-entrant) can be detected from the visualization of the protein, provided by OPM and also shown in the following tables (<xr id="gluco"/>, <xr id="aero"/>, <xr id="lyso"/>, <xr id="dopa"/>). <br />
<br />
The second database PDMTM only contains transmembrane proteins. For non-transmembrane proteins, no information is available.<br />
<br />
===Human Glucosylceramidase===<br />
This protein is not a membrane protein and is located on the extracellular side of the membrane as documented in OPM. For the same reason there exist no entry in the PDBTM, as this database only contains membrane proteins. The prediction of Polyphobius causes to the same result. Additionally, Polyphobius predicted also the signal peptide (including the N/H/C-region). MemsatSVM detected a false positive transmembrane helix. As the Glucosylceramidase cleaves lipids of cell membranes, the active site of the enzyme may be mistaken for a transmembrane helix.<br />
<br />
<figtable id="gluco"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for '''Glucosylceramidase''' (P04062, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|1<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|align="center" |MH Topology<br />
|''' <span style="color:#045FB4"> 456-471</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
| align="center" | -<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|extracellular<br />
|align="center" | -<br />
|-<br />
|Signal peptide<br />
|'''1-34'''<br />
|'''1-40'''<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1</span>'''<br />
|align="center" | -<br />
|align="center" | -<br />
|align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P04062.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P04062.png|thumb|200x200px| ]]<br />
|[[Image:1ogs.png|thumb|200x200px| ]]<br />
|align="center" | -<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/09c2b58e-b998-11e2-abfa-00163e110593 P04062]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1OGS 1OGS]<br />
|1OGS is not in the PDBTM<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human glucocerebrosidase. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Aeropyrum pernix Voltage-gated potassium channel===<br />
For the protein of the Arachae, Aeropyrum pernix, 4 different PDB ids were found:<br />
*1ORQ: chain C<br />
*1ORS: chain C<br />
*2A0L: chain A/B<br />
*2KYH: chain A<br />
<br />
As all PDB entries represent structures of different chains, which are not the same, it was difficult to choose one of the entries. In the end the 1ORS was chosen for two reasons. This X-ray structure has the highest resolution compared to the others. Aside from this, this structure represents a sensor domain and must be important for the protein. <br />
The predictions have completely different results than the assignments. As the predictions are more similar to each other, they were compared to each other. The same was done for the two assignments. Both predictions have the same number of helices. Nevertheless, some helices have a greater deviation in their position. Memsat predicted an re-entrant helix where Polyphobius detected a transmembrane helix. That is why the N-terminus is predicted differently by both programs.<br />
<br />
The assignment of OPM has actually one helix more, but only because of a different declaration of its helices than PDBTM. The third helix of PDBTM consist of two shorter consecutive helices. Together they form one larger helix which crosses the membrane once and are therefore seen as one helix in PDBMT. These two mini-helices which would be too short to cross the membrane alone are counted separately in OPM. Apart from a light deviation of a few residues at the ends of the helices, the structure is the same in both databases.<br />
<br />
<figtable id="aero"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Voltage-gated potassium channel (Q9YDF8, Aeropyrum pernix)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MHs<br />
|6<br />
|7<br />
|5<br />
|4<br />
|-<br />
|align="center" |MH Topology<br />
| 1. '''43-59'''<br> 2. '''72-90'''<br> 3. '''101-118'''<br> 4. '''128-143'''<br> 5. '''163-184'''<br>6. ''' <span style="color:#a30909"> 188-217 </span>''' <br> 7. '''<span style="color:#045FB4"> 221-245</span>'''<br />
| 1. '''42-60'''<br> 2. '''68-88'''<br> 3. '''108-129'''<br> 4. '''137-157'''<br> 5. '''163-184'''<br>6. '''196-213'''<br>7. ''' 224-244'''<br />
| 1. '''25-46'''<br>2. '''55-78'''<br>3. '''86-97'''<br>4. '''100-107'''<br>5. '''117-148''' <br>6. -<br>7. -<br />
|1. '''27-50'''<br>2. '''55-75'''<br>3. '''88-107'''<br> 4. - <br>5. '''118-142''' <br>6. -<br>7. -<br />
|-<br />
|N-terminal<br />
|cytoplasmic<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|''' <span style="color:#a30909"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 1 </span>'''<br />
|''' align="center" | -'''<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:graphik_Q9YDF8.png|thumb|200x200px| ]]<br />
|[[Image:1ors-Q9YDF.png|thumb|200x200px| ]]<br />
|[[Image:1ors_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/e168825c-c55f-11e2-9aac-00163e110593 Q9YDF8]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=1ORS 1ORS]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/1ors 1ORS]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for aeropyrum pernix Voltage-gated potassium channel. Pore-lining helices are colored blue. Re-entrant helices are highlighted red.</caption></small></center><br />
</figtable><br />
<br />
===Human Lysosome-associated membrane glycoprotein 1===<br />
Both predictions have results similar to the assignments of OPM and PDBMT. All predicted transmembrane helices differ in their position only by a few residues. The protein consists of 6 transmembrane helices and 2 re-entrant helices. Polyphobius skips the re-entrant helices prediction but predicts the remaining membrane helices well. MemsatSVM predicts the re-entrant helices similar to the re-entrant helices of the database entries. Unfortunately MemsatSVM predicts the placing inside the membrane wrong. Instead of the C- and N-terminal situated in the cytoplasm, MemsatSVM places the both ends in the extracellular region. <br />
<br />
The two assignments are very similar, OPM does not particularly signs two of its helices as re-entrant but both helices can be seen as re-entrant in the OPM visualization. The re-entrant helices are colored gold in the PDBTM and are lightly silhouetted against the yellow transmembrane helices. All pictures can be found in <xr id="lyso"/>.<br />
<br />
<figtable id="lyso"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|8<br />
|6<br />
|8 (per chain)<br />
|8 (per chain)<br />
|-<br />
|align="center" |MH Topology<br />
|1. ''' <span style="color:#045FB4"> 35-56</span>'''<br>2. '''<span style="color:#045FB4"> 71-89</span>'''<br>3. '''<span style="color:#a30909"> 93-109</span>'''<br>4. '''113-136'''<br>5. '''<span style="color:#045FB4"> 157-178</span>'''<br>6. '''<span style="color:#045FB4"> 190-205</span>'''<br>7. '''<span style="color:#a30909">209-225 </span>'''<br>8. '''232-252'''<br />
|1. '''34-58'''<br>2. '''70-91'''<br>3. -<br>4. '''115-136'''<br>5. '''156-177'''<br>6. '''188-208'''<br>7. -<br>8. '''231-252'''<br />
|1. '''34-56'''<br>2. '''70-88'''<br>3. '''<span style="color:#a30909">98-107</span>'''<br>4. '''112-136'''<br>5. '''156-178'''<br>6. '''189-203'''<br>7. '''<span style="color:#a30909"> 214-223</span>'''<br>8. '''231-252'''<br />
|1. '''39-55'''<br>2. '''72-89'''<br>3. '''<span style="color:#a30909">95-106</span>'''<br>4. '''116-133'''<br>5. '''158-177'''<br>6. '''188-205'''<br>7. '''<span style="color:#a30909">209-222</span>'''<br>8. '''231-248''' <br />
|-<br />
|N-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-20'''<br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Re-entrant Helix<br />
|'''<span style="color:#a30909">2</span>'''<br />
| align="center" | -<br />
|'''<span style="color:#a30909">2</span>'''<br />
|'''<span style="color:#a30909">2</span>'''<br />
|-<br />
|Pore-lining Helix<br />
|''' <span style="color:#045FB4"> 4</span><br />
| align="center" | -<br />
| align="center" | -<br />
| align="center" | -<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P47863.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P47863.png|thumb|200x200px| ]]<br />
|[[Image:2d57.png|thumb|200x200px| ]]<br />
|[[Image:2d57_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/18e493a6-c560-11e2-9aac-00163e110593 P47863]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=2d57 2D57]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/2d57 2D57]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human Lysosome-associated membrane glycoprotein 1. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
===Human D3 dopamine receptor===<br />
The dopamine receptor is a transmembrane protein. All predicted transmembrane helices of the predictors and the databases agree mostly, with only a less shift of a few residues. While Polyphobius predicts all 7 transmembrane helices which are also documented in OPM and PDMTM, Memsat_SVM only identifies 6 transmembrane helices. As the missing helix is the last one, the C-terminus of the protein is localized as extracellular instead of cytoplasmic. The program classifies the 3rd helix as a pore-lining helix.<br />
<br />
<figtable id="dopa"><br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! colspan="5" style="background:#adceff;" | Comparison of membrane helices (MH) for D3 dopamine receptor (P35462, human)<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="2" style="background:#adceff;" | Prediction<br />
! colspan="2" style="background:#adceff;" | Assignment<br />
|-<br />
! style="background:#efefef;" align="center" | <br />
! style="background:#efefef;" align="center" | Memsat SVM<br />
! style="background:#efefef;" align="center" | Polyphobius<br />
! style="background:#efefef;" align="center" | OPM<br />
! style="background:#efefef;" align="center" | PDMTM<br />
|-<br />
|# of MH<br />
|6<br />
|7<br />
|7<br />
|7<br />
|-<br />
|align="center" |MH Topology<br />
|1. '''32-55'''<br>2. '''65-88'''<br>3. '''<span style="color:#045FB4"> 101-129</span>'''<br>4. '''151-169'''<br>5. '''188-209'''<br>6. '''331-354'''<br>7. -<br />
|1. '''30-55'''<br>2. '''66-88'''<br>3. '''105-126'''<br>4. '''150-170'''<br>5. '''188-212'''<br>6. '''329-352'''<br>7. '''367-386'''<br />
|1. '''34-52'''<br>2. '''67-91'''<br>3. '''101-126'''<br>4. '''150-170'''<br>5. '''187-209'''<br>6. '''330-351'''<br>7. '''363-386'''<br />
|1. '''35-52'''<br>2. '''68-84'''<br>3. '''109-123'''<br>4. '''152-166'''<br>5. '''191-206'''<br>6. '''334-347'''<br>7. '''368-382'''<br />
|-<br />
|N-terminal<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|extracellular<br />
|-<br />
|C-terminal<br />
|extracellular<br />
|cytoplasmic<br />
|cytoplasmic<br />
|cytoplasmic<br />
|-<br />
|Signal peptide<br />
|'''1-29'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Re-entrant Helix<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Pore-lining Helix<br />
|'''<span style="color:#045FB4"> 1</span>'''<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Graphical position<br />
|[[Image:cartoon_P35462.png|thumb|200x200px| ]]<br />
|[[Image:graphik_P35462.png|thumb|200x200px| ]]<br />
|[[Image:3pbl.png|thumb|200x200px| ]]<br />
|[[Image:3pbl_lm.png|thumb|200x200px| ]]<br />
|-<br />
|more information<br />
|[http://bioinf.cs.ucl.ac.uk/psipred/result/df400a7c-b998-11e2-a557-00163e110593 P35462]<br />
|<br />
|[http://opm.phar.umich.edu/protein.php?search=3pbl 3PBL]<br />
|[http://pdbtm.enzim.hu/?_=/pdbtm/3pbl 3PBL]<br />
|}<br />
<center><small>'''<caption>''' Summary and comparison of different membrane predictors (Polyphobius, Memsat-SVM) and databases (OPM, PDMTM) for the human D3 dopamine receptor. Pore-lining helices are colored blue. Re-entrant helices are highlighted red. </caption></small></center><br />
</figtable><br />
<br />
==Signal Peptides==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
<br />
For the following proteins, the signal peptides as well as its cleavage sites were predicted with SignalP:<br />
*Glucosylceramidase (P04062, human)<br />
*Serum albumin (P02768, human)<br />
*Aquaporin 4 (P11279, rat)<br />
*Lysosome-associated membrane glycoprotein 1 (P47863, human)<br />
<br />
The four eukaryotic proteins were also looked up in the Signal Peptide Database to compare the entry with the results of the prediction.<br />
<br />
===Glucosylceramidase (P04062)===<br />
<br />
For the Glucosylcerbrosidase, the prediction of SignalP differs from the database entry. <br />
<br />
In the database the protein has a signal peptide of 39 residues. A signal peptide is characterized with high hydrophobicity in its core region followed by the cleavage site [http://www.signalpeptide.de/index.php]. Especially the residues 18-23 and 27-34 indicate with its higher hydrohobicity to a signal peptide (green area in <xr id="sp_gluco"/>, 2).<br />
<br />
'''MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASG'''<br />
<br />
<br />
However, the prediction of SignalP results in no signal peptide. On the visualization of the different scores in <xr id="sp_gluco"/>, the green signal peptide score shows the most possible prediction for an signal peptide. The green line is higher for the first 39 residues than for the later residues. But the calculated D-score of the detected peptide lies with 0.37 below the threshold (0.5). The peptide is neglected as signal. These residues are not only defined as signal peptide by the database, but were also detected, with a light deviation, by the transmembrane helix predictors MemsatSVM (residues 1-34) and Polyphobius(residues 1-40). <br />
<br />
<br />
<figure id="sp_gluco"><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P04062_GLCM_HUMAN.png| SignalP result for P04062: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File:hydropathy_P04062.png| Hydrophobicity of all residues of the signal peptide of P04062 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Glucosylceramidase. </caption></small><br />
</figure><br />
<br />
===Serum albumin (P02768)===<br />
The signal peptide consists of residues 1-18 and is predicted of SignalP as well as documented in the Signal Peptide Databse<br />
<br />
'''MKWVTFISLLFLFSSAYS'''<br />
<br />
<xr id="sp_ser"/> shows a clear prediction of the signal peptide. A high S-core for the signal peptide region with a D-score of 0.85 far over the threshold.<br />
The cleavage side is predicted between the residue 18 and 19. The database shows a high hydrophobicity for the residues 6-14 which marks the region as signal peptide as well.<br />
<br />
<figure id="sp_ser" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P02768_ALBU_HUMAN.png| SignalP result for P02768: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P02768.png| Hydrophobicity of all residues of the signal peptide of P02768 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Serum albumin.</caption></small><br />
</figure><br />
<br />
===Aquaporin 4 (P11279)===<br />
For Aquaporin the Scores are even higher than for Serum albumin. The signal peptide consists of 28 residues as follows:<br />
<br />
'''MAAPGSARRPLLLLLLLLLLGLMHCASA'''<br />
<br />
The database shows a large hydrophobic region of 17 residues. At the end of the protein a transmembrane helix with a length of 23 residues ending in cytoplasm is documented in the Aquaporin 4 entry.<br />
The SignalP prediction gives a D-score of 0.95 for the detected signal peptide. The cleavage site is predicted between the residues 28 and 29 (ASA-AM). <xr id="sp_aq"/><br />
<br />
<figure id="sp_aq" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P11279_LAMP1_HUMAN.png| SignalP result for P11279: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score.<br />
File: hydropathy_P11279.png| Hydrophobicity of all residues of the signal peptide of P11279 in the Signal Peptide Database.<br />
</gallery><br />
<small>'''<caption>''' SignalP output and information of Signal Peptide Database of Aquaporin 4. </caption></small><br />
</figure><br />
<br />
===Lysosome-associated membrane glycoprotein 1 (P47863)===<br />
The rat protein has no entry in the Signal Peptide Database, as no signal peptide exists for it.<br />
The visualised results of the prediction show on the first sight, that the protein does not have a signal peptide. All scores are lower than 0.21, which is far below the threshold for signal peptides.<br />
<br />
<figure id="sp_ly" ><br />
<gallery caption="" widths="600px" heights="300px" perrow="2"><br />
File:sp_P47863_AQP4_RAT.png| SignalP result for P47863: The green line represents the signal peptide score. The higher the score the higher the probability of a residue being part of a signal peptide. A higher raw cleavage site score (C-score) marks the residue directly after the cleavage side. The blue line shows a combination of the C and S-score. The threshold is marked by a red dotted line.<br />
</gallery><br />
<small>'''<caption>''' SignalP output of Lysosome-associated membrane glycoprotein 1 </caption></small><br />
</figure><br />
<br />
==GO Terms==<br />
[[Task 03 - Lab Journal (Gaucher)|Lab journal]]<br />
<br />
Not very good prediction, depends a lot on what is known.<br />
<br />
===GOPET===<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | GOPET: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | Aspect<br />
! colspan="1" style="background:#adceff;" | Confidence in %<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
|-<br />
|GO:0016787 <br />
|F <br />
|98<br />
|hydrolase activity<br />
|-<br />
|GO:0004348 <br />
|F <br />
|97<br />
|glucosylceramidase activity<br />
|-<br />
|GO:0016798 <br />
|F <br />
|97<br />
|hydrolase activity acting on glycosyl bonds<br />
|-<br />
|}<br />
<br />
GOPET mainly focus on the activity of the Glucosycerebrosidase. The predicted GO Terms are less but correlate with the disease description in task1 as well as the description in Pfam.<br />
<br />
===Predict Protein===<br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="4" style="background:#adceff;" | Predict Protein: GO Terms for Glucocerebrosidase<br />
|-<br />
! colspan="1" style="background:#adceff;" | <br />
! colspan="1" style="background:#adceff;" | GO ID<br />
! colspan="1" style="background:#adceff;" | GO Term<br />
! colspan="1" style="background:#adceff;" | Reliability in %<br />
|-<br />
|Biological Process<br />
|GO:0005515<br />
|protein binding<br />
|70<br />
|-<br />
|Cellular Component<br />
|align="center" |-<br />
|align="center" |-<br />
|align="center" |-<br />
|-<br />
|Molecular Function<br />
|GO:0007040<br>GO:0006665<br>GO:0005975<br>GO:0008219<br>GO:0005976<br />
|lysosome organization<br>sphingolipid metabolic process<br>carbohydrate metabolic process<br>cell death<br>polysaccharide metabolic process<br />
|49<br>36<br>35<br>19<br>8<br />
|-<br />
|}<br />
<br />
The first three predicted Terms of the Molecular Function are confirmed by our knowledge about the process. The protein has no directly influence on cell death (GO:0008219), but is indirect involved as it processes the cell membrane of death blood cells. A participation on polysaccaride metabolic process is not known, as the glucosylcerebrosidase acts on fatty acids not on ploysaccarides. This GO Term is supposed to be wrong.<br />
<br />
===ProtFun2.0===<br />
<br />
'''Functional category''' <br />
Prob Odds<br />
Amino_acid_biosynthesis 0.035 1.593<br />
Biosynthesis_of_cofactors 0.182 2.528<br />
'''Cell_envelope => 0.504 8.262'''<br />
Cellular_processes 0.032 0.438<br />
Central_intermediary_metabolism 0.382 6.063<br />
Energy_metabolism 0.067 0.740<br />
Fatty_acid_metabolism 0.027 2.088<br />
Purines_and_pyrimidines 0.538 2.213<br />
Regulatory_functions 0.031 0.191<br />
Replication_and_transcription 0.126 0.471<br />
Translation 0.082 1.863<br />
Transport_and_binding 0.560 1.365<br />
<br />
'''Enzyme/nonenzyme''' <br />
Prob Odds<br />
'''Enzyme => 0.773 2.698'''<br />
Nonenzyme 0.227 0.318<br />
<br />
'''Enzyme class'''<br />
Prob Odds<br />
Oxidoreductase (EC 1.-.-.-) 0.083 0.399<br />
Transferase (EC 2.-.-.-) 0.228 0.660<br />
'''Hydrolase (EC 3.-.-.-) 0.272 0.859'''<br />
Lyase (EC 4.-.-.-) 0.045 0.961<br />
Isomerase (EC 5.-.-.-) 0.011 0.345<br />
Ligase (EC 6.-.-.-) 0.017 0.332<br />
<br />
'''Gene Ontology category'''<br />
Prob Odds<br />
Signal_transducer 0.054 0.251<br />
Receptor 0.027 0.158<br />
Hormone 0.001 0.206<br />
Structural_protein 0.002 0.087<br />
Transporter 0.024 0.222<br />
Ion_channel 0.018 0.307<br />
Voltage-gated_ion_channel 0.004 0.195<br />
Cation_channel 0.012 0.268<br />
Transcription 0.070 0.550<br />
Transcription_regulation 0.030 0.237<br />
Stress_response 0.085 0.962<br />
'''Immune_response => 0.153 1.804'''<br />
Growth_factor 0.005 0.376<br />
Metal_ion_transport 0.009 0.020<br />
<br />
The ProtFun Server classifies P0462 as an enzyme of the class Hydrolase. The predicted Gene Ontalogy category indicates to the location of the protein, which acts in the macrophages (immunocells). The functional category "cell envelope" declares the protein to interact with the cell membrane. This is right as the protein not only interacts but processes fatty acids of membranes.<br />
<br />
===Pfam===<br />
For the protein P04062 [http://pfam.sanger.ac.uk/family/PF02055.11 O-Glycosyl hydrolase family 30] was found on position 40-533. <br />
<br />
{| border="1" cellpadding="4" cellspacing="0" align="center"<br />
|-<br />
! colspan="2" style="background:#adceff;" | Identifiers<br />
|-<br />
|Symbol<br />
|Glyco_hydro_30<br />
|-<br />
|Pfam family<br />
|PF02055<br />
|-<br />
|Pfam clan <br />
|CL0058<br />
|-<br />
|InterPro<br />
|IPR001139<br />
|-<br />
|SCOP<br />
|1nof<br />
|-<br />
|SUPERFAMILY<br />
|1nof<br />
|-<br />
|OPM family<br />
|125<br />
|-<br />
|OPM protein<br />
|1ogs<br />
|-<br />
|CAZY<br />
|GH30 <br />
|-<br />
|}<br />
<br />
<br />
<br />
'''O-Glycosyl hydrolase family 30'''<br />
<br />
<br />
This family is a part of glycoside hydrolases known under the EC 3.2.1. Glycoside hydrolases includes a great number of enzymes that destroy glycosidic bonds between carbohydrates and other moieties<br />
<br />
This glycoside hydrolases has the clan (CL0058): Tim barrel glycosyl hydrolase superfamily<br />
<br />
The Pfam entry for the family PF02055 also mentions the human glucosylcerbrosidase as Gaucher disease causing.<br />
<br />
==Discussion==<br />
===Other available methods===<br />
<br />
{| border="1" cellpadding="5" cellspacing="0" align="center"<br />
|-<br />
! style="background:#adceff;" align="center" | Prediction of<br />
! style="background:#adceff;" align="center" | Tool<br />
|-<br />
! rowspan="4;" style="background:#efefef;" align="center" | secondary structure<br />
| [http://imtech.res.in/raghava/apssp/ APSSP: Advanced Protein Secondary Structure Prediction Server]<br />
|-<br />
| [http://www.biogem.org/tool/chou-fasman/ CFSSP: Chou & Fasman Secondary Structure Prediction Server]<br />
|-<br />
| [http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_gor4.html GOR IV]<br />
|-<br />
| [http://www.compbio.dundee.ac.uk/www-jpred/ Jpred3]<br />
|-<br />
! style="background:#efefef;" align="center" | disorder<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ DISOPRED2]<br />
|-<br />
! rowspan="6;" style="background:#efefef;" align="center" | transmembrane helices<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/ MEMSAT3]<br />
|-<br />
| [http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]<br />
|-<br />
|[http://www.predictprotein.org/ PredictProtein]<br />
|-<br />
|[http://www.sbc.su.se/~miklos/DAS/ DAS]<br />
|-<br />
|[http://www.enzim.hu/hmmtop/ HMMTOP]<br />
|-<br />
|[http://www.ch.embnet.org/software/TMPRED_form.html TMpred]<br />
|-<br />
! rowspan="9;" style="background:#efefef;" align="center" | signal peptides<br />
| [http://www.predisi.de/ PrediSi]<br />
|-<br />
| [http://phobius.sbc.su.se/poly.html Polyphobius]<br />
|-<br />
| [http://bioinf.cs.ucl.ac.uk/psipred/?memsatsvm=1 MemsatSVM]<br />
|-<br />
|[http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::sigcleave SIGCLEAVE]<br />
|-<br />
|[http://antheprot-pbil.ibcp.fr/signal_prediction.html ANTHEPROT]<br />
|-<br />
|[http://signalfind.org/ Signal Find Server]<br />
|-<br />
|[http://spd.cbi.pku.edu.cn/ SPD]<br />
|-<br />
|[http://gpcr.biocomp.unibo.it/cgi/predictors/spep/pred_spepcgi.cgi SPEPlip]<br />
|-<br />
|[http://bp.nuap.nagoya-u.ac.jp/sosui/sosuisignal/sosuisignal_submit.html SOSUIsignal]<br />
|-<br />
! rowspan="3;" style="background:#efefef;" align="center" | GO terms<br />
| [http://www.geneontology.org/ AmiGo]<br />
|-<br />
| [http://www.rcsb.org/pdb/home/home.do PDB]<br />
|-<br />
| [http://www.uniprot.org/ Uniprot]<br />
|- <br />
|}<br />
<br />
===What else can/is be predicted from protein sequence alone===<br />
*Fold recognition (profile based pGenTHREADER and rapid GenTHREADER)<br />
*Fold domain recognition (pDomTHREADER)<br />
*Protein domain prediction (DomPred)<br />
*Homology modelling (BioSerf v2.0)<br />
*Function prediction (eukaryotic function: FFPred v2.0) <br />
*Prediction of TM topology and helix packing (SVM-based MEMPACK)<br />
[http://bioinf.cs.ucl.ac.uk/psipred/ http://bioinf.cs.ucl.ac.uk/psipred/]<br />
*Cleavage site prediction<br />
*Ab initio structure prediction (not very succesfull, combinatorial problem, computational intensive, worse for longer sequences. Moreover biological molecules are not necesserily in the lowest energy comformation.)<br />
*Solvent accesibility<br />
*Metal binding sites, active sites<br />
*Protein protein interactions<br />
*SNPs effect prediction<br />
<br />
===Which predictions can be improved considerably by structure-based approaches===<br />
*Solvent accesibility</div>
Kalemanovm